1. Prostaglandin D2-glycerol ester decreases carrageenan-induced inflammation and hyperalgesia in mice.
- Author
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Buisseret, Baptiste, Guillemot-Legris, Owein, Muccioli, Giulio G., and Alhouayek, Mireille
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CARRAGEENANS , *HYPERALGESIA , *PROSTAGLANDINS - Abstract
Abstract Pain is one of the cardinal signs of inflammation and is present in many inflammatory conditions. Therefore, anti-inflammatory drugs such as NSAIDs also have analgesic properties. We previously showed that prostaglandin D 2 -glycerol ester (PGD 2 -G), endogenously produced by cyclooxygenase-2 from the endocannabinoid 2-arachidonoylglycerol, has anti-inflammatory effects in vitro and in vivo that are partly mediated by DP1 receptor activation. In this work, we investigated its effect in a model of carrageenan-induced inflammatory pain. PGD 2 -G decreased hyperalgesia and edema, leading to a faster recovery. Moreover, PGD 2 -G decreased carrageenan-induced inflammatory markers in the paw as well as inflammatory cell recruitment. The effects of PGD 2 -G were independent from metabolite formation (PGD 2 and 15d-PGJ 2 -G) or DP1 receptor activation in this model. Indeed PGD 2 delayed recovery from hyperalgesia while 15d-PGJ 2 -G worsened the edema. However, while PGD 2 -G decreased hyperalgesia in this model of inflammatory pain, it had no effect when tested in the capsaicin-induced pain model. While the targets mediating the effects of this bioactive lipid in inflammatory pain remain to be elucidated, our findings further support the interest of anti-inflammatory lipid mediators in the management of inflammatory pain. Highlights • PGD 2 -G decreases inflammation and pain in carrageenan-induced hyperalgesia in mice. • These effects are not due to hydrolysis of PGD 2 -G into PGD 2. • 15d-PGJ 2 -G, a PGD 2 -G metabolite, does not mediate its effects on inflammatory pain. • PGD 2 -G decreases macrophage activation and neutrophil infiltration in the paw. • The targets mediating the effects of PGD 2 -G remain to be elucidated. [ABSTRACT FROM AUTHOR]
- Published
- 2019
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