1. INNO-LiPA DNA line probe assay misidentification of M. smegmatis as Mycobacterium fortuitum complex.
- Author
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van den Broek T, Janssen NG, Hetem DJ, Bekers W, Kamst M, Fluit AC, van Ingen J, Kusters JG, and Rentenaar RJ
- Subjects
- Adult, Animals, Cattle, Diagnostic Errors, Humans, Lymphadenitis microbiology, Lymphadenitis pathology, Lymphadenitis therapy, Male, Microbial Sensitivity Tests, Molecular Diagnostic Techniques standards, Mycobacterium Infections, Nontuberculous microbiology, Mycobacterium Infections, Nontuberculous pathology, Mycobacterium Infections, Nontuberculous surgery, Mycobacterium fortuitum chemistry, Mycobacterium smegmatis chemistry, Reagent Kits, Diagnostic, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Treatment Outcome, Lymphadenitis diagnosis, Molecular Diagnostic Techniques methods, Mycobacterium Infections, Nontuberculous diagnosis, Mycobacterium fortuitum classification, Mycobacterium fortuitum genetics, Mycobacterium smegmatis classification, Mycobacterium smegmatis genetics
- Abstract
Seven weeks after being kicked in the face by a cow, a 34-year-old male patient developed a posttraumatic mycobacterial lymphadenitis. A rapidly growing mycobacterial isolate cultured from a surgically drained lymphadenitis pus specimen was identified as Mycobacterium smegmatis by matrix-assisted laser desorption/ionization mass spectrometry and a combination of ITS-, hsp65-, and 16S rRNA-DNA sequence analysis, but as Mycobacterium fortuitum complex using the commercial INNO-LiPA Mycobacteria v2 line probe assay. As it is unclear if the misidentification of this strain is an exception, more research is required., (Copyright © 2019 Elsevier Inc. All rights reserved.)
- Published
- 2019
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