1. A novel P3H1 mutation is associated with osteogenesis imperfecta type VIII and dental anomalies.
- Author
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Kantaputra, Piranit Nik, Dejkhamron, Prapai, Intachai, Worrachet, Ngamphiw, Chumpol, Ketudat Cairns, James R., Kawasaki, Katsushige, Ohazama, Atsushi, Olsen, Bjorn, Tongsima, Sissades, and Angkurawaranon, Salita
- Abstract
Objective: Our objective was to investigate the molecular etiology of osteogenesis imperfecta type VIII and dental anomalies in 4 siblings of a Karen tribe family.Materials and Methods: Four patients and their unaffected parents were studied by clinical and radiographic examination. In situ hybridization of P3h1 during early murine tooth development, whole-exome sequencing, and Sanger direct sequencing were performed.Results: A novel homozygous missense P3H1 mutation (NM_001243246.1; c.2141A>G; NP_001230175.1; p.Lys714Arg) was identified in all patients. Their unaffected parents were heterozygous for the mutation. The mutation is hypothesized to belong to isoform c of P3H1. Mutations in P3H1 are associated with autosomal recessive osteogenesis imperfecta type VIII. Hypodontia, a mesiodens, and single-rooted permanent second molars found in our patients have never been reported in patients with P3H1 mutations. Single-rooted second permanent molars or failure to form multiple roots implies effects of the P3H1 mutation on root development.Conclusions: We report a novel P3H1 mutation as the underlying cause of osteogenesis imperfecta type VIII with dental anomalies. Our study suggests that isoform c of P3H1 is also a functional isoform of P3H1. We report, for the first time, to our knowledge, the association of P3H1 mutation and osteogenesis imperfecta type VIII with dental anomalies. [ABSTRACT FROM AUTHOR]- Published
- 2021
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