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• Circ_0005576 remarkedly increases in cervical cancer and most cell lines. • Circ_0005576 expression significantly correlates with the clinical parameters and poor prognosis in cervical cancer. • Circ_0005576 knockdown inhibits cervical cancer cell proliferation and mobility. • Circ_0005576 promotes cell mobility and proliferation through mir-153-3p/KIF20A axis. Circular RNAs (circRNAs) are a novel group of noncoding RNAs characterized by a covalently closed loop. An increasing evidence suggests that deregulated circRNAs exert their essential regulatory roles in oncogenesis. However, little is explored on the biological role of novel circRNAs in cervical cancer (CC) progression. In the present study, we analyzed two GSE microarrays to screen for CC-specific circRNAs and found two circRNAs both expressed in CC cells and tissues. Among them, circ_0005576 was significantly overexpressed in both CC tissues and cell lines. Furthermore, upregulated circ_0005576 was positively associated with advanced FIGO stage, lymph node metastasis, but was negatively related with overall survival of CC patients. Additionally, circ_0005576 knockdown induced a suppressed cell growth, colony formation and metastasis of HeLa and SiHa cells. Mechanistically, circ 0005576 was mainly located in the cytoplasm and served as a sponge of miR-153-3p to increase kinesin family member 20A (KIF20A) expression. Rescue assays further validated the effects of circ_0005576/miR-153-3p/KIF20A axis on CC proliferation, migration and invasion. In conclusion, our research reveals a novel circ_0005576/miR-153-3p/KIF20A axis promoting CC progression, which may suggest a new insight into the pathogenesis of CC. [ABSTRACT FROM AUTHOR]

Porcine epidemic diarrhea (PED) is a serious disease requiring a simple and accurate detection method. Accordingly, this study developed a novel, ultrasensitive photoelectrochemical (PEC) sensing platform using the loop-mediated isothermal amplification (LAMP) technique (LAMP-PEC). An amino (−NH 2)-modified LAMP product is obtained by amplification of the PED virus gene with specially designed primers. The generated NH 2 -modified LAMP product is assembled on the surface of an electrode by forming imine linkages between aldehyde and amino groups based on the Schiff base reaction. A stable photocurrent is provided by a CdIn 2 S 4 photoactive material, which possesses high photoelectric conversion efficiency. Amplified DNA assembled on the electrode surface increases steric hindrance and hinders electrons from moving from the electrode to electron acceptors, which decreases the photocurrent. This strategy can detect PEDV with a low detection limit of 0.3 fg μL−1 and a wide linear range of 1 × 10−3-1 × 102 pg/μL. The sensing platform has excellent specificity and sensitivity and can be used for the quantitative detection of many other pathogens with the assistance of LAMP. [Display omitted] • LAMP-PEC sensing platform was combined the LAMP technique with PEC sensing. • The photochemical performance provided a wide detection range for PEDV detection. • LAMP-PEC sensing platform possessed high sensitivity, specificity and accuracy. [ABSTRACT FROM AUTHOR]

[Display omitted] • The Ni 5 P 4 nanosheets exhibit crystalline@amorphous core–shell structure. • The amorphous shell renders the CA-Ni 5 P 4 @NiO x /NF electrode superhydrophilic. • CA-Ni 5 P 4 @NiO x /NF exhibits excellent activity for both UOR and HER. • DUFC with CA-Ni 5 P 4 @NiO x /NF as anode could reach as power output of 3.4 mW/cm2. The electrochemical urea oxidation (UOR) involves the generation of gases bubbles, which often cause the blockage of the electrode surface leading to decline activity. Herein, we report the growth of Ni 5 P 4 nanosheets on nickel foam as bifunctional electrode (CA-Ni 5 P 4 @NiO x /NF) for urea electrolysis. The results indicated that a thin layer of amorphous NiOx formed on the surface of Ni 5 P 4 nanosheets to yield a unique crystalline@amorphous core–shell structure, which can be maintained during long time urea electrolysis. The outstanding UOR performance of CA-Ni 5 P 4 @NiO x /NF was attributed to the unique structure, which not only combine the good conductivity and abundant active sites, but also rendered the CA-Ni 5 P 4 @NiO x /NF superhydrophilic and aerophobic that significantly facilities the release of gases bubbles formed during UOR to avoid the blockage of the active sites. As a result, the CA-Ni 5 P 4 @NiO x /NF electrode exhibited excellent performance for UOR, which only needs 1.45 V (vs. RHE) to deliver a current of 100 mA/cm2. When used as anode for direct urea fuel cell (DUFC), it could reach a maximal power density as high as 3.4 mW/cm2 with an OCV of 0.76 V. The CA-Ni 5 P 4 @NiO x /NF also exhibited excellent activity for hydrogen evolution reaction (HER), with -0.089 V (vs. RHE) to deliver a current of 10 mA/cm2. The electrolyzer constructed with CA-Ni 5 P 4 @NiO x as both anode and cathode could run for more than 10 h at a high current load about 100 mA/cm2 without appreciable potential change, indicating the superb stability of the CA-Ni 5 P 4 @NiO x electrode. Our work provides new insights for understanding as well as the development of advanced electrode for DUFC and urea electrolysis. [ABSTRACT FROM AUTHOR]

Poly(1,2,3-triazolium) (PTAM) is a kind of self-healing adhesive based on dynamic reversible covalent 1,2,3-triazolium, which is formed through the reaction of halogenated hydrocarbon and 1,2,3-triazole and plays an important role in the properties of PTAM. Therefore, it is valuable to understand the effect of halogenated hydrocarbon crosslinkers on the properties of PTAM. A series of PTAM adhesive with different crosslinkers have been prepared. The reactivity between halogenated hydrocarbon and 1,2,3-triazole was different. Crosslinkers with longer chain, iodine substituted and aromatic structure improved the adhesive property of PTAM adhesive joints, which was up to 17 MPa. The first self-healing efficiency of PTAM adhesive joints with aliphatic bromine crosslinkers reached more than 100% and the maximum cycle times of them was more than 9 before failure. The self-healing properties of PTAMs depend on the segment motion and anti-thermal oxygen aging of PTAMs. These results provide some insights for the structural design of self-healing adhesive and dynamic covalent materials. [Display omitted] • A series of self-healing Poly(1,2,3-triazolium) (PTAM) adhesives are obtained. • Long aliphatic, iodine-substituted and aromatic halohydrocarbon enhance adhesion. • PTAM with aliphatic bromine-substituted halohydrocarbon have better self-healing. • The first self-healing efficiency of PTAM reach more than 100%. [ABSTRACT FROM AUTHOR]