Search

Objective: To investigate the correlation of circulating long non-coding RNA nuclear-enriched abundant transcript 1 (lncRNA NEAT1) expression with disease risk, severity, prognosis and inflammatory cytokine levels in sepsis patients.Methods: 152 sepsis patients and 150 health controls (HCs) were enrolled in this study. Plasma and serum samples were obtained from sepsis patients and HCs, and lncRNA NEAT1 expression in plasma was determined by quantitative polymerase chain reaction, while levels of inflammatory cytokines in serum were detected by enzyme linked immune sorbent assay.Results: LncRNA NEAT1 expression was remarkably higher in sepsis patients than in HCs (P < 0.001). Receiver operating characteristic (ROC) curve disclosed a good predictive value of lncRNA NEAT1 expression for sepsis risk with area under curve (AUC) of 0.730 (95% CI: 0.740-0.861). Subsequent multivariate logistic regression analysis demonstrated that lncRNA NEAT1 expression was independently associated with higher sepsis risk (P < 0.001). In sepsis patients, lncRNA NEAT1 expression was also observed to be positively correlated with Acute Physiology and Chronic Health Evaluation (APACHE) II score (P < 0.001), serum tumor necrosis factor-α (P < 0.001), interleukin (IL)-1β (P = 0.043), IL-6 (P = 0.001) and IL-8 (P = 0.038), while negatively correlated with IL-10 (P < 0.001). In addition, lncRNA NEAT1 expression was increased in non-survivors compared to survivors (P = 0.006), and ROC curve revealed a good prognostic value of lncRNA NEAT1 for non-survivor risk with AUC 0.641 (95% CI: 0.536-0.746).Conclusion: Circulating lncRNA NEAT1 correlates with increased disease risk, elevated severity and unfavorable prognosis as well as higher expression of pro-inflammatory cytokines in sepsis patients. [ABSTRACT FROM AUTHOR]

• Construct validity of the teacher version of the Children's Behavior Questionnaire-Very Short Form (CBQ-VSF-TF) examined with Mexican teachers of Mexican preschoolers. • New factor emerges likely representing child self-regulation through self-soothing. • Caution in use of CBQ for populations that differ from norming sample. The purpose of this study was to examine the construct validity of the teacher version of the Children's Behavior Questionnaire-Very Short Form (CBQ-VSF-TF)-a measure of temperament-with a sample of low-income Latinx preschool children. Forty-three teachers (95.3% female; 88.4% Latinx) of 247 preschool children completed the CBQ-VSF-TF. The development sample was primarily Caucasian and lower middle class. Results of our confirmatory factor analyses (CFA) with a sample of Latinx low-income children suggested a six-factor solution, whereas Allan et al. (2013) who adapted the parent version of CBQ-VSF to create the teacher version found a five-factor structure for CBQ-VSF-TF in their exploratory factor analyses. While in our CFA analyses we were able to largely replicate the five factors for CBQ-VSF-TF in Allan et al. (2013) , the items that loaded on the Effortful Control, Sensitivity, and Shyness factors were different in our CFA analyses versus those in the EFA analyses of Allan et al. (2013) , and we found an additional factor likely related to cultural perceptions in the teacher student match. Results of this study suggest caution in the use of the CBQ-VSF-TF as differences in the dimensionality of the CBQ-VSF-TF can emerge from its use with populations or raters that differ from development sample. [ABSTRACT FROM AUTHOR]