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[Display omitted] • A method for monitoring administered AGs in various animal-derived foods is developed. • PVA-NFsM achieves effective extraction of targets as a new SPE adsorbent. • PVA NFsM shows good purification ability for sample matrix and reusability. • The accuracy and sensitivity of the method are equivalent to or better than existing methods. • The good practicality of the developed method was verified by testing actual samples. This work aims to develop a widely applicable method to monitor administered AGs in various animal-derived food samples to ensure food safety. A polyvinyl alcohol electrospun nanofiber membrane (PVA NFsM) was synthesized and employed as solid-phase extraction (SPE) sorbent, in combination with UPLC-MS/MS, for the simultaneous detection of ten AGs in nine types of animal-derived food samples. PVA NFsM exhibited excellent adsorption performance for the targets (with an adsorption rate of over 91.09%), good matrix purification ability (with a reduction of 7.65%-77.47% in matrix effect after SPE), and good recyclability (can be reused 8 times). The method displayed a linear range of 0.1–25000 μg/kg and attained limits of detection for AGs were 0.03–15 μg/kg. Spiked samples demonstrated a recovery of 91.72%-100.04% with a precision of<13.66%. The practicality of the developed method was verified by testing multiple actual samples. [ABSTRACT FROM AUTHOR]

• A novel dynamic adsorption sensing mode of tetracycline was developed for the first time. • SP@COF/SDBS/PS NFM was prepared as a new solid-phase sensor. • SP modified covalent organic framework was developed as a reference fluorescence for ratiometric fluorescence sensing. • Sensitive fluorescence sensing and fine colorimetric visualization detection of tetracycline in real sample was realized. • The sensing mechanism and characteristics were systematically evaluated. Sensitive and real-time tetracycline (TC) monitoring method is of great significant for food safety and human health. Herein, a novel TC dynamic sensing mode based on a solid-phase nanofiber mat (NFM) sensor SP@COF/SDBS/PS NFM was constructed for the first time, where spiropyran-modified covalent organic framework (SP@COF) with red emissions (λ em = 620 nm) was used as the reference fluorescence. Utilizing the adsorptive interaction between SP@COF/SDBS/PS NFM and TC, a PS-TC-SDBS supramolecular complex with a large rigid structure was formed, thus enhancing the fluorescence of TC (λ em = 550 nm). The dynamic solid-phase sensing mode is unique and can be employed to selectively adsorb TC, with reduced interference by impurities in complex samples, and thus the sensitive TC ratiometric fluorescent sensing with a detection limit of 2.14 nM, and fine visual colorimetric detection with polychromatic wide color range (red–orange-yellow-green) was realized. [ABSTRACT FROM AUTHOR]

The role of TANK-binding kinase 1 (TBK1) of humans and mice in innate immunity is well elucidated. Still, the molecular characterization and biological function of the TBK1 gene in herbivorous animals are less studied. Here, the open reading frame (ORF) of TBK1 of the cow and goat was firstly cloned and successfully expressed. The Phylogenetic tree analysis reveals that the TBK1 gene of goats and cows is similar to chicken and mute swans, respectively. Some evolutionary distances of the TBK1 gene were still present among different species. A slightly subcellular distribution difference was observed among full-length and truncated TBK1 of goats and cows. Dual-luciferase reporter assay has shown that the full-length TBK1 of goats and cows plays a vital role in the induction of IFN-β production. The viral infection experiment showed that the over-expression of the full-length TBK1 gene of the cow and goat significantly suppresses intracellular viral replication of the Lumpy skin disease virus (LSDV) in infected cells. Our study showed that TBK1 in the cows and goats is a crucial immunoregulatory for IFN-β production during viral infection, contributing to a better understanding of innate immunity in the herbivorous animal. • The TBK1 gene in cows and goats was first cloned and successfully expressed. • Phylogenetic tree and evolutionary analysis reveal that the TBK1 gene was still present among different species. • A slightly subcellular distribution difference was observed among full-length and truncated TBK1 of goats and cows. • The full-length TBK1 of the goats and cows plays a vital role in the induction of IFN-β and microbial infections. [ABSTRACT FROM AUTHOR]

Polydopamine polystyrene nanofibers mat (PDA-PS NFsM) was prepared as an adsorbent for the simultaneous removal of multiple antibiotic residues in environmental water for the first time. PDA-PS NFsM can directly be used to adsorb 18 antibiotic residues belonging to 8 classes without any pretreatment of water samples. The adsorption process was completed within 5 min. All antibiotics could be removed at the same time, and the removal rate of each target was above 85%. Moreover, the used PDA-PS NFsM can be easily separated from the environmental water by taking out directly, and can be reused for 10 times after simple regeneration. The thermodynamic and kinetic properties of PDA-PS NFsM adsorption of antibiotic residues were further investigated consequently. It was found that the adsorptions of PDA-PS NFsM to the targets were spontaneous and endothermic process (ΔG<0, ΔH>0, ΔS>0). The results of adsorption kinetic experiments illustrated that the adsorption process was rapid, the adsorption equilibrium of which can be reached in 5 min. Adsorption isotherm experiments proved that the adsorption process of PDA-PS NFsM was consistent with Langmuir adsorption (R2 > 0.994), and the maximum adsorption capacity of PDA-PS NFsM towards all targets were 123.76 mg g−1. The developed method is rapid and simple, and can efficiently adsorb and remove a variety of antibiotics in environmental water, which has good practical application prospect. [Display omitted] • PDA-PS NFsM is firstly prepared and evaluated as a novel absorbent for water treatment. • A simple method for simultaneous removal of multi-class antibiotic residues was developed. • Only 5 min were needed for adsorption equilibrium, and the removal rates were all higher than 85%. • Direct adsorption without any pretreatment of the water sample or any special conditions. • The used NFsM can be directly removed and reused for 10 times after simple regeneration. [ABSTRACT FROM AUTHOR]