8 results on '"Steffensen S"'
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2. Effect of ischemic preconditioning and a Kv7 channel blocker on cardiac ischemia-reperfusion injury in rats
- Author
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Corydon, K. K., Matchkov, V., Fais, R., Abramochkin, D., Hedegaard, E. R., Comerma-Steffensen, S., Simonsen, U., Corydon, K. K., Matchkov, V., Fais, R., Abramochkin, D., Hedegaard, E. R., Comerma-Steffensen, S., and Simonsen, U.
- Abstract
Recently, we found cardioprotective effects of ischemic preconditioning (IPC), and from a blocker of KCNQ voltage-gated K+ channels (KV7), XE991 (10,10-bis(4-pyridinylmethyl)-9(10H)-anthracenone), in isolated rat hearts. The purpose of the present study was to investigate the cardiovascular effects of IPC and XE991 and whether they are cardioprotective in intact rats. In conscious rats, we measured the effect of the KV7 channel blocker XE991 on heart rate and blood pressure by use of telemetry. In anesthetized rats, cardiac ischemia was induced by occluding the left coronary artery, and the animals received IPC (2 × 5 min of occlusion), XE991, or a combination. After a 2 h reperfusion period, the hearts were excised, and the area at risk and infarct size were determined. In both anesthetized and conscious rats, XE991 increased blood pressure, and the highest dose (7.5 mg/kg) of XE991 also increased heart rate, and 44% of conscious rats died. XE991 induced marked changes in the electrocardiogram (e.g., increased PR interval and prolonged QTC interval) without changing cardiac action potentials. The infarct size to area at risk ratio was reduced from 53 ± 2% (n = 8) in the vehicle compared to 36 ± 3% in the IPC group (P < 0.05, n = 9). XE991 (0.75 mg/kg) treatment alone or on top of IPC failed to reduce myocardial infarct size. Similar to the effect in isolated hearts, locally applied IPC was cardioprotective in intact animals exposed to ischemia-reperfusion. Systemic administration of XE991 failed to protect the heart against ischemia-reperfusion injury suggesting effects on the autonomic nervous system counteracting the cardioprotection in intact animals. © 2019 Elsevier B.V.
- Published
- 2020
3. Acupuncture at HT7 Inhibits Reinforcing Effects of Methamphetamine
- Author
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Lee, B.H., Yang, C.H., Kim, H.Y., Jang, E.Y., Chang, S., Kim, N.J., Ryu, Y., Fan, Y., Gwak, Y., Bills, K., Steffensen, S., and Koo, J.S.
- Published
- 2018
- Full Text
- View/download PDF
4. Debonding of particles in thin films.
- Author
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Steffensen, S., Kibsgaard, R.L., and Jensen, H.M.
- Subjects
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DEBONDING , *PARTICLES , *MECHANICAL wear , *THIN films , *COATING processes , *RESIDUAL stresses - Abstract
Abstract: Thin composite films consisting of a matrix with embedded particles are currently being developed both as hard, wear resistant coatings and as functional surfaces. The effect of stiff particles in the film are studied for systems where the film is under residual tensile stresses. The particles, when they are fully bonded to the matrix, increase the stiffness of the composite film. In cases where the particles debond from the matrix material, the stiffness of the composite film decreases. The conditions under which the debonding process is stable are studied. For systems properly designed, a controlled debonding process of the particles can thus be used to reduce the stress levels in composite film lowering the risk for delamination of the composite film from the substrate as well as the risk of through cracks in the film. The work includes finite element based unit cell calculations of interface debonding between spherical particles and the film, and the release of residual stresses following this. The three dimensional unit cell calculations assume a periodic distribution of particles in the plane parallel to the substrate interface with equi-biaxial tension and periodicity with zero overall stress perpendicular to the substrate interface. [Copyright &y& Elsevier]
- Published
- 2014
- Full Text
- View/download PDF
5. Energy release rate for circular crack due to indentation in a brittle film on a ductile substrate.
- Author
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Steffensen, S. and Jensen, H.M.
- Subjects
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SURFACE cracks , *THIN films , *MECHANICAL models , *FRACTURE mechanics , *ESTIMATION theory , *BRITTLENESS - Abstract
Abstract: A recent method by Steffensen et al. (2013) to estimate the fracture toughness of thin, hard films uses closed-form linear elastic fracture mechanical models for an edge crack in a semi-infinite plane to calculate the energy release rate for a circumferential crack propagating from the surface of the film. Also, in the proposed model the in-plane shear stress was neglected, and it was assumed that the crack propagates perpendicular to the surface. In this paper, the accuracy of the previously proposed method for calculating the energy release rate for axisymmetric circumferential crack during indentation is investigated. Also, neglecting the in-plane shear stress and the assumed direction of crack propagation are investigated. The J-integral and the virtual crack closure technique are used to calculate the energy release rate on the basis of results from a large scale axisymmetric finite element model. Thereby, the proper geometry of the circumferential crack, plasticity in the substrate, material mismatch across the interface are included. In general, it was found that the closed-form linear elastic fracture mechanics model is sufficient for the method. [Copyright &y& Elsevier]
- Published
- 2014
- Full Text
- View/download PDF
6. Numerical estimation of fracture toughness from indentation-induced circumferential cracking in thin films on ductile substrates.
- Author
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Steffensen, S., Madsen, N.D., and Jensen, H.M.
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FRACTURE toughness , *PARAMETER estimation , *FINITE element method , *MATERIAL plasticity , *THIN films , *DUCTILE fractures , *COMPUTER simulation , *STRAINS & stresses (Mechanics) - Abstract
Abstract: In this paper, a method to estimate fracture toughness, , from the first indenter-induced circumferential crack in a thin film is suggested. The method is based on the stresses in the film and finite element simulations are used to estimate these stresses. The film is taken to be linear elastic while plasticity is included in the substrate. Delamination is not taken into account in the method and the film is perfectly bonded to the substrate. The stress in the film is used to estimate the location where the circumferential crack will initiate from a defect in the film surface. The location is found to be at a distance away from the indenter which correlate with experiments. Also, the stress is used to calculate the stress intensity factor and the energy release rate for a plane strain crack. The energy release rate for the plane strain crack is then used to calculate the energy release for a crack channeling around the indenter forming the circumferential crack. By the energy release rate for the channeling crack fracture toughness is estimated independent of the initial defects in the film provided such defects exist. If the initial defects are smaller than a required minimum value, fracture toughness is overestimated by this method and this overestimation is calculated. Besides fracture toughness, the energy release rate for the channeling crack is used to estimate the depth of the plane strain crack which is found to be smaller than the film thickness. The method is used to estimate the fracture toughness, , for an alumina ( ) film using the critical indentation depth. In order to verify the result the estimated crack radius is compared to the actual radius of the circumferential crack in the alumina. The differences between the two radius is found to be within a few percent. [Copyright &y& Elsevier]
- Published
- 2013
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7. Effect of hypoxia/reoxygenation on the contractility of the isolated bovine digital vein
- Author
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Risso, A., Comerma-Steffensen, S., Rojas, J., Ascanio, E., Hahn, M., Reggio, M., and Zerpa, H.
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HYPERBARIC oxygenation , *CONTRACTILITY (Biology) , *BLOOD vessels , *LAMINITIS , *PATHOLOGICAL physiology , *HEMODYNAMICS , *ENDOTHELIUM - Abstract
Abstract: The bovine digital vasculature contractility has been implicated in the development of laminitis. To investigate the effect of hypoxia/reoxygenation on the contractility of isolated peripheral bovine digital veins (BDVs), vessel rings were studied under isometric conditions and submitted to 30min of hypoxia (95%N2–5%CO2) and reoxygenation (95%O2–5%CO2) conditions, respectively. The BDVs contracted with a high K+ depolarizing solution, developed hypoxia-induced relaxation, followed by an increase in tension upon reoxygenation. In contrast, phenylephrine-contracted BDVs displayed a rapid, sustained and reversible hypoxia-induced contraction. Reoxygenation caused a rapid relaxation in phenylephrine-contracted BDVs. The presence of the endothelium did not modify the hypoxia/reoxygenation effects and hypoxia-induced contraction was still observed in a nominal Ca2+-free Krebs, however, the last effect was not maintained over time. The hypoxia-induced contraction in an isolated peripheral vein may contribute to the understanding of the physiology and pathophysiology of superficial venous smooth muscle contractility, particularly in the alteration of bovine digital haemodynamics under hypoxia/reoxygenation conditions. [Copyright &y& Elsevier]
- Published
- 2012
- Full Text
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8. Haematological values of post-laying Arrau turtle (Podocnemis expansa) in the Orinoco River, Venezuela
- Author
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Rossini, M., Blanco, P.A., Marín, E., Comerma-Steffensen, S., and Zerpa, H.
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HEMATOLOGY , *PODOCNEMIS expansa , *HEMOGLOBINS , *BLOOD platelets , *ERYTHROCYTES , *LEUCOCYTES - Abstract
Abstract: The Arrau turtle (Podocnemis expansa) is an endangered species, as a result of long-lasting, unsustainable exploitation. To obtain reference haematological values from the wild Podocnemis expansa during post-laying, 20 turtles were captured in the Orinoco River. Blood was obtained from the dorsal cervical sinus in lithium heparin tubes. Red blood cells (RBC), white blood cells (WBC), thrombocytes (TC), packed cell volume (PCV), plasmatic protein (PP), haemoglobin (Hgb), mean corpuscular volume (MCV) and differential leukocyte count were determined. Haematological values were: RBC 0.9×109/L, WBC 5.7×109/L, TC 5.4×109/L, PCV 35.6%, PP 4.2g/dL, Hgb 11.8g/dL, MCV 411fL. The differential leukocyte count comprised: 71% heterophils, 23% lymphocytes, 3% eosinophils, 1.6% basophils, and 1% monocytes. The reports of reference haematology values for the wild P. expansa are limited; therefore, the results presented herein contrast with those values obtained in captivity. This study represents a contribution to the referential haematological values of the wild P. expansa. [Copyright &y& Elsevier]
- Published
- 2012
- Full Text
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