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The banana is very popular globally for its flavour, nutrition, and economic value. However, slow regeneration from suckers, and diseases, there is shortfall of clean planting materials. Though, the in vitro micropropagation is effective for rapid production of planting materials, but due to high cost, the small-scale poor farmers unable to afford. The macropropagation technique offers an alternative and cost effective way to produce planting materials. This study present a simple, efficient and cost efficient macropropagation protocol of banana for formulation of effective potting mix. The experimental design was based on different corm/pseudostem splitting technique and different combinations of potting mix substrata. The overall optimum response in macropropagation techniques was found in PIF (T 3) and Split corm + PIF method (T 5) and Forest soil (P 3) and Saw dust + Sand (P 5). For the study banana cultivar Grand Nain (G9) was taken as a model plant. The regenerated plantlets was assessed for genetic stability using two markers i.e., RAPD and SCoT and found that the regenerates were 97.37–100 % genetically stable. Protocol developed is simple, reproducible, cost effective and sustainable and can be followed by the unskilled farmers. Though, in this study the G9 cultivar was used as model, the technique can be effectively used for other banana cultivars. [Display omitted] • Formulation of organic potting mix for macropropagation of banana using G9 cultivar as model. • Optimized the corms splitting technique for efficient shoot regeneration. • The unskilled farmers can follow the protocol and produced their clonal planting materials for small to medium-scale farming. • Regenerates establishes easily in the cultivation plot. • Genetic stability assessments indicate 97.37–100 % genetically stable regenerated plantlets. [ABSTRACT FROM AUTHOR]