1. Mi-2β Associates with BRG1 and RET Finger Protein at the Distinct Regions with Transcriptional Activating and Repressing Abilities.
- Author
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Shimono, Yohei, Murakami, Hideki, Kawai, Kumi, Wade, Paul A., Shimokata, Kaoru, and Takahashi, Masahide
- Subjects
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GENE expression , *GENETIC transcription regulation , *HISTONES , *PROTEINS , *GENETIC repressors , *CHROMATIN - Abstract
Mi-2β is the main component of the nucleosome remodeling and deacetylase complex and plays an important role in epigenetic transcriptional repression. Here we show that the amino-terminal and carboxyl-terminal regions of Mi-2β have distinct transcriptional activities and bind to BRG1, a component of the SWI/SNF complex, and the RET finger protein (RFP), respectively. Analysis by luciferase reporter assay revealed that the amino-terminal region of Mi-2β has a strong transactivating ability, whereas its carboxyl-terminal region has transcriptional repressive activity. Co-localization and association of Mi-2, RFP, and histone deacetylase 1 suggested that these proteins cooperate in transcriptional repression. Furthermore, the functional importance of the association of Mi-2β and RFP was confirmed by using Rfp[sup -/-] fibroblasts. On the other hand, we demonstrated that Mi-2 and BRG1 were associated with each other and that the bromodomain region of BRG1 strongly suppressed transactivation by the amino-terminal region of Mi-2β. The findings that Mi-2β interacts with both transactivating and repressing proteins and directly associates with another chromatin remodeling protein, BRG1, provide new insight into the formation of multiprotein supercomplex involved in transcriptional regulation. [ABSTRACT FROM AUTHOR]
- Published
- 2003
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