1. Endogenous aryl hydrocarbon receptor promotes basal and inducible expression of tumor necrosis factor target genes in MCF-7 cancer cells
- Author
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Justin K. Tomblin, James Denvir, Gary Z. Morris, Nalini Santanam, Donald A. Primerano, Jackie Fletcher, Jun Fan, Inderjit Mehmi, Travis B. Salisbury, Estil Hurn, and Goran Boskovic
- Subjects
Small interfering RNA ,Polychlorinated Dibenzodioxins ,Biology ,Biochemistry ,Article ,Gene expression ,Basic Helix-Loop-Helix Transcription Factors ,Humans ,RNA, Small Interfering ,Transcription factor ,Pharmacology ,Regulation of gene expression ,Gene knockdown ,Superoxide Dismutase ,Tumor Necrosis Factor-alpha ,respiratory system ,Aryl hydrocarbon receptor ,Molecular biology ,Enzymes ,Gene Expression Regulation ,Receptors, Aryl Hydrocarbon ,Gene Knockdown Techniques ,Inactivation, Metabolic ,Cancer cell ,MCF-7 Cells ,Cancer research ,biology.protein ,Tumor necrosis factor alpha - Abstract
The aryl hydrocarbon receptor (AHR) is a ligand-activated transcription factor that upon activation by the toxicant 2,3,7,8 tetrachlorodibenzo-p-dioxin (TCDD) stimulates gene expression and toxicity. AHR is also important for normal mouse physiology and may play a role in cancer progression in the absence of environmental toxicants. The objective of this report was to identify AHR-dependent genes (ADGs) whose expression is regulated by AHR in the absence of toxicants. RNA-Seq analysis revealed that AHR regulated the expression of over 600 genes at an FDR
- Published
- 2014
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