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Start Over Author "Hu, Di" Publisher elsevier b.v.
163 results on '"Hu, Di"'

46. Identification of Biomarkers in Intracranial Aneurysm and Their Immune Infiltration Characteristics.

Author

Huang, Cheng, Hu, Di, and Li, Keshen

Subjects
*INTRACRANIAL aneurysms, *BIOMARKERS, *CEREBROVASCULAR disease, *TEMPORAL arteries, *DRUG target, *INTRACRANIAL aneurysm ruptures
Abstract

Intracranial aneurysm (IA), known as the intracranial "unscheduled bomb," is one of the most dangerous cerebrovascular diseases, with unclear pathogenesis. This study aimed to show the mechanisms and identify the new biological targets by applying bioinformatics analysis. Expression profiling for control superficial temporal artery and IA walls in GSE26969 and GSE75436 datasets were downloaded. By executing the LIMMA package in R software, the differentially expressed genes (DEGs) were filtered, and the functional enrichments were consequently performed. Further cross-linking with the 2483 immune-related genes (IRGs) from the ImmPort database, the differentially expressed IRGs were identified. Based on them, the least absolute shrinkage and selection operator logistic regression and support vector machine–recursive feature elimination algorithms were used to screen the biomarkers, which were validated in the GSE54083 datasets. The CIBERSORT algorithm was applied to evaluate the infiltration of immune cells in tissues. A total of 668 DEGs were obtained, and the functional enrichment suggested that they were closely related to the immune process. After intersecting them with the IRGs, 90 differentially expressed IRGs emerged, and ADIPOQ and ESM1 were identified as the biomarkers. Besides, we found that the infiltrated immune cells, such as the mast cells resting, might be associated with them. We explored the contributing factors involving IA, which may generate a better understanding of the complex interactions among them and inspire a promising strategy for clinical works. [ABSTRACT FROM AUTHOR]

Published
2022
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48. Green and sustainable extraction of phycocyanin from Spirulina platensis by temperature-sensitive polymer-based aqueous two-phase system and mechanism study.

Author

Hu, Di, Xu, Ruru, Jin, Yating, Sun, Shuqing, Ye, Jing, Wu, Jiajia, Dai, Zhiyuan, Shen, Jia-Wei, and Lu, Yanbin

Subjects
*THERMORESPONSIVE polymers, *MOLECULAR dynamics, *SPIRULINA platensis, *MOLECULAR docking, *RESPONSE surfaces (Statistics)
Abstract

[Display omitted] • A green and sustainable extraction method of PC from S. platensis was established. • Pluronic F68, a temperature-sensitive polymer-based ATPS was evaluated and optimized. • The polymer can be recovered and reused, indicating a recyclable extraction process. • Interaction of PC and Pluronic F68 was studied by spectral and simulation approaches. • Hydrophobic segment of Pluronic F68 played a key role in the binding process with PC. In this study, a sustainable and environmentally friendly method was developed for the enrichment and purification of phycocyanin from Spirulina platensis. This was achieved by utilizing a temperature-sensitive polymer, Pluronic F68, in an aqueous two-phase solvent system. The phase behavior of the temperature-sensitive polymer-based biphasic system was evaluated. The extraction conditions were optimized by both single-factor experiments and response surface methodology. Under the optimal conditions, the upper polymer-rich phase was recycled for sustainable phycocyanin extraction, resulting in a grade of 3.23 during the third extraction cycle. Pluronic F68 could be efficiently recovered and reused during the extraction process. The interaction mechanism between Pluronic F68 and phycocyanin was systematically studied using FT-IR and fluorescence analysis. This was further complemented by static and dynamic calculation of molecular motion through molecular docking and molecular dynamics simulation, indicating that hydrophobic segment of Pluronic F68 played a key role in the binding process with phycocyanin. [ABSTRACT FROM AUTHOR]

Published
2024
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