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Your search keyword '"Lopes, Cassiane Elisabete"' showing total 2 results
Start Over Author "Lopes, Cassiane Elisabete" Publisher elsevier b.v.
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2. Frozen bovine preputial mucus as a suitable sample for the direct molecular diagnosis of Campylobacter fetus subsp. venerealis.

Author

De Carli, Silvia, Saggin, Bianca, Dias, Maria Eduarda, Lopes, Cassiane Elisabete, Dalto, André Gustavo Cabrera, Rolim, Veronica Machado, Zucco, Renato, Pastre, Giovani, Borges, João Baptista, and Siqueira, Franciele Maboni

Subjects
*MOLECULAR diagnosis, *CAMPYLOBACTER, *MUCUS, *BOS, *FETUS, *FREEZING
Abstract

Long transportation times remain a challenge to the satisfactory diagnosis of Campylobacter fetus subsp. venerealis (Cfv). Here we demonstrated that samples of frozen bovine preputial mucus maintained at −20 °C for 10 days can be used as an alternative source for molecular diagnosis of Cfv. This approach will improve the analysis of this bacterium. • Cfv molecular diagnosis with frozen preputial mucus is similar to unfrozen samples. • Frozen preputial mucus is a good alternative to molecular diagnosis of BGC. • DNA from preputial mucus remains satisfactory to PCR assay after freezing. • Frozen sample protocol should improve BGC detection. [ABSTRACT FROM AUTHOR]

Published
2020
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