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The effect of shade on behavior and physiological attributes of grazing cows in a high altitude subtropical zone is not well established. This work aimed to investigate how social and ingestive behaviors, as well as physiological and other attributes of dairy cows such as milk production, change in a subtropical environment during the hot season either with or without free access to shade. Fourteen lactating cows were kept on pasture either with no shade or with free access to shade for 5 days and their behavior was recorded with instantaneous scan sampled every 10 min, from sunrise, 0530 h (Greenwich mean time, GMT−0200 h) to sunset, 2100 h (GMT−0200 h). Behavior traits included (1) time spent in activities such as grazing, ruminating, resting, lying, standing, walking, seeking shade and staying in the proximity to the water trough and (2) number of events such as water ingestion, aggressive interactions, as well as competition for shade and water. Physiological attributes such as heart and respiratory rates, rectal temperature, number of rumen movements, panting score, as well as milk yield, were evaluated. Time spent in behavioral activities, number of behavioral events and physiological attributes varied between groups (with and without access to shade). Cows with no shade showed increased respiratory and heart rates and panting score at 1300 h, higher values for time of permanence near the water trough, number of competition and aggression events for shade. On the other hand, they showed lower values for time spent resting while lying, ruminating while standing, seeking shade. Access to shade did not change time spent lying, standing, walking with the head up, ruminating while lying, resting while standing, as well as milk yield and number of ruminal movements. Significant interactions between access to shade and days of measurements were detected for time spent walking, ruminating, grazing, resting, number of water ingestion events, competition events near the water trough and for shade, as well as for rectal temperature and panting score measured at 1700 h. In the high altitude subtropical region, access to shade minimizes negative heat stress effects on behavior and physiological aspects of dairy cows. [ABSTRACT FROM PUBLISHER]

Thirty Aberdeen Angus crossbred steers (281±16 kg) were used to test the effect of finishing feeding system on growth performance, meat quality and fatty acid (FA) profile in intramuscular fat. Steers were fed in confinement (forage:concentrate ratio of 50 : 50; DM basis) or with different levels of energy supplementation (0, 0.4, 0.8 and 1.2% BW) at pasture (Avena strigosa Schreb and Lolium multiflorum L.). There were no differences between treatments for ADG (average=1.60 kg/day), hot carcass weight (HCW) (average=229 kg) and subcutaneous fat depth (average=3 mm). Dressing % (P=0.06; tendency) and carcass ADG (P=0.02) linearly increased with level of supplementation for pasture steers. No differences were observed between treatments for tenderness, marbling, pH, color b*, or cooking loss and drip loss in samples of Longissimus dorsi. However L* increased linearly (P=0.05) with level of supplementation. The concentrations of myristic, palmitic, estearic and linoleic FA did not differ among treatments. The concentration of n-3 FA increased (P<0.001) in steers at pasture compared with confinement, but n-6 FA concentrations did not differ between feeding system. Supplementation up to 0.4% BW increase (P<0.001) conjugated linoleic acid (CLA) and linolenic FA concentrations in intramuscular fat when compared with confinement. The level of supplementation on pasture linearly decreased (P<0.001) n-3 and CLA and linearly increased (P=0.001) the n-6 : n-3 ratio. Finishing of steers grazing winter pasture with energy supplementation or in confinement fed a medium-concentrate diet did not affect meat quality (tenderness, marbling, parameter b* on the CIE L*a*b* scale, cooking and drip losses) except for a* and L*. However, intramuscular fat of animals finished at pasture with moderate level of supplementation compared to animals fed in confinement had greater concentration of CLA, linolenic, and n-3, and lower n-6 : n-3 in intramuscular fat. [ABSTRACT FROM PUBLISHER]

Upper respiratory infection (URI) is a pervasive problem in cats and impacts the capacity and cost of sheltering programs. This study determined the pattern of respiratory pathogens in cats with and without clinical signs of URI in four different models for managing unowned cats, namely, (1) short-term animal shelters (STS), (2) long-term sanctuaries (LTS), (3) home-based foster care programs (FCP), and (4) trapneuter- return programs for community cats (TNR). Conjunctival and oropharyngeal swabs from 543 cats, approximately half of which showed clinical signs of URI, were tested for feline herpes virus-1 (FHV), feline calicivirus (FCV), Chlamydia felis, Bordetella bronchiseptica, Mycoplasma felis, and canine influenza virus by real-time PCR. FHV (59%, 41%) and B. bronchiseptica (33%, 24%) were more prevalent in both clinically affected and nonclinical cats, respectively, in STS than other management models. FCV (67%, 51%) and M. felis (84%, 86%) were more prevalent in LTS than any other management model. Clinically affected cats in FCP were more likely to carry FHV (23%, 6%), C. felis (24%, 10%), or M. felis (58%, 38%) than were nonclinical cats. Clinically affected cats in TNR were more likely to carry FCV (55%, 36%) or C. felis (23%, 4%) than were nonclinical cats. The prevalence of individual pathogens varied between different management models, but the majority of the cats in each model carried one or more respiratory pathogens regardless of clinical signs. Both confined and free-roaming cats are at risk of developing infectious respiratory disease and their health should be protected by strategic vaccination, appropriate antibiotic therapy, effective biosecurity, feline stress mitigation, and alternatives to high-density confinement. [ABSTRACT FROM AUTHOR]

A total of twelve lactating Jersey cows were used in a 5-week experiment to determine the effects of severe feed restriction on the permeability of mammary gland cell tight junctions (TJs) and its effects on milk stability to the alcohol test. During the first 2 weeks, cows were managed and fed together and received the same diet according to their nutritional requirements (full diet: 15 kg of sugar cane silage; 5.8 kg of alfalfa hay; 0.16 kg of mineral salt and 6.2 kg of concentrate). In the 3rd week, animals were distributed into two groups of six cows each. One group received the full diet and the other a restricted diet (50% of the full diet). In the 4th and 5th weeks, all animals received the full diet again. Milk composition and other attributes, such as titratable acidity, ethanol stability, pH, density and somatic cell count (SCC) were evaluated. Cortisol levels indicated the stress condition of the cows. Plasma lactose and milk sodium were measured to assess mammary TJ leakiness. Principal factor analysis (PFA) showed that the first two principal factors (PFs) contributed with 44.47% and 20.57% of the total variance in the experiment and, as feeding levels increased, milk stability to the ethanol test became higher and plasma lactose levels decreased, which indicates lower permeability of the mammary gland cell TJ. Correspondence analyses were consistent with PFA and also showed that lower feeding levels were related to reduced milk stability, high plasma lactose, high sodium in milk, low milk lactose (another parameter used to assess TJ permeability) and higher cortisol levels, indicating the stress to which animals were submitted. All observations were grouped in three clusters, with some of the above-mentioned patterns. Feeding restriction was associated with higher permeability of TJ, decreasing milk stability to the ethanol test. [ABSTRACT FROM PUBLISHER]

Summary: Genomic imprinting occurs when expression of an allele differs based on the sex of the parent that transmitted the allele. In D. melanogaster, imprinting can occur, but its impact on allelic expression genome-wide is unclear. Here, we search for imprinted genes in D. melanogaster using RNA-seq to compare allele-specific expression between pools of 7- to 10-day-old adult female progeny from reciprocal crosses. We identified 119 genes with allelic expression consistent with imprinting, and these genes showed significant clustering within the genome. Surprisingly, additional analysis of several of these genes showed that either genomic heterogeneity or high levels of intrinsic noise caused imprinting-like allelic expression. Consequently, our data provide no convincing evidence of imprinting for D. melanogaster genes in their native genomic context. Elucidating sources of false-positive signals for imprinting in allele-specific RNA-seq data, as done here, is critical given the growing popularity of this method for identifying imprinted genes. [ABSTRACT FROM AUTHOR]

Alternative splicing is a widespread means of increasing protein diversity and regulating gene expression in eukaryotes. Much progress has been made in understanding the proteins involved in regulating alternative splicing, the sequences they bind to, and how these interactions lead to changes in splicing patterns. However, several recent studies have identified other players involved in regulating alternative splicing. A major theme emerging from these studies is that RNA secondary structures play an under appreciated role in the regulation of alternative splicing. This review provides an overview of the basic aspects of splicing regulation and highlights recent progress in understanding the role of RNA secondary structure in this process. [ABSTRACT FROM AUTHOR]

This paper describes a collaborative project that joins academic perspectives and clinical practice perspectives in a focused clinical experience. This activity involves 3rd quarter associate degree nursing students in a medical-surgical course. Instructors identified the need to provide an activity to develop intravenous therapy skills and provide increased opportunities, thus increasing technical skill and assessment skills. This led to the development of the focused clinical experience. During this clinical rotation, the students rotate through either a Same Day Surgery unit or an endoscopy unit for one of their clinical days. The primary objective of this clinical day is to provide intravenous therapy. The duration of this project was a 10-week quarter of the curriculum and involved 38 students in the 2002 class and 30 in the 2003 class. Results indicate a very positive learning experience for the student, as well as a collaborative and contributing effort by the students for the staff. The staff, on the other hand, assists in providing a mentoring role in shared expertise. Collaborative partnerships between academia and practice can help bridge the gap by providing opportunities to share perceptions, creating an environment for shared knowledge, and networking opportunities, leading to further enabling preparation for potentially future staff. [ABSTRACT FROM AUTHOR]

Abstract: The aim of this study was to typify competitiveness on beef cattle farms from the western border region of the state of Rio Grande do Sul, Brazil. Sixty-three farmers, each with an individual farm area exceeding 900ha, were interviewed using a semi-structured questionnaire, divided in four sections: technology (TEC), management (MAN), market relationships (MR) and institutional environment (IE). Data were analysed using Cluster and Discriminant analyses. Beef cattle producers were divided into three levels of competitiveness: low (LCL), medium (MCL) and high (HCL). Comparing LCL×MCL, the former group of farmers showed lower levels of pasture and reproduction management than the latter (subfactors within TEC). When LCL×HCL were compared, the main differences were the lower access to technological innovation and low investment with herd genetics of LCL compared with HCL. The lower level of management activities (performance recording, animal handling and calculation of financial parameters) of MCL compared with HCL were the main variables that differ between these farms. Cattle producers interviewed here were, in general, competitive, mainly due to the use of technologies on farm. However, there were limitations in the variables related to management. [Copyright &y& Elsevier]

Abstract: Studies were conducted to characterize the effect of season of the year on testicular morphology, fresh and frozen/thawed semen quality from Curraleiro (Pé-duro) bulls in the Brazilian Central west region. Five adult, healthy bulls underwent an andrological examination and semen collection using an electroejaculator, once a month for a year. Fresh and thawed semen were evaluated for progressive sperm motility and sperm vigor, sperm morphology and acrosomal integrity. Testicular length and volume were less (P <0.05) in April than in the other months of the year. For fresh semen, the ejaculate in April had less volume and sperm concentration (P <0.05), while sperm vigor was less (P <0.05) in June, increasing in January and February. With the frozen/thawed semen, the proportion of sperm was greater (P <0.05) in April to July, decreasing from October to December. Semen collected in December had the greatest (P <0.05) proportion of major defects while that collected in February/March had the highest proportion of minor defects. The proportion of live intact sperm reduced progressively from December to April/May. The marginal influence of the time of the year on testicular biometry and fresh semen in Curraleiro bulls shows the adaptation of this breed to the environmental conditions in the region. Thus, reproduction with natural mating should be successful at any time of year. For frozen semen collection for conservation programs, the best time of year is from June to September. [Copyright &y& Elsevier]

Strategies for the sustainable intensification of ruminant production suggest breeding animals adapted to the environment in which they live using local fodders to maximize efficient ruminal fermentation. The purpose of this study was to explore the effects of using Orbignya phalerata (Babassu) and Combretum leprosum (Mofumbo) leaves in ruminal short chain fatty acids (SCFA) and microbial community, as well as finishing performance, carcass characteristics and meat fatty acid profile of Santa Inês hair lambs. The experimental treatments were diets with 50:50 forage:concentrate ratios, using the leaves of the experimental plants as a 33 g/100 g of dry matter (DM) replacement of Cynodon dactylon (Tifton-85) hay, with three groups: Control (no hay replacement), Babassu and Mofumbo. Twenty-four Santa Inês lambs (body weight = 20.0 ± 5.2 kg) were used in a randomized experimental design with eight repetitions (5 males and 3 female) per treatment. Ruminal fluid samples of each animal were collected to determine SCFA and microbial community. The male animals were evaluated for finishing performance and carcass characteristics while longissimus lumborum muscle samples were used for determination of fatty acid profile. Interaction between treatment and sex was observed for total SCFA ( P < 0.05). Treatment affected ( P < 0.05) propionate, isobutyric, isovaleric and valeric fatty acids. Mofumbo showed a greater relative abundance of rumen fungi and Ruminococcus flavefaciens , and lower Archaea. There was no difference between treatments ( P > 0.05) for finishing performance and carcass characteristics but meat fatty acid characteristics were affected by treatments. A redundancy analysis based on microbial abundance profile demonstrated two clusters, one cluster with Babassu and Control treatments, and other cluster with Mofumbo, with some variables associated to dissimilarity between clusters. These results indicated that the inclusion of these plants in lamb diets affects ruminal short chain fatty acids and microbial population, without compromising the production potential, carcass characteristics and meat fatty acid profile. [ABSTRACT FROM AUTHOR]

The objective of this study was to evaluate the heat tolerance of five zebu breeds using physical, physiological and hematological traits as well as thermographic responses. Forty cows of the Gir, Girolando, Nelore, Sindhi and Indubrasil breeds (eight cows each), approximately three years of age, were evaluated. Body weight, withers and hump heights as well as thoracic circumference were recorded. The density and length of the hair was obtained by collecting one square centimeter in the rump region and skin color using the CIELAB system. Rectal temperature, heart and respiratory rates were evaluated during the morning at 4:30 h, and in the afternoon, at 14:30 h, with six repetitions. Blood samples were collected for hematological evaluation. The surface temperature was obtained using an infrared camera FLIR ® T400. Two images were taken from each animal, one laterally of the whole body and the other of the head region. Air temperature, wind speed, relative humidity were obtained from a mobile weather station. The statistics analysis included an analyzes of variance, principal factors, as well as cluster, discriminant and canonical analyzes, logistic regression and calculation of odds ratio. There were significant differences in the rectal temperature, heart and respiratory rates between breeds. Gir and Indubrasil breeds had the highest rectal temperatures. Breed was significant for surface temperatures and showed that physical and physiological factors affected breeds in different ways. Eye and brain surface temperatures were the most affected by environmental parameters. Also, environmental parameters affected packed cell volume and red cell number. Odds ratio test showed that the Gir breed was three times more likely to have higher rectal temperature compared with Sindhi as confirmed by the logistic regression. When the black globe temperature approached 35 °C, the probability of the Gir animals having rectal temperatures above normal was approximately 70%. Gir was the breed least adapted to climate conditions of the experiment while the Sindhi and Girolando breeds showed the best physiological response to thermal stress. [ABSTRACT FROM AUTHOR]

Heat is a major factor limiting the production of animals in the tropics. Sheep are well adapted to diverse ecosystems, however, temperature and relative humidity can influence animal husbandry. Therefore, this study was carried out to verify the thermoregulation in rams of six breeds (Bergamasca, Dorper, Ile de France, Hampshire Down, Santa Ines and Texel) through the evaluation of physiological traits, body measures, laser and thermographic infrared temperatures as well as testicle morphometry associated with heat tolerance. Animals were measured and weighed, coat and hair colour determined, as well as physiological traits and laser and thermographic infrared temperatures measured twice a day. Data were analyzed with the Statistical Analysis System ® . There were differences among breeds for most of the traits linked to heat tolerance, with the Santa Ines showing better adaptation to heat stress compared to other breeds. The Dorper was not significantly better than Bergamasca or Hampshire Down breeds, while Texel and Ile de France were less well adapted. Scrotal temperatures were lowest in Hampshire Down and Dorper, while highest in Texel. Temperature gradient measured by thermography identified Hampshire Down as the least heat tolerant breed. Traits linked to heat tolerance should be taken into consideration when choosing breeds for lamb production in tropical regions. [ABSTRACT FROM AUTHOR]

To evaluate the effect of heat stress on sperm characteristics, as well as testicular biometrics and heat radiation, six breeds of rams: two Brazilian locally adapted breeds (Santa Ines and Bergamasca) and four exotic breeds (Dorper, Texel, Ile de France, and Hampshire Down) underwent scrotal insulation. The experiment consisted of pre-scrotal insulation (week −1), insulation (week 0), and post-insulation (week 1–11) phases. Insulation changed the scrotum and testicular measurements, the heat radiated from the gonads caused deleterious effects, both on the process of spermatogenesis and sperm maturation in the epididymis. However, all the characteristics studied showed reversibility at 11 weeks post-insulation, showing the ability of the seminiferous epithelium to react to the effects of environmental factors. The locally, adapted breeds were more resistant than exotic to testicular heat stress. [ABSTRACT FROM AUTHOR]

Abstract: The aim of this work was to evaluate the growth performance, carcass traits, hematological and biochemical variables of Santa Inês lambs fed diet supplemented with physic nut (Jatropha curcas) meal (PNM). Twenty-four intact male lambs [age: 120±0.98 days; body weight (BW): 19.7±2.4kg] were assigned randomly to four concentrate mixtures. These mixtures were supplemented with 0 (CON), 100 (PNM100), 200 (PNM200), or 300 (PNM300)g/kg dry matter (DM) of PNM. The concentrate was offered at 1.2% BW, whereas lambs were fed Tifton (Cyndon dactylon CV. Tifton-85) hay ad libitum. Animals were weighed and blood collected every 15 days. At the end of the experiment, lambs were slaughtered and their carcasses were evaluated. Biochemical and hematological variables were not different among diets (P >0.05). Similarly, dry matter intake was not different among diets (P >0.05) and no acceptability problems were observed for the PNM. Average daily gain and carcass characteristics also did not differ (P >0.05) between the treatments. No clinical symptoms of intoxication by physic nut were detected during the whole experimental period. Therefore, it may be inferred that the non-toxic PNM was shown to be a promising protein source for sheep nutrition and can be used in concentrations of up to 300g/kg DM in concentrate mixture. [Copyright &y& Elsevier]

Abstract: Fifteen morphometric traits and body weight (BW) from 319 adult Crioula Lanada ewes were evaluated. The influence of wool type and geographic location (fixed effects) was tested, in addition to Pearson correlation, factor and discriminatory analysis according to geographic regions and breed ecotypes. Analysis was carried out using the Statistical Analysis System Program. Geographic location revealed significant effect (P <0.001) on morphometric traits. Most correlations were significant (P <0.05), thoracic perimeter (TP) with BW (0.72) being the strongest. Correlations between head and body measurements were medium or weak, strongest being between BWs. Multivariate analysis revealed 81% of variance using two factors, where three groups of variables were identified (general body, head and one group including thoracic and BW, respectively). Fine discrimination between herds and also the Fronteira and Serrana ecotypes were observed which opens door to valuable validation of how phenotype relates to genotype and associated SNPs in the Crioula Lanada breed. [Copyright &y& Elsevier]

The article presents an abstract of the research paper "Correlation and principal component analyses of corn grain from Brazilian Cerrado region," by S.I.F. Rodrigues and colleagues.

The article presents an abstract of the research paper "Physical and chemical analysis of corn grain from Brazilian Cerrado Region," by S.I.F. Rodrigues.

Abstract: Three genetic groups of sheep (Santa Ines – SI) and their crosses with Texel (TxSI) and Ile de France (IxSI) were tested for heat tolerance in the center-west region of Brazil. Lambs were evaluated twice a day (in the morning with no heat stress and in the afternoon under stressful conditions). As well as physiological traits (heart rate, respiratory rate, rectal temperature, and sweating rate), animals were measured for size (height, weight, length, and thoracic circumference), blood parameters (red cell and white cell counts), coat colour and thermal indices were calculated. Crossbred animals were longer, taller and were growing faster than the SI. The Texel cross had more animals with rectal temperature above the reference values when compared to the other groups. Animal size measures did not affect most of the traits examined. In terms of blood parameters, the TxSI were more susceptible with higher reactions to heat stress. Most correlations of TR with adaptive skin traits (such as skin temperature and sweating rate) and blood traits were low. Animals with less thermal insulation used less physiological resources to dissipate heat. Texel×Santa Ines animals were, in general, less well adapted than the other genetic groups. [Copyright &y& Elsevier]

Abstract: Several naturalized sheep breeds in the South American continent have little objective information available and as population size is becoming smaller the need for this information becomes more critical. The objective of this study was to differentiate between naturalized breeds in Brazil, Uruguay and Colombia using morphological descriptors and compare them with commercial breeds. The amount of 928 morphometric measurements and 2918 weights were collected. Phenotypic characterization was carried out using size, weight, colour and conformation of the animals, as well as 16 morphometric measurements of the animals. Phenotypic data were analysed using PROC GLM, CORR and PRINCOMP of Statistical Analysis System (SAS®). The distance between breeds was carried out using morphometric and morphological data using UPGMA (Unweighted Pair Group Method Arithmetic Mean) method to create a dendrogram. Phenotypic characterization was shown to be an accessible and easy to use tool in conservation and breeding programs. The breed was the most important factor to differentiate between the animals measured, and adult weight most influenced by the environment. Commercial breeds were larger and Santa Inês animals from the Center-west and Southwest closer to Bergamasca than in other regions. [Copyright &y& Elsevier]

The aims of this study were to describe the pattern of excess winter mortality and emergency hospital admissions in the South Yorkshire Coalfields Health Action Zone, and to examine the relationship between excess winter mortality and emergency hospital admissions and socio-economic deprivation at the enumeration district level. We analysed monthly deaths from 1981 to 1999 and monthly emergency hospital admissions from 1990 to 1999 for cardiovascular disease, respiratory disease and all other causes of death for people aged 45 years and above. We used the enumeration district level Townsend socio-economic deprivation score to categorize enumeration districts by quintile. Excess winter mortality ratios (observed/expected) for females and males, respectively, were 1.70 and 1.58 for respiratory disease, 1.25 and 1.20 for cardiovascular disease, and 1.09 and 1.07 for all other causes of death. The excess winter hospital admission ratio for respiratory disease was 1.80 for females and 1.58 for males. No excess was evident for the other two groups of conditions. We found no significant increase in excess winter mortality ratios with increasing socio-economic deprivation. There was also no significant increase in the excess winter respiratory admission ratio with increasing deprivation. With regard to age, we found significant increases with increasing age in the excess winter mortality ratios for cardiovascular disease (P < 0.0001) and for all other diseases (P < 0.001), and also in the excess winter hospital admission ratio for respiratory disease (P < 0.0001). With regard to sex, the excess ratios were lower in men than in women for both respiratory mortality (P < 0.05) and respiratory hospital admissions (P < 0.0001). We also observed that excess winter mortality ratios decreased significantly over the 18-year period for cardiovascular disease (P < 0.05) and for all other diseases (P < 0.05). Our results suggest that measures to reduce excess winter mortality should be implemented on a population-wide basis and not limited to socio-economically deprived areas. There may also be a case for tailoring interventions to specifically meet the needs of older people. [ABSTRACT FROM AUTHOR]