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• Panel data of 285 Chinese cities and Tapio method are applied in decoupling analysis. • China's cities are generally facing deteriorating decoupling states of MSW. • N-shaped EKC hypothesis of MSW is established in eastern and central China. • Inverted N-shaped EKC hypothesis of MSW is established in western China. • Most cities still have to face more pressure brought by MSW in the next years. This study uses the Tapio elastic decoupling analysis method and an empirical model of the environmental Kuznets curve (EKC) to analyze the decoupling between municipal solid waste (MSW) generation and economic development in 285 of China's cities from 2002 to 2017. The decoupling analysis results show that the decoupling states in China's cities generally improved first and then deteriorated in 2002–2017. The proportion of cities experiencing deterioration of decoupling states had increased to 60.00% by 2014–2017, and cities with a higher economic development level generally had more serious deterioration. The empirical test results support the N-shaped EKC hypothesis of MSW, which can explain why the decoupling state changed from improvement to deterioration. The difference of the economic level of sample cities (which determines their position on EKC) can also explain the spatial heterogeneity of the decoupling state and its changes; for example, the proportion of cities that have crossed the inflection point of Tapio decoupling elasticity (the per capita GDP is 13,130.89 yuan) to enter the deterioration stage is highest (100/101) in the economically developed eastern region, and is lowest (66/75) in the economically backward western region of China. In general, the empirical results of EKC can effectively support and explain the results of decoupling analysis. [ABSTRACT FROM AUTHOR]

Abstract: Recent research indicates that miR-26a is involved in various biological processes including cell cycle, apoptosis and Hepatocellular carcinoma (HCC). In present report, we demonstrate the construction of recombinant plasmid miR-26a expression vector and its stable expression of miR-26a in transfected human liver cancer cells (HepG2). The double strands oligo encoding the miR-26a was designed and synthesized to generate the mature miR-26a expression plasmid. Use of the vectors in mammalian cells permits visual detection of cells expressing the pre-miRNA through co-cistronic expression of EGFP. The positive clones were screened by restriction enzyme digestion and sequenced. The new expression vector of miR-26a was named pHsa-miR-26a. PHsa-miR-26a and its controls were transfected to HepG2 cells. Fluorescence detection displayed that fluorescence intensity of GFP was highest during 48 and 72hours post-transfection, and qRT-PCR showed a significantly increase in miR-26a expression in the vector transfected cells compared with the expression in its controls. The recombinant plasmid expression vector of miR-26a was constructed successfully, which may facilitate further study of its function in the process of cell cycle and HCC. [Copyright &y& Elsevier]