8 results on '"Khaliq, Shagufta"'
Search Results
2. A spectrum of novel NPHS1 and NPHS2 gene mutations in pediatric nephrotic syndrome patients from Pakistan
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Abid, Aiysha, Khaliq, Shagufta, Shahid, Saba, Lanewala, Ali, Mubarak, Mohammad, Hashmi, Seema, Kazi, Javed, Masood, Tahir, Hafeez, Farkhanda, Naqvi, Syed Ali Anwar, Rizvi, Syed Adeebul Hasan, and Mehdi, Syed Qasim
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GENETIC mutation , *GENES , *NEPHROTIC syndrome , *JUVENILE diseases , *DISEASE prevalence - Abstract
Abstract: Background: Mutations in the NPHS1 and NPHS2 genes are among the main causes of early-onset and familial steroid resistant nephrotic syndrome respectively. This study was carried out to assess the frequencies of mutations in these two genes in a cohort of Pakistani pediatric NS patients. Methods: Mutation analysis was carried out by direct sequencing of the NPHS1 and NPHS2 genes in 145 nephrotic syndrome (NS) patients. This cohort included 36 samples of congenital or infantile onset NS cases and 39 samples of familial cases obtained from 30 families. Results: A total of 7 homozygous (6 novel) mutations were found in the NPHS1 gene and 4 homozygous mutations in the NPHS2 gene. All mutations in the NPHS1 gene were found in the early onset cases. Of these, one patient has a family history of NS. Homozygous p.R229Q mutation in the NPHS2 gene was found in two children with childhood-onset NS. Conclusions: Our results show a low prevalence of disease causing mutations in the NPHS1 (22% early onset, 5.5% overall) and NPHS2 (3.3% early onset and 3.4% overall) genes in the Pakistani NS children as compared to the European populations. In contrast to the high frequency of the NPHS2 gene mutations reported for familial SRNS in Europe, no mutation was found in the familial Pakistani cases. To our knowledge, this is the first comprehensive screening of the NPHS1 and NPHS2 gene mutations in sporadic and familial NS cases from South Asia. [Copyright &y& Elsevier]
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- 2012
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3. Association of specific single nucleotide variants (SNVs) in the promoter and 3'-Untranslated region of Vascular Endothelial growth factor (VEGF) gene with risk and higher tumour grade of head and neck cancers.
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Ajaz, Sadia, Muneer, Rabbia, Siddiqa, Aisha, Ali Memon, Muhammad, Firasat, Sadaf, Abid, Aiysha, and Khaliq, Shagufta
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SINGLE nucleotide polymorphisms , *VASCULAR endothelial growth factors , *HEAD & neck cancer , *TUMOR suppressor genes , *PROMOTERS (Genetics) , *SOUTH Asians , *RNA , *CASE-control method , *GENETIC polymorphisms , *NUCLEOTIDES , *GENES , *GENOTYPES , *DISEASE susceptibility , *TUMOR grading - Abstract
Background: Head and Neck Cancers (HNCs)comprise one of the most frequent cancers in South-Asian region. Vascular Endothelial Growth Factor (VEGF) has a potent role in tumorigenesis and metastasis. Certain common single nucleotide variants (SNVs) in the highly polymorphic VEGF gene are correlated with variations in VEGF functions. The data for these SNVs in HNCs is scarce for South Asian populations. The present study addresses this shortfall. It investigates the association of two VEGF SNVs, -2578C/A (rs699947) in the promoter region and + 936C/T (rs3025039) in 3'-UTR, with the risk of HNCs and tumour characteristics.Methods: The study comprised 323 participants with 121 HNC patients and 202 controls. Germline DNA was isolated from peripheral blood samples. PCR-RFLP methods were optimized and validated by Sanger sequencing. After Hardy-Weinberg evaluation, the independent associations were analyzed under the assumptions of different genetic models. The χ2 test of independence or Fisher's Exact test (significant p-values at < 0.05) were performed and ORs (odds ratios) with 95% confidence interval were tabulated.Results: VEGF -2578 A-allele, CA + AA, and AA genotypes had significant protective association against HNCs. The respective ORs were: 0.651 (0.469-0.904), 0.613 (0.381 - 0.985), and 0.393 (0.193-0.804). VEGF + 936 T-allele, CT, and CT + TT genotypes had significantly increased susceptibility for HNCs. The respective ORs were 1.882 (1.001 - 3.536), 2.060 (1.035 - 4.102), and 2.023 (1.032 - 3.966). Additionally, VEGF + 936 CT and CT + TT genotypes showed significant associations with higher tumour grade (p-values < 0.029, and < 0.037, respectively).Conclusion: The present study is the foremost report of independent and unique associations of the investigated VEGF SNVs with HNCs. [ABSTRACT FROM AUTHOR]- Published
- 2021
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4. The effect of chemokine receptor gene polymorphisms (CCR2V64I, CCR5-59029G>A and CCR5Δ32) on renal allograft survival in Pakistani transplant patients
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Firasat, Sadaf, Raza, Ali, Abid, Aiysha, Aziz, Tahir, Mubarak, Mohammad, Naqvi, Syed Ali Anwar, Rizvi, Syed Adeebul Hasan, Mehdi, Syed Qasim, and Khaliq, Shagufta
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CHEMOKINE receptors , *GENETIC polymorphisms , *HOMOGRAFTS , *TRANSPLANTATION of organs, tissues, etc. , *GRAFT rejection , *ORGAN donors - Abstract
Abstract: Background: Gene polymorphisms of the chemokine receptors CCR2 and CCR5 (CCR2V64I, CCR5-59029G>A and CCR5Δ32) have been shown to be associated with renal allograft rejection. The aim of this study was to investigate the association of these polymorphisms with allograft rejection among Pakistani transplant patients. Method: A total of 606 renal transplant patients and an equal number of their donors were included in this study. DNA samples were used to amplify polymorphic regions of CCR2V64I, CCR5-59029G>A and CCR5Δ32 by polymerase chain reaction using sequence specific primers. The amplified products of CCRV64I and CCR5-59029G>A were digested with restriction enzymes (BsaB1 and Bsp12861) respectively. The CCR5Δ32 genotypes were determined by sizing the PCR amplicons. The association of these polymorphisms with the biopsy proven rejection and other clinical parameters was evaluated using the statistical software SPSS v.17. Results: In this study, the G/G genotype of CCR2V64I was associated with a high frequency of allograft rejection (p=0.009; OR=2.14; 95% CI=1.2–3.7). Rejection episode(s) in the GA+AA genotypes were found to be significantly lower as compared to the GG genotype (p=0.009; OR=0.4; 95% CI=0.2–0.8). The Kaplan–Meier curve also indicated a reduced overall allograft survival for patients with the G/G genotype of CCR2V64I (59.2±1.4weeks, log p=0.008). There was a significant association with rejection by female donors possessing the CCR2 GG genotype (p=0.02; OR=2.6; CI=1.1–6.3) and male donors with the CCR5-59029 GG genotype (p=0.004; OR=1.7; CI=1.03–3.01). Conclusion: This study shows an association of the CCR2V64I (G/G) genotype with renal allograft rejection. However, no such association was found for the CCR5 gene polymorphisms. Therapeutic interventions such as blocking the CCR2 receptor (especially G polymorphism) may yield better survival of renal allograft in this patient group. Further, chemokine receptors may be added to the spectrum of the immunogenetic factors that are known to be associated with renal allograft rejection. [Copyright &y& Elsevier]
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- 2012
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5. Association of the ACE-II genotype with the risk of nephrotic syndrome in Pakistani children
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Shahid, Saba, Abid, Aiysha, Mehdi, Qasim Syed, Firasat, Sadaf, Lanewala, Ali, Naqvi, Ali Anwar Syed, Rizvi, Adib-ul-Hasan Syed, and Khaliq, Shagufta
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NEPHROTIC syndrome , *PROTEINURIA , *ANGIOTENSIN converting enzyme , *POLYMERASE chain reaction , *GENETIC polymorphisms , *DISEASE risk factors - Abstract
Abstract: Nephrotic syndrome is a common pediatric glomerular disease associated with heavy proteinuria. Since, the angiotensin converting enzyme (ACE) gene insertion/deletion (I/D) polymorphism is a putative genetic risk factor for NS, in this study, ACE (I/D) polymorphism was analyzed in 268 NS and 223 control samples by a PCR-based method. The genotypic and allelic frequencies were determined and the association between ACE I/D polymorphism and NS was evaluated. The frequency distribution of the II, ID and DD genotypes was 82 (30.6%), 128 (47.8%) and 58 (21.6%) in the NS patients and 9 (4.0%), 171 (76.7%) and 43 (19.3%) in the control samples respectively. In the Pakistani pediatric NS population, the II genotypic and allelic frequencies were found to be significantly associated with the disease (OR=6.755; C.I=3–14.9). No significant association was found between this polymorphism and the response to standard steroid therapy. Thus, in contrast to reports from other parts of the world, the II genotype was found to be significantly associated with NS in the Indian and Malay populations and in the Pakistani population described here. To our knowledge, this is the first report from Pakistan describing the association of the ACE I/D polymorphism with pediatric NS. On the basis of these results, it is suggested that analysis of the ACE (I/D) polymorphism should be performed for the early diagnosis in the high risk NS patients in South Asia. [Copyright &y& Elsevier]
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- 2012
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6. DC-SIGN Interacts with Mycobacterium leprae but Sequence Variation in This Lectin Is Not Associated with Leprosy in the Pakistani Population
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Barreiro, Luis B., Quach, Hélène, Krahenbuhl, James, Khaliq, Shagufta, Mohyuddin, Aisha, Mehdi, S. Qasim, Gicquel, Brigitte, Neyrolles, Olivier, and Quintana-Murci, Lluı́s
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LECTINS , *PATHOGENIC microorganisms , *MYCOBACTERIUM leprae , *HUMAN genetic variation , *MYCOBACTERIUM - Abstract
Abstract: The C-type lectin DC-SIGN is involved in early interactions between human innate immune cells and a variety of pathogens. Here we sought to evaluate whether DC-SIGN interacts with the leprosy bacillus, Mycobacterium leprae, and whether DC-SIGN genetic variation influences the susceptibility and/or pathogenesis of the disease. A case–control study conducted in a cohort of 272 individuals revealed no association between DC-SIGN variation and leprosy. However, our results clearly show that DC-SIGN recognizes M. leprae, indicating that mycobacteria recognition by this lectin is not as narrowly restricted to the Mycobacterium tuberculosis complex as previously thought. Altogether, our results provide further elucidation of M. leprae interactions with the host innate immune cells and emphasize the importance of DC-SIGN in the early interactions between the human host and the infectious agents. [Copyright &y& Elsevier]
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- 2006
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7. Analysis of the glutathione S-transferase genes polymorphisms in the risk and prognosis of renal cell carcinomas. Case-control and meta-analysis.
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Abid, Aiysha, Ajaz, Sadia, Khan, Abdul Rafay, Zehra, Fatima, Hasan, Asad Shahzad, Sultan, Gauhar, Mohsin, Rehan, Hashmi, Altaf, Niamatullah, Najeeb, Rizvi, Syed Adib-ul-Hasan, Mehdi, Syed Qasim, and Khaliq, Shagufta
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GLUTATHIONE , *GENETIC polymorphisms , *DELETION mutation , *RENAL cell carcinoma , *CANCER risk factors , *PROGNOSIS , *COMPARATIVE studies , *DISEASE susceptibility , *KIDNEY tumors , *RESEARCH methodology , *MEDICAL cooperation , *META-analysis , *RESEARCH , *TRANSFERASES , *EVALUATION research , *CASE-control method - Abstract
Background: The Glutathione S-transferases (GSTs) genes deletion polymorphisms have been associated with the progression of several cancers. The association studies between the 2 GSTs (GSTM1 and GSTT1) null polymorphisms with the susceptibility to renal cell carcinoma (RCC) have been inconclusive. Therefore, with the inclusion of our own data, we performed a comprehensive meta-analysis to assess the association between these 2 polymorphisms and the risk of RCC.Methods: A systematic literature search was carried out for studies published in the PubMed, EMBASE, Cochrane library, and Google Scholar from 1997 to December 2014. Results were stated as pooled odds ratios (ORs) for nonparametric data after heterogeneity analysis with 95% CI using fixed effect or random effect model.Results: We systematically selected 13 relevant studies after thorough searches from the databases. Data showed no association between the GSTM1 and the GSTT1 null genotypes and the risk of RCC (OR = 1.01; CI: 0.92-1.11; P = 0.89 for GSTM1 and OR = 1.14; CI: 0.91-1.42; P = 0.25 for GSTT1). No association was found when the data were stratified according to the geographical/ethnic basis, source of control, and the risk factor evaluation. Subgroup analysis of occupational exposure to pesticides showed an inverse association of the active genotypes of both GSTM1 and GSTT1 polymorphisms with the exposed group of RCC (P<0.00001 and P<0.00001, respectively). The combined null genotype of the GSTM1/GSTT1 significantly increased the susceptibility to RCC by 1.4-fold (P = 0.001). This association remained significant for the Asian populations in subgroup analysis (OR = 1.8; CI: 1.30-2.49; P = 0.0004).Conclusion: In conclusion, this meta-analysis suggests that the 2 GSTs deletion polymorphisms independently have no association with the risk of RCC. However, combination of both deletions increases the risk of developing the RCC. [ABSTRACT FROM AUTHOR]- Published
- 2016
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8. Erratum to “Association of the ACE-II genotype with the risk of nephrotic syndrome in Pakistani children” [Gene 493 (2012) 165–168]
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Shahid, Saba, Abid, Aiysha, Mehdi, Syed Qasim, Firasat, Sadaf, Lanewala, Ali, Naqvi, Syed Ali Anwar, Rizvi, Syed Adib-ul-Hasan, and Khaliq, Shagufta
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- 2012
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