11 results on '"Treuter, Eckardt"'
Search Results
2. Ligand-independent actions of the orphan receptors/corepressors DAX-1 and SHP in metabolism, reproduction and disease
- Author
-
Ehrlund, Anna and Treuter, Eckardt
- Subjects
- *
NUCLEAR receptors (Biochemistry) , *CHOLESTEROL metabolism , *HOMEOSTASIS , *LIGANDS (Biochemistry) , *METABOLISM , *REPRODUCTION , *GENETIC transcription - Abstract
Abstract: DAX-1 and SHP are two closely related atypical orphan members of the nuclear receptor (NR) family that make up the NR0B subfamily. They combine properties of typical NRs and of NR-associated coregulators: both carry the characteristic NR ligand-binding domain but instead of a NR DNA-binding domain they have unique N-terminal regions that contain LxxLL-related NR-binding motifs often found in coregulators. Recent structural data indicate that DAX-1 lacks a ligand-binding pocket and thus should rely on ligand-independent mechanisms of regulation. This might be true, but remains to be proven, for SHP as well. DAX-1 and SHP have in common that they act as transcriptional corepressors of cholesterol metabolism pathways that are related on a molecular level. However, the expression patterns of the two NRs are largely different, with some notable exceptions, and so are the physiological processes they regulate. DAX-1 is mainly involved in steroidogenesis and reproductive development, while SHP plays major roles in maintaining cholesterol and glucose homeostasis. This review highlights the key similarities and differences between DAX-1 and SHP with regard to structure, function and biology and considers what can be learnt from recent research advances in the field. This article is part of a Special Issue entitled ‘Orphan Receptors’. [Copyright &y& Elsevier]
- Published
- 2012
- Full Text
- View/download PDF
3. Liver X receptor biology and pharmacology: new pathways, challenges and opportunities
- Author
-
Jakobsson, Tomas, Treuter, Eckardt, Gustafsson, Jan-Åke, and Steffensen, Knut R.
- Subjects
- *
NUCLEAR receptors (Biochemistry) , *PHARMACOLOGY , *HOMEOSTASIS , *LIGANDS (Biochemistry) , *OXYSTEROLS , *INFLAMMATION , *AUTOIMMUNITY - Abstract
Nuclear receptors (NRs) are master regulators of transcriptional programs that integrate the homeostatic control of almost all biological processes. Their direct mode of ligand regulation and genome interaction is at the core of modern pharmacology. The two liver X receptors LXRα and LXRβ are among the emerging newer drug targets within the NR family. LXRs are best known as nuclear oxysterol receptors and physiological regulators of lipid and cholesterol metabolism that also act in an anti-inflammatory way. Because LXRs control diverse pathways in development, reproduction, metabolism, immunity and inflammation, they have potential as therapeutic targets for diseases as diverse as lipid disorders, atherosclerosis, chronic inflammation, autoimmunity, cancer and neurodegenerative diseases. Recent insights into LXR signaling suggest future targeting strategies aiming at increasing LXR subtype and pathway selectivity. This review discusses the current status of our understanding of LXR biology and pharmacology, with an emphasis on the molecular aspects of LXR signaling that constitute the potential of LXRs as drug targets. [ABSTRACT FROM AUTHOR]
- Published
- 2012
- Full Text
- View/download PDF
4. Transcriptional control of metabolic and inflammatory pathways by nuclear receptor SUMOylation
- Author
-
Treuter, Eckardt and Venteclef, Nicolas
- Subjects
- *
NUCLEAR receptors (Biochemistry) , *UBIQUITIN , *GENETIC regulation , *ACUTE phase reaction , *METABOLISM , *POST-translational modification , *INFLAMMATION - Abstract
Abstract: Nuclear receptors (NRs) exert crucial functions in controlling metabolism and inflammation by both positively and negatively regulating gene expression. Recent evidence suggests that the transcriptional activities of many NRs can be modulated and even re-directed through post-translational modification by small ubiquitin-related modifiers (SUMO). SUMOylation triggers a plethora of diverse molecular events that can alter both the fate and function of modified NRs at the nongenomic, genomic, and epigenomic level. However, it is the intriguing link of SUMOylation to transcriptional repression, and in particular to transrepression, that has emerged as a common underlying mechanism that impacts on biological processes controlled by NRs. It further appears that the cell-type-specific SUMOylation status of NRs can be regulated by ligands and by signal-dependent crosstalk of post-translational modifications. Given the causal role of altered NR signaling in the development and pathogenesis of human diseases, it is likely that aberrant SUMO conjugation, deconjugation, or interpretation contributes to these alterations. Here, we review the current progress made in both the study and understanding of the molecular mechanisms and consequences of NR SUMOylation and also discuss the physiological and pharmacological implications with a particular focus on transrepression pathways that link metabolism and inflammation. This article is part of a Special Issue entitled: Translating nuclear receptors from health to disease. [Copyright &y& Elsevier]
- Published
- 2011
- Full Text
- View/download PDF
5. Loss of G protein pathway suppressor 2 in human adipocytes triggers lipid remodeling by upregulating ATP binding cassette subfamily G member 1.
- Author
-
Barilla, Serena, Liang, Ning, Mileti, Enrichetta, Ballaire, Raphaëlle, Lhomme, Marie, Ponnaiah, Maharajah, Lemoine, Sophie, Soprani, Antoine, Gautier, Jean-Francois, Amri, Ez-Zoubir, Le Goff, Wilfried, Venteclef, Nicolas, and Treuter, Eckardt
- Abstract
Adipogenesis is critical for adipose tissue remodeling during the development of obesity. While the role of transcription factors in the orchestration of adipogenic pathways is already established, the involvement of coregulators that transduce regulatory signals into epigenome alterations and transcriptional responses remains poorly understood. The aim of our study was to investigate which pathways are controlled by G protein pathway suppressor 2 (GPS2) during the differentiation of human adipocytes. We generated a unique loss-of-function model by RNAi depletion of GPS2 in human multipotent adipose-derived stem (hMADS) cells. We thoroughly characterized the coregulator depletion-dependent pathway alterations during adipocyte differentiation at the level of transcriptome (RNA-seq), epigenome (ChIP-seq H3K27ac), cistrome (ChIP-seq GPS2), and lipidome. We validated the in vivo relevance of the identified pathways in non-diabetic and diabetic obese patients. The loss of GPS2 triggers the reprogramming of cellular processes related to adipocyte differentiation by increasing the responses to the adipogenic cocktail. In particular, GPS2 depletion increases the expression of BMP4 , an important trigger for the commitment of fibroblast-like progenitors toward the adipogenic lineage and increases the expression of inflammatory and metabolic genes. GPS2-depleted human adipocytes are characterized by hypertrophy, triglyceride and phospholipid accumulation, and sphingomyelin depletion. These changes are likely a consequence of the increased expression of ATP-binding cassette subfamily G member 1 (ABCG1) that mediates sphingomyelin efflux from adipocytes and modulates lipoprotein lipase (LPL) activity. We identify ABCG1 as a direct transcriptional target, as GPS2 depletion leads to coordinated changes of transcription and H3K27 acetylation at promoters and enhancers that are occupied by GPS2 in wild-type adipocytes. We find that in omental adipose tissue of obese humans, GPS2 levels correlate with ABCG1 levels, type 2 diabetic status, and lipid metabolic status, supporting the in vivo relevance of the hMADS cell-derived in vitro data. Our study reveals a dual regulatory role of GPS2 in epigenetically modulating the chromatin landscape and gene expression during human adipocyte differentiation and identifies a hitherto unknown GPS2-ABCG1 pathway potentially linked to adipocyte hypertrophy in humans. • GPS2 depletion in human adipose-derived mesenchymal stem cells increases expression of adipogenic genes, including BMP4. • Loss of GPS2 leads to coordinated changes of epigenome and transcriptome during human adipocyte differentiation. • Loss of GPS2 upregulates ABCG1 and LPL and induces lipidome remodeling including sphingomyelin depletion. • The GPS2-ABCG1 pathway contributes to adipocyte hypertrophy. • GPS2 and ABCG1 levels in omental adipose tissue inversely correlate with type 2 diabetes in obese humans. [ABSTRACT FROM AUTHOR]
- Published
- 2020
- Full Text
- View/download PDF
6. Adipocyte Reprogramming by the Transcriptional Coregulator GPS2 Impacts Beta Cell Insulin Secretion.
- Author
-
Drareni, Karima, Ballaire, Raphaëlle, Alzaid, Fawaz, Goncalves, Andreia, Chollet, Catherine, Barilla, Serena, Nguewa, Jean-Louis, Dias, Karine, Lemoine, Sophie, Riveline, Jean-Pierre, Roussel, Ronan, Dalmas, Elise, Velho, Gilberto, Treuter, Eckardt, Gautier, Jean-François, and Venteclef, Nicolas
- Abstract
Glucose homeostasis is maintained through organ crosstalk that regulates secretion of insulin to keep blood glucose levels within a physiological range. In type 2 diabetes, this coordinated response is altered, leading to a deregulation of beta cell function and inadequate insulin secretion. Reprogramming of white adipose tissue has a central role in this deregulation, but the critical regulatory components remain unclear. Here, we demonstrate that expression of the transcriptional coregulator GPS2 in white adipose tissue is correlated with insulin secretion rate in humans. The causality of this relationship is confirmed using adipocyte-specific GPS2 knockout mice, in which inappropriate secretion of insulin promotes glucose intolerance. This phenotype is driven by adipose-tissue-secreted factors, which cause increased pancreatic islet inflammation and impaired beta cell function. Thus, our study suggests that, in mice and in humans, GPS2 controls the reprogramming of white adipocytes to influence pancreatic islet function and insulin secretion. • GPS2 expression in adipose tissue is associated with insulin secretion rate in humans • Loss of GPS2 in adipocytes impacts on insulin secretion upon diet-induced obesity • Beta cell dysfunction in Gps2 KO mice is governed by islet inflammation • This beta cell maladaptation in Gps2 KO mice is mediated by adipose tissue secretome Appropriate insulin secretion is governed through organ crosstalk. Drareni et al. show that GPS2 expression in adipose tissue is correlated with insulin secretion rate in humans. The causality of this relationship is confirmed using adipocyte-specific GPS2 knockout mice, in which inappropriate secretion of insulin promotes glucose intolerance in obese mice. [ABSTRACT FROM AUTHOR]
- Published
- 2020
- Full Text
- View/download PDF
7. GPS2 Deficiency Triggers Maladaptive White Adipose Tissue Expansion in Obesity via HIF1A Activation.
- Author
-
Drareni, Karima, Ballaire, Raphaëlle, Barilla, Serena, Mathew, Mano J., Toubal, Amine, Fan, Rongrong, Liang, Ning, Chollet, Catherine, Huang, Zhiqiang, Kondili, Maria, Foufelle, Fabienne, Soprani, Antoine, Roussel, Ronan, Gautier, Jean-François, Alzaid, Fawaz, Treuter, Eckardt, and Venteclef, Nicolas
- Abstract
Summary Hypertrophic white adipose tissue (WAT) represents a maladaptive mechanism linked to the risk for developing type 2 diabetes in humans. However, the molecular events that predispose WAT to hypertrophy are poorly defined. Here, we demonstrate that adipocyte hypertrophy is triggered by loss of the corepressor GPS2 during obesity. Adipocyte-specific GPS2 deficiency in mice (GPS2 AKO) causes adipocyte hypertrophy, inflammation, and mitochondrial dysfunction during surplus energy. This phenotype is driven by HIF1A activation that orchestrates inadequate WAT remodeling and disrupts mitochondrial activity, which can be reversed by pharmacological or genetic HIF1A inhibition. Correlation analysis of gene expression in human adipose tissue reveals a negative relationship between GPS2 and HIF1A, adipocyte hypertrophy, and insulin resistance. We propose therefore that the obesity-associated loss of GPS2 in adipocytes predisposes for a maladaptive WAT expansion and a pro-diabetic status in mice and humans. Graphical Abstract Highlights • Adipose-specific GPS2 deficiency predisposes for adipocyte hypertrophy • Loss of GPS2 triggers transcriptional activation of HIF1A pathways • Deregulation of GPS2-HIF1A interplay provokes disrupted mitochondrial activity • GPS2 and HIF1A levels are negatively correlated in human adipose tissue Drareni et al. identify a role for the transcriptional corepressor GPS2 in the regulation of adipocyte hypertrophy. They provide evidence that adipocyte-specific loss of GPS2 predisposes toward maladaptive adipose tissue expansion and pro-diabetic status through activation of HIF1A transcriptional activity. [ABSTRACT FROM AUTHOR]
- Published
- 2018
- Full Text
- View/download PDF
8. An Alternative Splicing Variant of the Selenoprotein Thioredoxin Reductase Is a Modulator of Estrogen Signaling.
- Author
-
Damdimopoulos, Anastasios E., Miranda-Vizuete, Antonio, Treuter, Eckardt, Gustafsson, Ejan-Åke, and Spyrou, Giannis
- Subjects
- *
PROTEINS , *THIOREDOXIN , *TRANSCRIPTION factors , *CELL receptors , *ESTROGEN receptors , *CONFOCAL microscopy - Abstract
The selenoprotein thioredoxin reductase (TrxR1) is an integral part of the thioredoxin system. It serves to transfer electrons from NADPH to thioredoxin leading to its reduction. Interestingly, recent work has indicated that thioredoxin reductase can regulate the activity of transcription factors such as p53, hypoxia-inducible factor, and AP-1. Here, we describe that an alternative splicing variant of thioredoxin reductase (TrxR1b) containing an LXXLL peptide motif, is implicated in direct binding to nuclear receptors. In vitro interaction studies revealed direct interaction of the TrxR1b with the estrogen receptors α and β. Confocal microscopy analysis showed nuclear colocalization of the TrxR1b with both estrogen receptor α and β in estradiol-17β-treated cells. Transcriptional studies demonstrated that TrxR1b can affect estrogen-dependent gene activation differentially at classical estrogen response elements as compared with AP-1 response elements. Based on these results, we propose a model where thioredoxin reductase directly influences the estrogen receptor-coactivator complex assembly on nonclassical estrogen response elements such as AP-1. In summary, our results suggest that TrxR1b is an important modulator of estrogen signaling. [ABSTRACT FROM AUTHOR]
- Published
- 2004
- Full Text
- View/download PDF
9. Metabolic nuclear receptor signaling and the inflammatory acute phase response
- Author
-
Venteclef, Nicolas, Jakobsson, Tomas, Steffensen, Knut R., and Treuter, Eckardt
- Subjects
- *
NUCLEAR receptors (Biochemistry) , *CELLULAR signal transduction , *INFLAMMATION , *ACUTE phase reaction , *GENE expression , *CYTOKINES , *METABOLISM , *NATURAL immunity , *LIVER cells - Abstract
The acute phase response (APR) classically refers to the rapid reprogramming of gene expression and metabolism in response to inflammatory cytokine signaling. As components of the innate immune system, hepatocyte-derived acute phase proteins (APPs) play a central role in restoring tissue homeostasis. Recently, an intriguing ‘metaflammatory’ facet of the APR became evident with chronically elevated APP levels being connected to metabolic syndrome disorders. The causality of these connections is unclear but could relate to adverse metabolic and inflammatory disturbances, particularly those affecting lipoprotein properties, cholesterol metabolism and atherogenesis. Here we review these aspects with an emphasis on the emerging importance of lipid-sensing nuclear receptors (LXRs, LRH-1, PPARs), in conjunction with anti-inflammatory transrepression pathways, as physiological and pharmacological relevant modulators of the APR. [ABSTRACT FROM AUTHOR]
- Published
- 2011
- Full Text
- View/download PDF
10. Structural Insights into Corepressor Recognition by Antagonist-bound Estrogen Receptors.
- Author
-
Heldring, Nina, Pawson, Tanya, McDonnell, Donald, Treuter, Eckardt, Gustafsson, Jan-Ake, and Pike, Ashley C. W.
- Subjects
- *
ESTROGEN antagonists , *PEPTIDES , *LIGANDS (Biochemistry) , *HORMONE antagonists , *PROTEINS - Abstract
Direct recruitment of transcriptional corepressors to estrogen receptors (ER) is thought to contribute to the tissue-specific effects of clinically important ER antagonists. Here, we present the crystal structures of two affinity-selected peptides in complex with antagonist-bound ERα ligand-binding domain. Both peptides adopt helical conformations, bind along the activation function 2 coregulator interaction surface, and mimic corepressor (CoRNR) sequence motif binding. Peptide binding is weak in a wild-type context but significantly enhanced by removal of ER helix 12. This region contains a previously unrecognized CoRNR motif that is able to compete with corepressors for binding to activation function 2, thereby providing a structural explanation for the poor ability of ER to directly interact with classical corepressors. Furthermore, the ability of other sequence motifs to mimic corepressor binding raises the possibility that coregulators do not necessarily require CoRNR motifs for direct recruitment to antagonist-bound ER. [ABSTRACT FROM AUTHOR]
- Published
- 2007
- Full Text
- View/download PDF
11. Transcriptional corepression by SHP: molecular mechanisms and physiological consequences
- Author
-
Båvner, Ann, Sanyal, Sabyasachi, Gustafsson, Jan-Åke, and Treuter, Eckardt
- Subjects
- *
GENETIC transcription , *TRANSCRIPTION factors , *CELL receptors , *NUCLEAR receptors (Biochemistry) - Abstract
Small heterodimer partner (SHP; NR0B2), an exceptional member of the mammalian nuclear receptor family, directly modulates the activities of conventional nuclear receptors by acting as an inducible and tissue-specific corepressor. Recent progress in dissecting underlying molecular mechanisms, identifying target factors and target genes, and uncovering physiological functions points to the regulatory involvement of SHP in diverse metabolic and intracellular pathways that awaits future clarification. In this review, we carry out a comprehensive survey of all published data and discuss our current understanding of molecular mechanisms and physiological consequences governing SHP action. [Copyright &y& Elsevier]
- Published
- 2005
- Full Text
- View/download PDF
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.