16 results on '"Gokhale, Rajesh S."'
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2. Bioscience in India: Times Are Changing
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Dhawan, Jyotsna, Gokhale, Rajesh S., and Verma, Inder M.
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Biological sciences - Abstract
To link to full-text access for this article, visit this link: http://dx.doi.org/10.1016/j.cell.2005.11.018 Byline: Jyotsna Dhawan (1), Rajesh S. Gokhale (2), Inder M. Verma (3) Abstract: India has a unique opportunity to become a leader in biological sciences research. In this commentary, we address what India needs to do to achieve this goal. Author Affiliation: (1) Center for Cellular and Molecular Biology Uppal Road, Hyderabad 500007, India (2) National Institute of Immunology, Aruna Asaf Ali Marg, New Delhi 110067, India (3) Laboratory of Genetics, The Salk Institute, La Jolla, CA 920373, USA
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- 2005
3. Fatty acyl-AMP ligases and polyketide synthases are unique enzymes of lipid biosynthetic machinery in Mycobacterium tuberculosis.
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Mohanty, Debasisa, Sankaranarayanan, Rajan, and Gokhale, Rajesh S.
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LIGASES ,FATTY acids ,POLYKETIDES ,LIPID metabolism ,BIOSYNTHESIS ,MYCOBACTERIUM tuberculosis ,PROTEIN structure ,HOMOLOGY (Biology) - Abstract
Summary: The cell envelope of Mycobacterium tuberculosis (Mtb) possesses a repertoire of unusual lipids that are believed to play an important role in pathogenesis. In this review, we specifically focus on computational, biochemical and structural studies in lipid biosynthesis that have established functional role of polyketide synthases (PKSs) and fatty acyl-AMP ligases (FAALs). Mechanistic and structural studies with FAALs suggest that this group of proteins may have evolved from omnipresent fatty acyl-CoA ligases (FACLs). FAALs activate fatty acids as acyl-adenylates and transfer them on to the PKSs which then produce unusual acyl chains that are the components of mycobacterial lipids. FAALs are a newly discovered family of enzymes; whereas involvement of PKSs in lipid metabolism was not known prior to their discovery in Mtb. Since Mtb genome contains multiple homologs of FAALs and PKSs and owing to the conserved reaction mechanism and overlapping substrate specificity; there is tempting opportunity to develop ‘systemic drugs’ against these enzymes as anti-tuberculosis agents. [Copyright &y& Elsevier]
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- 2011
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4. Chapter 12 Polyketide Versatility in the Biosynthesis of Complex Mycobacterial Cell Wall Lipids.
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Chopra, Tarun and Gokhale, Rajesh S.
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Abstract: Genome sequencing of Mycobacterium tuberculosis (Mtb) has revealed a large number of open reading frames homologous to polyketide synthases (PKSs). Since Mtb is not known to produce secondary metabolites, their presence in the Mtb genome was rather surprising. Research over the last decade has demonstrated that these PKSs are involved in the biosynthesis and assembly of complex lipids. The catalytic as well as mechanistic versatility of PKSs in producing acyl chains of Mtb lipidic metabolites are discussed here. We refer to the literature for most bench-level details, but include protocols for generally useful methods for analyzing the products of mycobacterial PKSs. [Copyright &y& Elsevier]
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- 2009
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5. Versatility of polyketide synthases in generating metabolic diversity
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Gokhale, Rajesh S, Sankaranarayanan, Rajan, and Mohanty, Debasisa
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POLYKETIDES , *KETENES , *BIOSYNTHESIS , *LIPIDS , *DICTYOSTELIUM discoideum , *DICTYOSTELIUM - Abstract
Polyketide synthases (PKSs) form a large family of multifunctional proteins involved in the biosynthesis of diverse classes of natural products. Architecturally at least three different types of PKSs have been discovered in the microbial world and recent years have revealed tremendous versatility of PKSs, both in terms of their structural and functional organization and in their ability to produce compounds other than typical secondary metabolites. Mycobacterium tuberculosis exploits polyketide biosynthetic enzymes to synthesize complex lipids, many of which are essential for its survival. The functional significance of the large repertoire of PKSs in Dictyostelium discoideum, perhaps in producing developmental regulating factors, is emerging. Recently determined structures of fatty acid synthases (FASs) and PKSs now provide an opportunity to delineate the mechanistic and structural basis of polyketide biosynthetic machinery. [Copyright &y& Elsevier]
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- 2007
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6. Tuberculosis research: Staying ahead of the game.
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Gokhale, Rajesh S.
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- 2011
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7. Network, nodes and nexus: systems approach to multitarget therapeutics.
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Murthy, Divya, Attri, Kuldeep Singh, and Gokhale, Rajesh S
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BIOLOGICAL networks , *NODES of Ranvier , *SYSTEMS biology , *SPATIO-temporal variation , *PHENOTYPES , *GENE targeting , *BIOLOGICAL adaptation - Abstract
Systems biology is revealing multiple layers of regulatory networks that manifest spatiotemporal variations. Since genes and environment also influence the emergent property of a cell, the biological output requires dynamic understanding of various molecular circuitries. The metabolic networks continually adapt and evolve to cope with the changing milieu of the system, which could also include infection by another organism. Such perturbations of the functional networks can result in disease phenotypes, for instance tuberculosis and cancer. In order to develop effective therapeutics, it is important to determine the disease progression profiles of complex disorders that can reveal dynamic aspects and to develop mutitarget systemic therapies that can help overcome pathway adaptations and redundancy. [Copyright &y& Elsevier]
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- 2013
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8. Identification and Characterization of a Type III Polyketide Synthase Involved in Quinolone Alkaloid Biosynthesis from Aegle marmelos Correa.
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Resmi, Mohankumar Saraladevi, Verma, Priyanka, Gokhale, Rajesh S., and Soniya, Eppurathu Vasudevan
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QUINOLONE antibacterial agents , *AMINO acids , *ORGANONITROGEN compounds , *HOMOLOGY (Biology) , *ALKALOIDS - Abstract
Quinolone alkaloids, found abundantly in the roots of bael (Aegle marmelos), possess various biological activities and have recently gained attention as potential lead molecules for novel drug designing. Here, we report the characterization of a novel Type III polyketide synthase, quinolone synthase (QNS), from A. marmelos that is involved in the biosynthesis of quinolone alkaloid. Using homology-based structural modeling, we identify two crucial amino acid residues (Ser-132 and Ala-133) at the putative QNS active site. Substitution of Ser-132 to Thr and Ala-133 to Ser apparently constricted the active site cavity resulting in production of naringenin chalcone from p-coumaroyl- CoA. Measurement of steady-state kinetic parameters demonstrates that the catalytic efficiency of QNS was severalfold higher for larger acyl-coenzyme A substrates as compared with smaller precursors. Our mutagenic studies suggest that this protein might have evolved from an evolutionarily related member of chalcone synthase superfamily by mere substitution of two active site residues. The identification and characterization of QNS offers a promising target for gene manipulation studies toward the production of novel alkaloid scaffolds. [ABSTRACT FROM AUTHOR]
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- 2013
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9. Synthesis, biological evaluation and docking studies of silicon incorporated diarylpyrroles as MmpL3 inhibitors: An effective strategy towards development of potent anti-tubercular agents.
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Vasudevan, N., Motiwala, Zenia, Ramesh, Remya, Wagh, Sachin B., Shingare, Rahul D., Katte, Revansiddha, Anand, Amitesh, Choudhary, Sushil, Kumar, Ajay, Gokhale, Rajesh S., Kulkarni, Kiran A., and Reddy, D. Srinivasa
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ANTITUBERCULAR agents , *MOLECULAR docking , *COLLECTIVE efficacy , *MYCOBACTERIUM tuberculosis , *SILICON - Abstract
Growing global demand for new molecules to treat tuberculosis has created an urgent need to develop novel strategies to combat the menace. BM212 related compounds were found to be potent anti-TB agents and they inhibit mycolic acid transporter, MmpL3, a known potent drug target from Mycobacterium tuberculosis. In order to enhance their inhibitory potency, several silicon analogues of diarylpyrroles related to BM212 were designed, synthesized, and evaluated for anti-tubercular activities. In Alamar blue assay, most of the silicon-incorporated compounds were found to be more potent than the parent compound (BM212), against Mycobacterium tuberculosis (MIC = 1.7 μM, H37Rv). Docking results from the crystal structure of MmpL3 and silicon analogues as pharmacophore model also strongly correlate with the biological assays and suggest that the incorporation of silicon in the inhibitor scaffold could enhance their potency by stabilizing the hydrophobic residues at the binding pocket. The best docking hit, compound 12 showed an MIC of 0.1 μM against H37Rv with an acceptable in vitro ADME profile and excellent selectivity index. Overall, the present study indicates that, the designed silicon analogues, especially compound 12 could be a good inhibitor for an intrinsically flexible drug-binding pocket of MmpL3 and has potential for further development as anti-tubercular agents. [Display omitted] • Several analogues of BM212 with silicon incorporation were synthesized and tested for their anti-tubercular activity. • From SAR analysis, silicon substitution improved the potency of this series. • Docking studies suggests that silyl group enhances the potency by stabilizing hydrophobic residues at the binding pocket. • The best docking hit, compound 12 showed an MIC of 0.1 μM against H37Rv and displayed an acceptable in vitro ADME profile. [ABSTRACT FROM AUTHOR]
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- 2023
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10. Synthesis of isomeric corniculatolides
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Raut, Gajanan N., Chakraborty, Kasturi, Verma, Priyanka, Gokhale, Rajesh S., and Srinivasa Reddy, D.
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MACROLIDE antibiotics , *CHEMICAL synthesis , *MOLECULAR structure , *NATURAL products , *ISOMERS , *ANTITUBERCULAR agents , *DRUG synergism - Abstract
Abstract: Synthesis of three natural macrolides 11-O-methylcorniculatolide A, 11-O-methylisocorniculatolide A and isocorniculatolide A is reported using a simple, straight forward and high-yielding route. The present synthesis confirms the assigned molecular structures and provides an access to sufficient quantities of the natural products for the biological evaluation. In addition, we have determined the anti-TB potential of the three natural compounds using Alamar-Blue assay (H37Rv) and found no significant inhibitory activity at 100μg/ml. Excellent yields, short sequence and useful SAR information are the highlights of the present work. [Copyright &y& Elsevier]
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- 2012
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11. Retrobiosynthetic Approach Delineates the Biosynthetic Pathway and the Structure of the Acyl Chain of Mycobacterial Glycopeptidolipids.
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Vats, Archana, Singh, Anil Kumar, Mukherjee, Raju, Chopra, Tarun, Ravindran, Madhu Sudhan, Mohanty, Debasisa, Chatterji, Dipankar, Reyrat, Jean-Marc, and Gokhale, Rajesh S.
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GLYCOPEPTIDES , *BIOSYNTHESIS , *CELL membranes , *TUBERCULOSIS patients , *ACYL carrier protein , *ACTINOMYCETALES - Abstract
Glycopeptidolipids (GPLs) are dominant cell surface molecules present in several non-tuberculous and opportunistic mycobacterial species. GPLs from Mycobacterium smegmatis are composed of a lipopeptide core unit consisting of a modified C26-C34 fatty acyl chain that is linked to a tetrapeptide (Phe- Thr-Ala-alaninol). The hydroxyl groups of threonine and terminal alaninol are further modified by glycosylations. Although chemical structures have been reported for 16 GPLs from diverse mycobacteria, there is still ambiguity in identifying the exact position of the hydroxyl group on the fatty acyl chain. Moreover, the enzymes involved in the biosynthesis of the fatty acyl component are unknown. In this study we show that a bimodular polyketide synthase in conjunction with a fatty acyl- AMP ligase dictates the synthesis of fatty acyl chain of GPL. Based on genetic, biochemical, and structural investigations, we determine that the hydroxyl group is present at the C-5 position of the fatty acyl component. Our retrobiosynthetic approach has provided a means to understand the biosynthesis of GPLs and also resolve the long-standing debate on the accurate structure of mycobacterial GPLs. [ABSTRACT FROM AUTHOR]
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- 2012
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12. Protein Model Discrimination Using Mutational Sensitivity Derived from Deep Sequencing
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Adkar, Bharat V., Tripathi, Arti, Sahoo, Anusmita, Bajaj, Kanika, Goswami, Devrishi, Chakrabarti, Purbani, Swarnkar, Mohit K., Gokhale, Rajesh S., and Varadarajan, Raghavan
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PROTEIN structure , *GENETIC mutation , *PREDICTION models , *BACTERIAL toxins , *BINDING sites , *PHENOTYPES , *MONOMERS - Abstract
Summary: A major bottleneck in protein structure prediction is the selection of correct models from a pool of decoys. Relative activities of ∼1,200 individual single-site mutants in a saturation library of the bacterial toxin CcdB were estimated by determining their relative populations using deep sequencing. This phenotypic information was used to define an empirical score for each residue (RankScore), which correlated with the residue depth, and identify active-site residues. Using these correlations, ∼98% of correct models of CcdB (RMSD ≤ 4Å) were identified from a large set of decoys. The model-discrimination methodology was further validated on eleven different monomeric proteins using simulated RankScore values. The methodology is also a rapid, accurate way to obtain relative activities of each mutant in a large pool and derive sequence-structure-function relationships without protein isolation or characterization. It can be applied to any system in which mutational effects can be monitored by a phenotypic readout. [ABSTRACT FROM AUTHOR]
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- 2012
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13. HLA Alleles and Amino-Acid Signatures of the Peptide-Binding Pockets of HLA Molecules in Vitiligo.
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Singh, Archana, Sharma, Pankaj, Kar, Hemanta K, Sharma, Vinod K, Tembhre, Manoj K, Gupta, Somesh, Laddha, Naresh C, Dwivedi, Mitesh, Begum, Rasheedunnisa, Gokhale, Rajesh S, and Rani, Rajni
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VITILIGO , *MELANOCYTES , *ETIOLOGY of diseases , *AUTOIMMUNITY , *IMMUNOLOGIC diseases , *GENE frequency , *GENETICS , *DIAGNOSIS - Abstract
Vitiligo is a depigmenting disorder of the skin that is characterized by the loss of functional melanocytes from the lesional sites. Although the exact etiology is not understood, autoimmunity is thought to be a crucial deterministic factor. A recurring theme of several autoimmune disorders is the aberrant presentation of self-antigens to the immune system, which triggers downstream perturbations. Here we examine the role of alleles of HLA class I and class II loci to delineate vitiligo manifestation in two distinct populations. Our studies have identified three specific alleles, HLA-A*33:01, HLA-B*44:03, and HLA-DRB1*07:01, to be significantly increased in vitiligo patients as compared with controls in both the initial study on North Indians (N=1,404) and the replication study in Gujarat (N=355) cases, establishing their positive association with vitiligo. Both generalized and localized vitiligo have the same predisposing major histocompatibility complex alleles, i.e., B*44:03 and DRB1*07:01, in both the populations studied, beside the differences in the frequencies of other alleles, suggesting that localized vitiligo too may be an autoimmune disorder. Significant differences in the amino-acid signatures of the peptide-binding pockets of HLA-A and HLA-B α-chain and HLA-DR β-chain were observed between vitiligo patients and unaffected controls. [ABSTRACT FROM AUTHOR]
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- 2012
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14. Transcriptional Upregulation of Nrf2-Dependent Phase II Detoxification Genes in the Involved Epidermis of Vitiligo Vulgaris.
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Natarajan, Vivek T., Singh, Archana, Kumar, Avinash A., Sharma, Pankaj, Kar, Hemanta K., Marrot, Laurent, Meunier, Jean-Roch, Natarajan, Krishnamurthy, Rani, Rajni, and Gokhale, Rajesh S.
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DETOXIFICATION (Alternative medicine) , *EPIDERMIS , *OXIDATIVE stress , *APOPTOSIS , *KERATINOCYTES - Abstract
Oxidative stress is widely believed to be a contributing factor in vitiligo pathogenesis. To explore mechanisms by which epidermis responds to mounting oxidative stress, we investigated the involvement of phase II detoxification genes in vitiligo. Phase II detoxification pathways have recently been identified as being important in the regulation of epidermal skin homeostasis. In this study we show that the key transcription factor nuclear factor E2-related factor 2 (Nrf2) and the downstream genes NAD(P)H:quinone oxidase-1 (NQO-1), γ-glutamyl cystine ligase catalytic subunit (GCLC), and γ-glutamyl cystine ligase modifying subunit (GCLM) are upregulated in the lesional epidermal skin of subjects with vitiligo vulgaris. The differences between lesional and nonlesional skin were further investigated by studying the induced expression of Nrf2-dependent transcripts in skin punch biopsies using curcumin and santalol. Surprisingly, nonlesional skin showed induction of all transcripts while a similar effect was not observed for the skin punches from the lesional skin. The use of curcumin and santalol on epidermal cells showed that keratinocytes were more susceptible to apoptosis, whereas melanocytes induced phase II genes under the same concentrations with negligible apoptosis. Our studies provide new insights into the role of phase II detoxification pathway in maintaining skin homeostasis and sustaining redox balance in vitiligo patients. [ABSTRACT FROM AUTHOR]
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- 2010
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15. A New Family of Type III Polyketide Synthases in Mycobacterium tuberculosis.
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Saxena, Priti, Yadav, Gitanjali, Mohanty, Debasisa, and Gokhale, Rajesh S.
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ENZYMES , *POLYKETIDES , *MYCOBACTERIUM tuberculosis - Abstract
The Mycobacterium tuberculosis genome has revealed a remarkable array of polyketide synthases (PKSs); however, no polyketide product has been isolated thus far. Most of the PKS genes have been implicated in the biosynthesis of complex lipids. We report here the characterization of two novel type III PKSs from M. tuberculosis that are involved in the biosynthesis of long-chain α-pyrones. Measurement of steady-state kinetic parameters demonstrated that the catalytic efficiency of PKS18 protein was severalfold higher for long-chain acyl-coenzyme A substrates as compared with the smallchain precursors. The specificity of PKS18 and PKS11 proteins toward long-chain aliphatic acyl-coenzyme A (C[sub 12] to C[sub 20]) substrates is unprecedented in the chalcone synthase (CHS) family of condensing enzymes. Based on comparative modeling studies, we propose that these proteins might have evolved by fusing the catalytic machinery of CHS and β-ketoacyl synthases, the two evolutionarily related members with conserved thiolase fold. The mechanistic and structural importance of several active site residues, as predicted by our structural model, was investigated by performing site-directed mutagenesis. The functional identification of diverse catalytic activity in mycobacterial type III PKSs provide a fascinating example of metabolite divergence in CHSlike proteins. [ABSTRACT FROM AUTHOR]
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- 2003
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16. 207-P Functional implications of MHC associations in vitiligo
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Rani, Rajni, Singh, Archana, Sharma, Pankaj, Kar, Hemanta K., Sharma, Vinod K., Tembhre, Manoj K., Gupta, Somesh, Laddha, Naresh C., Dwivedi, Mitesh, Begum, Rasheedunnisa, and Gokhale, Rajesh S.
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- 2011
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