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Abstract: Objectives:: The analytical variability of the Fibrotest (FT) parameters raises the issue of the test''s reliability for routine use. Whereas standardization has been proposed by the International Federation of Clinical Chemistry (IFCC) for specific proteins, few data are available concerning the actual transferability of the FT proteins, i.e. haptoglobin, apolipoprotein A1 and α2 macroglobulin. The aim of this study was to evaluate the analytical variability of the FT proteins. Design and methods:: During the FIBROPACA study, we evaluated 112 sera from patients with hepatitis C infection who underwent liver biopsy. We compared measurements of haptoglobin, apolipoprotein A1 and α2 macroglobulin by the autoanalyzers Immage® (Beckman-Coulter) and the FT reference BNProspec® (Dade-Behring). Results:: Optimal concordance was found for haptoglobin (correlation: y = 1.05× −0.09; correlation coefficient = 0.98). However, apolipoprotein A1 as determined with Immage® was globally 12% lower than with BNProspec® (correlation: y = 0.88× −0.05; correlation coefficient = 0.91) and α2 macroglobulin values were 40% greater with Immage® than with BNProspec® (correlation: y = 1.40× −0.46; correlation coefficient = 0.96). Conclusions:: Inter-technique analytical variability of the Fibrotest parameters remains a major issue. After IFCC standardization of specific proteins, some discrepancies remain for α2 macroglobulin and, to a lesser extent, for apolipoprotein A1. National and international quality control programs would be useful to monitor analytical performance of protein assays. [Copyright &y& Elsevier]