1. The use of lysozyme modified with fluorescein for the detection of Gram-positive bacteria.
- Author
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Arabski, Michał, Konieczna, Iwona, Tusińska, Ewa, Wąsik, Sławomir, Relich, Inga, Zając, Krzysztof, Kamiński, Zbigniew J., and Kaca, Wiesław
- Subjects
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LYSOZYMES , *GRAM-positive bacteria , *PHARMACEUTICAL industry , *SOIL microbiology , *FLUORIMETRY , *BIOREMEDIATION - Abstract
Lysozyme (1,4-β-N-acetylmuramidase) is commonly applied in the food, medical, and pharmaceutical industries. In this study, we tested a novel application of fluorescein-modified lysozyme (using carboxyfluorescein with a triazine-based coupling reagent) as a new tool for the detection of Gram-positive soil bacteria. The results, obtained by cultivation methods, fluorescence analysis, and laser interferometry, showed that, after optimization, fluorescein-modified lysozyme could be used to evaluate the prevalence of Gram-positive bacteria essential in bioremediation of soils with low pH, such as those degraded by sulfur. [ABSTRACT FROM AUTHOR]
- Published
- 2015
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