12 results on '"Tsioka, Katerina"'
Search Results
2. Recommendations for the introduction of metagenomic next-generation sequencing in clinical virology, part II: bioinformatic analysis and reporting
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de Vries, Jutte J.C., Brown, Julianne R., Couto, Natacha, Beer, Martin, Le Mercier, Philippe, Sidorov, Igor, Papa, Anna, Fischer, Nicole, Oude Munnink, Bas B., Rodriquez, Christophe, Zaheri, Maryam, Sayiner, Arzu, Hönemann, Mario, Pérez-Cataluña, Alba, Carbo, Ellen C., Bachofen, Claudia, Kubacki, Jakub, Schmitz, Dennis, Tsioka, Katerina, Matamoros, Sébastien, Höper, Dirk, Hernandez, Marta, Puchhammer-Stöckl, Elisabeth, Lebrand, Aitana, Huber, Michael, Simmonds, Peter, Claas, Eric C.J., and López-Labrador, F. Xavier
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- 2021
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3. Isolation and whole-genome sequencing of a Crimean-Congo hemorrhagic fever virus strain, Greece.
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Papa, Anna, Papadopoulou, Elpida, Tsioka, Katerina, Kontana, Anastasia, Pappa, Styliani, Melidou, Ageliki, and Giadinis, Nektarios D.
- Abstract
Crimean-Congo hemorrhagic fever virus (CCHFV) was isolated from a pool of two adult Rhipicephalus bursa ticks removed from a goat in 2015 in Greece. The strain clusters into lineage Europe 2 representing the second available whole-genome sequenced isolate of this lineage. CCHFV IgG antibodies were detected in 8 of 19 goats of the farm. Currently CCHFV is not associated with disease in mammals other than humans. Studies in animal models are needed to investigate the pathogenicity level of lineage Europe 2 and compare it with that of other lineages. [ABSTRACT FROM AUTHOR]
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- 2018
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4. Crimean-Congo hemorrhagic fever virus in ticks collected from livestock in Albania.
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Papa, Anna, Tsioka, Katerina, Kontana, Anastasia, Velo, Enkeleda, Kadiaj, Perparim, Kota, Majlinda, and Bino, Silvia
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HEMORRHAGIC fever , *LIVESTOCK diseases , *IXODIDAE , *HYALOMMA , *GLYCOPROTEINS - Abstract
Albania is a Balkan country endemic for Crimean-Congo hemorrhagic fever (CCHF). It was shown previously that CCHF virus (CCHFV) sequences from Albanian patients cluster into Europe 1 clade. Aim of the present study was to test for CCHFV ticks collected in several regions of Albania, and to determine the genetic lineage(s) of the CCHFV strains in relation with their geographic distribution. A total of 726 ticks (366 Hyalomma marginatum , 349 Rhipicephalus bursa and 11 Rhipicephalus sanguineus ) collected from livestock during 2007–2014 were included in the study. Thirty of 215 (13.9%) tick pools were positive for CCHFV. Lineage Europe 1 was detected in H. marginatum ticks collected in the endemic region of Albania, while lineage Europe 2 was detected mainly in R. bursa ticks in various regions of the country. Both genetic lineages were detected in the CCHF endemic area (northeastern Albania), while only Europe 2 lineage was detected in the south of the country. A higher genetic diversity was seen among Europe 2 than Europe 1 Albanian sequences (mean distance 3.7% versus 1%), suggesting a longer evolution of AP92-like strains (Europe 2) in their tick hosts. The present study shows that besides CCHFV lineage Europe 1, lineage Europe 2 is also present in Albania. Combined with results from recent studies, it is concluded that lineage Europe 2 is widely spread in the Balkans and Turkey, and is associated mainly with R. bursa ticks (at least in this region). Its pathogenicity and impact to the public health remain to be elucidated. [ABSTRACT FROM AUTHOR]
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- 2017
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5. Novel phlebovirus detected in Haemaphysalis parva ticks in a Greek island.
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Papa, Anna, Kontana, Anastasia, Tsioka, Katerina, Saratsis, Anastasios, and Sotiraki, Smaragda
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During the last decade the number of novel tick-borne phleboviruses has increased rapidly, especially after the identification of severe fever with thrombocytopenia syndrome and Heartland viruses which can cause severe disease in humans. A novel virus, Antigone virus was recently detected in ticks collected from the mainland of Greece. The aim of the present study was to investigate the presence of tick-borne phleboviruses in an island in Greece. During November 2015, 31 ticks were collected from sheep in Lesvos island. Phleboviral RNA was detected in 12/22 adult Haemaphysalis parva ticks. The virus was provisionally named Lesvos virus after the name of the island. Phylogenetic analysis of a 1108-bp L RNA fragment revealed that the Lesvos virus sequences cluster together with Dabieshan and Yongjia tick viruses detected in China in H. longicornis and H. hystricis ticks, respectively. Further studies are needed to investigate its exact distribution, epidemiology and virulence. It is expected that the research studies on tick biology and pathogen-tick-host interactions will allow a better understanding of the virus life cycle and the elucidation of the possible role of the novel tick-borne phleboviruses in public health. [ABSTRACT FROM AUTHOR]
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- 2017
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6. Bacterial pathogens and endosymbionts in ticks.
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Papa, Anna, Tsioka, Katerina, Kontana, Anastasia, Papadopoulos, Christos, and Giadinis, Nektarios
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Ticks collected from goats in northern Greece were tested for the presence of tick-borne bacteria. Among adult ticks, 37 (57.8%) were Rhipicephalus bursa , 11 (17.2%) Dermacentor marginatus, 10 (15.6%) Ixodes ricinus , 3 (4.7%) Rhipicephalus sanguineus sensu lato and 2 (3.1%) Haemaphysalis parva ; one (1.6%) Rhipicephalus spp. tick was nymph. Rickettsia monacensis , Rickettsia massilae , Anaplasma phagocytophilum and Anaplasma platys were detected in I. ricinus and Rh. bursa ticks. A variety of Coxiella -like endosymbionts were detected in all tick genera tested, forming distinct clades from Coxiella burnetii in the phylogenetic tree based on the 16S rRNA gene. An additional endosymbiont, Candidatus Midichloria mitochondrii, was detected in most of the I. ricinus ticks. Surveillance for human pathogens in ticks provides knowledge helpful for the public health, while further studies are needed to determine the role of endosymbionts in tick physiology, vector competence and probably in public health. [ABSTRACT FROM AUTHOR]
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- 2017
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7. Novel phleboviruses detected in ticks, Greece.
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Papa, Anna, Kontana, Anastasia, Tsioka, Katerina, Chaligiannis, Ilias, and Sotiraki, Smaragda
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Since 2009, when severe fever with thrombocytopenia syndrome virus and Heartland virus have been identified and associated with disease in humans, the interest on tick-borne phleboviruses is increasing rapidly. The aim of the present study was to investigate the presence of tick-borne phleboviruses in Greece and compare them with respective ones detected worldwide. Ticks collected from goats and sheep in 60 sites of 13 regional units of Greece were grouped in pools (1–3 ticks per pool) and tested for the presence of phleboviral RNA. Six of 210 pools were positive; they consisted of Rhipicephalus sanguineus ticks collected from sheep in 3 regional units of Greece: Pella (2/30, 6.7%), Imathia (2/21, 9.5%), and Ioannina (2/28, 7.1%). The overall tick minimum infection rate was 2.1%. The sequences of the Greek phlebovirus (provisionally named Antigone virus) form a distinct clade in the tick-borne phleboviruses, differing by >40% from the currently known phleboviruses. Any probable implication of these viruses to public health remains to be elucidated. [ABSTRACT FROM AUTHOR]
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- 2016
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8. A novel AP92-like Crimean-Congo hemorrhagic fever virus strain, Greece.
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Papa, Anna, Chaligiannis, Ilias, Kontana, Natasa, Sourba, Tatiana, Tsioka, Katerina, Tsatsaris, Andreas, and Sotiraki, Smaragda
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Ticks were collected from various regions of northern Greece and tested for the presence of Crimean-Congo hemorrhagic fever virus (CCHFV) RNA. Human and animal sera were collected in the regions where CCHFV-positive ticks were detected, and they were tested for the presence of IgG antibodies against the virus. A CCHFV strain was detected in Rhipicephalus bursa ticks collected from sheep in Kastoria regional unit, differing by 9.7% at the nucleotide level from the AP92 strain, which was isolated in 1975 in another region of Greece. Up to date, CCHF cases have not been reported in these regions. The human seroprevalence in the area was estimated at 6%, while IgG-positive sheep was detected in two of the four neighboring farms tested. The circulation of this specific CCHFV lineage in Greece, especially in a region where the seroprevalence is high, together with the lack of human CCHF cases, suggests a probable antigenic, but non- or low-pathogenic character of this lineage. Further studies on these strains will increase our knowledge about the role of AP92-like strains in the CCHF epidemiology, which might be useful for drug and vaccine design. [ABSTRACT FROM AUTHOR]
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- 2014
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9. West Nile fever upsurge in a Greek regional unit, 2020.
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Papa, Anna, Tsioka, Katerina, Gewehr, Sandra, Kalaitzopouou, Stella, Pervanidou, Danai, Vakali, Anna, Kefaloudi, Chrysovalantou, Pappa, Styliani, Louka, Xanthoula, and Mourelatos, Spiros
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WEST Nile fever , *WEST Nile virus , *CULEX pipiens , *CULEX , *BODIES of water - Abstract
• Serres regional unit in Greece was highly affected by West Nile virus in 2020. • The first human case occurred nearby a wetland. • Ηuman cases and WNV-positive mosquitoes were detected nearby water bodies. • The WNV detection rate in Culex pipiens mosquito pools was 14.3%. During the 2020 West Nile virus (WNV) transmission season, Greece was the most affected EU Member State. More than one third of human cases occurred in Serres regional unit in northern Greece, which is characterized by the presence of a major wetland (Kerkini lake and Strimon river). A total of 2809 Culex pipiens mosquitoes collected in Serres were grouped into 70 pools and tested for WNV. Ten (14.3%) pools were found positive, and all WNV sequences belonged to the Central European subclade of WNV lineage 2. The first human case occurred in a village nearby the lake, and all following cases occurred across the connected river and its tributaries. Similar distribution presented the sites where WNV-positive mosquitoes were detected. The number of Culex spp. mosquitoes per trap per night was higher in 2020 than in previous years (2017-2019). The spatial and temporal distribution of human cases and WNV-positive mosquitoes in 2020 in Serres regional unit suggest that the upsurge of the virus circulation was probably related with factors that affected the ecosystem of the wetland. [Display omitted] [ABSTRACT FROM AUTHOR]
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- 2021
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10. West Nile virus lineage 2 in Culex mosquitoes in Thessaly, Greece, 2019.
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Papa, Anna, Tsioka, Katerina, Gewehr, Sandra, Kalaitzopoulou, Stella, Pappa, Styliani, and Mourelatos, Spiros
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WEST Nile virus , *CULEX , *MOSQUITOES , *NUCLEOTIDE sequencing , *AEDES aegypti - Abstract
West Nile virus is a flavivirus transmitted to humans mainly by mosquito bites. Outbreaks are observed in several European countries, and Greece is one of the most affected countries during the recent years. Thessaly was one of the most affected regions in Greece in 2019. A total of 3,025 Culex spp. mosquitoes collected in Thessaly were grouped into 47 pools and tested for West Nile virus (WNV). Eight (17%) pools were found positive. Whole genome sequences were obtained from two positive pools. Phylogenetic analysis showed that the causative strain was an evolutionary variant of the strains circulating in 2018 belonging to the Balkan subgroup of the Central European subclade of WNV lineage 2. [ABSTRACT FROM AUTHOR]
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- 2020
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11. Molecular epidemiology of Dobrava-Belgrade virus in Greece.
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Tsergouli, Katerina, Papadopoulou, Elpida, Tsioka, Katerina, and Papa, Anna
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VIRUS phylogeny , *MOLECULAR epidemiology , *NUCLEOTIDE sequence , *VIRAL genetics , *HEMORRHAGIC fever with renal syndrome - Abstract
Abstract In order to gain an insight into the genetic relatedness of the Dobrava-Belgrade virus (DOBV) in Greece, a phylogenetic analysis was performed based on all currently available DOBV sequences obtained from hospitalized cases with hemorrhagic fever with renal syndrome (HFRS). Most cases occurred in northwestern and north central part of the country. Two sequence datasets consisted of 41 S and 12 M partial DOBV RNA segment sequences were analyzed. All DOBV strains belong to Dobrava genotype which is associated with the rodent Apodemus flavicollis. In both phylogenetic trees (S and M segments), two main clusters of Greek strains could be distinguished. Phylogenetic analysis showed a spatial rather than temporal relation of the strains, since their genetic clustering was highly associated with the geographic distribution of the cases. Besides previous characterized endemic foci, novel ones have been identified, expanding our knowledge on the epidemiology of HFRS in Greece. Highlights • Most HFRS cases in Greece occur in the northern part of the country. • Dobrava-Belgrade virus strains in Greece belong to the Dobrava genotype. • Greek sequences cluster into two main phylogenetic groups. • A spatial rather than temporal relation is seen among strains. [ABSTRACT FROM AUTHOR]
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- 2018
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12. Detection of flaviviruses and alphaviruses in mosquitoes in Central Macedonia, Greece, 2018.
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Papa, Anna, Gewehr, Sandra, Tsioka, Katerina, Kalaitzopoulou, Stella, Pappa, Styliani, and Mourelatos, Spiros
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FLAVIVIRUSES , *ALPHAVIRUSES , *WEST Nile virus , *AEDES aegypti , *MOSQUITOES , *VETERINARY public health , *CULEX , *MOSQUITO vectors - Abstract
Culex mosquitoes are vectors of several flaviviruses and alphaviruses posing a potential risk to public and veterinary health. In order to gain an insight into the flaviviruses and alphaviruses circulating in the five regional units of Central Macedonia in northern Greece, 17,470 female Culex spp. mosquitoes collected during 2018 were tested for these viruses. Among 229 mosquito pools, West Nile virus (WNV) was detected in 10 (4.4%) pools, while insect-specific flavi- and alphaviruses were detected in 2 (0.9%) and 8 (3.5%) pools, respectively. WNV minimum infection rate (MIR) was 0.57. The highest MIR was identified in Thessaloniki regional unit, where several human cases of WNV infection occurred in 2018. All ten WNV sequences cluster into the Central European subclade of lineage 2. It is of note that the first WNV-positive mosquito pool was detected two weeks prior the report of the first human case in the area, suggesting that testing of mosquitoes could serve as early warning system. Image, graphical abstract [ABSTRACT FROM AUTHOR]
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- 2020
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