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Few studies reported the application of miRNA in bone regeneration. In this study, the expression of miR133a and miR133b in murine BMSCs was inhibited via antagomiR-133a/b and the osteogenic differentiation in murine BMSCs was evaluated. The RT-PCR, flow cytometry, cell counting kit-8, and annexin V-FITC/PI double staining assays were performed. Double knockdown miR133a and miR133b can promote BMSC osteogenic differentiation. At optimum N/P ration (15:1), the loading efficiency can reach over 90%. CTH-antagomiR-133a/b showed no cytotoxicity to BMSCs and diminished miR133a and miR133b expression in BMSCs. Furthermore, chitosan-based sustained delivery system can facilitate continuous dosing of antagomiR-133a/b, which enhanced calcium deposition and osteogenic specific gene expression in vitro. The new bone formation was enhanced after the sustained delivery system containing CTH-antagomiR-133a/b nanoparticles was used in mouse calvarial bone defect model. Our results demonstrate that CTH nanoparticles could facilitate continuous dosing of antagomiR133a/b, which can promote osteogenic differentiation. The new bone formation was enhanced after the sustained delivery system containing CTH-antagomiR-133a/b nanoparticles was used in mouse calvarial bone defect model. Above results demonstrated that continuous dosing of antagomiR133a/b can promote osteogenic differentiation and CTH nanoparticles could be considered as a potential candidate as an efficient non-viral miRNA carrier for the miRNA-based therapy. Unlabelled Image [ABSTRACT FROM AUTHOR]

Abstract: Ordered mesoporous carbon (OMC)–SnO2 nanocomposite was prepared by a melt injection method using SnCl2·2H2O as the tin source. The results of X-ray diffraction (XRD) indicated that tin dioxide in the nanocomposite was crystalline. The BJH adsorption average pore size was greatly reduced from 5.1nm for OMC to 2.9nm for the mesoporous OMC–SnO2 composite which is due to the partial occupation of the pores by the tin species incorporated into the mesoporous carbon. The OMC–SnO2 electrode exhibits excellent cycle stability: the retentive capacity (472.1mAh/g) up to 30 cycles is 62.2% of the initial capacity (758.2mAh/g) which is much better than that for commercial SnO2. These results suggest that OMC–SnO2 nanocomposite would be a promising anode material for lithium ion battery. [Copyright &y& Elsevier]