11 results on '"Hung, C-T."'
Search Results
2. Shearing of synovial fluid activates latent TGF-β.
- Author
-
Albro MB, Cigan AD, Nims RJ, Yeroushalmi KJ, Oungoulian SR, Hung CT, Ateshian GA, Albro, M B, Cigan, A D, Nims, R J, Yeroushalmi, K J, Oungoulian, S R, Hung, C T, and Ateshian, G A
- Abstract
Objective: TGF-β is synthesized in an inactive latent complex that is unable to bind to membrane receptors, thus unable to induce a cellular biological response until it has been activated. In addition to activation by chemical mediators, recent studies have demonstrated that mechanical forces may activate latent TGF-βvia integrin-mediated cellular contractions, or mechanical shearing of blood serum. Since TGF-β is present in synovial fluid in latent form, and since normal diarthrodial joint function produces fluid shear, this study tested the hypothesis that the native latent TGF-β1 of synovial fluid can be activated by shearing.Design: Synovial fluid from 26 bovine joints and three adult human joints was sheared at mean shear rates up to 4000 s(-1) for up to 15 h.Results: Unsheared synovial fluid was found to contain high levels of latent TGF-β1 (4.35 ± 2.02 ng/mL bovine, 1.84 ± 0.89 ng/mL human; mean ± radius of 95% confidence interval) and low amounts (<0.05 ng/mL) of the active peptide. Synovial fluid concentrations of active TGF-β1 increased monotonically with shear rate and shearing duration, reaching levels of 2.64 ± 1.22 ng/mL for bovine and 0.60 ± 0.39 ng/mL for human synovial fluid. Following termination of shearing, there was no statistical change in these active levels over the next 8 h for either species, demonstrating long-term stability of the activated peptide. The unsheared control group continued to exhibit negligible levels of active TGF-β1 at all times.Conclusions: Results confirmed the hypothesis of this study and suggest that shearing of synovial fluid might contribute an additional biosynthetic effect of mechanical loading of diarthrodial joints. [ABSTRACT FROM AUTHOR]- Published
- 2012
- Full Text
- View/download PDF
3. Development of a parallel semi-implicit two-dimensional plasma fluid modeling code using finite-volume method
- Author
-
Lin, K.-M., Hung, C.-T., Hwang, F.-N., Smith, M.R., Yang, Y.-W., and Wu, J.-S.
- Subjects
- *
PLASMA gases , *MATHEMATICAL models , *ELECTRIC discharges , *FINITE volume method , *CODING theory , *PERFORMANCE evaluation , *CHEMICAL reactions - Abstract
Abstract: In this paper, the development of a two-dimensional plasma fluid modeling code using the cell-centered finite-volume method and its parallel implementation on distributed memory machines is reported. Simulated discharge currents agree very well with the measured data in a planar dielectric barrier discharge (DBD). Parallel performance of simulating helium DBD solved by the different degrees of overlapping of additive Schwarz method (ASM) preconditioned generalized minimal residual method (GMRES) for different modeling equations is investigated for a small and a large test problem, respectively, employing up to 128 processors. For the large test problem, almost linear speedup can be obtained by using up to 128 processors. Finally, a large-scale realistic two-dimensional DBD problem is employed to demonstrate the capability of the developed fluid modeling code for simulating the low-temperature plasma with complex chemical reactions. [Copyright &y& Elsevier]
- Published
- 2012
- Full Text
- View/download PDF
4. Effect of plasma chemistry on the simulation of helium atmospheric-pressure plasmas
- Author
-
Chiu, Y.-M., Hung, C.-T., Hwang, F.-N., Chiang, M.-H., Wu, J.-S., and Chen, S.-H.
- Subjects
- *
PLASMA chemistry , *SIMULATION methods & models , *HELIUM plasmas , *ATMOSPHERIC pressure , *ELECTRIC discharges , *NUMERICAL analysis , *SELF-consistent field theory , *ABSORPTION - Abstract
Abstract: The effect on the selection of different plasma chemistries for simulating a typical dielectric barrier discharge (DBD) driven by quasi-pulsed power source (20 kHz) is investigated. The numerical simulation was performed by using the one-dimensional self-consistent fluid modeling solver. Our simulation result indicates that the computed temporal current density can be significantly improved by using a complex version of plasma chemistry module rather than the simple one and demonstrates an excellent agreement with the experimental data. The result suggests the metastable, excited and ionic helium related reaction channels, which are important in simulating a DBD, should be taken into account. Furthermore, it also reveals that the power absorption of ions is considerably higher than that of the electron. [Copyright &y& Elsevier]
- Published
- 2011
- Full Text
- View/download PDF
5. One-dimensional simulation of nitrogen dielectric barrier discharge driven by a quasi-pulsed power source and its comparison with experiments
- Author
-
Cheng, K.-W., Hung, C.-T., Chiang, M.-H., Hwang, F.-N., and Wu, J.-S.
- Subjects
- *
SIMULATION methods & models , *NITROGEN , *DIELECTRICS , *ELECTRIC discharges , *SELF-consistent field theory , *FLUID dynamics , *PHASE transitions - Abstract
Abstract: Parallel-plate nitrogen dielectric barrier discharge (DBD) driven by a quasi-pulsed power source (60 kHz) is simulated using a fully-implicit 1D self-consistent fluid modeling code. Simulated discharged currents of gap distances (0.5, 0.7 and 1.0 mm) agree very well with measured data; while simulated discharged currents of wider gap distance (1.2 mm) fail to reproduce the measured data. It is found that the discharge mode is homogeneous Townsend-like for the former case; while it is filamentary-like for the latter case based on the experimental observation. These findings demonstrate that previous numerical studies showing mode transition by changing the gap distance may require further investigation. [Copyright &y& Elsevier]
- Published
- 2011
- Full Text
- View/download PDF
6. A new paradigm for solving plasma fluid modeling equations
- Author
-
Hung, C.-T., Hu, M.-H., Wu, J.-S., and Hwang, F.-N.
- Published
- 2007
- Full Text
- View/download PDF
7. Dexmedetomidine infusion as a supplement to isoflurane anaesthesia for vitreoretinal surgery.
- Author
-
Lee, Y Y S, Wong, S M, and Hung, C T
- Abstract
Background: We explored the sympatholytic property of dexmedetomidine, especially its role in intraocular pressure (IOP) reduction, haemodynamic stability, and attenuation of extubation response.Method: In this double-blind, randomized, controlled trial approved by the Hospital Ethics Committee, 60 patients undergoing elective vitreoretinal surgery were allocated to two groups, receiving either placebo or dexmedetomidine. A loading dose of dexmedetomidine 2.5 microg kg(-1) h(-1) (or placebo in same volume) was infused for 10 min immediately before induction of anaesthesia with propofol, followed by a maintenance dexmedetomidine or placebo infusion at 0.4 microg kg(-1) h(-1) till 30 min before the end of the operation. Anaesthesia was maintained with isoflurane, oxygen, and air mixture. IOP was measured before the loading dose and 1 min after tracheal intubation. The mean arterial pressure (MAP) and heart rate (HR) during loading, induction, maintenance, extubation, and recovery period were measured. The degree of strain on extubation was graded from 0 to 5.Results: The use of vasopressor/labetalol/atropine and the reduction in IOP were comparable between the two groups. There was a significant variation in MAP and HR over time within group, but not between groups. The median degree of strain was significantly lower (P = 0.049), and the time to reach Aldrete score of 10 shorter (P = 0.031) in the dexmedetomidine group.Conclusion: Dexmedetomidine can be used without undue haemodynamic fluctuation and can decrease the excitatory response during extubation. The reduction in IOP with dexmedetomidine was comparable with placebo. [ABSTRACT FROM AUTHOR]- Published
- 2007
- Full Text
- View/download PDF
8. A parallel hybrid numerical algorithm for simulating gas flow and gas discharge of an atmospheric-pressure plasma jet
- Author
-
Lin, K.-M., Hu, M.-H., Hung, C.-T., Wu, J.-S., Hwang, F.-N., Chen, Y.-S., and Cheng, G.
- Subjects
- *
ALGORITHMS , *COMPUTER simulation of gas flow , *GLOW discharges , *ATMOSPHERIC pressure , *PLASMA jets , *MATHEMATICAL models , *PARALLEL computers - Abstract
Abstract: Development of a hybrid numerical algorithm which couples weakly with the gas flow model (GFM) and the plasma fluid model (PFM) for simulating an atmospheric-pressure plasma jet (APPJ) and its acceleration by two approaches is presented. The weak coupling between gas flow and discharge is introduced by transferring between the results obtained from the steady-state solution of the GFM and cycle-averaged solution of the PFM respectively. Approaches of reducing the overall runtime include parallel computing of the GFM and the PFM solvers, and employing a temporal multi-scale method (TMSM) for PFM. Parallel computing of both solvers is realized using the domain decomposition method with the message passing interface (MPI) on distributed-memory machines. The TMSM considers only chemical reactions by ignoring the transport terms when integrating temporally the continuity equations of heavy species at each time step, and then the transport terms are restored only at an interval of time marching steps. The total reduction of runtime is 47% by applying the TMSM to the APPJ example presented in this study. Application of the proposed hybrid algorithm is demonstrated by simulating a parallel-plate helium APPJ impinging onto a substrate, which the cycle-averaged properties of the 200th cycle are presented. The distribution patterns of species densities are strongly correlated by the background gas flow pattern, which shows that consideration of gas flow in APPJ simulations is critical. [Copyright &y& Elsevier]
- Published
- 2012
- Full Text
- View/download PDF
9. Development of a parallel Poisson's equation solver with adaptive mesh refinement and its application in field emission prediction
- Author
-
Hsu, K.-H., Chen, P.-Y., Hung, C.-T., Chen, L.-H., and Wu, J.-S.
- Subjects
- *
POISSON'S equation , *GALERKIN methods , *FINITE element method , *ELLIPTIC differential equations - Abstract
Abstract: A parallel electrostatic Poisson''s equation solver coupled with parallel adaptive mesh refinement (PAMR) is developed in this paper. The three-dimensional Poisson''s equation is discretized using the Galerkin finite element method using a tetrahedral mesh. The resulting matrix equation is then solved through the parallel conjugate gradient method using the non-overlapping subdomain-by-subdomain scheme. A PAMR module is coupled with this parallel Poisson''s equation solver to adaptively refine the mesh where the variation of potentials is large. The parallel performance of the parallel Poisson''s equation is studied by simulating the potential distribution of a CNT-based triode-type field emitter. Results with ∼100 000 nodes show that a parallel efficiency of 84.2% is achieved in 32 processors of a PC-cluster system. The field emission properties of a single CNT triode- and tetrode-type field emitter in a periodic cell are computed to demonstrate their potential application in field emission prediction. [Copyright &y& Elsevier]
- Published
- 2006
- Full Text
- View/download PDF
10. Development of a parallelized 3D electrostatic PIC-FEM code and its applications
- Author
-
Wu, J.-S., Hsu, K.-H., Li, F.-L., Hung, C.-T., and Jou, S.-Y.
- Subjects
- *
ELECTROSTATICS , *COMPUTER simulation , *SIMULATION methods & models , *MAGNETRONS , *ARGON plasmas , *FIELD emission - Abstract
A parallelized three-dimensional self-consistent electrostatic particle-in-cell (PIC) code using unstructured tetrahedral mesh is proposed. Parallel implementation of the current unstructured PIC-FEM code is realized on distributed-memory PC-cluster system utilizing dynamic domain decomposition. Completed code is verified by simulating a quasi-1D RF argon gas discharge with results comparable to previous experimental observations and simulations. Parallel performance with dynamic domain decomposition of the PIC code is tested using a 3D RF argon gas discharge on a PC-cluster system. Results show that parallel efficiency can achieve 83% at 32 processors with dynamic domain decomposition. Some possible improvement of the code performance is demonstrated. Completed code is then applied to predict field emission without and with space-charge effect, and to simulate the RF magnetron argon plasma to demonstrate its capability in handling practical problems. [Copyright &y& Elsevier]
- Published
- 2007
- Full Text
- View/download PDF
11. High-Mobility Group Box 1 Protein Activates Hepatic Stellate Cells In Vitro
- Author
-
Kao, Y.-H., Jawan, B., Goto, S., Hung, C.-T., Lin, Y.-C., Nakano, T., Hsu, L.-W., Lai, C.-Y., Tai, M.-H., and Chen, C.-L.
- Subjects
- *
BLOOD plasma , *METALLOPROTEINASES , *LIVER transplantation , *SMOOTH muscle - Abstract
Abstract: Objectives: Our previous study noticed remarkably elevated titers of anti–high-mobility group box 1 (HMGB1) antibodies in sera during the tolerance induction phase of a rat tolerogenic orthotopic liver transplantation (OLT) as well as in sera of clinically drug-free patients. We hypothesized that the release of nonhistone nuclear protein HMGB1 during rejection may play a pathogenic role in deteriorating post-OLT graft functions, such as inducing liver fibrosis. This study sought to investigate whether HMGB1 can directly activate hepatic stellate cells (HSCs) and drive them toward fibrogenesis. Methods: The cultured HSCs were treated with recombinant HMGB1. RT-PCR and Western blotting analysis were used to measure α-smooth muscle actin (α-SMA) expression. Conditioned media were collected for gelatin zymography to monitor the activities of collagen-degrading matrix metalloproteinases (MMPs). Results: HMGB1 at concentrations >1 ng/mL significantly stimulated HSC growth as revealed by proliferation and BrdU assays. α-SMA gene and protein expression were significantly up-regulated by HMGB1, whereas the MMP-2, but not MMP-9, activity was suppressed by HMGB1 treatment. Conclusion: Our data suggested that HMGB1 protein, once released during the rejection phase of OLT, activated HSCs and exhibited profibrogenic effects on liver grafts either by increasing the HSC population and extracellular matrix content in liver grafts, or by transforming HSCs into myofibroblasts. Neutralization with anti-HMGB1 antibody was suggested to be a therapeutic modality applicable to prevent fibrogenesis in post-OLT liver grafts. [Copyright &y& Elsevier]
- Published
- 2008
- Full Text
- View/download PDF
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.