7 results on '"Krippner-Heidenreich, Anja"'
Search Results
2. TNF-α–induced protein 3 (TNFAIP3)/A20 acts as a master switch in TNF-α blockade–driven IL-17A expression.
- Author
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Urbano, Paulo C.M., Aguirre-Gamboa, Raúl, Ashikov, Angel, van Heeswijk, Bennie, Krippner-Heidenreich, Anja, Tijssen, Henk, Li, Yang, Azevedo, Valderilio F., Smits, Lisa J.T., Hoentjen, Frank, Joosten, Irma, and Koenen, Hans J.P.M.
- Abstract
Background Anti-TNF inhibitors successfully improve the quality of life of patients with inflammatory disease. Unfortunately, not all patients respond to anti-TNF therapy, and some patients show paradoxical immune side effects, which are poorly understood. Surprisingly, anti-TNF agents were shown to promote IL-17A production with as yet unknown clinical implications. Objective We sought to investigate the molecular mechanism underlying anti-TNF–driven IL-17A expression and the clinical implications of this phenomenon. Methods Fluorescence-activated cell sorting, RNA sequencing, quantitative real-time PCR, Western blotting, small interfering RNA interference, and kinase inhibitors were used to study the molecular mechanisms in isolated human CD4 + T cells from healthy donors. The clinical implication was studied in blood samples of patients with inflammatory bowel disease (IBD) receiving anti-TNF therapy. Results Here we show that anti-TNF treatment results in inhibition of the anti-inflammatory molecule TNF-α–induced protein 3 (TNFAIP3) /A20 in memory CD4 + T cells. We found an inverse relationship between TNFAIP3 /A20 expression levels and IL-17A production. Inhibition of TNFAIP3 /A20 promotes kinase activity of p38 mitogen-activated protein kinase and protein kinase C, which drives IL-17A expression. Regulation of TNFAIP3 /A20 expression and cognate IL-17A production in T cells are specifically mediated through TNF receptor 2 signaling. Ex vivo , in patients with IBD treated with anti-TNF, we found further evidence for an inverse relationship between TNFAIP3 /A20 expression levels and IL-17A–producing T cells. Conclusion Anti-TNF treatment interferes in the TNFAIP3 /A20-mediated anti-inflammatory feedback loop in CD4 + T cells and promotes kinase activity. This puts TNFAIP3 /A20, combined with IL-17A expression, on the map as a potential tool for predicting therapy responsiveness or side effects of anti-TNF therapy. Moreover, it provides novel targets related to TNFAIP3 /A20 activity for superior therapeutic regimens in patients with IBD. [ABSTRACT FROM AUTHOR]
- Published
- 2018
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3. Single chain TNF derivatives with individually mutated receptor binding sites reveal differential stoichiometry of ligand receptor complex formation for TNFR1 and TNFR2
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Boschert, Verena, Krippner-Heidenreich, Anja, Branschädel, Marcus, Tepperink, Jessica, Aird, Andrew, and Scheurich, Peter
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TUMOR necrosis factor receptors , *BINDING sites , *STOICHIOMETRY , *LIGAND binding (Biochemistry) , *MONOCLONAL antibodies , *RECEPTOR-ligand complexes - Abstract
Abstract: Most members of the tumor necrosis factor ligand family form noncovalently linked homotrimers, capable to bind up to three molecules of the respective membrane receptors. For several receptors a membrane distal homophilic interaction domain has been identified, called pre-ligand binding assembly domain. Accordingly, affinity values determined by typical equilibrium binding studies are likely to be influenced by avidity effects. Using our recently introduced covalently stabilized TNF (single chain TNF, scTNF), we have here investigated receptor–ligand binding stoichiometry in our well characterized system of TNFR–Fas chimeras. We produced scTNF derivatives with functionally deleted individual receptor binding sites, resulting in TNF mutants capable to only bind to one or two receptor molecules, rather than three. Equilibrium binding affinity studies on ice with these molecules revealed no significant changes after a single receptor binding site had been functionally deleted. In contrast, functional abrogation of two receptor binding sites showed a strong decrease in both, affinity and bioactivity on TNFR2–Fas. In contrast, TNFR1–Fas ligand binding and receptor activation was only affected after functional deletion of all three receptor binding sites. Our data demonstrate pivotal differences in ligand/receptor interactions between TNFR1–Fas and TNFR2–Fas, arguing for avidity effects important for TNF binding and downstream signaling of TNFR2, but to a lesser extent of TNFR1. These results are supported by data revealed from chemical crosslinking experiments suggesting the existence of preformed TNFR–Fas homodimers. [Copyright &y& Elsevier]
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- 2010
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4. Fluorescence correlation spectroscopy reveals topological segregation of the two tumor necrosis factor membrane receptors
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Gerken, Margarita, Krippner-Heidenreich, Anja, Steinert, Steffen, Willi, Sylvia, Neugart, Felix, Zappe, Andrea, Wrachtrup, Jörg, Tietz, Carsten, and Scheurich, Peter
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TUMOR necrosis factors , *CELL receptors , *FLUORESCENCE spectroscopy , *CYTOKINES , *GREEN fluorescent protein , *LIGAND binding (Biochemistry) , *CELL membranes - Abstract
Abstract: The proinflammatory cytokine tumor necrosis factor (TNF) binds two distinct plasma membrane receptors, TNFR1 and TNFR2. We have produced different receptor mutants fused with enhanced green fluorescent protein to study their membrane dynamics by fluorescence correlation spectroscopy (FCS). TNFR1 mutants show diffusion constants of approximately 1.2×10−9 cm2/s and a broad distribution of diffusion times, which is hardly affected by ligand binding. However, cholesterol depletion enhances their diffusion, suggesting a constitutive affinity to cholesterol rich membrane microdomains. In contrast, TNFR2 and mutants thereof diffuse rather fast (D̄ =3.1×10−9 cm2/s) with a marked reduction after 30min of TNF treatment (D̄ =0.9×10−9 cm2/s). This reduction cannot be explained by the formation of higher ordered receptor clusters, since the fluorescence intensity of TNF treated receptors indicate the presence of a few receptor molecules per complex only. Together, these data point to a topological segregation of the two TNF receptors in different microcompartments of the plasma membrane independent of the cytoplasmic signaling domains of the receptors. [Copyright &y& Elsevier]
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- 2010
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5. The Met-196 → Arg Variation of Human Tumor Necrosis Factor Receptor 2 (TNFR2) Affects TNF-α-induced Apoptosis by Impaired NF-κB Signaling and Target Gene Expression.
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Till, Andreas, Rosenstiel, Philip, Krippner-Heidenreich, Anja, Mascheretti-Croucher, Silvia, Croucher, Peter J. P., Schäfer, Heiner, Scheurich, Peter, Seegert, Dirk, and Schreiber, Stefan
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TUMOR necrosis factors , *APOPTOSIS , *NF-kappa B , *GENE expression , *MACROPHAGES , *GLYCOPROTEINS - Abstract
Tumor necrosis factor-α (TNF-α)-induced signaling is pivotally involved in the pathogenesis of chronic inflammatory diseases. A polymorphism in the TNF receptor 2 (TNFR2) gene resulting in a juxtamembrane inversion from methionine (TNFR2196MET) to arginine (TNFR2196ARG) has been genetically associated with an increased risk for systemic lupus erythematosus and familial rheumatoid arthritis. Albeit the mutation does not affect the TNF binding kinetics of TNFR2, the present study provides evidence that the mutation results in a significantly lower capability to induce TNFR2mediated NF-κB activation. Pretriggering of TNFR2 with a receptor-specific mutein leads to an enhancement of TNFR1-induced apoptosis, which is further increased in cells carrying the TNFR2196ARG variant. A diminished induction of NF-κB-dependent target genes conveying either anti-apoptotic or pro-inflammatory functions, such as cIAP1, TRAF1, IL-6, or IL-8 is observed. The mutated form TNFR2196ARG shows a reduction of inducible TRAF2 recruitment upon TNF-α stimulation. The findings suggest a common molecular mechanism for the involvement of the TNFR2196ARG variant in the etiopathogenesis of different chronic inflammatory disorders. [ABSTRACT FROM AUTHOR]
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- 2005
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6. Ligand-induced internalization of TNF receptor 2 mediated by a di-leucin motif is dispensable for activation of the NFκB pathway
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Fischer, Roman, Maier, Olaf, Naumer, Matthias, Krippner-Heidenreich, Anja, Scheurich, Peter, and Pfizenmaier, Klaus
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ANTIGENS , *EPIDERMAL growth factor , *GREEN fluorescent protein , *POLYOXYMETHYLENE , *TUMOR necrosis factor receptors , *ENDOCYTOSIS , *LYSOSOMES - Abstract
Abstract: Endocytosis is an important mechanism to regulate tumor necrosis factor (TNF) signaling. In contrast to TNF receptor 1 (TNFR1; CD120a), the relevance of receptor internalization for signaling as well as the fate and route of internalized TNF receptor 2 (TNFR2; CD120b) is poorly understood. To analyze the dynamics of TNFR2 signaling and turnover at the plasma membrane we established a human TNFR2 expressing mouse embryonic fibroblast cell line in a TNFR1−/−/TNFR2−/− background. TNF stimulation resulted in a decrease of constitutive TNFR2 ectodomain shedding. We hypothesized that reduced ectodomain release is a result of TNF/TNFR2 complex internalization. Indeed, we could demonstrate that TNFR2 was internalized together with its ligand and cytoplasmic binding partners. Upon endocytosis the TNFR2 signaling complex colocalized with late endosome/lysosome marker Rab7 and entered the lysosomal degradation pathway. Furthermore, we identified a di-leucin motif in the cytoplasmic part of TNFR2 suggesting clathrin-dependent internalization of TNFR2. Internalization defective TNFR2 mutants are capable to signal, i.e. activate NFκB, demonstrating that the di-leucin motif dependent internalization is dispensable for this response. We therefore propose that receptor internalization primarily serves as a negative feed-back to limit TNF responses via TNFR2. [ABSTRACT FROM AUTHOR]
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- 2011
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7. Dual function of cysteine rich domain (CRD) 1 of TNF receptor type 1: Conformational stabilization of CRD2 and control of receptor responsiveness
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Branschädel, Marcus, Aird, Andrew, Zappe, Andrea, Tietz, Carsten, Krippner-Heidenreich, Anja, and Scheurich, Peter
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TUMOR necrosis factors , *CYTOKINES , *RECEPTOR-ligand complexes , *MOLECULAR dynamics , *AMINO acids , *GLYCOPROTEINS - Abstract
Abstract: The proinflammatory cytokine Tumor Necrosis Factor (TNF) exists as a homotrimer, capable of binding three receptor molecules. However, signal competent ligand/receptor complexes form large clusters, likely to be stabilized by additional molecular interactions. Both TNF receptors, TNFR1 and TNFR2, contain four cysteine rich domains (CRD) in their extracellular parts. Previous work showed that the membrane distal CRD1 carries a homophilic interaction domain. Here, we investigated the functional role of CRD1 and its two submodules, A1CRD1 and B2CRD1, in a TNFR1-Fas chimera model system. Removal of CRD1 abolishes TNF binding. In line with these data, molecular dynamics simulations suggest that B2CRD1 of TNFR1 serves as a scaffold to stabilize CRD2 in a conformation necessary for high affinity ligand binding. Deletion of only the N-terminal half of CRD1 (ΔA1CRD1) of TNFR1 marginally affects ligand binding but abrogates responsiveness towards soluble TNF and reduces effectiveness as a dominant negative inhibitor of wild type TNFR1. A TNFR1-derived molecule containing the CRD1 from TNFR2 also shows reduced responsiveness to soluble TNF. These data strongly suggest that CRD1 is not only crucially involved in multimerization of unligated receptors, but is also directly involved in formation of signal competent ligand/receptor clusters, thereby controlling receptor responsiveness. [Copyright &y& Elsevier]
- Published
- 2010
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