1. Prevalence and characterization of uropathogenic Escherichia coli harboring plasmid-mediated quinolone resistance in a Tunisian university hospital.
- Author
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Sana F, Mabrouka S, Claudine Q, Faouzi SA, Ilhem BB, and Véronique D
- Subjects
- Adolescent, Adult, Aged, Aged, 80 and over, Child, Child, Preschool, Cluster Analysis, Escherichia coli Infections microbiology, Female, Genes, Bacterial, Hospitals, University, Humans, Infant, Male, Middle Aged, Molecular Epidemiology, Molecular Typing, Plasmids analysis, Prevalence, Tunisia epidemiology, Uropathogenic Escherichia coli classification, Uropathogenic Escherichia coli genetics, Uropathogenic Escherichia coli isolation & purification, Virulence Factors genetics, Young Adult, Anti-Bacterial Agents pharmacology, Drug Resistance, Bacterial, Escherichia coli Infections epidemiology, Quinolones pharmacology, Uropathogenic Escherichia coli drug effects
- Abstract
The prevalence of plasmid-mediated quinolone resistance (PMQR) determinants was investigated in a Tunisian collection of 300 uropathogenic Escherichia coli. PMQR genes were detected in 68 isolates (22.7%) as follows: aac(6')-Ib-cr (n=66), qnrB1 (n=3), qnrA6 (n=1), and qnrS1 (n=1). Three isolates carried the 2 determinants aac(6')-Ib-cr and qnrB1. aac(6')-Ib-cr was usually carried on IncF-type plasmids (n=47/60) and frequently associated with blaCTX-M-15 (n=60). Substitutions in gyrA and parC genes were detected in 57.5% of strains. A major cluster including 29 isolates was individualized, 28 of them belonged to the virulent ST131 clone. In our hospital, the high prevalence of PMQR in E. coli isolates was due to horizontal transfer but also to the spread of ST131 clone., (Copyright © 2014 Elsevier Inc. All rights reserved.)
- Published
- 2014
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