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[Display omitted] • 1H NMR metabolic profile for free-range and barn-raised broilers. • Screening t -test ranking showed significant differences in metabolite concentration. • Free-range broilers have more anserine and carnosine and less amino acids. • A univariate discriminant model based on the ratio of two NMR peaks was built. • This model showed 100% of correct classification and is more robust than PLS-DA. Intensive systems of raising chickens in barns prevail worldwide for financial reasons. In contrast, free-range chickens are raised in better welfare conditions, and preferred by consumers due to their distinctive taste/flavor, having higher market prices. Thus, free-range chickens have been the target of frauds. In this study, 1H NMR metabolic profiles of breasts of free-range and barn-raised broilers (108 individuals) were compared by two discriminant models, based on t -test ranking and partial least squares (PLS-DA). Both models provided 100 % of correct classification in both training and test sets, being the univariate model based on t -test screening simpler and more robust. Among other differences, barn-raised broilers presented lower carnosine and anserine concentrations, and higher free amino acids contents. Univariate discrimination was based on the ratio of two NMR signals assigned to β-alanine and carnosine + anserine, respectively. As an additional advantage, this profiling method could be adapted to other measurement platforms. [ABSTRACT FROM AUTHOR]

[Display omitted] • A simple single-step extraction method was developed for 1H NMR analysis of eggs. • 1H NMR lipidic profiles of free-range and conventional chicken eggs were compared. • Eggs from free-range chickens had higher concentrations of ω-3 and ω-6 PUFAs. • Free-range eggs showed a ratio between the signals at δ 2.85 and 4.14 of >0.14. • Free-range eggs had higher concentrations and similar amounts of cholesterol. The conventional intensive system produces cheap and safe chicken eggs, but exposes the animals to stress due to overcrowding on farms. This work compared the 1H NMR lipidic profile of chicken eggs produced in conventional and free-range systems. Sample preparation consisted of a single-step extraction and centrifugation, and the 1H NMR experimental time was just 3 min per sample. Eggs from free-range chickens had higher concentrations of ω-3 and ω-6 polyunsaturated fatty acids. The ratio between the signals at δ 2.85 and 4.14 from bis-allylic polyunsaturated fatty acids and glycerol moiety, respectively, was able to correctly classify 93.8 % of the samples. These results were similar to those of PLS-DA, used for comparative purposes. Therefore, the proposed method could be easily used to assist quality control and fraud prevention in the egg industry. Free-range eggs had higher concentrations of cholesterol but, as they are smaller, similar amounts to conventional ones. [ABSTRACT FROM AUTHOR]