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Highlights: [•] A general method for isolating mutant strains with higher EPSs yield and viscosity. [•] Strains were exposed to Amp used as a stressor and mutagen during serial passage. [•] Further analysis indicated that high viscosity was related to high molecular weight. [Copyright &y& Elsevier]

Highlights: [•] Industrial yeast strains were engineered for more intercellular trehalose content. [•] The protective effect of trehalose depends on its content and the level of stress. [•] Trehalose accumulation is more important for VHG fermentation than regular one. [•] It had great potential for improving VHG performances of industrial yeast strains. [ABSTRACT FROM AUTHOR]

Abstract: The optimization, purification and characterization of bioflocculant produced by Paenibacillus elgii B69 were investigated. The bioflocculant was an exopolysaccharide composed of glucose, glucuronic acid, mannose and xylose. The maximum bioflocculant production was about 25.63g/L achieved with sucrose at 51.35g/L, peptone at 6.78g/L and yeast extract at 0.47g/L optimized by response-surface methodology. In addition, a series of experiments was performed to investigate the flocculation activities towards kaolin clay, dyeing pigment, heavy metal ion, and real wastewater and the result indicated the new bioflocculant had high activities towards all the tested pollutions. These results showed its great potential for water pretreatment used in industry. [Copyright &y& Elsevier]