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Short- or mid-term fasting, full or partial, triggers metabolic response known to have in turn health effects in an organism. At central level, the metabolic stimulus triggered by fasting is known to be perceived firstly by hypothalamic neurons. In the field of neuroscience, ribosomal protein S6 (S6) phosphorylation is commonly used as a readout of the mammalian target of rapamycin complex 1 signalling activation or as a marker for neuronal activity. The aim of this study is addressed to evaluate whether the phosphorylation of S6 occurs in the central neurons of zebrafish exposed to four (short-term) and seven (mid-term) days of complete fasting. Group-housed adult zebrafish were exposed to four and seven days of complete food withdrawal. At the end of the experimental period, Western blotting analyses were carried out to measure the expression levels of the phosphorylated S6 (pS6) by comparing the two experimental conditions versus the control group. The same antibody was then used to identify the distribution pattern of pS6 immunoreactive neurons in the whole brain and in the taste buds. We did not observe increased pS6 levels expression in the brain of animals exposed to short-term fasting compared to the control, whereas the expression increased in brain homogenates of animals exposed to mid-term fasting. pS6 immunoreactivity was reported in some hypothalamic neurons, as well as in the dorsal area of telencephalon and preoptic area, a neurosecretory region homolog to the mammalian paraventricular nucleus. Remarkably, we observed pS6 immunostaining in the sensory cells of taste buds lining the oral epithelium. Taken together, our data show that in zebrafish, differently from other fish species, seven days of fasting triggers neuronal activity. Furthermore, the immunostaining on sensory cells of taste buds suggests that metabolic changes may modulate also peripheral sensory cells. This event may have valuable implications when using zebrafish to design metabolic studies involving fasting as well as practical consequences on the animal welfare, in particularly stressful conditions, such as transportation. [ABSTRACT FROM AUTHOR]

Purpose: To compare graft coronal and sagittal inclination angles in anterior cruciate ligament (ACL) reconstruction performed by different femoral tunnel drilling techniques with respect to intact native ACL.Methods: In total, 72 patients were prospectively enrolled in the study. The inclusion criteria were complete ACL rupture and patient age between 18 and 55 years. Reconstructions were performed using 4 different femoral tunnel drilling technique: transtibial (TT), anteromedial portal with rigid (AMP-RR) or flexible (AMP-FR) reamer, and outside-in retrograde drilling (OI) techniques. Eighteen patients with intact native ACL were included as controls. Sagittal and coronal graft inclination angles were measured by magnetic resonance imaging 6 months after the procedure by 1 radiologist blinded in regards to the used technique.Results: OI and AMP-FR techniques allowed for the maintenance of native-like ACL inclination in both the sagittal and coronal planes, whereas TT and AMP-RR increased the sagittal angle by a mean of 9.5° (P < .001) and 6.7° (P = .003), respectively, compared with native ACLs. AMP-RR and TT also showed increased sagittal graft inclination compared with AMP-FR (+6.1°, P = .009 and +9.0°, P < .001, respectively) and OI-drilling techniques (+5.5°, P = .024 and +8.4°, P < .001, respectively). No differences were observed among study groups in terms of coronal graft inclination.Conclusions: The study hypothesis was partially confirmed, since OI and AMP-FR techniques, but not AMP-RR, using an independent portal for femoral drilling produce a more anatomic graft inclination on the sagittal plane with respect to TT.Level Of Evidence: II, prospective comparative study. [ABSTRACT FROM AUTHOR]

Purpose: The aim of this retrospective study was to investigate, at 8 years, the clinical follow-up and failure rate (revision rate/conversion to arthroplasty) of patients with hip chondral lesions associated with femoroacetabular impingement and to compare over time the treatment by microfracture (MFx) and autologous matrix-induced chondrogenesis (AMIC).Methods: Patients aged between 18 and 55 years, with acetabular grade III and IV chondral lesions (Outerbridge), measuring 2 to 8 cm2 operated on at least 8 years before enrollment. Exclusion criteria were rheumatoid arthritis, dysplasia, or axial deviation of the femoral head. There were no arthritic lesions, Tonnis < 2, or joint space of at least 2 mm. MFx was performed with an awl, and the Chondro-Gide membrane used for the AMIC procedure was placed without glue. Outcomes used modified Harris hip score (mHHS) at 6 months and yearly for 8 years and patient acceptable symptomatic state.Results: Among 130 patients, 109 fulfilled inclusion criteria. Fifty were treated by MFx and 59 by AMIC. The mHHS significantly improved in both groups from 46 ± 6.0 to 78 ± 8.8 for mHHS at 6-12 months, even for lesions > 4 cm2. From 2 to 8 years, mHHS in the AMIC group was better than in the MFx group (P < .005). This mHHS improvement in the AMIC group was maintained through the 8-year follow-up period, whereas it deteriorated after 1 year in the MFx group (P < .005). Eleven patients (22%) in the MFx group required total hip arthroplasty (THA); none in the AMIC group did. Patient acceptable symptomatic state analysis confirmed similar short-term improvement, but a significant (P < .007) degradation after 2-8 years in MFx patients.Conclusions: MFx and AMIC techniques led to marked clinical short-term improvement in patients with chondral defects resulting from femoroacetabular impingement in the first 2 years. However, AMIC gave significantly better results as measured by mHHS, which were maintained after 8 years, the results of MFx in the hip deteriorated over time with 22% of patients undergoing conversion to THA. No patient in the AMIC group was converted to THA; the results of AMIC appeared stable over time and independent of lesion size.Level Of Evidence: III, retrospective patient group study. [ABSTRACT FROM AUTHOR]

Abstract In this study, evidence for leptin receptor (LR) and gastric leptin immunoreactivity along the digestive tract of the rainbow trout (Oncorhynchus mykiss), is reported. Besides this, the regulation of gastric leptin and its transcript by fatty acids was analyzed in vitro. LR was detected mainly in the cells of the stomach gastric glands and in the brush border of the epithelium of the anterior, middle and distal intestine. In the stomach LR was co-distributed with leptin. The regulation of gastric leptin and its transcript by fatty acids was analyzed by in vitro incubations. Rabbit polyclonal antibodies anti rainbow trout leptin were developed and employed to detect leptin concentration in the stomach and in the incubation medium. Stomach slices were incubated with butyric (4:0), oleic (18:1n-9), α-linolenic (18:3n-3) and arachidonic fatty acids (20:4n-6). All fatty acids caused an increase in the protein in both the stomach and culture medium, while leptin transcript was not modified. Overall, the results confirm the gastric leptin release upon nutritional modulation. [ABSTRACT FROM AUTHOR]

NGF, BDNF, NT-3 and their specific receptors TrkA, TrkB and TrkC are known to be involved in the development and maintenance of vertebrates’ nervous system. However, these molecules play a role also in non-neuronal tissue, such as in the reproductive system. In this study we investigated the presence and localization of neurotrophins and Trk receptors to unravel their potential role in the developing and adult ovary of Japanese quail, a model species well suited for reproduction studies. Western blotting analysis on ovaries of three month old quails in the period of egg laying showed the presence of pro and mature forms of neurotrophins and splice variants of Trk receptors. Immunohistochemical investigation reported that in embryonic ovaries from the 9th day of incubation to the hatching NGF and NT-3 were observed in the cortical and medullar areas respectively, whereas Trk receptors were observed in both areas. In adult ovary, all NTs were detected in glandular stromal cells, NGF and NT-3 also in the nervous component. Regarding follicle components, NGF and BDNF were observed in oocytes and follicular cells. All TrK receptors were present in nervous components and only TrkA in glandular stromal cells. In follicles, TrkA was present in oocyte cytoplasm and TrkB in theca cells. The results suggest an involvement of the neurotrophin system in the quail ovary physiology, promoting the oocyte development and follicular organization in the embryo, as well as oocyte and follicular maturation in adults. [ABSTRACT FROM AUTHOR]

Background: The management of patella in total knee arthroplasty (TKA) is a controversial topic. The elevated risk of patellofemoral complications seems to support those who are not eager to perform such procedure. The aim of this study is to carry out a retrospective assessment of patients who underwent patellar resurfacing during TKA from 2004 to 2009.Methods: From January 1, 2004, to December 31, 2009, 1280 patients underwent TKA with patellar prosthesis implantation. Of them, 861 patients (639 women and 222 men with an average age of 67.7 ± 12.3 years) were available for a telephonic interview consisting of the questions included in the Knee Pain Score (KPS). Patients presenting KPS >36 and those subjected to reoperation were then evaluated clinically and radiologically.Results: Of the 861 patients included in the study, 801 showed a KPS <36. Among the remaining 60 patients, 33 had a KPS >36, while 27 had been reoperated at the target knee. In the former case, a decrease in normal Insall-Salvati index and patellar tilt values (from 1.08 to 1.02 and from 9.3 to 9.1, respectively) and average anatomic axis (from -2.6° to 1.1°) was found comparing preoperative with last follow-up evaluation. Only in 0.6% of the cases of reintervention, patellofemoral complications were found to be the cause.Conclusion: This study conducted on a large and homogenous group of patients confirms a very low rate of complications related to patella resurfacing during TKA. [ABSTRACT FROM AUTHOR]

Associations between depression/anxiety and pain are well established, but its directionality is not clear. We examined the associations between temporally previous mental disorders and subsequent self-reported chronic back/neck pain onset, and investigated the variation in the strength of associations according to timing of events during the life course, and according to gender. Data were from population-based household surveys conducted in 19 countries (N = 52,095). Lifetime prevalence and age of onset of 16 mental disorders according to the Diagnostic and Statistical Manual of Mental Disorders, Fourth Edition, and the occurrence and age of onset of back/neck pain were assessed using the Composite International Diagnostic Interview. Survival analyses estimated the associations between first onset of mental disorders and subsequent back/neck pain onset. All mental disorders were positively associated with back/neck pain in bivariate analyses; most (12 of 16) remained so after adjusting for psychiatric comorbidity, with a clear dose-response relationship between number of mental disorders and subsequent pain. Early-onset disorders were stronger predictors of pain; when adjusting for psychiatric comorbidity, this remained the case for depression/dysthymia. No gender differences were observed. In conclusion, individuals with mental disorder, beyond depression and anxiety, are at higher risk of developing subsequent back/neck pain, stressing the importance of early detection of mental disorders, and highlight the need of assessing back/neck pain in mental health clinical settings.Perspective: Previous mental disorders according to the Diagnostic and Statistical Manual of Mental Disorders, Fourth Edition are positively associated with subsequent back/neck pain onset, with a clear dose-response relationship between number of mental disorders and subsequent pain. Earlier-onset mental disorders are stronger predictors of subsequent pain onset, compared with later-onset disorders. [ABSTRACT FROM AUTHOR]

The "zipper model of empathy" has been proposed for psychopathy. It postulates that empathic behavior may fail to arise due to impaired facial emotion recognition. In this study, we examined if the model may be of relevance for schizophrenia. In a sample of participants with schizophrenia and a history of severe interpersonal violence, associations between measures of social cognition (emotion recognition, theory of mind) and aspects of psychopathy (lack of empathy, lack of remorse) were investigated. A non-violent sample experiencing schizophrenia served as a control group. Correlation analyses revealed a specific and statistically significant association between facial emotion recognition and lack of empathy in the violent sample. Follow-up analyses identified that neutral emotions were of particular importance. Logistic regression analyses confirmed that impairments in facial emotion recognition predicted levels of empathy in the violent sample experiencing schizophrenia. Our results suggest that the "zipper model of empathy" may be relevant for schizophrenia. The findings further point to the potential benefit of including social cognitive training in the treatment of persons with schizophrenia and a history of interpersonal aggression. • Impaired facial emotion recognition co-occurs with psychopathy and schizophrenia. • Facial emotion recognition was associated with lack of empathy in schizophrenia. • But only among schizophrenia participants with a history of interpersonal violence. • The results support the validity of the "zipper model of empathy" for schizophrenia. [ABSTRACT FROM AUTHOR]

The expression and localization of leptin (A and B) and its receptor family in control and diet-induced obese (DIO) adult male zebrafish gut, after 5-weeks overfeeding, administering Artemia nauplii, as fat-rich food, were investigated. Recently, the obese adult zebrafish was considered an experimental model with pathophysiological pathways similar to mammalian obesity. Currently, there are no reports about leptin in fish obesity, or in a state of altered energy balance. By qRT-PCR, leptin A and leptin B expression levels were significantly higher in DIO zebrafish gut than in the control group (CTRL), and the lowest levels of leptin receptor mRNA appeared in DIO zebrafish gut. The presence of leptin and its receptor proteins in the intestinal tract was detected by western blot analysis in both control and DIO zebrafish. By single immunohistochemical staining, leptin and leptin receptor immunoreactive endocrine cells were identified in the intestinal tract either in DIO or control zebrafish. Moreover, leptin immunopositive enteric nervous system elements were observed in both groups. By double immunohistochemical staining, leptin and its receptor were colocalized especially in DIO zebrafish. Thus, our study represents a starting point in the investigation of a possible involvement of leptin in control of energy homeostasis in control and DIO zebrafish. [ABSTRACT FROM AUTHOR]

Wave forecasting may represent a useful tool for safety assessment of maritime works and activities. To date, wave forecasting uncertainty is usually corrected by using either the mean calibration factor or the time series method. However, within the frame of maritime work management it is necessary to forecast – with an acceptable probability of error – whether or not the significant wave height at a given location will exceed a prefixed threshold within a specified temporal window, so as to assess the safety of the specified temporal window with respect to the activity to be carried out. The present paper aims to illustrate a general criterion useful to correct wave forecast, i.e. to provide an engineering tool able to assess the safety of the temporal window needed to complete a specified maritime work. The paper provides a detailed description of the method, together with the application to a real case. [ABSTRACT FROM AUTHOR]

This study comprises a critical review of modeling of pesticides in surface waters. The aim was to update the status of the use of models to simulate the fate of pesticides from diffuse sources. ISI papers were selected on Scopus and the information concerning the study areas, type of pesticides (herbicides, fungicides and insecticides), the model, and the methodology adopted (i.e., calibration and/or validation, spatial and temporal scales) were analyzed. The studies were carried out in Europe (55.5%), North America (22.3%), Asia (13.9%) and South America (8.3%). The Soil and Water Assessment Tool proved to be the most used model (45.95%). Herbicides were the most modeled pesticides (71.4%), followed by insecticides (18.2%) and fungicides (10.4%). The main herbicides modeled were atrazine, metolachlor, isoproturon, glyphosate, and acetochlor. Insecticides such as chlorpyrifos and metaldehyde. Chlorothalonil, and fungicides (i.e., tebuconazole) were the most widely investigated. Based on published studies, it was found that modeling approaches for assessing the fate of pesticides are constantly evolving and the model algorithms work well with diverse watershed conditions, management strategies, and pesticide properties. Several papers reported concentrations of pesticides exceeding ecotoxicological thresholds revealing that water contamination with pesticides used in agriculture and urban areas is a priority issue of current global concern. [Display omitted] • A critical review of modeling of pesticides in surface waters is reported. • Modeling approaches for assessing the fate of pesticides are constantly evolving. • SWAT was the one most used on a global scale (35.71%). • Herbicides were the most modeled pesticides followed by insecticides and fungicides. • Concentrations of pesticides exceed ecotoxicological thresholds. [ABSTRACT FROM AUTHOR]

The demand for artificial or bioartificial engineered tissues is increasing today in regenerative medicine techniques to replace and restore the physiological function of damaged tissues. Such engineered constructs hold different properties depending on the tissue to be replicated. As for vascularized tissues, complex biocompatible structures, namely scaffolds, play a key role in supporting oxygen and nutrient supply, thus sustaining tissue neoformation and integration with the host. Scaffold architecture significantly impacts its regenerative potential, while preclinical trials are essential to define scaffold-host interactions. In compliance with the 3 R principle, there is a clear need to optimize both the procedures to evaluate scaffold performance and the analysis methodology decreasing the number of animals required to gain consistent data. In parallel, current technologies used in preclinical research generate huge amounts of data that need to be elaborated and interpreted correctly. Therefore, we designed this study to evaluate the results of scaffold integration with the host tissue after implantation in a mouse subcutaneous pocket model. We evaluated the angiogenic response developed by the host and the degree of scaffold integration by using a combined morphometric approach based on both histological and micro-CT analyses. Six-layer scaffolds, made of polycaprolactone (PCL) microspheres, with an ordered structure were produced by thermal sintering. Scaffolds were then implanted in BALB/c mice and retrieved 21 days post-implantation when the animals were deeply anesthetized and perfused with Microfil, a contrast agent for micro-CT. Here, we describe a method to extract quantitative data from micro-CT reconstructions such as (i) total vessel volume; (ii)% of vessel penetration; (iii) distribution of vessel diameters. The general principle of this approach is the refinement of the region of interest (ROI), thus producing a volume of interest (VOI) that matches scaffold volume. This VOI serves as a dataset from which to extract volumetric information. Then VOIs are divided into three identical parts, proximal, median, and distal, to follow the vessel progression into the scaffold, thus obtaining their depth of penetration (DoP). By this methodology, we observed in mean, among the analyzed samples, a vessel invasion for 1,38 mm3 corresponding to the 1,53% of the scaffold volume. We then looked at the diameter distribution being this value a key indicator of vessel maturity, highlighting that 55% of vessels fall into the range from 5,99–53.99 µm while the remaining 45% are distributed into intervals from 54 to 136 µm. In parallel, to evaluate tissue integration in detail, histological and immunofluorescent analyses were performed to look at vessel distribution and collagen synthesis. Histological results strongly correlate with the micro-CT data providing, however, an overview of the ingrowth tissues. In addition, by immunofluorescent analysis we demonstrate that newly formed vessels are mature at the considered time point and tissue collagen deposition is widespread within the scaffolds. Collectively, we propose a new method to track vessel formation by using a multi-modal approach posing the basis for: i) the fabrication of novel scaffolds for Tissue Engineering; ii) the integration of detailed information for a wide range of morphological and functional analyses. [ABSTRACT FROM AUTHOR]

Neurotrophin-4 (NT-4) is a member of the well-known family of neurotrophins that regulate the development of neuronal networks by participating in neuronal survival and differentiation, the growth of neuronal processes, synaptic development and plasticity, as well as myelination. NT-4 interacts with two distinct receptors: TrkB, high affinity receptor and p75 low-affinity neurotrophin receptor (p75 NTR ). In the present survey, we identified the gene encoding NT-4 in the teleost Nothobranchius furzeri , a model species for aging research. The identified gene shows a similarity of about 72% with medaka, the closest related species. The neuroanatomical localization of NT-4 mRNA is obtained by using an LNA probe. NT-4 mRNA expression is observed in neurons and glial cells of the forebrain and hindbrain, with very low signal found in the midbrain. This survey confirms that NT-4 is expressed in the brain of N. furzeri during adulthood, suggesting that it could also be implicated in the maintenance and regulation of neuronal functions. [ABSTRACT FROM AUTHOR]

Fish constitute the oldest and most diverse class of vertebrates, and are widely used in basic research due to a number of advantages ( e.g. , rapid development ex-utero , large-scale genetic screening of human disease). They represent excellent experimental models for addressing studies on development, morphology, physiology and behavior function in other related species, as well as informative analysis of conservation and diversity. Although less complex, fish share many anatomical and physiological features with mammals, including humans, which make them an important complement to research in mammalian models. In this review we describe and compare the most relevant anatomical features of the most used teleostean species in research, to be taken into consideration when selecting an animal model: zebrafish ( Danio rerio ), medaka ( Oryzias latypes ), the turquoise killifish ( Nothobranchius furzeri ), and goldfish ( Carassius auratus ). Zebrafish and medaka are the mainstream models for genetic manipulability and studies on developmental biology; the turquoise killifish is an excellent model for aging research; goldfish has been largely employed for neuroendocrine studies. [ABSTRACT FROM AUTHOR]

The anatomical features distinctive to each of the very large array of species used in today's biomedical research must be born in mind when considering the correct choice of animal model(s), particularly when translational research is concerned. In this paper we take into consideration and discuss the most important anatomical and histological features of the commonest species of laboratory rodents (rat, mouse, guinea pig, hamster, and gerbil), rabbit, and pig related to their importance for applied research. [ABSTRACT FROM AUTHOR]

Purpose To evaluate the long-term functional results of arthroscopic abrasion arthroplasty for the treatment of full-thickness cartilage lesions of the medial femoral condyle. Methods Between 1990 and 1996, 75 consecutive patients with isolated chondral lesions of the medial femoral condyle were treated with arthroscopic chondral abrasion. A retrospective analysis of the clinical results of this cohort was performed. The patients were evaluated according to the Knee Society Score questionnaire preoperatively, at 10 years postoperatively, and at final long-term follow-up at a mean of 20 years. At final follow-up, they were also assessed according to the Western Ontario and McMaster Universities Osteoarthritis Index. Patients were divided according to the lesion size and by age, and the Kaplan-Meier survivorship function (with second operation taken as an endpoint) for the various groups was calculated. Results At a mean of final follow-up of 20 years (range, 16.94 to 23.94 years), a positive functional outcome (Knee Society Score ≥70 points or no reoperation) was recorded in 67.9% of the patients. Twenty-year survivorship in this cohort was 71.4% (95% confidence interval, 0.5690 to 0.8590). The survivorship was 89.5% for patients younger than 50 years and 55.7% for patients aged 50 years or older. The functional results for patients with lesions smaller than 4 cm 2 were significantly better than those for patients with lesions of 4 cm 2 or greater ( P = .031). There were no statistical differences between patients with and without associated lesions at the time of surgery. Conclusions Our hypothesis that there would be survivorship greater than 86% was disproved. However, arthroscopic abrasion arthroplasty can be a valid treatment for medial femoral condylar full-thickness defects of the knee, even in the long-term, particularly for younger patients and those with smaller lesions. Level of Evidence Level IV, therapeutic case series. [ABSTRACT FROM AUTHOR]

In this work we improved the performance of ITO-free polymer solar cells (PSCs) by incorporating silver nanoparticles (AgNPs) in the highly conductive (HC) PEDOT:PSS anode. The AgNPs were synthetized in-situ in the PEDOT:PSS water dispersion. This anode was used to realize PSCs with the following geometry: glass/HC-PEDOT:PSS/PEDOT:PSS/PBDTTT-C:[70]PCBM/Ca/Al. All the devices were characterized by UV-VIS spectroscopy, impedance spectroscopy, IV light, IV dark and quantum efficiency measurements. The presence of AgNPs in the HC-PEDOT:PSS anode contributes to improve the absorption of the photoactive layer and to lower the resistivity of the anode. [ABSTRACT FROM AUTHOR]

Glial cell-line derived neurotrophic factor (GDNF) and the GFRα co-receptors play a role in the developing enteric nervous system. The co-receptors elicit their action by binding receptor tyrosine kinase RET. This immunohistochemical study reports the presence of GDNF and its specific co-receptor GFRα1 in the cat gastrointestinal apparatus during development, from stage 9 to 22. At stage 9 and 11, immunoreactivity (IR) to GDNF was observed in the cells of mesenchyme of the anterior gut. From stage 14 to 22, GDNF IR was detected in nervous plexuses; moreover, GDNF and GFRα1 IR appeared localized in gastrointestinal endocrine cells. The presence of GDNF in the enteric nervous system and in the endocrine cells suggests an involvement of this neurotrophic factor in the gastrointestinal development. Moreover, the presence of the co-receptor GFRα1 in endocrine cells and its absence in the enteric nervous system seems to indicate a different mode of transduction of GDNF signal. GFRα2 and GFRα3 co-receptors were not detected. [ABSTRACT FROM AUTHOR]

Abstract: BDNF plays an important role in the development and maintenance of visual circuitries in the retina and brain visual centers. In adulthood, BDNF signaling is involved in neural protection and regeneration of retina. In this survey, we investigated the expression of BDNF in the retina of adult Nothobranchius furzeri, a teleost fish employed for age research. After describing the retina of N. furzeri and confirming that the structure is organized in layers as in all vertebrates, we have studied the localization of BDNF mRNA and protein throughout the retinal layers. BDNF mRNA is detectable in all layers, whereas the protein is lacking in the photoreceptors. The occurrence of BDNF provides new insights on its role in the retina, particularly in view of age-related disease of retina. [Copyright &y& Elsevier]

Abstract: Nerve growth factor (NGF) acts on central nervous system neurons, regulating naturally occurring cell death, synaptic connectivity, fiber guidance and dendritic morphology. The dynamically regulated production of NGF beginning in development, extends throughout adult life and aging, exerting numerous roles through a surprising variety of neurons and glial cells. This study analyzes the localization of NGF in the brain of the teleost fish Nothobranchius furzeri, an emerging model for aging research due to its short lifespan. Immunochemical and immunohistochemical experiments were performed by employing an antibody mapping at the N-terminus of the mature chain human origin NGF. Western blot analysis revealed an intense and well defined band of 20kDa, which corresponds to proNGF of N. furzeri. Immunohistochemistry revealed NGF immunoreactivity (IR) diffused throughout all regions of telencephalon, diencephalon, mesencephalon and rhomboencephalon. It was detected in neurons and in glial cells, the latter mostly lining the mesencephalic and rhomboencephalic ventricles. Particularly in neurons, NGF IR was localized in perikarya and, to a less extent, in fibers. The widespread distribution of proNGF suggests that it might modulate numerous physiological functions in the adult brain of N. furzeri. The present survey constitutes a baseline study to enhance the understanding of the mechanisms underlying the role of NGF during aging processes. [Copyright &y& Elsevier]

Abstract: The main difficulty in implementing a Tsunami Early Warning System (TEWS) in the Mediterranean Sea arises from the proximity of the tsunami sources to the coasts at risk. Between few minutes and few tens of minutes are available for a timely warning of a possible approaching tsunami. To date, the only TEWS already operating in the Mediterranean Sea is that run by the Italian Department for Civil Protection at the Island of Stromboli, located north of Sicily in the south of the Tyrrhenian Sea. An active volcano is located on the island. The landslides that often detach from the “Sciara del Fuoco” following eruptive activity may result in the generation of tsunamis that propagate around the island and toward the coasts of Italy. The implemented TEWS is therefore aimed at mitigating the risk of landslide generated tsunamis. The present paper illustrates some of the experimental activities carried out during the last decade aimed at improving the TEWS of Stromboli island. A series of experiments was carried out with the main aim of gaining insight on landslide generated tsunamis. In general, the experimental results were intended to be useful for the definition of forecasting formulae, for the validation of math- ematical models, for the improvement of the knowledge on involved phenomena and for the optimization of detection algorithm. In particular, the physical investigations aimed at improving the TEWS of the Stromboli are detailed. [Copyright &y& Elsevier]

Abstract: The Rio Mannu River Basin (Sardinia, Italy) is undergoing a process of agricultural intensification. Like many Mediterranean areas, this basin is characterized by water shortages and diffuse pollution from agricultural sources. Hence the objective of this study was to develop possible land use and land management scenarios that could constitute an alternative to the current watershed management. Several land use and land management scenarios were formulated and analyzed with local stakeholders, and two were selected and simulated as realistic in consideration of the socio-economical aspects of the study area. Scenario 1 involves agricultural practices that include a reduction in fertilizer use to meet the Water Framework Directive requirements for “good” status of water bodies. Scenario 2 introduces rapeseed cultivation, replacing durum wheat in a small area, to investigate the impact of biofuel plant cultivation on water quality. Each option was assessed by considering the effects on water quality, crop yields and economic benefits. The Soil and Water Assessment Tool (SWAT) model was applied to simulate hydrological processes and evaluate current and future nutrient loads. This model requires adequate streamflow data for calibration and validation. However, as is the case for many Mediterranean basins, insufficient data were available. Therefore, a methodology was developed and tested to calibrate hydrological processes based on the transposition of a parameter set from a gauged catchment located in the same region. This study suggests that a sound use of fertilizers could substantially reduce the amount of nutrients flowing into surface waters, although the effects of such a policy on crop yield and farm income would be negative in some cases. Moreover, the results clearly predict that the replacement of durum wheat with rapeseed (a biofuel crop), could offer a margin of profit, but would have a negative impact on water quality due to increased nutrient losses. Consequently, this option is unsuitable for this area. Furthermore, it can be inferred from these results that the promotion of the use of energy from renewable sources may have a negative impact on the objectives of the EU Water Framework Directive. Clearly, this process needs to be regulated, taking into account environmental and socio-economical aspects. [Copyright &y& Elsevier]

Background: A novel catheter design (HD Mesh ablator, Bard) combining high-density circumferential mapping and direct radiofrequency (RF) energy delivery has been developed to map and isolate the pulmonary veins (PVs). Objective: The purpose of this study was to assess the feasibility of the Mesh catheter for PV isolation in patients with paroxysmal atrial fibrillation (AF). Methods: Twenty consecutive patients (mean age 56.4 ± 12.2 years; 16 men) with paroxysmal drug-refractory AF were referred for ablation. The procedure was performed in a stepwise manner: PV isolation was initially attempted with the Mesh ablator, and if that was not successful, a conventional ablation approach was then used. Results: A total of 73 PVs including seven veins with left common ostium were targeted. Successful deployment of the Mesh was achieved in all but four veins (94.5%). Using the Mesh catheter for ablation, PV isolation was achieved in 46 (63%) of the 73 PVs. The mean (RF) ablation time required to achieve complete isolation was 12.4 ± 6.1 minutes per PV. The Mesh-only approach allowed isolation of all veins in eight (40%) patients. In combination with conventional ablation, successful PV isolation was achieved in 71 (97%) of 73 PVs. No complications attributable to the Mesh ablator occurred in this series. Conclusions: PV isolation using the Mesh catheter is feasible and may simplify the current PV isolation procedures. With the current catheter design, PV isolation could be achieved in 63% of PVs. A larger Mesh diameter with an over-the-wire design may help improve the acute success rate. [Copyright &y& Elsevier]

Abstract: Although there is a growing body of research concerning the prevalence and correlates of chronic pain conditions and their association with mental disorders, cross-national research on age and gender differences is limited. The present study reports the prevalence by age and gender of common chronic pain conditions (headache, back or neck pain, arthritis or joint pain, and other chronic pain) in 10 developed and 7 developing countries and their association with the spectrum of both depressive and anxiety disorders. It draws on data from 18 general adult population surveys using a common survey questionnaire (N = 42,249). Results show that age-standardized prevalence of chronic pain conditions in the previous 12 months was 37.3% in developed countries and 41.1% in developing countries, with back pain and headache being somewhat more common in developing than developed countries. After controlling for comorbid chronic physical diseases, several findings were consistent across developing and developed countries. There was a higher prevalence of chronic pain conditions among females and older persons; and chronic pain was similarly associated with depression-anxiety spectrum disorders in developed and developing countries. However, the large majority of persons reporting chronic pain did not meet criteria for depression or anxiety disorder. We conclude that common pain conditions affect a large percentage of persons in both developed and developing countries. Perspective: Chronic pain conditions are common in both developed and developing countries. Overall, the prevalence of pain is greater among females and among older persons. Although most persons reporting pain do not meet criteria for a depressive or anxiety disorder, depression/anxiety spectrum disorders are associated with pain in both developed and developing countries. [Copyright &y& Elsevier]

Physiological kidney function is closely related to the state of the vascular network. Disorders, such as capillary rarefaction, predispose to chronic kidney disease (CKD). In this context, deepening of the methodologies for studying the renal vascular network can be of basic importance. To meet this need, numerous animal models and, in parallel, several methods have been developed. In this work we propose a protocol to accurately feature kidney vasculature in mouse, however, the same protocol is suitable to be applied also to other animal models. The approach is multiparametric and mainly based on micro-computed tomography (μCT) technique. Micro-ct allows to study in detail the vascular network of any organ by exploiting the possibility to perfuse the sample with a contrast agent. The proposed protocol provides a fast and reliable method to extract quantitative information from the μCT scan by using only the basic functions of the software supplied by the scanner without any additional analysis. Through iterative cropping of the scanned ROI and calculation of a sample-specific threshold we calculated that the average volume of a female BALB/c kidney of eighth weeks is 147.8 mm3 (5.4%). We also pointed out that the average volume of the vascular network is 4.9% (0.3%). In parallel we performed traditional histological and immunofluorescence techniques to integrate the information gained via μCT and to frame them in the tissue context. Vessel count on histological sections showed a different density in the different regions of the organ parenchyma, in detail, vessel density in the cortex was 19.03 ± 2.51 vessels/ROI while in the medulla it was 10.6 ± 1.7 vessels/ROI and 5.4 ± 1.3 vessels/ROI in the outer and inner medulla, respectively. We then studied vessel distribution in the renal parenchyma which showed that the 55% of vascular component is included in the cortex, the 30% in the outer medulla and the 15% in the inner medulla. Collectively, we propose an integrated approach that can be particularly useful in the preclinical setting to characterize the vasculature of any organ accurately and rapidly. [ABSTRACT FROM AUTHOR]

The potential response of flow regimes to future climate has crucial importance for a variety of practical applications, such as sustainable water management and ecological asset preservation. For this study, multi-site investigations of alterations in flow regimes under projected climate change were performed for one of the largest watersheds in Morocco, the Bouregreg Watershed (BW). Future daily streamflow was simulated using the eco-hydrological model Soil and Water Assessment Tool (SWAT) forced by climatic data from CMIP5 model outputs. Simulations were carried out for two periods: 2035–2050 (2040s), and 2085–2100 (2090s) under two emissions scenarios: RCP4.5 and RCP8.5. Future streamflow regimes were examined in accordance with an Indicators of Hydrologic Alteration (IHA) analysis, while being confronted with baseline flow data. Mean temperature has been predicted to increase by up to 2.79 °C in the 2090s (RCP8.5), and a higher variability in rainfall regime is expected. The results indicate that, under future changes in climate patterns, flow regimes in BW will be altered. However, the extent of alteration will be reflected unevenly among the four streams of the study watershed. The IHA analysis revealed that most of the river network will experience both a decrease in monthly flow magnitude (mostly in November and December), and an increase (dominantly in February and March) when compared to baseline. The frequency of flash pulses, and the number of zero-flow days are also expected to increase. Most of the projected alterations were estimated to become very significant in the 2090s under the RCP8.5 scenario. The potential ecological implications were also analysed, and the most sensitive streams of BW were identified to guide local water planning and ecological preservation strategies. • SWAT, CMIP5 data and IHA method used to forecast future flow regimes in Morocco. • Alterations extent will be unevenly distributed over the river network. • Ecological implications are expected due to climate change impact on flow attributes. [ABSTRACT FROM AUTHOR]

• The SWAT model is calibrated and validated in the baseline scenario. • Contour farming reduces soil erosion but is not economically viable. • No tillage shows the most economic advantage in non-steep slope areas. • Reforestation is effective and economically sustainable in steep-slope areas. • Combining contour farming and reforestation is very effective for erosion control. Erosion is the most widespread form of soil degradation in Europe. EU Member States are called to identify areas prone to high risk of soil erosion and to adopt Best Management Practices (BMPs) to decrease land degradation. This study is aimed at identifying effective BMPs and their economic feasibility for controlling soil erosion in south-central Italy where lands are largely cultivated with winter wheat. The Soil and Water Assessment Tool (SWAT) was applied to simulate the baseline hydrologic and soil erosion processes of the Carapelle basin in the Puglia region, Italy. Calibrated sediment loads were reasonably accurate when statistically evaluated against measured data (R2 = 0.5, NSE = 0.5, PBIAS=−2.8 %). The model performed equally well for simulating stream flow rates (R2 = 0.6, NSE = 0.6, PBIAS = 5.3 %). The model maintained reliable performance during the validation period as well. Average annual specific sediment load was estimated 5.95 t ha-1 yr-1 mostly contributed by cultivated croplands. Based on regional agricultural policies, four management scenarios were implemented using the calibrated SWAT model: contour farming (BMP1), no tillage (BMP2); reforestation (BMP3) and contour farming and reforestation (BMP4). A threshold of sediment yield greater than 10 t ha-1yr-1 was selected to discretize target treatment areas where these BMPs were applied. Result show that combining contour farming and reforestation (BMP4) was the most effective (38 % reduction; from 5.95 to 3.70 t ha-1) for erosion control, followed by BMP2 (29 %; from 5.95 to 4.20 t ha-1), BMP1 (22 %; from 5.95 to 4.61 t ha-1) and BMP3 (15 %; from 5.95 to 5.04 t ha-1). An analysis of the farmer return-production cost ratio (FR/PC) indicated that the baseline (conventional tillage) and BMP1 were both economically sustainable in areas with slope <20 % (FR/PC = 1.12 and 1.11, respectively). BMP2 received the highest FR/PC rating of 1.67 in areas with slope <20 %. The baseline scenario had no economic advantage (FR/PC = 0.93) in steep slope areas. BMP3 was ranked at the top (FR/PC = 1.49) followed by BMP2 (FR/PC = 1.41) in areas with slope>20 %. The results show that a program of measures can be effective for controlling soil erosion but it must be implemented over long time frames and it requires relevant investments from the public and private sectors. [ABSTRACT FROM AUTHOR]

Several surgical techniques have been described for the treatment of talar chondral lesions. Among them, microfracture is well established. Autologous matrix-induced chondrogenesis (AMIC), using microfracture and biomaterials, has shown promising results for the treatment of knee osteochondral lesions and has been proposed for the ankle as an open technique. We describe an all-arthroscopic AMIC technique. The benefits of an all-arthroscopic procedure include smaller incisions with less soft-tissue dissection, better visualization of the joint, and a quicker recovery compared with open surgery. The use of matrix to support cartilage regeneration promotes good-quality cartilage tissue with satisfactory long-term outcomes. Our all-arthroscopic AMIC technique uses a type I–type III porcine collagen matrix (Chondro-Gide; Geistlich Pharma, Wolhusen, Switzerland) and is characterized by 2 different arthroscopic surgical phases. First, adequate exposure is achieved through use of a Hintermann spreader (Integra LifeSciences, Plainsboro, NJ) with sufficient joint distraction and wet lesion preparation. The second surgical step is performed dry, involving matrix placement and fixation. The all-arthroscopic AMIC technique for the treatment of osteochondral lesions of the talus allows a very precise reconstruction in the case of cartilage defects and avoids the need for a more invasive operation associated with higher morbidity and a longer surgical time. [ABSTRACT FROM AUTHOR]

This study reports the immunohistochemical localization and distribution of orexin A and B-like and their receptors-like peptides in the gastroenteric tract of chicken. The immunoreactivity is distributed in endocrine cells, nerve fibers and neurons, both in the stomach and intestine, and shows a discrete conformity with the data till now reported for Mammals. Our study suggests a possible participation of orexin-like peptides in the modulation of chicken gastroenteric activities and the preservation of their main distribution compared to Mammals. Western blot analysis has confirmed the presence of prepro-orexin and both receptors in the examined tissues. This survey represents the first evidence of the presence of orexin-like peptides in the gastroenteric tract of non mammalian species, and the results could help to better understand the alimentary control and body weight in domestic birds, which are of relevance to determine the productive factors in breeding animals. This study might also serve as a baseline for future experimental studies on the regulation of the gastroenteric functions in non mammalian Vertebrates. [ABSTRACT FROM AUTHOR]

RET receptor is a transmembrane protein which, together with the glial-cell-line derived neurotrophic factor family receptors alpha, forms a receptor complex upon activation by the glial-cell-line-derived neurotrophic ligands (GFLs). RET signaling is crucial for: (a) development of the enteric nervous system and kidney; (b) development of sympathetic, parasympathetic, motor, and sensory neurons; (c) postnatal maintenance of dopaminergic neurons; (d) spermatogenesis. In humans, RET mutations cause the Hirschsprung's disease, characterized by megacolon aganglionosis, and different types of cancer, the multiple endocrine neoplasia type 2A and type 2B and familial medullary thyroid. In the earliest aged cat embryos studied (stage 9 according to Knopse), RET immunoreactivity (IR) was observed in few cells detected in bilateral rows extending latero-ventrally to the neural tube and dorsolaterally to the foregut. In the successive aged group (stage 11), RET IR was observed in few single or grouped epithelial cells of the anterior gut and in small clustered cells scattered in the mesenchyme around the anterior gut. From stage 14-22 (the last stage 22 includes foetuses around the birth), RET IR was seen in neurons and fibers of the enteric nervous system. The appearance and intensification of RET-IR in the gut occurred with cranio/caudal and external/internal directions during the development. These results, thus, suggest the involvement of GFLs in the neuroblast migration, proliferation and differentiation. For a short period of development, these molecules might also act on some cells of the epithelium. [ABSTRACT FROM AUTHOR]

Abstract: This paper illustrates the results of an experimental investigation (model-to-prototype length ratio equal to 12) carried out to reproduce the cross-shore evolution of nourished sandy beaches. New two-dimensional experiments were performed to study the short-term response of the cross-shore profile for both “soft” (unprotected) and “mixed” (protected by submerged breakwaters) beach fill projects. Due to the simplified reproduction of prototype conditions in a two-dimensional geometry, only cross-shore sediment transport is considered. The results are related to the immediate post-nourishment evolution and far from beach fill boundaries where long-shore gradients of long-shore sediment transport are likely to be negligible. Three different pseudo-random wave trains were generated in order to simulate both accretive and erosive conditions. A fourth wave train, characterised by time-varying incident wave spectrum was generated for the investigation of the beach response to simplified storm time evolution. Dimensionless experimental results are given in terms of wave parameters, key features of cross-shore profile evolution and sediment transport rates. Furthermore, being highly resolved in both time and space, experimental data are suitable for mathematical model validation. It was observed that submerged breakwater switches erosive conditions to slightly accretive, at least within the tested experimental range. [Copyright &y& Elsevier]