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Monomethyl-branched chain fatty acids (mmBCFAs) are prevalent in diverse food sources, garnering significant interest owing to their distinctive branched structure and biological activity. However, the conventional analytical methods suffer from the time consumption and lower accuracy. In this study, we developed a method named Auto-mmBCFAs integrated GC-MS and algorithms for rapid and accurate measuring mmBCFAs. This method utilizes a multivariate matching approach, significantly enhancing detection efficiency. Compared to NIST database, Auto-mmBCFAs exhibits higher detection rates and accuracy. The developed method was validated in terms of precision, sensitivity, and linearity, and further evaluated mmBCFAs profile in sheep milk from different regions and breeds. Our results demonstrated that Auto-mmBCFAs methods is a rapid and reliable method for the determination of mmBCFAs in complex biological matrices. [Display omitted] [ABSTRACT FROM AUTHOR]

Purification of triglycerides from fully hydrogenated palm kernel oil (FHPKO) and fully hydrogenated coconut oil (FHCNO) was performed by a chromatographic method. Lipid composition, thermal properties, polymorphism, isothermal crystallization behaviour, nanostructure and microstructure of FHPKO, FHPKO-triacylglycerol (TAG), FHCNO and FHCNO-TAG were evaluated. Removal of minor components had no effect on triglycerides composition. However, the presence of the minor components did increase the slip melting point and promote onset of crystallization. Furthermore, the thickness of the nanoscale crystals increased, and polymorphic transformation from β′ to β occurred in FHPKO after the removal of minor components, and from α to β′ in FHCNO. Sharp changes in the values of the Avrami constant K and exponent n suggested that the presence of minor components changed the crystal growth mechanism. The PLM results indicated that a coarser crystal structure with lower fractal dimension appeared after the removal of minor components from both FHPKO and FHCNO. [ABSTRACT FROM AUTHOR]

• Epoxy group weakened the digestion capacity of triglyceride. • Epoxy group increased the mean particle diameter of triglyceride. • Epoxy group decreased binding capacity of triglycerides to salt ions in digestive juice. • Epoxy group enhanced the flexibility of lipase. The digestion behavior of epoxy triglyceride, the main cytotoxic product of deep-frying oil, remains unknown, which may affect its biosafety. In this study, epoxy triglyceride (EGT) and triglyceride (GT) were used to reveal the effect of epoxy group on digestion. Digestibility rate analysis showed that the free fatty acids release rate of EGT was slower. To clarify this phenomenon, binding ability with salt ions in digestive juice and particle size were also been studied. Cluster size analysis indicated that epoxy group increased triglyceride particle size, resulting in smaller contact area between EGT and lipase. Interface behaviors displayed EGT decreased binding ability with salt ions in digestive juice. Spectroscopic analysis showed EGT caused the red shift of lipase peak, indicating that epoxy group changed lipase structure. Molecular dynamics simulation suggested EGT leads to loosen lipase structure. In conclusion, this study highlights that epoxy group could weaken the triglyceride digestion. [ABSTRACT FROM AUTHOR]

The molar heat capacities of 1-(2-hydroxy-3-chloropropyl)-2-methyl-5-nitroimidazole (Ornidazole) (C7H10ClN3O3) with purity of 99.72 mol% were measured with an adiabatic calorimeter in the temperature range between 79 and 380 K. The melting-point temperature, molar enthalpy, ΔfusHm, and entropy, ΔfusSm, of fusion of this compound were determined to be 358.59±0.04 K, 21.38±0.02 kJ mol−1 and 59.61±0.05 J K−1 mol−1, respectively, from fractional melting experiments. The thermodynamic function data relative to the reference temperature (298.15 K) were calculated based on the heat capacities measurements in the temperature range from 80 to 380 K. The thermal stability of the compound was further investigated by DSC and TG. From the DSC curve an intensive exothermic peak assigned to the thermal decomposition of the compound was observed in the range of 445–590 K with the peak temperature of 505 K. Subsequently, a slow exothermic effect appears when the temperature is higher than 590 K, which is probably due to the further decomposition of the compound. The TG curve indicates the mass loss of the sample starts at about 440 K, which corresponds to the decomposition of the sample. [Copyright &y& Elsevier]