1. Aging dependent effect of nuclear tau
- Author
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Concetta Federico, Laura Gil, Isidre Ferrer, Ana Belén Rebolledo, Fernando Pinedo, Juan José Montoya, Francesca Bruno, Salvatore Saccone, Isabel M. Olazabal, and Universitat de Barcelona
- Subjects
0301 basic medicine ,Pathology ,Aging ,Nucleolus ,Physiology ,Fluorescent Antibody Technique ,Hippocampus ,Severity of Illness Index ,Heterochromatin ,Breast ,Intestinal Mucosa ,Phosphorylation ,AT100 ,Cellular Senescence ,Cancer ,Neurons ,biology ,General Neuroscience ,Pyramidal Cells ,Middle Aged ,Metabolisme ,Chromatin ,Cell biology ,medicine.anatomical_structure ,Nuclis cel·lulars ,Hippocampus (Brain) ,Cell aging ,Adult ,medicine.medical_specialty ,Hipocamp (Cervell) ,Tau protein ,Fisiologia ,Breast Neoplasms ,tau Proteins ,Cell nuclei ,Breast acinar cells ,03 medical and health sciences ,Young Adult ,Aging marker ,Alzheimer disease ,Colorectal mucosa ,Confocal microscopy ,Epithelial cells ,Nuclear tau ,Neuroscience (all) ,Molecular Biology ,Neurology (clinical) ,Developmental Biology ,Alzheimer Disease ,Envelliment ,medicine ,Humans ,Cell Nucleus ,Carcinoma, Acinar Cell ,Dentate gyrus ,Epithelial Cells ,030104 developmental biology ,Metabolism ,nervous system ,Cytoplasm ,Cancer cell ,biology.protein ,Nucleus - Abstract
Tau protein is characterized by a complex pattern of phosphorylation and is localized in the cytoplasm and nucleus in both neuronal and non-neuronal cells. Human AT100 nuclear tau, endowed by phosphorylation in Thr212/Ser214, was recently shown to decline in cornus ammonis 1 (CA1) and dentate gyrus (DG) in Alzheimer's disease (AD), but a defined function for this nuclear tau remains unclear. Here we show that AT100 progressively increases in the nuclei of neuronal and non-neuronal cells during aging, and decreases in the more severe AD stages, as recently shown, and in cancer cells (colorectal adenocarcinoma and breast cancer). AT100, in addition to a co-localization with the DAPI-positive heterochromatin, was detected in the nucleolus of pyramidal cells from the CA1 region, shown to be at its highest level in the more senescent cells and in the first stage of AD (ADI), and disappearing in the more severe AD cases (ADIV). Taking into account the nuclear distribution of AT100 during cell aging and its relation to the chromatin changes observed in degenerated neurons, as well as in cancerous cells, which are both cellular pathologies associated with age, we can consider the Thr212/Ser214 phosphorylated nuclear tau as a molecular marker of cell aging.
- Published
- 2017