1. Arthropod promoters for genetic control of disease vectors.
- Author
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Wudarski, Jakub, Aliabadi, Simindokht, and Gulia-Nuss, Monika
- Subjects
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CRISPRS , *MOSQUITO control , *VECTOR control , *GENETIC disorders , *MALARIA , *GENE expression , *TRANSGENE expression , *LYME disease - Abstract
Genetic toolkit is now extended to disease vectors. For genetic control methods such as clustered regularly interspaced short palindromic repeats (CRISPR)/Cas-based editing and gene drives, promoters with robust and tissue-specific expression are needed. The availability of genomes and machine-learning technologies provides an opportunity to identify promoters in non-model vectors. Vector-borne diseases (VBDs) impose devastating effects on human health and a heavy financial burden. Malaria, Lyme disease, and dengue fever are just a few examples of VBDs that cause severe illnesses. The current strategies to control VBDs consist mainly of environmental modification and chemical use, and to a small extent, genetic approaches. The genetic approaches, including transgenesis/genome modification and gene-drive technologies, provide the basis for developing new tools for VBD prevention by suppressing vector populations or reducing their capacity to transmit pathogens. The regulatory elements such as promoters are required for a robust sex-, tissue-, and stage-specific transgene expression. As discussed in this review, information on the regulatory elements is available for mosquito vectors but is scant for other vectors. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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