15 results on '"Finnegan, Patrick M."'
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2. Leaf phosphorus allocation to chemical fractions and its seasonal variation in south-western Australia is a species-dependent trait
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Liu, Shu Tong, Gille, Clément E., Bird, Toby, Ranathunge, Kosala, Finnegan, Patrick M., and Lambers, Hans
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- 2023
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3. Effects of litter mixtures on aerobic decomposition rate and its temperature sensitivity in a boreal peatland
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Zhang, Xinhou, Wang, Xianwei, Finnegan, Patrick M., Tan, Wenwen, and Mao, Rong
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- 2019
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4. An alternative oxidase monoclonal antibody recognises a highly conserved sequence among alternative oxidase subunits
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Finnegan, Patrick M., Wooding, Anthea R., and Day, David A.
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- 1999
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5. How Does Evolution in Phosphorus-Impoverished Landscapes Impact Plant Nitrogen and Sulfur Assimilation?
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Prodhan, M. Asaduzzaman, Finnegan, Patrick M., and Lambers, Hans
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PHOSPHATE fertilizers , *PLANT nitrogen metabolism , *PLANT evolution , *LANDSCAPE ecology , *RIBOSOMAL RNA - Abstract
Phosphorus (P) fertilisers, made from rock phosphate, are used to attain high crop yields. However, rock phosphate is a finite resource and excessive P fertilisers pollute our environment, stressing the need for more P-efficient crops. Some Proteaceae have evolved in extremely P-impoverished environments. One of their adaptations is to curtail the abundance of ribosomal RNA, and thus protein, and tightly control the acquisition and assimilation of nitrogen (N) and sulfur. This differs fundamentally from plants that evolved in environments where N limits plant productivity, but is likely common in many species that evolved in P-impoverished landscapes. Here, we scrutinise the relevance of these responses towards developing P-efficient crops, focusing on plant species where 'P is in the driver's seat'. Highlights Proteaceae grow predominantly across the southern hemisphere on soils that greatly contrast in age and plant-available phosphorus (P). Due to adaptations to a wide range of soil P concentrations, Proteaceae species in habitats with contrasting soil P concentrations have evolved unique adaptive traits that are ecologically relevant, but greatly contrasting across habitats. In severely P-impoverished habitats, 'P is in the driver's seat'. Proteaceae offer a highly relevant and exciting model to unearth P-efficiency traits focusing on the plant species that have evolved in, and are adapted to, low-P environments. The focus of P-efficiency studies in the literature is almost exclusively on plant species that evolved in nitrogen (N)-limited young landscapes. Therefore, the responses of these species to P limitation are predominantly P-starvation responses, rather than P-efficiency mechanisms. We can learn a great deal from studies of species that evolved in severely P-impoverished environments to work towards crops with a decreased demand for P, which is a dwindling and finite natural resource. [ABSTRACT FROM AUTHOR]
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- 2019
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6. Sixth International Congress on Plant Mitochondria: mitochondrial biologists meet in Perth, Australia
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Finnegan, Patrick M.
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- 2003
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7. Mechanisms underlying enhanced Cd translocation and tolerance in roots of Populus euramericana in response to nitrogen fertilization.
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Song, Junyu, Finnegan, Patrick M., Liu, Wenhui, Li, Xiang, Yong, Jean W.H., Xu, Jiuting, Zhang, Qi, Wen, Yuxin, Qin, Kexin, Guo, Jinze, Li, Ting, Zhao, Chang, and Zhang, Yi
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FERTILIZERS , *POPLARS , *GLUTATHIONE reductase , *NITROGEN in soils , *NITROGEN - Abstract
• Cd absorption and accumulation in roots increased under N-deficient condition. • Fine root length was tightly correlated with Cd absorption and Cd accumulation in roots. • Cd uploading in roots and Cd translocation to shoots were decrased by N deficiency. • Genes for Cd detoxification, uploading and antioxidant defense in roots were up-regulated by N application. • N-stimulated Cd detoxification, uploading and antioxidation in roots contributed to Cd translocation. A pot experiment was conducted to evaluate how nitrogen (N) availability influences cadmium (Cd) absorption, translocation and stress tolerance in roots of Populus euramericana. Seedling growth was sensitive to N deficiency, but it was unaltered by Cd exposure. Cadmium absorption by roots was promoted by N deficiency, resulting in a higher root Cd concentration compared to the N-sufficient condition. Fine-root length was tightly correlated (R2 = 0.73) with Cd concentration in roots, indicating that vigorous fine-root proliferation under N deficiency contributed to active absorption and accumulation of Cd in roots. Despite accumulation in roots, Cd translocation from roots to shoots was less active under N deficiency compared to N sufficiency. This was related to elevated glutathione reductase (GR) activity and glutathione (GSH) levels in roots after N application, which may not only promote antioxidant defence, but also facilitate the formation of GSH–Cd complexes that are uploaded into root cylinders. Nitrogen application also promoted antioxidant defense in roots via increased production of phytohormones and the level of enzymatic and non-enzymatic antioxidants. Transcript levels for genes responsible for antioxidant defense, Cd detoxification and Cd uploading were increased in roots by N application. The N-stimulated Cd tolerance, detoxification and uploading in roots are factors likely to promote Cd translocation from roots to shoots, which may enhance the biological capacity of this poplar species for phytoremediation of Cd pollution. [ABSTRACT FROM AUTHOR]
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- 2019
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8. The mature AEP2 gene product of Saccharomyces cerevisiae, required for the expression of subunit 9 of ATP synthase, is a 58 kDa mitochondrial protein
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Finnegan, Patrick M., Ellis, Timothy P., Nagley, Phillip, and Lukins, H. Bruce
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- 1995
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9. New sources of resistance to Sclerotinia sclerotiorum for crucifer crops.
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Uloth, Margaret B., You, Ming Pei, Finnegan, Patrick M., Banga, Surinder S., Banga, Shashi K., Sandhu, Prabhjot S., Yi, Huang, Salisbury, Phillip A., and Barbetti, Martin J.
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SCLEROTINIA sclerotiorum , *BRASSICACEAE , *DISEASE resistance of plants , *PLANT stems , *PLANT genetics , *COMPOSITION of leaves - Abstract
Highlights: [•] High-level resistance to Sclerotina identified across diverse cruciferous spp. [•] Stem and leaf resistance are under separate genetic control. [•] Species value as a resistance source depends upon number of genotypes tested. [•] Resistances identified allow new disease-resistant commercial cruciferous crops. [Copyright &y& Elsevier]
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- 2013
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10. Daptomycin Compared to Vancomycin for the Treatment of Osteomyelitis: A Single-Center, Retrospective Cohort Study
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Moenster, Ryan P., Linneman, Travis W., Finnegan, Patrick M., and McDonald, Jay R.
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VANCOMYCIN , *PEPTIDE antibiotics , *BLOOD testing , *CHI-squared test , *COMPARATIVE studies , *LONGITUDINAL method , *OSTEOMYELITIS , *HEALTH outcome assessment , *TREATMENT effectiveness , *RETROSPECTIVE studies , *CASE-control method , *THERAPEUTICS ,DISEASE relapse prevention - Abstract
Abstract: Background: Osteomyelitis (OM) is a serious infection with high rates of recurrence. Vancomycin has been used for decades in the treatment of OM, but, despite adequate dosing, 30% to 50% of patients experience infection recurrence within 12 months. Daptomycin, a novel lipopetide antibiotic, is also active against resistant gram-positive organisms, but there is little published about its efficacy and tolerability in the treatment of OM. Objective: Our aim was to compare the recurrence rates of OM in patients treated with daptomycin or vancomycin. Methods: A retrospective cohort study of all patients at a VA Medical Center between January 1, 2003, and July 31, 2009, who received daptomycin for the treatment of OM was undertaken. Patients with a diagnosis of OM who received at least 2 weeks of daptomycin and had at least 1 follow-up visit within 6 months after completion of therapy were included. Each patient was matched with 2 controls treated with at least 2 weeks of vancomycin for OM. Matching criteria included previous OM, diabetes, peripheral vascular disease, hardware involvement, and surgical therapy. Patients were excluded from the evaluation if they received <14 days of therapy, had no follow-up in the 6 months after therapy was discontinued, had an absolute neutrophil count <500 cells/mm3, or were receiving vancomycin and daptomycin concurrently. The primary outcome was recurrence of infection within 6 months after the discontinuation of therapy. Secondary outcomes included mean change in creatine phosphokinase (CPK), incident thrombocytopenia, and mean doses of antibiotics. The χ2 test was used to compare rates of recurrence between groups. Results: Seventeen patients received at least 2 weeks of daptomycin for the treatment of OM and were matched to 34 vancomycin controls. Twenty-nine percent of patients receiving daptomycin had a recurrence of infection compared with 61.7% in the vancomycin group (P = 0.029). The mean change in CPK for the daptomycin group was +28.8 U/L. No thrombocytopenia developed in any patients receiving daptomycin compared with 2 (5.9%) patients in the vancomycin group. Conclusions: In a limited number of cases, significantly fewer patients treated with daptomycin for OM had a recurrence of their infection. Daptomycin may be a tolerable and effective alternative to vancomycin for the treatment of OM. [Copyright &y& Elsevier]
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- 2012
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11. Development of DNA markers for hybrid identification in Leucadendron (proteaceae)
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Liu, Hui, Yan, Guijun, Finnegan, Patrick M., and Sedgley, Ralph
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NUCLEIC acids , *PLANT propagation , *GENETIC polymorphisms , *ANIMAL variation - Abstract
Abstract: A rapid and reliable method to accurately identify hybrids at an early age is essential to the success of Leucadendron breeding programs because identification based on morphology can be difficult or impossible when the seedlings are young. DNA based PCR-RFLP and random amplified microsatellite polymorphism (RAMP) markers were developed for this purpose. Unexpected non-parental fragments appeared during the PCR-RFLP analysis of the nuclear ITS region of L. uliginosum 05× L. procerum 04 hybrids. Mixing DNA from both parents in a single PCR also produced the non-parental fragment, suggesting that PCR recombination had introduced a novel restriction site into the products from the hybrids. Sequencing of individual amplified ITS products from the hybrids confirmed this conclusion. To avoid this complication, RAMP markers were developed for accurate hybrid identification in Leucadendron. RAMP analysis generated a considerable number of polymorphic products, and showed more discrimination in identifying Leucadendron hybrids than did PCR-RFLP. [Copyright &y& Elsevier]
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- 2007
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12. A tomato alternative oxidase protein with altered regulatory properties
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Holtzapffel, Ruth C., Castelli, Joanne, Finnegan, Patrick M., Millar, A. Harvey, Whelan, Jim, and Day, David A.
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PROTEINS , *GENES , *DIMERS , *GENOMES - Abstract
We have investigated the expression and regulatory properties of the two alternative oxidase (Aox) proteins that are expressed in tomato (Lycopersicon esculentum L. Mill cv. Sweetie) after storage of green fruit at 4 °C. Four Aox genes were identified in the tomato genome, of which two (LeAox1a and LeAox1b) were demonstrated to be expressed in cold-treated fruit. The activity and regulatory properties of LeAox1a and LeAox1b were assayed after expression of each protein in yeast cells (Saccharomyces cerevisiae), proving that each is an active Aox protein. The LeAox1b protein was shown to have altered regulatory properties due to the substitution of a Ser for the highly conserved CysI residue. LeAox1b could not form inactive disulfide-linked dimers and was activated by succinate instead of pyruvate. This is the first example of a dicot species expressing a natural CysI/Ser isoform. The implications of the existence and expression of such Aox isoforms is discussed in the light of the hypothesised role for Aox in plant metabolism. [Copyright &y& Elsevier]
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- 2003
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13. Plant functional group controls litter decomposition rate and its temperature sensitivity: An incubation experiment on litters from a boreal peatland in northeast China.
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Mao, Rong, Zhang, Xinhou, Song, Changchun, Wang, Xianwei, and Finnegan, Patrick M.
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PEATLAND ecology , *MICROBIAL biotechnology , *FUNCTIONAL groups , *SHRUBLANDS , *PLANT nutrients - Abstract
In boreal peatlands, litter decomposition plays an important role in modulating ecosystem carbon (C) cycling and nutrient turnover. However, how climate warming and plant functional group interact to affect litter decomposition is still unclear in these ecosystems. Here, we collected fresh litters of six plant functional groups (nitrogen (N)-fixing species, deciduous tree, deciduous shrub, evergreen shrub, graminoid, and Sphagnum moss) from a boreal peatland located in northeast China. A laboratory incubation experiment was used to determine the effect of temperature (10 °C vs. 20 °C) on microbial respiration and mass loss during decomposition. Among the six functional groups, the litters of N-fixing species and deciduous shrub, followed by deciduous tree, generally had the greatest mass losses and microbial respiration rates, whereas the Sphagnum moss decomposed with the slowest rate at both incubation temperatures. Increasing incubation temperature from 10 °C to 20 °C, microbial respiration rate and mass loss increased slightly for Sphagnum moss litters (25% and 19%, respectively), but increased dramatically for vascular plant litters (84–135% and 49–85%, respectively). For litters from vascular plants, both decomposition rate and temperature sensitivity showed a tight linear correlation with the initial C:N and C:phosphorus ratios. Considering that climate warming will cause increased dominance of woody plant species coupled with decreased cover by Sphagnum mosses, this study provides clear evidence that climate warming and the associated changes to vegetation community composition can synergistically accelerate plant litter decomposition in boreal peatlands. [ABSTRACT FROM AUTHOR]
- Published
- 2018
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14. Sugar and dihydroxyacetone ratios in floral nectar suggest continuous exudation and reabsorption in Leptospermum polygalifolium Salisb.
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Obeng-Darko, Sylvester A., Brooks, Peter R., Veneklaas, Erik J., and Finnegan, Patrick M.
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NECTAR , *DIHYDROXYACETONE , *HIGH performance liquid chromatography , *FLOWER development , *SUGAR - Abstract
Leptospermum polygalifolium Salisb. can accumulate high concentrations of dihydroxyacetone (DHA), precursor of the antimicrobial compound methylglyoxal found in honey obtained from floral nectar of Leptospermum spp. Floral nectar dynamics over flower lifespan depends on internal and external factors that invariably impact nectar quality. Current models to estimate nectar quality in Leptospermum spp. overlook time of day, daily (24 h), and long-term dynamics of nectar exudation and accumulation over flower lifespan. To explain the dynamics of nectar quality over flower lifespan, accumulated nectar from flowers of different ages was collected from two L. polygalifolium clones, and then re-collected 24 h later from the same flowers. High-Performance Liquid Chromatography was used to quantify DHA amount and total equivalents of glucose + fructose (Tsugar) per flower in the nectar. DHA and Tsugar amount per flower differed with flower age and between clones. In accumulated nectar, the amount of DHA and Tsugar per flower rose to a broad peak post-anthesis before decreasing. Immediately after peaking DHA declined more quickly than Tsugar in accumulated nectar due to a greater decrease in the exudation of DHA than for Tsugar. The DHA : Tsugar ratios in accumulated nectar and in nectar exuded over the next 24 h were similar and decreased with flower age, indicating that exudation and reabsorption occurred concomitantly across flower development. Hence there is a balance between exudation and reabsorption. A quantitative model suggested that flowers have the potential to exude more DHA and Tsugar than actually accumulated. • Dihydroxyacetone (DHA) and total sugar (Tsugar) in nectar initially increased before declining across flower development. • The amounts of DHA and Tsugar that accumulated were signficantly less than what flowers were able to exude • The changes in DHA: Tsugar ratio during flower life suggested that nectar exudation and reabsorption occurred concomitantly. [ABSTRACT FROM AUTHOR]
- Published
- 2022
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15. Delineation of Sclerotinia sclerotiorum pathotypes using differential resistance responses on Brassica napus and B. juncea genotypes enables identification of resistance to prevailing pathotypes
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Ge, Xin Tian, Li, Yu Pin, Wan, Zhi Jian, You, Ming Pei, Finnegan, Patrick M., Banga, Surinder S., Sandhu, Prabhjot S., Garg, Harsh, Salisbury, Phillip A., and Barbetti, Martin J.
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RUTABAGA , *DISEASE resistance of plants , *PLANT genetics , *SCLEROTINIA sclerotiorum , *RAPE (Plant) , *PLANT growth , *EXPERIMENTAL agriculture , *PLANT breeding - Abstract
Abstract: Sclerotinia stem rot caused by the fungus Sclerotinia sclerotiorum is one of the most damaging and difficult-to-manage diseases of oilseed rape (Brassica napus) and mustard (B. juncea). Identifying oilseed Brassica genotypes with effective resistance offers the best long-term prospect for improved management of the disease. Despite some significant interactions between oilseed Brassica genotypes and S. sclerotiorum isolates reported in earlier studies, mostly a single pathogen isolate has been used to identify resistant genotypes. This paper reports the results of studies involving 53 isolates of S. sclerotiorum from the northern and southern agricultural regions of Western Australia where Sclerotinia stem rot is a serious disease of oilseed rape. Colony characteristics of isolates on potato dextrose agar were determined, and two field virulence (i.e., levels of disease severity) studies conducted. The first field study included 14 Brassica genotypes against three S. sclerotinia isolates and the second had eight Brassica genotypes against 50 S. sclerotinia isolates. Only colony diameter of isolates growing on potato dextrose agar was correlated with stem lesion length in the field. In both field experiments, there were significant effects of isolates and host genotypes, as well as a significant interaction between isolates and genotypes in relation to stem lesion length. In the first field experiment, mean stem lesion length ranged from 1cm in the highly resistant B. napus ZY006 to at least 9cm for susceptible genotypes Brassica juncea #2 and B. juncea Montara. The latter genotype was the most susceptible with a mean stem lesion length of 11.1. Expression of high-level resistance in B. napus ZY006 was largely independent of S. sclerotiorum isolate. In contrast, responses in B. napus genotypes, Zhongyou 821, 06-6-3792, RT108 and Charlton were much more isolate-dependent. In the first field experiment, with a greater number of host genotypes than S. sclerotiorum isolates, a higher variance ratio occurred for isolates (VR=158.4) than for host genotypes (VR=10.7). In the second field experiment, with a larger number of S. sclerotinia isolates than hosts, host genotypes had a higher variance ratio (VR=458.9) compared to the isolates (VR=71.2). Increasing the number of isolates greatly improved the capacity to differentiate levels of resistance among test genotypes. In this experiment, some genotypes showed more consistent resistant reactions (e.g., B. napus Mystic and B. juncea Xinyou 9) across different isolates and these are ideal targets for commercial exploitation of this resistance in oilseed Brassica breeding programs. From this experiment, a standardized set of three B. napus and three B. juncea genotypes were shown to be suitable for use as universal differentials to characterize pathotypes of S. sclerotiorum using octal nomenclature. Eight distinct pathotypes of S. sclerotiorum were delineated and characterized and these six host differential genotypes can now be utilized to identify and monitor the incidence and distribution of current and future pathotypes of S. sclerotiorum. Further, by providing a reliable means to characterize pathotypes of S. sclerotiorum, for the first time not only allows identification of resistance(s) against the predominant pathotype(s) of S. sclerotiorum prevailing in a particular region, but also now allows oilseed rape breeding programs to combine host resistances against several specific pathotypes of S. sclerotiorum into future cultivars. [Copyright &y& Elsevier]
- Published
- 2012
- Full Text
- View/download PDF
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