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With the development and progress of the Internet era, the mode and development environment of many industries are gradually changing and developing towards networking, the combination of online and offline has become the mainstream, but the development of the Internet is accompanied by hidden dangers of personal information security, especially for the financial industry, industry has become a very prominent user experience problem in the Internet finance industry. With the prosperity of Internet finance, many enterprises attach importance to the construction of Internet Finance websites, and improve the trust and experience of users through the design of websites. This paper analyzes the user trust experience and trust mechanism in Internet financial websites; Through the analysis of different user groups to compare the degree of trust in Internet Finance websites, compare the level of trust and coupling between traditional finance and Internet Finance in the hearts of users; Through the research, it is found that most white-collar social workers have high trust in Internet financial websites, and the elderly group has the lowest trust; In contrast to traditional finance, we can effectively improve the functionality and value of financial products in the Internet Finance website, drive the emotional identity of users, and attract users visually from the website design, establishing and maintaining users' trust in Internet finance industry websites can greatly enhance users' trust in Internet finance; It expands the scope of financial services and simplifies service channels,it will have a positive impact on the development of Internet Finance and website design in the future. [ABSTRACT FROM AUTHOR]

Polypeptides with finger-like structures can often intercalate into the grooves of DNA, thereby affecting DNA repair or activating gene transcription, both of which are crucial for the regulation of physiological processes. Their conserved amino acid sequence and simple structure have provided useful elements for the design and assembly of functional molecules. In this paper, using the C 2 H 2 zinc finger domain and the PEP-FOLD3 online simulation platform 11 polypeptides containing 22 amino acid residues were designed. In addition, the CD spectroscopy was combined with the fluorescence spectroscopy to study the polypeptide structures and their interaction with DNA. Results showed that although addition of zinc ions affected the polypeptide structure, particularly of the polypeptides A4, B1, and B3, zinc ion was not an essential factor for increasing polypeptide-DNA interactions. Our study revealed an increase in the interaction strength between mutated polypeptides and DNA, suggesting that mutations disrupt polypeptide structure, and polypeptides interact with DNA by groove and electrostatic binding. Mutations at the 12th and 15th amino acid residues had the greatest effect. The stronger binding between A2 or B2 and DNA indicates that the polypeptide has a spatial structure that can stably interact with DNA. The structure and characteristics of these polypeptide domains can provide information for the design and development of new polypeptide functional molecules, which could have potential significance and applications. However, this information also suggests that there are many challenges facing polypeptide design due to the synergistic effects between the side chains of amino acid residues. Unlabelled Image • Structures of mutants are simulated vis PEP-FOLD3. • Zn2+ and DNA binding of finger-like peptides are analyzed using CD spectroscopy. • Key amino acid residues have the greatest effect on peptide structure. [ABSTRACT FROM AUTHOR]

Nicotinamide–modified peptides bind to ctDNA via groove and electrostatic binding mode. [Display omitted] • Development of new, targeted DNA peptide drugs. • The interaction forces and binding modes were determined via multispectroscopic. • Nicotinamide modification enhanced the binding affinity of parent peptide. Various peptide drugs have entered the market with the development of molecular biology. Peptide drugs are used for treat diseases such as diabetes, breast cancer, and HIV infection. In this study, three nicotinamide–modified peptides were synthesized by modifying the N–terminus of BRCA1 (856–871, Y856R, K862Y, R866W) peptide with three nicotinic acid derivatives using solid–phase peptide synthesis. The results of calf thymus DNA (ctDNA) binding activity indicated that binding constants of BRCA1 (856–871, Y856R, K862Y, R866W) (P0) and three nicotinamide–modified peptides (P1, P2, and P3) to ctDNA were 1.89 × 103, 2.97 × 104, 7.61 × 104, and 8.09 × 104 L·mol−1, respectively. The binding affinity of the modified peptides was superior to that of BRCA1 (856–871, Y856R, K862Y, R866W). ΔHθ < 0 and ΔSθ < 0 indicated that van der Waals force and hydrogen bond contributed most to peptide–ctDNA binding. Results obtained by Circular dichroism (CD) indicated that peptide binding interaction led to conformational changes in ctDNA. Ultraviolet–visible (UV) spectroscopy, ethidium bromide (EB) competition experiments, DNA melting experiments, and viscosity measurements verified that peptides interacted with ctDNA via groove binding. Ionic strength experiments manifested that electrostatic binding was also involved in peptide–ctDNA binding. [ABSTRACT FROM AUTHOR]

A novel restricted access materials (RAM) combined to molecularly imprinted polymers (MIPs), using malathion as template molecule and glycidilmethacrylate (GMA) as pro-hydrophilic co-monomer, were prepared for the first time. RAM-MIPs with hydrophilic external layer were characterized by scanning electron microscopy and recognition and selectivity properties were compared with the restricted access materials-non-molecularly imprinted polymers (RAM-NIPs) and unmodified MIPs. RAM-MIPs were used as the adsorbent enclosed in solid phase extraction column and several important extraction parameters were comprehensively optimized to evaluate the extraction performance. Under the optimum extraction conditions, RAM-MIPs exhibited comparable or even higher selectivity with greater extraction capacity toward six kinds of organophosphorus pesticides (including malathion, ethoprophos, phorate, terbufos, dimethoate, and fenamiphos) compared with the MIPs and commercial solid phase extraction columns. The RAM-MIPs solid phase extraction coupled with gas chromatography was successfully applied to simultaneously determine six kinds of organophosphorus pesticides from honey sample. The new established method showed good linearity in the range of 0.01–1.0 μg mL −1 , low limits of detection (0.0005–0.0019 μg mL −1 ), acceptable reproducibility (RSD, 2.26–4.81%, n = 6), and satisfactory relative recoveries (90.9–97.6%). It was demonstrated that RAM-MIPs solid phase extraction with excellent selectivity and restricted access function was a simple, rapid, selective, and effective sample pretreatment method. [ABSTRACT FROM AUTHOR]