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Abstract: We present investigation of nanocrystalline diamond films deposited in a wide temperature range. The nanocrystalline diamond films were grown on silicon and glass substrates from hydrogen based gas mixture (methane and hydrogen) by microwave plasma CVD process. Film composition, nano-grain size and surface morphology were investigated by Raman spectroscopy and scanning electron microscopy. All samples showed diamond characteristic line centred at 1332 cm−1 in the Raman spectrum. Nanocrystalline diamond layers revealed high surface flatness (under 10 nm) with crystal size below 60 nm. Surface morphology of grown films was well homogeneous over glass substrates due to used mechanical seeding procedure. Very thin films (40 nm) were successfully grown on glass slides (i.e. standard size 1×3″). An increase in delay time was observed when the substrate temperature was decreased. A possible origin for this behaviour was discussed. [Copyright &y& Elsevier]

Abstract: The adhesion, growth and viability of human osteoblast-like MG 63 cells and bovine pulmonary artery endothelial CPAE cells were investigated in cultures grown on nanocrystalline diamond (NCD) films with either low surface roughness (RMS of 8.2 nm) or hierarchically organized surfaces made of nano-dimensional roughness NCD films deposited on Si surfaces with the original microroughness (RMS of 301.1 nm and 7.6 nm, respectively). The number and viability of both cell types on NCD films was similar to the values obtained on control tissue culture polystyrene dishes, or even better. It can be concluded that nanocrystalline diamond films give good support for adhesion and growth of osteogenic and endothelial cells, and could be used in tissue engineering applications. [Copyright &y& Elsevier]