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Abstract: Preliminary results of 53Mn measurements for seven Brazilian haematites are presented. The production rate of 53Mn due to cosmic-ray induced disintegration of iron is estimated to be 103±19 atoms g(Fe)-1 yr−1 at sea level and high latitude. This is consistent with the only previously published measurement. The muon contribution to the total 53Mn production at the surface is estimated to be ∼7%. Cosmogenic-isotope dating employing 53Mn is applicable to any rock/mineral type that contains iron as a major constituent, such as haematite, goethite, pyroxene and olivine. The method has the potential to extend the time scale of cosmogenic exposure dating to >10Ma. [Copyright &y& Elsevier]

Abstract: The present study was designed to clarify an intensity-dependent effect of prenatal stress on the morphological development of hippocampal neurons in rats. In addition, the involvement of receptors for glucocorticoids, i.e. mineralocorticoid receptors and glucocorticoid receptors, in stress-induced changes in the morphology of hippocampal neurons was examined by an in vitro pharmacological approach. The effects of mild prenatal stress on neurogenesis and long-term potentiation in the hippocampus were also investigated in adult offspring. Prenatal stress affected the morphological development of the hippocampus in an intensity-dependent manner. Short-lasting, mild prenatal stress enhanced neonatal neurogenesis and differentiation of processes of hippocampal neurons, whereas long-lasting, severe stress impaired their morphology. Mineralocorticoid receptor was found to mediate enhancement of neurogenesis and differentiation of processes of cultured hippocampal neurons. In contrast, glucocorticoid receptor was involved in the suppression of their morphology. Short-lasting, mild prenatal stress, which has previously been shown to enhance learning performance in adult offspring, facilitated neurogenesis and long-term potentiation in the adult hippocampus. These findings suggest that prenatal stress has enhancing and suppressing effects on the development of hippocampal neurons depending on intensity, and that mineralocorticoid receptors and glucocorticoid receptors contribute to stress-induced morphological changes. [Copyright &y& Elsevier]

This study investigates whether maternal stress during pregnancy induces maternal and fetal hypothalamic paraventricular nucleus (PVN) neuronal activation and the effects of maternal stress on fetal hypothalamic and PVN brain-derived neurotrophic factor (BDNF) expression. Pregnant rats were exposed to three types of maternal stress with varying severity (restraint, forced walking and immobilization) for 30 min on gestational day 21. Severity of stress was assessed by measurement of maternal plasma corticosterone 30 min following the stimulus. Maternal plasma corticosterone increased in each stress response group (immobilization>forced walking>restraint). Further, the expression of Fos protein, a marker of neuronal activation, increased in the fetal and maternal PVN in direct relation to the severity of stress treatments. Forced walking and immobilized stress, but not restraint stress, significantly increased BDNF expression in the fetal hypothalamus.These findings suggest that the fetal hypothalamic–pituitary–adrenal (HPA) response following maternal stress mirrors maternal HPA activation. In addition, BDNF may play a role in protecting fetal brain neurons from damage caused by severe stress. [Copyright &y& Elsevier]

We studied the auditory evoked magnetic fields (AEFs) in response to pure tones especially at very high frequencies (from 4000 Hz to 40 000 Hz). This is the first systematic study of AEFs using tones above 5000 Hz, the upper audible range of humans, and ultrasound. We performed two experiments. In the first, AEFs were recorded in 12 subjects from both hemispheres under binaural listening conditions. Six types of auditory stimulus (pure tones of five different frequencies: 4000 Hz, 8000 Hz, 10 000 Hz, 12 000 Hz, 14 000 Hz, and a click sound as the target stimulus) were used. In the second experiment, we used 1000 Hz, 15 000 Hz, and two ultrasounds with frequencies of 20 000 Hz and 40 000 Hz. The subjects could detect all stimuli in the first experiment but not the ultrasounds in the second experiment.We analyzed N1m, the main response with approximately 100 ms in peak latency, and made the following findings. (1) N1m responses to the tones up to 12 000 Hz were clearly recorded from at least one hemisphere in all 12 subjects. N1m for 14 000 Hz was identified in at least one hemisphere in 10 subjects, and in both hemispheres in six subjects. No significant response could be identified to ultrasounds over 20 000 Hz. (2) The amplitude of the N1m to the tones above 8000 Hz was significantly smaller than that to 4000 Hz in both hemispheres. There was a tendency for the peak latency of the N1m to be longer for the tones with higher frequencies, but no significant change was found. (3) The equivalent current dipole (ECD) of the N1m was located in the auditory cortex. There was a tendency for the ECD for the tones with higher frequencies to lie in more medial and posterior areas, but no significant change was found. (4) As for the interhemispheric difference, the N1m amplitude for all frequency tones was significantly larger and the ECDs were estimated to be located more anterior and medial in the right hemisphere than the left. The priority of the right hemisphere, that is the larger amplitude, for very high frequency tones was confirmed. (5) The orientation of the ECD in the left hemisphere became significantly more vertical the higher the tones. This result was consistent with previous studies which revealed the sensitivity of the frequency difference in the left hemisphere.From these findings we suggest that tonotopy in the auditory cortex exists up to the upper limit of audible range within the small area, where the directly air-conducted ultrasounds are not reflected. [Copyright &y& Elsevier]

This study investigated the relationship between the drug combinations of the eradication regimens and the prevalence of acquired resistance to clarithromycin. Of 540 patients treated with anti-Helicobacter pylori regimens containing clarithromycin, 55 patients (31 males, mean age, 45.6 years) with failed eradication of H. pylori that was susceptible to clarithromycin before treatment were included. The E test was used to test for susceptibility to clarithromycin (minimum inhibitory concentration (MIC) <1 mg/l). Of the 55 patients, 33 (60.0%) developed clarithromycin resistance after failed eradication. Of the dual therapies, the combination of a proton pump inhibitor (PPI) and clarithromycin resulted in 88.9% (8/9) of the patients acquiring clarithromycic-resistance. With the triple therapies, the percentages of patients acquiring clarithromycin resistant strains after using a PPI+clarithromycin+amoxycillin or a PPI+clarithromycin+metronidazole were 38.7% (12/31) and 90.0% (9/10), respectively (P<0.01). These data suggest that regimens containing amoxycillin may prevent the selection of secondary clarithromycin resistance. [Copyright &y& Elsevier]

A comparison of the state of 3-D micro-damage induced by hydrogen redistribution due to thermal treatment and deformation near an intergranular fracture surface is carried out in high strength aluminium alloy using synchrotron microtomography. The mapping of various types of micro-damage provides evidence that hydrogen redistribution occurs along with a change in content and shape of micro-pores. The presence of intergranular cracks and an extended agglomerated micro-pore damage state are found to be in close proximity to each other, suggesting a mechanistic relationship for both formation of the unique damage architecture and insidious manifestation of embrittlement in high strength aluminium alloys. [ABSTRACT FROM AUTHOR]

Quantitative tomography is carried out on datasets derived from tensile fracture sample of electrochemically precharged Al-Zn-Mg-Cu alloy in the underaged condition and its uncharged counterpart. It is shown that precharging which induces a transition of tensile fracture mode from ductile to brittle, results in a significant increase in micro-damage content in the regions near the fracture surfaces. Using quantitative tomography analysis based on spatial mapping of morphologically segmented micro-damage content of the datasets it is found that the precharged sample contains an inhomogenous distribution of micro-pores near grain boundaries. It is also shown that the spatial architecture of micro-pores in the dataset is not influenced by the plastic zone of the intergranular cracks lying along the grain boundaries. Contrastingly the micro-pores in the tomographic dataset of the uncharged sample are shown to be present near intermetallic particles. It is therefore rationalized that the spatial architecture of micro-pores in the datasets from uncharged sample originate from particle cracking during ductile fracture, and from the tendency for damage enhancement by the synergism of hydrogen exposure near grain boundaries and localization of deformation in the precharged sample dataset. [ABSTRACT FROM AUTHOR]

• We resolve the enigma of intertwined active and relict gorges in the Finke River. • Flexural response to cyclical tectonic loading imposes to-and-fro topographic tilting. • Loading variations may reflect mantle-flow tractions at the base of the plate. • Multi-million year storage of gorge gravels signifies the timescale of tilting. The Finke River in central Australia is counted among the world's oldest drainage systems; this raises the prospect of investigating how (tectonic and sub-lithospheric) processes have shaped the landscape in the long term. The Finke's upper reaches display an enigmatic set of intertwined active and relict bedrock gorges that suggest a complex history of incision, aggradation and re-incision. We measured cosmogenic 10Be and 26Al in fluvial gravels preserved in the gorges, and we applied a Markov chain-Monte Carlo-based inversion model to test two limiting-case hypotheses about the timing of gravel deposition and exhumation. Our results suggest that the nuclide memory contained within the gravels was essentially erased during sediment storage exceeding several million years. Previous studies attribute central Australia's geomorphic development to intensified post-Miocene aridity in tune with the perception of a quiescent tectonic regime. However, the close correlation between drainage patterns and gravity anomalies leads us to propose, instead, that cyclical in-plane loading in the presence of extreme lithospheric density anomalies caused to-and-fro tilting of the upper Finke. We show that relief variations of order 100 m accompany mild in-plane loading of ∼1–2 x 1012 N m−1, and we speculate that loading variations are likely the result of tractions imposed at the base of the plate rather than at plate boundaries. Acting together, these tectonic and sub-lithospheric processes triggered the phases of incision and aggradation that created the Finke's unusual intertwined bedrock gorges over a multi-million-year timescale. [ABSTRACT FROM AUTHOR]

Abstract: The present status of the AMS activities at the Australian National University is described. Particular attention is paid to methods and equipment that have evolved substantially since earlier reports. [Copyright &y& Elsevier]

There is growing interest in geochronological applications of terrestrial in situ -produced cosmogenic nuclides, with the most commonly measured being 10Be and 26Al in quartz. To extract and then separate these radionuclides from quartz and prepare them in the oxide form suitable for accelerator mass spectrometry (AMS) requires extensive and careful laboratory processing. Here we discuss the suitability of a crushed, sieved and etched, sub-aerially exposed vein quartz specimen (CoQtz-N) to act as a reference material for chemical laboratory preparation and AMS measurements. Splits of CoQtz-N were distributed to eleven target preparation laboratories. The CoQtz-N 10Be targets were then measured at seven different AMS facilities and five of the preparation laboratories had their 26Al targets measured at four different AMS facilities. We show that CoQtz-N splits are sufficiently homogeneous with regard to nuclide concentrations, that it has been cleaned of any atmospheric derived (i.e. meteoric) 10Be and that it has low concentrations of the major elements that can interfere with Be and Al extraction chemistry and AMS measurements. We derive preliminary concentrations for 10Be and 26Al in CoQtz-N as 2.53 ± 0.09 × 106 at/g and 15.6 ± 1.6 × 106 at/g, respectively, at the 95% confidence limit. [ABSTRACT FROM AUTHOR]

Background: S-288310, a cancer peptide vaccine composed of two HLA-A*24:02-restricted peptides derived from two oncoantigens, DEP domain-containing 1 (DEPDC1) and M-phase phosphoprotein 1 (MPHOSPH1), was investigated in urothelial carcinoma (UC) of the bladder. Patients and methods: Thirty eight HLA-A*24:02-positive patients with progressive UC were enrolled in this study. In the phase I part of the study, three patients each were treated with S-288310 at 1 mg or 2 mg/peptide subcutaneously once a week to evaluate safety and tolerability. In the phase II, 32 patients were randomized to receive either 1 mg or 2 mg to evaluate the difference in cytotoxic T lymphocytes (CTL) induction and safety. Results: S-288310 was safe and well tolerated in the phase I. Of 27 patients evaluable for immune responses in the phase II, there was no difference in CTL induction rate between the 1 mg (100%) and 2 mg (80.0%) groups. Of 32 patients receiving S-288310 in the phase II, the most frequent drug-related AE was the injection site reaction that was observed in 29 patients (90.6%), but none of the patients discontinued administration due to these reactions and no dose relationship in the frequency and severity was observed. The objective response rate of the 32 patients was 6.3% and the disease control rate was 56.3%. The median overall survival (OS) rates for patients vaccinated with S-288310 after one regimen of chemotherapy, 2 regimens, or 3 or more were 14.4, 9.1 and 3.7 months, respectively, and 32.2% of patients post first-line treatment were alive at 2 years. OS of patients who showed CTL induction to both peptides was longer than that of those with CTL induction to no or one peptide. Conclusion: S-288310 was well-tolerated and effectively induced peptide-specific CTLs, which were correlated with longer survival for patients with UC of the bladder. [ABSTRACT FROM AUTHOR]

Abstract: Intraplate tectonism has produced large-scale folding that steers regional drainage systems, such as the 1600km-long Cooper Ck, en route to Australia''s continental depocentre at Lake Eyre. We apply cosmogenic 10Be exposure dating in bedrock, and luminescence dating in sediment, to quantify the erosional and depositional response of Cooper Ck where it incises the rising Innamincka Dome. The detachment of bedrock joint-blocks during extreme floods governs the minimum rate of incision (17.4±6.5mm/ky) estimated using a numerical model of episodic erosion calibrated with our 10Be measurements. The last big-flood phase occurred no earlier than ∼112–121ka. Upstream of the Innamincka Dome long-term rates of alluvial deposition, partly reflecting synclinal-basin subsidence, are estimated from 47 luminescence dates in sediments accumulated since ∼270ka. Sequestration of sediment in subsiding basins such as these may account for the lack of Quaternary accumulation in Lake Eyre, and moreover suggests that notions of a single primary depocentre at base-level may poorly represent lowland, arid-zone rivers. Over the period ∼75–55ka Cooper Ck changed from a bedload-dominant, laterally-active meandering river to a muddy anabranching channel network up to 60km wide. We propose that this shift in river pattern was a product of base-level rise linked with the slowly deforming syncline–anticline structure, coupled with a climate-forced reduction in discharge. The uniform valley slope along this subsiding alluvial and rising bedrock system represents an adjustment between the relative rates of deformation and the ability of greatly enhanced flows at times during the Quaternary to incise the rising anticline. Hence, tectonic and climate controls are balanced in the long term. [Copyright &y& Elsevier]

Abstract: A study has been carried out to assess the potential of using fallout plutonium (Pu), which originated from atmospheric nuclear-weapons tests, as a tool to investigate recent erosional processes within the lower Cotter water-supply catchment in the Australian Capital Territory. This catchment, which was predominantly pine plantation, was severely affected by a major bush fire in 2003. Accelerator mass spectrometry has been used to measure Pu in soil samples collected from a number of sites across the catchment. The results indicate that less than 1cm of surface soil had been lost since the early 1960s over much of the catchment. Areas of more erodible soil have, however, lost 2–4cm of topsoil, and a loss of ∼6cm of soil was identified at one particular site. [Copyright &y& Elsevier]

Clin Microbiol Infect 2010; 16: 1264–1269 The dupA gene of Helicobacter pylori was suggested to be a risk factor for duodenal ulcer but protective against gastric cancer. The present study aimed to re-examine the role of dupA in H. pylori-infected Japanese patients. We found that dupA status was not associated with any gastroduodenal disease, histological score of chronic gastritis or with the extent of interleukin-8 production from gastric cell lines. These results indicate that dupA is unlikely to be a virulence factor of H. pylori in the Japanese population. [ABSTRACT FROM AUTHOR]

Abstract: We have developed a novel, compact-mode selective annular-beam generator that employs a laser-diode (LD)-pumped, passively locked power build-up cavity (PBC). The PBC functions as a flexible and compact light source that is capable of producing TEM0n high-order Hermite-Gaussian (HG) beams. The generated HG beams can then be converted to LG modes, particularly to annular-beams, using two cylindrical lenses. The system was theoretically studied and a mode-matching condition was derived. The tailoring position of the active semiconductor laser medium relative to the external cavity of the PBC enabled the production of single high-order HG modes. The optimum position was calculated by model calculations of the mode-coupling efficiency. The desired TEM0n modes of HG beams were generated by translating the axial position of the external cavity of the PBC in increments of ∼100μm relative to the LD emission surface. High quality TEM01 to TEM015 of the HG beams and their conversion to the desired LG modes having powers of the order of 1mW was successfully demonstrated. This system is suitable for optical tweezers. [Copyright &y& Elsevier]

Abstract: The effects of repeated prenatal stress with different severity (restraint and immobilization) on Fos expression in the maternal and fetal hypothalamic paraventricular nucleus (PVN) were examined in rats. Acute stress treatment was performed for 30 min on gestational day 21, and repeated stress treatment for 30 min daily for 5 days from gestational days 17–21. In the parvocellular region of the maternal PVN, the stress-induced increases in the number of Fos-immunoreactive neurons were smaller in the repeated stress groups than the acute stress groups, indicating an adaptation of Fos expression to repeated stress. The attenuated Fos expression observed in the maternal PVN following repeated mild stress did not occur in the fetal PVN. In contrast, repeated immobilization stress caused a much smaller increase in Fos expression in the fetal PVN than did acute immobilization stress. The reduced Fos expression in the fetal PVN following repeated severe stress was thought to be due to cell death, since the fetal PVN in the chronic immobilization group revealed a reduction in the total number of cells and an increase in the number of apoptotic cells. In the female but not male fetuses, repeated restraint stress induced a significant increase in the number of apoptotic cells in the PVN. These findings suggest that the fetal PVN shows no adaptation of Fos expression to repeated maternal stress, but great vulnerability to cell death, including apoptosis. In addition, stress-induced apoptosis may more easily occur in the fetal PVN in females than males. [Copyright &y& Elsevier]

Abstract: A new megastigmane diglycoside was isolated from the leaves of Carallia brachiata. The structure was determined by spectroscopic methods as 3-hydroxy-5,6-epoxy-β-ionol -3-O-β-apiofuranosyl-(1→6)-β-glucopyranoside (1). Additionally, 29 known compounds consisting of two megastigmanes, one 1,2-dithiolane derivative, seven aromatic compounds, five condensed tannins, 12 flavonoids, and two glyceroglycolipids were isolated and identified. [Copyright &y& Elsevier]