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Abstract: Meat is an integral part of the human diet. Besides essential amino acids and nutritive factors of high quality and availability, meat provides often overlooked components of importance for human health. These are amino acids and bioactive compounds that may be very important in i) preventing muscle wasting diseases, such as in sarcopenia, ii) reducing food and caloric intake to prevent metabolic syndrome, iii) blood pressure homeostasis via ACE-inhibitory components from connective tissue, and iv) maintaining functional gut environment through meat-derived nucleotides and nucleosides. In addition, meat could be an important source of phytanic acid, conjugated linoleic acids and antioxidants. Further, it becomes increasingly apparent that design of in vitro meat will be possible, and that this development may lead to improved health benefits from commercially viable and sustainable meat products. [Copyright &y& Elsevier]

Abstract: One factor affecting meat quality is pre-slaughter stress. We investigated the effects of exercise stress on drip loss and toughness in relation to resting times of 0, 1 or 3h following exercise on a treadmill. This exercise stress was regarded as combined physical and physiological stress. Exercise stress increased the muscle temperature, reduced the creatine phosphate, ATP and glycogen content of pigs slaughtered immediately after stress exposure. These conditions lead to a reduced pH early post mortem and an increased drip loss, while only 1h of rest after exercise stress normalised these effects. However, an overshooting effect was noted when pigs were rested for 1–3h before slaughter, emphasising the importance of critical control of the resting period when studying exercise stress-induced effects on meat quality. Furthermore, meat from exercise stressed pigs, irrespective of resting, had increased toughness compared to controls, indicating that the toughness was not related to drip loss in meat from exercise stressed pigs. [Copyright &y& Elsevier]

Abstract: Duroc and Landrace pigs as well as primary myotubes from these breeds were used to investigate mechanisms behind differences in their response to creatine monohydrate (CMH). Pigs were supplemented with 0, 12.5, 25 or 50g CMH/d for 5 days (n =10 per treatment and breed). Plasma levels of creatine increased dose-dependently in both breeds, while muscle-creatine phosphate content increased only in the Duroc pigs. 1H NMR metabolic profiling showed a tendency towards clustering according to CMH supplementation only among Duroc pigs, revealing a stronger response compared to Landrace pigs. The abundance of insulin-like growth factor I and myostatin mRNA was decreased by CMH supplementation while that of type 1 IGF-receptor and creatine transporter was unaffected. Protein synthesis, increased in the myotubes from both breeds, indicating protein accretion, but no effect was observed on the mRNA abundance of IGF-I, type 1 IGF-receptor, myostatin or the creatine transporter in myotubes. [Copyright &y& Elsevier]

Abstract: Increased creatine content in the muscle may delay post mortem lactate formation and postpone the pH decline, hence potentially improving the water-holding capacity (WHC). Duroc and Landrace pigs were supplemented with 0, 12.5, 25 or 50g creatine monohydrate (CMH)/d for 5 days prior to slaughter. Meat from Longissimus dorsi (LD) of Duroc pigs had a higher WHC and pH at all times, lower colour determinants; a* (redness), b* (yellowness), L* (lightness) and was more juicy compared to that of Landrace pigs. Furthermore, higher pH2h, pH24h and decreased colour determinants were observed in carcass sides exposed to a faster cooling profile. Dietary supplementation with CMH increased the body weight gain of both breeds. However, only meat from Duroc pigs had higher pH30min and pH45min (at 50g CMH/d) and WHC, but reduced redness (reduced in both breeds) and juiciness when supplemented with CMH compared to non-supplemented controls. [Copyright &y& Elsevier]

ABSTRACT In commercial production, chickens are subjected to feed withdrawal prior to slaughter and exposed to stress during transport and handling of the animals at the slaughterhouse; this causes plasma glucose and glycogen stores in liver and muscle to decrease, which has a negative impact on meat quality. The aim of the present study was to investigate how supplementation of the energy complements creatine and pyruvate during the fasting period would affect postmortem pH decrease, water-holding capacity, and color of the meat. Female Ross 208 broilers were supplemented with glucose combined with either pyruvate or creatine via the drinking water for 18 or 42 h prior to slaughter, i.e., before and throughout the fasting period. Chickens were slaughtered at 42 or 43 d of age. Temperature and pH were measured at 1, 10, 30, and 45 min and 1, 2, 4, 8, and 12 h postmortem. The results showed that the pyruvate and glucose supplementation increased the pH at 45 min postmortem by 0.25 units and decreased drip loss of musculus pectoralis major (PM) by 50 to 65% in chickens supplemented for 42 h. The creatine and glucose supplementation reduced pH at 3 and 4 h postmortem by 0.32 to 0.42 units, increased the lightness (L*) by 2.3 to 5.6 units, and increased drip loss by 51 to 137% in the PM of chickens supplemented for 18 and 42 h. Pyruvate and glucose supplementation thus appear beneficial but whether this is concomitant with an overall improvement in meat quality remains to be determined. [ABSTRACT FROM AUTHOR]

Discusses results of an experiment conducted on chickens that were fed a diet supplemented with a combination of ascorbic acid and alpha-tocopherol or oregano in order to ameliorate a negative effect of stress on meat quality characteristics. Tocopherol content in feed and muscle; Carotenoid content in oregano; Erythrocyte stability; Effect on activities of antioxidative enzymes.

Abstract: Nuclear magnetic resonance (NMR)-based metabonomics was applied to investigate the effects of pre-slaughter exercise stress on the plasma metabolite profile at time of slaughter. The study included a total of 40 slaughter pigs, which were exposed to one of the following treatments: No pre-slaughter stress (control treatment), pre-slaughter exercise on a treadmill and subsequently 0, 1, or 3h rest prior to slaughter. NMR-based metabonomics revealed a clear difference in the plasma metabolite profile at time of slaughter between control pigs and pigs exercised without rest, which mainly could be ascribed to increased plasma lactate due to exercise. A resting period of 1 or 3h prior to slaughter reversed the stress-induced perturbations in the plasma metabolite profile. The plasma metabolite profile at time of slaughter was highly correlated with muscle temperature 1min post-mortem, and a correlation to WHC was also demonstrated. Lactate was found to be the metabolite of importance for the association between the plasma metabolome and pH, temperature and WHC. [Copyright &y& Elsevier]

Abstract: Creatine content in the muscle may delay postmortem lactate formation and postpone the pH decline, hence potentially improving the water-holding capacity (WHC) as shown in a previous study including purebred Duroc pigs, although the same study did not find any effect on meat from purebred Landrace pigs. In the present study Danish D(LY) crossbreeds were supplemented with 0 or 50g creatine monohydrate (CMH)/d for five days prior to the slaughter. CMH supplementation had no effect on meat quality indicators (pH and temperature), meat quality attributes (WHC and colour) or eating quality (juiciness and tenderness) of meat from the D(LY) crossbred pigs. As a consequence the D(LY) crossbreed was classified as a non-responder to CMH supplementation. [Copyright &y& Elsevier]

The present experiment investigated the effects of a calcium deficient diet on the use of outdoor areas, foraging activity and production parameters in two strains of slow-growing broilers (i657 and a Labresse cross). The birds were housed as day-old in an indoor system of 2×8 groups of each 111 birds (11.8 birds/m2). After 6 weeks, the groups were moved to an outdoor housing system of 2×8 houses (3 m×5 m) with free access to outdoor grass-covered areas (9 m×22 m). The outdoor areas were equipped with two supported sloping roofs and a sandpit. In the outdoor system, the birds were fed one of the two feeds; high (1.1%) or low (0.3%) calcium, ad libitum in a 2×2 factorial design with four replicates. In each group, number and behaviour of birds outside were recorded 1 day per week between sunrise and sunset (15 scan samples per group per day) during a 6-week period until slaughter at 84 days of age. The number of birds in the outdoor area and the proportion of these being inactive was unaffected by the treatment. Surprisingly, birds on the high calcium diet showed more foraging activity when outside (P=0.04), and these birds also consumed more pelleted feed (P=0.01) and consequently had a larger body weight at slaughter (P=0.01). Birds on both treatments showed a diurnal activity rhythm and stayed more in the zones closest to and furthest from the house. In conclusion, the deprivation of calcium diminished foraging activity, feed intake and growth rate of the broilers. [Copyright &y& Elsevier]