28 results on '"Walder, Ken"'
Search Results
2. The role of metformin as a treatment for neuropsychiatric illness
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Dodd, Seetal, Sominsky, Luba, Siskind, Dan, Bortolasci, Chiara C, Carvalho, Andre F., Maes, Michael, Walker, Adam J., Walder, Ken, Yung, Alison R, Williams, Lana J., Myles, Hannah, Watson, Tayler, and Berk, Michael
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- 2022
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3. The putative role of oxidative stress and inflammation in the pathophysiology of sleep dysfunction across neuropsychiatric disorders: Focus on chronic fatigue syndrome, bipolar disorder and multiple sclerosis.
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Morris, Gerwyn, Stubbs, Brendon, Köhler, Cristiano A., Walder, Ken, Slyepchenko, Anastasiya, Berk, Michael, Carvalho, André F., Köhler, Cristiano A, and Carvalho, André F
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Sleep and circadian abnormalities are prevalent and burdensome manifestations of diverse neuro-immune diseases, and may aggravate the course of several neuropsychiatric disorders. The underlying pathophysiology of sleep abnormalities across neuropsychiatric disorders remains unclear, and may involve the inter-play of several clinical variables and mechanistic pathways. In this review, we propose a heuristic framework in which reciprocal interactions of immune, oxidative and nitrosative stress, and mitochondrial pathways may drive sleep abnormalities across potentially neuroprogressive disorders. Specifically, it is proposed that systemic inflammation may activate microglial cells and astrocytes in brain regions involved in sleep and circadian regulation. Activated glial cells may secrete pro-inflammatory cytokines (for example, interleukin-1 beta and tumour necrosis factor alpha), nitric oxide and gliotransmitters, which may influence the expression of key circadian regulators (e.g., the Circadian Locomotor Output Cycles Kaput (CLOCK) gene). Furthermore, sleep disruption may further aggravate oxidative and nitrosative, peripheral immune activation, and (neuro) inflammation across these disorders in a vicious pathophysiological loop. This review will focus on chronic fatigue syndrome, bipolar disorder, and multiple sclerosis as exemplars of neuro-immune disorders. We conclude that novel therapeutic targets exploring immune and oxidative & nitrosative pathways (p.e. melatonin and molecular hydrogen) hold promise in alleviating sleep and circadian dysfunction in these disorders. [ABSTRACT FROM AUTHOR]
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- 2018
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4. The role of hypernitrosylation in the pathogenesis and pathophysiology of neuroprogressive diseases.
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Morris, Gerwyn, Walder, Ken, Tye, Susannah J., Carvalho, André F., Berk, Michael, Maes, Michael, and Lucas, Kurt
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NITROSYLATION , *NEURODEGENERATION , *APOPTOSIS , *NEUROTROPHINS , *DEVELOPMENTAL neurobiology - Abstract
There is a wealth of data indicating that de novo protein S-nitrosylation in general and protein transnitrosylation in particular mediates the bulk of nitric oxide signalling. These processes enable redox sensing and facilitate homeostatic regulation of redox dependent protein signalling, function, stability and trafficking. Increased S-nitrosylation in an environment of increasing oxidative and nitrosative stress (O&NS) is initially a protective mechanism aimed at maintaining protein structure and function. When O&NS becomes severe, mechanisms governing denitrosylation and transnitrosylation break down leading to the pathological state referred to as hypernitrosylation (HN). Such a state has been implicated in the pathogenesis and pathophysiology of several neuropsychiatric and neurodegenerative diseases and we investigate its potential role in the development and maintenance of neuroprogressive disorders. In this paper, we propose a model whereby the hypernitrosylation of a range of functional proteins and enzymes lead to changes in activity which conspire to produce at least some of the core abnormalities contributing to the development and maintenance of pathology in these illnesses. [ABSTRACT FROM AUTHOR]
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- 2018
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5. A model of the mitochondrial basis of bipolar disorder.
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Morris, Gerwyn, Walder, Ken, McGee, Sean L., Dean, Olivia M., Tye, Susannah J., Maes, Michael, and Berk, Michael
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BIPOLAR disorder , *MITOCHONDRIAL physiology , *MENTAL depression , *OXIDATIVE phosphorylation , *BRAIN-derived neurotrophic factor , *ADENOSINE triphosphatase - Abstract
Background Bipolar disorder phenomenologically is a biphasic disorder of energy availability; increased in mania and decreased in depression. In consort, there is accumulating evidence indicating increased mitochondrial respiration and ATP production in bipolar mania which contrasts with decreased mitochondrial function in patients in the euthymic or depressive phase of the illness. Consequently, the central thesis of this paper is that bipolar disorder is due to a phasic dysregulation of mitochondrial biogenergetics. The elements responsible for this dysregulation may thus represent critical treatment targets for mood disorders, and are the subject of this paper. Discussion There are many potential mediators of mitochondrial function which collectively are implicated in bipolar disorder. Levels of oxidative stress, pro-inflammatory cytokines and intracellular calcium ions are all higher in bipolar mania than in the euthymic and depressive phases of the illness. Increased levels of calcium ions can partly account for increased oxidative phosphorylation via well documented pathways such as the modulation of the F 1 –F O elements of ATP synthase. Likewise, increased levels of oxidative stress and pro-inflammatory cytokines lead to the upregulation of AMPK, SIRT-1, SIRT-3 and NAD + which directly stimulate oxidative phosphorylation. Uric acid and melatonin are also differentially elevated in bipolar mania and both molecules stimulate the production of ATP. The pro-apoptotic, neurotoxic and mitotoxic effects of elevated glutamate, dopamine and GSK-3 in bipolar mania may be counterbalanced by higher basal levels and activity of p53, Bcl-2, PI3K and Akt in an environment of elevated uric acid and decreased BDNF. Summary Details of these pathways are discussed as an explanatory model for the existence of increased ATP generation in mania. We also offer a model explaining the biphasic nature of mitochondrial respiration in bipolar disorder and the transition between mania and depression based on increasing levels of TNFα, ROS, NO, AMPK and SIRT-1 together with the antagonistic relationship between p53 and NF-κB. [ABSTRACT FROM AUTHOR]
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- 2017
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6. Wireless Optogenetics: An Exploration of Portable Microdevices for Small Animal Photostimulation.
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Kale, Rajas P., Kouzani, Abbas Z., Berk, Michael, Walder, Ken, Berk, Julian, and Tye, Susannah J.
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Preclinical research in optogeneticneuromodulation in small laboratory animals allows far greater control of neural circuitry. This precision provides an enhanced opportunity for understanding the neural basis of behavior. However, behavioral neuroscience research is limited by conventional benchtop optogenetic systems. By necessity, the animal is tethered to the light source external to the testing environment. Portable optogeneticmicrodevices enhance the potential for valid behavioral testing in naturalistic conditions by eliminating tethering and enabling free and unrestricted movement. This paper reviews recent advances in the development of portable optogeneticmicrodevices supported by wireless power transfer. Light sources and fiber coupling are common problems in optogenetic systems and are addressed. Device designs and parameters are summarized, along with advances in component technology for energy storage and distribution that make these devices possible. [ABSTRACT FROM AUTHOR]
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- 2015
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7. Intertwined associations between oxidative and nitrosative stress and endocannabinoid system pathways: Relevance for neuropsychiatric disorders.
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Morris, Gerwyn, Walder, Ken, Berk, Michael, Carvalho, Andre F., Marx, Wolf, Bortolasci, Chiara C., Yung, Alison R., Puri, Basant K., and Maes, Michael
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OXIDATIVE stress , *NEUROBEHAVIORAL disorders , *CANNABINOID receptors , *PERIPHERAL nervous system , *B cells , *CENTRAL nervous system , *T cells , *DENDRITIC cells - Abstract
The endocannabinoid system (ECS) appears to regulate metabolic, cardiovascular, immune, gastrointestinal, lung, and reproductive system functions, as well as the central nervous system. There is also evidence that neuropsychiatric disorders are associated with ECS abnormalities as well as oxidative and nitrosative stress pathways. The goal of this mechanistic review is to investigate the mechanisms underlying the ECS's regulation of redox signalling, as well as the mechanisms by which activated oxidative and nitrosative stress pathways may impair ECS-mediated signalling. Cannabinoid receptor (CB)1 activation and upregulation of brain CB2 receptors reduce oxidative stress in the brain, resulting in less tissue damage and less neuroinflammation. Chronically high levels of oxidative stress may impair CB1 and CB2 receptor activity. CB1 activation in peripheral cells increases nitrosative stress and inducible nitric oxide (iNOS) activity, reducing mitochondrial activity. Upregulation of CB2 in the peripheral and central nervous systems may reduce iNOS, nitrosative stress, and neuroinflammation. Nitrosative stress may have an impact on CB1 and CB2-mediated signalling. Peripheral immune activation, which frequently occurs in response to nitro-oxidative stress, may result in increased expression of CB2 receptors on T and B lymphocytes, dendritic cells, and macrophages, reducing the production of inflammatory products and limiting the duration and intensity of the immune and oxidative stress response. In conclusion, high levels of oxidative and nitrosative stress may compromise or even abolish ECS-mediated redox pathway regulation. Future research in neuropsychiatric disorders like mood disorders and deficit schizophrenia should explore abnormalities in these intertwined signalling pathways. • Psychiatric disorders are associated with endocannabinoid system (ECS) alterations and oxidative and nitrosative stress (O&NS). • Cannabinoid receptor (CB)1 and CB2 reduce O&NS in the brain. • Activated O&NS pathways impair ECS-mediated signalling. • Chronic O&NS may impair CB1 and CB2 receptor activity. • High O&NS levels may compromise ECS-mediated redox pathway regulation. [ABSTRACT FROM AUTHOR]
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- 2022
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8. The characterization of Abelson helper integration site–1 in skeletal muscle and its links to the metabolic syndrome.
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Prior, Matthew J., Foletta, Victoria C., Jowett, Jeremy B., Segal, David H., Carless, Melanie A., Curran, Joanne E., Dyer, Tom D., Moses, Eric K., McAinch, Andrew J., Konstantopoulos, Nicky, Bozaoglu, Kiymet, Collier, Greg R., Cameron-Smith, David, Blangero, John, and Walder, Ken R.
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METABOLIC syndrome ,STRIATED muscle ,GENE expression ,TYPE 2 diabetes ,GENETIC polymorphisms ,HUMAN genetic variation ,DISEASE progression - Abstract
Abstract: The human Abelson helper integration site–1 (AHI1) gene is associated with both neurologic and hematologic disorders; however, it is also located in a chromosomal region linked to metabolic syndrome phenotypes and was identified as a type 2 diabetes mellitus susceptibility gene from a genomewide association study. To further define a possible role in type 2 diabetes mellitus development, AHI1 messenger RNA expression levels were investigated in a range of tissues and found to be highly expressed in skeletal muscle as well as displaying elevated levels in brain regions and gonad tissues. Further analysis in a rodent polygenic animal model of obesity and type 2 diabetes mellitus identified increased Ahi-1 messenger RNA levels in red gastrocnemius muscle from fasted impaired glucose–tolerant and diabetic rodents compared with healthy animals (P < .002). Moreover, elevated gene expression levels were confirmed in skeletal muscle from fasted obese and type 2 diabetes mellitus human subjects (P < .02). RNAi-mediated suppression of Ahi-1 resulted in increased glucose transport in rat L6 myotubes in both the basal and insulin-stimulated states (P < .01). Finally, single nucleotide polymorphism association studies identified 2 novel AHI1 genetic variants linked with fasting blood glucose levels in Mexican American subjects (P < .037). These findings indicate a novel role for AHI1 in skeletal muscle and identify additional genetic links with metabolic syndrome phenotypes suggesting an involvement of AHI1 in the maintenance of glucose homeostasis and type 2 diabetes mellitus progression. [Copyright &y& Elsevier]
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- 2010
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9. Regulation of Skeletal Muscle Oxidative Capacity and Insulin Signaling by the Mitochondrial Rhomboid Protease PARL.
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Civitarese, Anthony E., MacLean, Paul S., Carling, Stacy, Kerr-Bayles, Lyndal, McMillan, Ryan P., Pierce, Anson, Becker, Thomas C., Moro, Cedric, Finlayson, Jean, Lefort, Natalie, Newgard, Christopher B., Mandarino, Lawrence, Cefalu, William, Walder, Ken, Collier, Greg R., Hulver, Matthew W., Smith, Steven R., and Ravussin, Eric
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CELLULAR control mechanisms ,INSULIN resistance ,CELLULAR signal transduction ,MITOCHONDRIAL pathology ,GENETICS of type 2 diabetes ,TISSUE remodeling ,STRIATED muscle - Abstract
Summary: Type 2 diabetes mellitus (T2DM) and aging are characterized by insulin resistance and impaired mitochondrial energetics. In lower organisms, remodeling by the protease pcp1 (PARL ortholog) maintains the function and lifecycle of mitochondria. We examined whether variation in PARL protein content is associated with mitochondrial abnormalities and insulin resistance. PARL mRNA and mitochondrial mass were both reduced in elderly subjects and in subjects with T2DM. Muscle knockdown of PARL in mice resulted in malformed mitochondrial cristae, lower mitochondrial content, decreased PGC1α protein levels, and impaired insulin signaling. Suppression of PARL protein in healthy myotubes lowered mitochondrial mass and insulin-stimulated glycogen synthesis and increased reactive oxygen species production. We propose that lower PARL expression may contribute to the mitochondrial abnormalities seen in aging and T2DM. [Copyright &y& Elsevier]
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- 2010
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10. The lipid paradox in neuroprogressive disorders: Causes and consequences.
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Morris, Gerwyn, Berk, Michael, Walder, Ken, O'Neil, Adrienne, Maes, Michael, and Puri, Basant K.
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CARDIOVASCULAR diseases , *LDL cholesterol , *DRUG target , *LIPIDS , *PATHOLOGICAL physiology - Abstract
• Chronic systemic inflammation is associated with increased cardiovascular disease. • It is also associated with the pathophysiology of neuroprogressive disorders. • Both can occur with low LDL and low cholesterol: a lipid paradox. • Inflammatory molecules released by activated neutrophils play a crucial role. • The associated molecular mechanisms suggest potential therapeutic targets. Chronic systemic inflammation is associated with an increased risk of cardiovascular disease in an environment of low low-density lipoprotein (LDL) and low total cholesterol and with the pathophysiology of neuroprogressive disorders. The causes and consequences of this lipid paradox are explored. Circulating activated neutrophils can release inflammatory molecules such as myeloperoxidase and the pro-inflammatory cytokines interleukin-1 beta, interleukin-6 and tumour necrosis factor-alpha. Since activated neutrophils are associated with atherosclerosis and cardiovascular disease and with major depressive disorder, bipolar disorder and schizophrenia, it seems reasonable to hypothesise that the inflammatory molecules released by them may act as mediators of the link between systemic inflammation and the development of atherosclerosis in neuroprogressive disorders. This hypothesis is tested by considering the association at a molecular level of systemic inflammation with increased LDL oxidation; increased small dense LDL levels; increased lipoprotein (a) concentration; secretory phospholipase A 2 activation; cytosolic phospholipase A 2 activation; increased platelet activation; decreased apolipoprotein A1 levels and function; decreased paroxonase-1 activity; hyperhomocysteinaemia; and metabolic endotoxaemia. These molecular mechanisms suggest potential therapeutic targets. [ABSTRACT FROM AUTHOR]
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- 2021
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11. Recombinant Balsamin induces apoptosis in liver and breast cancer cells via cell cycle arrest and regulation of apoptotic pathways.
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Ajji, Parminder K., Binder, Marley J., Walder, Ken, and Puri, Munish
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CELL cycle regulation , *LIVER cancer , *CANCER cells , *BREAST cancer , *RIBOSOMES , *CELL cycle - Abstract
• rBalsamin (a ribosome inactivating protein) possesses for anti-cancer activity. • rBalsamin inhibited cell viability of liver and breast cancer cells. • rBalsamin induced apoptosis in liver and breast cancer cells via death receptor and mitochondrial associated apoptotic pathways. • rBalsamin induces cell cycle arrest in liver and breast cancer cells. Recombinant balsamin (rBalsamin), a type I ribosome inactivating protein classified as RNA N-glycosidase, is known to possess antibacterial and DNase like activity. However, its anticancer properties have not yet been examined. In this study, we aimed to investigate the potential cytotoxicity of rBalsamin on hepatocellular (HepG2 and H4IIE) and breast (MCF-7 and BT549) carcinoma cells and the related mechanism. rBalsamin arrested cell cycle at G or S phase and increased the level of caspase-3/8. The expression of Bax, Bid, Bad and p53 increased and that of Bcl-2 and BCL-xl decreased in liver and breast cancer cells with rBalsamin treatment. We found that rBalsamin inhibited cell viability of liver and breast cancer cells in a concentration and time dependent manner with IC 50 ranging between 18.92 to > 200 μg/mL. These findings suggest that rBalsamin induced apoptosis in liver and breast cancer cells via death receptor and mitochondrial associated apoptotic pathways, could prove beneficial in the field of cancer therapeutics, highlighting its potential as a functional food ingredient. [ABSTRACT FROM AUTHOR]
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- 2020
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12. Purification and functional characterization of recombinant balsamin, a ribosome-inactivating protein from Momordica balsamina.
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Ajji, Parminder K., Sonkar, Shailendra P., Walder, Ken, and Puri, Munish
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MOMORDICA , *GENE expression , *ESCHERICHIA coli , *PLASMIDS , *FUNCTIONAL analysis - Abstract
Balsamin, a type I ribosome-inactivating protein (RIP), has been shown to inhibit HIV-1 replication at the translation step. Our recent studies have shown that balsamin also possess anti-tumor, antibacterial and DNase-like activity, however, the amount of natural balsamin in Momordica balsamina seeds is limited and preclinical studies require large quantities of pure, bioactive balsamin. Therefore, in this study, we cloned the balsamin gene, expressed it in E. coli BL21 (DE3) strain and purified it by nickel affinity chromatography. Functional analysis indicated that balsamin exhibits both RNA N -glycosidase activity, releasing the Endo-fragment from rabbit reticulocyte rRNA, and DNase-like activity, converting the supercoiled form of a plasmid into the linear form in a concentration-dependent manner. Analysis of secondary structure revealed that recombinant balsamin mainly consisted of α-helical and random coiled with minimal turns and β-sheets. Recombinant balsamin was found to be stable in the temperature range of 20–60 °C and pH range of 6–9. Antimicrobial assays showed that the minimum inhibitory concentrations of recombinant balsamin for various pathogens ranged between 1.56 and 12.5 μg/ml. Heterologous expression and purification of balsamin carries great importance as it provides an alternative approach for large-scale preparation of biologically active recombinant balsamin, which is difficult from its natural source. [ABSTRACT FROM AUTHOR]
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- 2018
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13. Common effects of bipolar disorder medications on expression quantitative trait loci genes.
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Truong, Trang TT., Bortolasci, Chiara C., Kidnapillai, Srisaiyini, Spolding, Briana, Panizzutti, Bruna, Liu, Zoe SJ., Watmuff, Brad, Kim, Jee Hyun, Dean, Olivia M., Richardson, Mark, Berk, Michael, and Walder, Ken
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LOCUS (Genetics) , *BIPOLAR disorder , *GENE expression , *GENE expression profiling , *DRUGS , *GENETIC regulation - Abstract
The molecular mechanism(s) underpinning the clinical efficacy of the current drugs for bipolar disorder (BD) are largely unknown. This study evaluated the transcriptional perturbations potentially playing roles in the therapeutic efficacy of four commonly prescribed psychotropic drugs used to treat BD. NT2-N cells were treated with lamotrigine, lithium, quetiapine, valproate or vehicle control for 24 h. Genome-wide mRNA expression was quantified by RNA-sequencing. Incorporating drug-induced gene expression profiles with BD-associated transcriptional changes from post-mortem brains, we identified potential therapeutic-relevant genes associated with both drug treatments and BD pathophysiology and focused on expression quantitative trait loci (eQTL) genes with genome-wide association with BD. Each eQTL gene was ranked based on its potential role in the therapeutic effect across multiple drugs. The expression of highest-ranked eQTL genes were measured by RT-qPCR to confirm their transcriptional changes observed in RNA-seq. We found 775 genes for which at least 2 drugs reversed expression levels relative to the differential expression in post-mortem brains. Pathway analysis identified enriched biological processes highlighting mitochondrial and endoplasmic reticulum function. Differential expression of SRPK2 and CHDH was confirmed by RT-qPCR following multiple-dose treatments. We pinpointed potential genes involved in the beneficial effects of drugs used for BD and their main associated biological pathways. CHDH , which encodes a mitochondrial protein, had a significant dose-responsive downregulation following treatment with increasing doses of quetiapine and lamotrigine, which in combination with the enriched mitochondrial pathways suggests potential therapeutic roles and demand more studies on mitochondrial involvement in BD to identify novel treatment targets. [ABSTRACT FROM AUTHOR]
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- 2022
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14. Baseline serum amino acid levels predict treatment response to augmentation with N-acetylcysteine (NAC) in a bipolar disorder randomised trial.
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Bortolasci, Chiara C., Turner, Alyna, Mohebbi, Mohammadreza, Liu, Zoe SJ., Ashton, Melanie, Gray, Laura, Marx, Wolfgang, Walker, Adam J., Kowalski, Greg M., Jacka, Felice, Berk, Michael, Dean, Olivia M., and Walder, Ken
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AMINO acids , *BIPOLAR disorder , *GLUTAMIC acid , *PHENYLALANINE , *ACETYLCYSTEINE - Abstract
N-acetylcysteine (NAC) acts on glutamatergic and redox systems, two systems implicated in the pathophysiology of bipolar disorder (BD). This has led to the investigation of NAC as a potential candidate for the treatment of BD. The aim of this study was to investigate metabolomic markers to identify predictors of NAC response in a cohort of BD participants. This study is a secondary analysis of a 16-week, multi-site, randomized, double-blinded, parallel-group, placebo-controlled trial in BD participants with a current acute depressive episode. This study included trial participants who received either NAC 2000 mg/day, or placebo. Participants (NAC: n = 31, placebo: n = 29) were assessed at baseline and week 16 using the Montgomery Åsberg Depression Rating Scale (MADRS) and were dichotomised into "responders" (MADRS at week 16 < 50% of MADRS at baseline) and "non-responders" (MADRS at week 16 > 50% at baseline). Untargeted gas chromatography–mass spectrometry analysis was performed to analyse baseline levels of 68 serum metabolites. Of the nine metabolites that differentiated placebo and NAC groups, five were amino acids with lower levels in the NAC responder group compared with the NAC non-responders. Further analysis generated a predictive model of MADRS improvement including glycine, norleucine, threonine, proline, phenylalanine, tyrosine, glutamic acid, lysine and leucine (R2 = 0.853; adjusted R2 = 0.733). This prediction model predicted 85% of the variance in MADRS outcome after adjunctive treatment with NAC. BD participants with lower serum levels of free amino acids at baseline may be more likely to respond to adjunctive treatment with NAC. [ABSTRACT FROM AUTHOR]
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- 2021
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15. Polyphenols as adjunctive treatments in psychiatric and neurodegenerative disorders: Efficacy, mechanisms of action, and factors influencing inter-individual response.
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Morris, Gerwyn, Gamage, Elizabeth, Travica, Nikolaj, Berk, Michael, Jacka, Felice N., O'Neil, Adrienne, Puri, Basant K., Carvalho, Andre F., Bortolasci, Chiara C., Walder, Ken, and Marx, Wolfgang
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NEURODEGENERATION , *PSYCHIATRIC treatment , *GUT microbiome , *MENTAL illness , *POLYPHENOLS , *TREATMENT effectiveness - Abstract
The pathophysiology of psychiatric and neurodegenerative disorders is complex and multifactorial. Polyphenols possess a range of potentially beneficial mechanisms of action that relate to the implicated pathways in psychiatric and neurodegenerative disorders. The aim of this review is to highlight the emerging clinical trial and preclinical efficacy data regarding the role of polyphenols in mental and brain health, elucidate novel mechanisms of action including the gut microbiome and gene expression, and discuss the factors that may be responsible for the mixed clinical results; namely, the role of interindividual differences in treatment response and the potentially pro-oxidant effects of some polyphenols. Further clarification as part of larger, well conducted randomized controlled trials that incorporate precision medicine methods are required to inform clinical efficacy and optimal dosing regimens. [Display omitted] • Polyphenols may be beneficial to psychiatric and neurodegenerative disorders. • Potential mechanisms of action include modulating gut microbiome and gene expression. • Differences in metabolism and pro-oxidant properties may affect clinical outcomes. • Further clinical data are required to establish efficacy and optimal dosing regimens. [ABSTRACT FROM AUTHOR]
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- 2021
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16. The role of high-density lipoprotein cholesterol, apolipoprotein A and paraoxonase-1 in the pathophysiology of neuroprogressive disorders.
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Morris, Gerwyn, Puri, Basant K., Bortolasci, Chiara C., Carvalho, Andre, Berk, Michael, Walder, Ken, Moreira, Estefania G., and Maes, Michael
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APOLIPOPROTEIN A , *HIGH density lipoproteins , *MEDITERRANEAN diet , *KETOGENIC diet , *POMEGRANATE juice - Abstract
• HDL, ApoA1 and PON1 are related to inflammation and lipid peroxidation in psychiatry. • Reduced HDL contributes both localised and systemic inflammation. • Homocysteine contributes to inflammation, oxidative stress and mitochondrial dysfunction. Lowered high-density lipoprotein (HDL) cholesterol has been reported in major depressive disorder, bipolar disorder, first episode of psychosis, and schizophrenia. HDL, its major apolipoprotein component, ApoA1, and the antioxidant enzyme paraoxonase (PON)1 (which is normally bound to ApoA1) all have anti-atherogenic, antioxidant, anti-inflammatory, and immunomodulatory roles, which are discussed in this paper. The paper details the pathways mediating the anti-inflammatory effects of HDL, ApoA1 and PON1 and describes the mechanisms leading to compromised HDL and PON1 levels and function in an environment of chronic inflammation. The molecular mechanisms by which changes in HDL, ApoA1 and PON1 might contribute to the pathophysiology of the neuroprogressive disorders are explained. Moreover, the anti-inflammatory actions of ApoM-mediated sphingosine 1-phosphate (S1P) signalling are reviewed as well as the deleterious effects of chronic inflammation and oxidative stress on ApoM/S1P signalling. Finally, therapeutic interventions specifically aimed at improving the levels and function of HDL and PON1 while reducing levels of inflammation and oxidative stress are considered. These include the so-called Mediterranean diet, extra virgin olive oil, polyphenols, flavonoids, isoflavones, pomegranate juice, melatonin and the Mediterranean diet combined with the ketogenic diet. [ABSTRACT FROM AUTHOR]
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- 2021
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17. Can endolysosomal deacidification and inhibition of autophagy prevent severe COVID-19?
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Morris, Gerwyn, Athan, Eugene, Walder, Ken, Bortolasci, Chiara C., O'Neil, Adrienne, Marx, Wolf, Berk, Michael, Carvalho, André F., Maes, Michael, and Puri, Basant K.
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COVID-19 , *AUTOPHAGY , *SECRETORY granules , *NF-kappa B , *SARS-CoV-2 , *INFLAMMATORY mediators , *AUTOANTIBODIES - Abstract
The possibility is examined that immunomodulatory pharmacotherapy may be clinically useful in managing the pandemic coronavirus disease 2019 (COVID-19), known to result from infection by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), a positive-sense single-stranded RNA virus. The dominant route of cell entry of the coronavirus is via phagocytosis, with ensconcement in endosomes thereafter proceeding via the endosomal pathway, involving transfer from early (EEs) to late endosomes (LEs) and ultimately into lysosomes via endolysosomal fusion. EE to LE transportation is a rate-limiting step for coronaviruses. Hence inhibition or dysregulation of endosomal trafficking could potentially inhibit SARS-CoV-2 replication. Furthermore, the acidic luminal pH of the endolysosomal system is critical for the activity of numerous pH-sensitive hydrolytic enzymes. Golgi sub-compartments and Golgi-derived secretory vesicles also depend on being mildly acidic for optimal function and structure. Activation of endosomal toll-like receptors by viral RNA can upregulate inflammatory mediators and contribute to a systemic inflammatory cytokine storm, associated with a worsened clinical outcome in COVID-19. Such endosomal toll-like receptors could be inhibited by the use of pharmacological agents which increase endosomal pH, thereby reducing the activity of acid-dependent endosomal proteases required for their activity and/or assembly, leading to suppression of antigen-presenting cell activity, decreased autoantibody secretion, decreased nuclear factor-kappa B activity and decreased pro-inflammatory cytokine production. It is also noteworthy that SARS-CoV-2 inhibits autophagy, predisposing infected cells to apoptosis. It is therefore also suggested that further pharmacological inhibition of autophagy might encourage the apoptotic clearance of SARS-CoV-2-infected cells. Unlabelled Image [ABSTRACT FROM AUTHOR]
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- 2020
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18. Shared pathways for neuroprogression and somatoprogression in neuropsychiatric disorders.
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Morris, Gerwyn, Puri, Basant K., Walker, Adam J., Maes, Michael, Carvalho, Andre F., Bortolasci, Chiara C., Walder, Ken, and Berk, Michael
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NEUROBEHAVIORAL disorders , *PATHOLOGY , *MENTAL illness , *METABOLIC syndrome , *CARDIOVASCULAR diseases , *ACTIVATED protein C resistance - Abstract
• Neuropsychiatric diseases predispose individuals to obesity and vice versa. • Pathophysiological processes are similar in obesity and neuropsychiatric disease. • Processes observed in neuropsychiatric disease contribute to developing obesity. • Processes observed in obesity contribute to developing neuropsychiatric disease. • Adjunctive agents can target these processes, with possibly ameliorative effects. Activated immune-inflammatory, oxidative and nitrosative stress (IO&NS) pathways and consequent mitochondrial aberrations are involved in the pathophysiology of psychiatric disorders including major depression, bipolar disorder and schizophrenia. They offer independent and shared contributions to pathways underpinning medical comorbidities including insulin resistance, metabolic syndrome, obesity and cardiovascular disease - herein conceptualized as somatoprogression. This narrative review of human studies aims to summarize relationships between IO&NS pathways, neuroprogression and somatoprogression. Activated IO&NS pathways, implicated in the neuroprogression of psychiatric disorders, affect the pathogenesis of comorbidities including insulin resistance, dyslipidaemia, obesity and hypertension, and by inference, metabolic syndrome. These conditions activate IO&NS pathways, exacerbating neuroprogression in psychiatric disorders. The processes whereby proinflammatory cytokines, nitrosative and endoplasmic reticulum stress, NADPH oxidase isoforms, PPARγ inactivation, SIRT1 deficiency and intracellular signalling pathways impact lipid metabolism and storage are considered. Through associations between body mass index, chronic neuroinflammation and FTO expression, activation of IO&NS pathways arising from somatoprogression may contribute to neuroprogression. Early evidence highlights the potential of adjuvants targeting IO&NS pathways for treating somatoprogression and neuroprogression. [ABSTRACT FROM AUTHOR]
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- 2019
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19. Bioenergetics and synaptic plasticity as potential targets for individualizing treatment for depression.
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Price, J. Blair, Bronars, Carrie, Erhardt, Sophie, Cullen, Kathyrn R., Schwieler, Lilly, Berk, Michael, Walder, Ken, Mcgee, Sean L., Frye, Mark A., and Tye, Susannah J.
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MENTAL depression , *THERAPEUTICS , *BIOENERGETICS , *KYNURENINE , *BIPOLAR disorder , *NEUROSCIENCES - Abstract
Disruptions of bioenergetic signaling and neurogenesis are hallmarks of depression physiology and are often the product of dysregulation of the inflammatory, stress-response, and metabolic systems. These systems are extensively interrelated at the physiological level, yet the bulk of the literature to date addresses pathophysiological mechanisms in isolation. A more integrated understanding of the etiology, progression, and treatment response profiles of depression is possible through wider consideration of relevant preclinical and clinical studies that examine the result of disruptions in these systems. Here, we review recent data demonstrating the critical effects of bioenergetic disruption on neuroplasticity and the development and progression of depressive illness. We further highlight the interactive and dynamic nature of the inflammatory and stress response systems and how disruption of these systems influences bioenergetic signaling pathways critical to treatment outcomes. In so doing, we underscore the pressing need to reconsider the implications of treatment resistance and present a framework for developing novel, personalized treatment approaches for depression. [ABSTRACT FROM AUTHOR]
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- 2018
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20. Depression and bone loss as risk factors for cognitive decline: A systematic review and meta-analysis.
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Mehta, Kanika, Thandavan, Sathya Priya, Mohebbi, Mohammadreza, Pasco, Julie A., Williams, Lana J., Walder, Ken, Ng, Boon Lung, and Gupta, Veer Bala
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COGNITION disorders , *MENTAL depression - Published
- 2022
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21. Chronic adrenocorticotrophic hormone treatment alters tricyclic antidepressant efficacy and prefrontal monoamine tissue levels
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Walker, Adam J., Burnett, Sandy A., Hasebe, Kyoko, McGillivray, Jane A., Gray, Laura J., McGee, Sean L., Walder, Ken, Berk, Michael, and Tye, Susannah J.
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ADRENOCORTICOTROPIC hormone , *ANTIDEPRESSANTS , *PHARMACODYNAMICS , *PREFRONTAL cortex , *MONOAMINE oxidase , *TISSUES , *HYPOTHALAMIC-pituitary-adrenal axis - Abstract
Abstract: Several animal models are currently utilised in the investigation of major depressive disorder; however, each is validated by its response to antidepressant pharmacotherapy. Few animal models consider the notion of antidepressant treatment resistance. Chronic daily administration of adrenocorticotropic hormone (ACTH) or corticosterone can alter behavioural responses to antidepressants, effectively blocking antidepressant efficacy. Herein, we demonstrate that ACTH-(1-24) (100μg/day; 14 days) blocks the immobility-reducing ‘antidepressant’ effects of a single dose of imipramine (10mg/kg) in the forced swim test. This finding was accompanied by altered monoamine tissue levels in the prefrontal cortex (PFC) 1h after exposure to the acute stress of the forced swim test. PFC tissue from ACTH pre-treated animals contained significantly higher serotonin, noradrenaline and adrenaline concentrations relative to saline pre-treated controls. Conversely, dopamine levels were significantly decreased. Altered plasma corticosterone responses to ACTH injections were observed over the treatment course. Measures were taken on treatment days 1, 4, 8, 11, 14 and 15. ACTH administration initially enhanced plasma corticosterone levels, however, these normalised to levels consistent with control animals by day 14. No differences in corticosterone levels were observed across the treatment time course in saline-treated animals. Taken together these results indicate that pre-treatment with ACTH (100μg/day; 14 days) blocks the antidepressant effects of imipramine (10mg/kg), significantly alters key PFC monoamine responses to stress and downregulates glucocorticoid responses. These results suggest that chronic ACTH treatment is a promising paradigm for elucidation of mechanisms mediating antidepressant treatment resistance. [Copyright &y& Elsevier]
- Published
- 2013
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22. Effects of rosiglitazone on intramyocellular lipid accumulation in Psammomys obesus
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Molero, Juan Carlos, Lee, Scott, Leizerman, Ilit, Chajut, Ayelet, Cooper, Adrian, and Walder, Ken
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ROSIGLITAZONE , *LIPIDS , *BIOACCUMULATION , *ELECTRON microscopy , *PSAMMOMYS obesus , *ANIMAL models of diabetes , *INSULIN resistance , *OBESITY - Abstract
Abstract: Objective: To examine the effects of rosiglitazone in intramyocellular lipid (IMCL) content in diabetic Psammomys obesus using novel electron microscopy technologies. Background: P. obesus is an unique polygenic model of obesity and type 2 diabetes. Male diabetic P. obesus were treated daily with 5 mg/Kg Rosiglitazone by oral gavage for 14 days. Data were compared with a group of age-matched diabetic P. obesus treated with saline vehicle. Methods: Assessment of insulin resistance and adiposity were determine before and after the treatment period by oral glucose tolerance test (oGTT) and dual energy X-ray absorptiometry (DEXA) analysis. We used a new scanning electron microscopy technology, (WETSEM) to investigate the effects of rosiglitazone administration on IMCL content, size and distribution in red gastrocnemius muscle. Results: Rosiglitazone treatment improved glucose tolerance in P. obesus with no difference in the overall body fat content although a significant reduction in subscapular fat mass was observed. Rosiglitazone changed the distribution of lipid droplet size in skeletal muscle. Treated animals tended to have smaller lipid droplets compared with saline-treated controls. Conclusions: Since smaller IMCL droplets are associated with improvements in insulin sensitivity, we propose that this may be an important mechanism by which rosiglitazone affects glucose tolerance. [Copyright &y& Elsevier]
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- 2010
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23. Identification of hypothalamic genes implicated in the development of obesity in Psammomys obesus using differential display PCR
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Trevaskis, James, McMillan, Janine S., Windmill, Kelly, Walder, Ken, and Collier, Greg R.
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HYPOTHALAMUS , *HOMEOSTASIS , *NEUROPEPTIDES , *GENES , *PEOPLE with diabetes - Abstract
The hypothalamus is a key central controller of energy homeostasis and is the source and/or site of action of many neuropeptides involved in this process. The aim of this study was to isolate hypothalamic genes differentially expressed between lean and obese Psammomys obesus, a polygenic animal model of obesity and type 2 diabetes. Differential display PCR was used to compare hypothalamic gene expression profiles of lean and healthy, obese and hyperinsulinemic, and obese, diabetic P. obesus in both the fed and fasted states. We conducted differential display with 180 separate primer combinations to amplify approximately 9000 expressed transcripts. Sixty differentially expressed bands were excised. Taqman PCR was performed on 36 of these transcripts to confirm differential gene expression in a larger sample population. Of these 36 transcripts, 9 showed homology to known genes, and 27 were considered to be novel sequences. Gene expression profiles for two of these genes are presented here. In conclusion, differential display PCR was successfully used to isolate several transcripts that may be involved in the central regulation of energy balance. We are currently conducting numerous studies to further investigate the role of these genes in the development of obesity in P. obesus. [Copyright &y& Elsevier]
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- 2004
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24. The endocannabinoid system and retinoic acid signaling combine to influence bone growth.
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Fraher, Daniel, Mann, Robert J., Dubuisson, Matthew J., Ellis, Megan K., Yu, Tingsheng, Walder, Ken, Ward, Alister C., Winkler, Christoph, and Gibert, Yann
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BONE growth , *TRETINOIN , *BONE resorption , *ORYZIAS latipes , *BRACHYDANIO , *POPULATION aging , *OSTEOCLASTOGENESIS - Abstract
Osteoporosis is an increasing burden on public health as the world-wide population ages and effective therapeutics are severely needed. Two pathways with high potential for osteoporosis treatment are the retinoic acid (RA) and endocannabinoid system (ECS) signaling pathways. We sought to elucidate the roles that these pathways play in bone development and maturation. Here, we use chemical treatments to modulate the RA and ECS pathways at distinct early, intermediate, and late times bone development in zebrafish. We further assessed osteoclast activity later in zebrafish and medaka. Finally, by combining sub-optimal doses of AR and ECS modulators, we show that enhancing RA signaling or reducing the ECS promote bone formation and decrease osteoclast abundance and activity. These data demonstrate that RA signaling and the ECS can be combined as sub-optimal doses to influence bone growth and may be key targets for potential therapeutics. [ABSTRACT FROM AUTHOR]
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- 2021
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25. Systemic inflammation and grey matter volume in schizophrenia and bipolar disorder: Moderation by childhood trauma severity.
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Quidé, Yann, Bortolasci, Chiara C., Spolding, Briana, Kidnapillai, Srisaiyini, Watkeys, Oliver J., Cohen-Woods, Sarah, Carr, Vaughan J., Berk, Michael, Walder, Ken, and Green, Melissa J.
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TUMOR necrosis factors , *BIPOLAR disorder , *INTERLEUKIN-6 , *C-reactive protein , *INDEPENDENT component analysis , *ENCEPHALITIS - Abstract
Elevated levels of systemic inflammation are consistently reported in both schizophrenia (SZ) and bipolar-I disorder (BD), and are associated with childhood trauma exposure. We tested whether childhood trauma exposure moderates associations between systemic inflammation and brain morphology in people with these diagnoses. Participants were 55 SZ cases, 52 BD cases and 59 healthy controls (HC) who underwent magnetic resonance imaging. Systemic inflammation was measured using a composite z-score derived from serum concentrations of interleukin 6, tumor necrosis factor alpha and C-reactive protein. Indices of grey matter volume covariation (GMC) were derived from independent component analysis. Childhood trauma was measured using the Childhood Trauma Questionnaire (CTQ Total score). A series of moderated moderation analyses indicated that increased systemic inflammation were associated with increased GMC in the striatum and cerebellum among all participants. Severity of childhood trauma exposure moderated the relationship between systemic inflammation and GMC in one component, differently among the groups. Specifically, decreased GMC in the PCC/precuneus, parietal lobule and postcentral gyrus, and increased GMC in the left middle temporal gyrus was associated with increased systemic inflammation in HC individuals exposed to high (but not low or average) levels of trauma and in SZ cases exposed to low (but not average or high) levels of trauma, but not in BD cases. Increased systemic inflammation is associated with grey matter changes in people with psychosis, and these relationships may be partially and differentially moderated by childhood trauma exposure according to diagnosis. • Increased inflammation is associated with increased striatal grey matter volume covariation (GMC) independently of diagnosis • Childhood trauma differently moderates the relationship between inflammation and brain morphology according to diagnosis • Increased inflammation is associated with decreased GMC in social brain regions in HCs exposed to high levels of trauma • Increased inflammation is associated with decreased GMC in these regions in SZ cases exposed to low levels of trauma • Childhood trauma did not moderate the relationship between inflammation and brain morphology in bipolar disorder cases. [ABSTRACT FROM AUTHOR]
- Published
- 2021
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26. Preventing the development of severe COVID-19 by modifying immunothrombosis.
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Morris, Gerwyn, Bortolasci, Chiara C., Puri, Basant K., Olive, Lisa, Marx, Wolfgang, O'Neil, Adrienne, Athan, Eugene, Carvalho, Andre, Maes, Michael, Walder, Ken, and Berk, Michael
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COVID-19 , *FIBRIN , *VASCULAR endothelial cells , *ADULT respiratory distress syndrome , *EPITHELIAL cells , *ALVEOLAR macrophages , *NEUTROPHILS - Abstract
COVID-19-associated acute respiratory distress syndrome (ARDS) is associated with significant morbidity and high levels of mortality. This paper describes the processes involved in the pathophysiology of COVID-19 from the initial infection and subsequent destruction of type II alveolar epithelial cells by SARS-CoV-2 and culminating in the development of ARDS. The activation of alveolar cells and alveolar macrophages leads to the release of large quantities of proinflammatory cytokines and chemokines and their translocation into the pulmonary vasculature. The presence of these inflammatory mediators in the vascular compartment leads to the activation of vascular endothelial cells platelets and neutrophils and the subsequent formation of platelet neutrophil complexes. These complexes in concert with activated endothelial cells interact to create a state of immunothrombosis. The consequence of immunothrombosis include hypercoagulation, accelerating inflammation, fibrin deposition, migration of neutrophil extracellular traps (NETs) producing neutrophils into the alveolar apace, activation of the NLRP3 inflammazome, increased alveolar macrophage destruction and massive tissue damage by pyroptosis and necroptosis Therapeutic combinations aimed at ameliorating immunothrombosis and preventing the development of severe COVID-19 are discussed in detail. Unlabelled Image [ABSTRACT FROM AUTHOR]
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- 2021
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27. The pathophysiology of SARS-CoV-2: A suggested model and therapeutic approach.
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Morris, Gerwyn, Bortolasci, Chiara C., Puri, Basant K., Olive, Lisa, Marx, Wolfgang, O'Neil, Adrienne, Athan, Eugene, Carvalho, Andre F., Maes, Michael, Walder, Ken, and Berk, Michael
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SARS-CoV-2 , *REACTIVE oxygen species , *VASCULAR endothelial cells , *EPITHELIAL cells , *CYTOKINE release syndrome , *NEUTROPHILS - Abstract
In this paper, a model is proposed of the pathophysiological processes of COVID-19 starting from the infection of human type II alveolar epithelial cells (pneumocytes) by SARS-CoV-2 and culminating in the development of ARDS. The innate immune response to infection of type II alveolar epithelial cells leads both to their death by apoptosis and pyroptosis and to alveolar macrophage activation. Activated macrophages secrete proinflammatory cytokines and chemokines and tend to polarise into the inflammatory M1 phenotype. These changes are associated with activation of vascular endothelial cells and thence the recruitment of highly toxic neutrophils and inflammatory activated platelets into the alveolar space. Activated vascular endothelial cells become a source of proinflammatory cytokines and reactive oxygen species (ROS) and contribute to the development of coagulopathy, systemic sepsis, a cytokine storm and ARDS. Pulmonary activated platelets are also an important source of proinflammatory cytokines and ROS, as well as exacerbating pulmonary neutrophil-mediated inflammatory responses and contributing to systemic sepsis by binding to neutrophils to form platelet-neutrophil complexes (PNCs). PNC formation increases neutrophil recruitment, activation priming and extraversion of these immune cells into inflamed pulmonary tissue, thereby contributing to ARDS. Sequestered PNCs cause the development of a procoagulant and proinflammatory environment. The contribution to ARDS of increased extracellular histone levels, circulating mitochondrial DNA, the chromatin protein HMGB1, decreased neutrophil apoptosis, impaired macrophage efferocytosis, the cytokine storm, the toll-like receptor radical cycle, pyroptosis, necroinflammation, lymphopenia and a high Th17 to regulatory T lymphocyte ratio are detailed. Unlabelled Image [ABSTRACT FROM AUTHOR]
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- 2020
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28. The compensatory antioxidant response system with a focus on neuroprogressive disorders.
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Morris, Gerwyn, Puri, Basant K., Walker, Adam J., Berk, Michael, Walder, Ken, Bortolasci, Chiara C., Marx, Wolfgang, Carvalho, Andre F., and Maes, Michael
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REACTIVE nitrogen species , *MITOGEN-activated protein kinases , *NUCLEAR receptors (Biochemistry) , *TALL-1 (Protein) , *OXIDOREDUCTASES , *SULFHYDRYL group , *NITRIC oxide , *OXIDATIVE stress - Abstract
Major antioxidant responses to increased levels of inflammatory, oxidative and nitrosative stress (ONS) are detailed. In response to increasing levels of nitric oxide, S-nitrosylation of cysteine thiol groups leads to post-transcriptional modification of many cellular proteins and thereby regulates their activity and allows cellular adaptation to increased levels of ONS. S-nitrosylation inhibits the function of nuclear factor kappa-light-chain-enhancer of activated B cells, toll-like receptor-mediated signalling and the activity of several mitogen-activated protein kinases, while activating nuclear translocation of nuclear factor (erythroid-derived 2)-like 2 (Nrf2 or NFE2L2); in turn, the redox-regulated activation of Nrf2 leads to increased levels and/or activity of key enzymes and transporter systems involved in the glutathione system. The Nrf2/Kelch-like ECH-associated protein-1 axis is associated with upregulation of NAD(P)H:quinone oxidoreductase 1, which in turn has anti-inflammatory effects. Increased Nrf2 transcriptional activity also leads to activation of haem oxygenase-1, which is associated with upregulation of bilirubin, biliverdin and biliverdin reductase as well as increased carbon monoxide signalling, anti-inflammatory and antioxidant activity. Associated transcriptional responses, which may be mediated by retrograde signalling owing to elevated hydrogen peroxide, include the unfolded protein response (UPR), mitohormesis and the mitochondrial UPR; the UPR also results from increasing levels of mitochondrial and cytosolic reactive oxygen species and reactive nitrogen species leading to nitrosylation, glutathionylation, oxidation and nitration of crucial cysteine and tyrosine causing protein misfolding and the development of endoplasmic reticulum stress. It is shown how these mechanisms co-operate in forming a co-ordinated rapid and prolonged compensatory antioxidant response system. • Oxidative and nitrosative stress (O&NS) are observed in neuroprogressive disorders. • Inflammation also contributes to the pathophysiology of neuroprogressive disorders. • Several counter-regulatory mechanisms that may protect brain cells in the context of O&NS and neuroinflammation. • The existence of a compensatory anti-oxidant response system is proposed. [ABSTRACT FROM AUTHOR]
- Published
- 2019
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