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[Display omitted] • Caffeine intake does not alter working memory and general attention. • The placebo effect can influence the caffeine response due to the expectation. • Long-term exposure to caffeine may have an influence on the results. • Caffeine's influence may be greater in activities involving attention and movement. This study aimed to evaluate the effect of caffeine on cognitive parameters in premenstrual syndrome women of childbearing age undergoing caffeine withdrawal, observing the influence of cytochrome P450 enzyme isozyme 1A2 genotypes. This is a crossover study with 26 women (all participants) grouped according to genotype (17 AA and 9 AC/CC). The cognitive assessment was carried out before and after caffeine or placebo. A significant increase in attention was showed both after placebo and caffeine administration, however, no statistical difference was observed between caffeine and placebo groups. This occurred both for all participants and in the genotype groups. Working memory was improved for all participants, and in the AC/CC group after placebo ingestion; the same did not occur after caffeine intake. In the AA group, no significant changes were observed for placebo or caffeine. Caffeine does not promote effects on cognitive performance in premenstrual syndrome women after caffeine abstinence. [ABSTRACT FROM AUTHOR]

Kruppel-like Factors (KLF) are responsible for regulating many genes involved in physiological and pathological processes. They are characterized by three conserved zinc-fingers in the DNA-binding domain, wherein mutations could affect the binding efficiency and transcription regulation. This study aimed to perform bioinformatics analysis to determine the most deleterious non-synonymous variants in KLFs involved in cardiac development and diseases, and their effects over the protein structure and stability. Eight hundred and fifty non-synonymous variants were found in seven KLFs related to cardiac diseases. Seventeen algorithms were used to predict the effect of selected variants over the structure and function of seven KLFs. The Top3 variants were selected in each category of conserved and non-conserved residues in the zinc-finger (ZF) domain. KLF5 p.Cys410Phe was the only variant predicted as deleterious in all algorithms, occurring in a conserved residue of zinc ion interaction. KLF15 p.Arg364Pro was the only variant predicted to affect the DNA-binding, and also occurs in a conserved ZF-domain. Our bioinformatics analysis determined potential variants that may lead to development of cardiac diseases, as well as reinforced the importance of KLF analysis in vitro and in vivo . [ABSTRACT FROM AUTHOR]