13 results on '"Darwish, Hadeer"'
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2. Aphicidal activity of five plant extracts applied singly or in combination with entomopathogenic bacteria, Xenorhabdus budapestensis against rose aphid, Macrosiphum rosae (Hemiptera: Aphididae)
- Author
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Noureldeen, Ahmed, Kumar, Uttam, Asad, Muhammad, Darwish, Hadeer, Alharthi, Sarah, Fawzy, Mustafa A., Al-Barty, Amal M., Alotaibi, Saqer S., Fallatah, Ahmed, Alghamdi, Akram, Albogami, Bander, and Alkashgry, Najla
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- 2022
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3. Assessment of variability among morphological and molecular characters in wild populations of mint [Mentha longifolia (L.) L.] germplasm.
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Devi, Anjina, Iqbal, Tasir, Ahmad Wani, Ishfaq, Sharma, Geeta, Verma, Susheel, Noureldeen, Ahmed, and Darwish, Hadeer
- Abstract
Mentha longifolia is an important medicinal and aromatic perennial herb that exhibits wide distribution range from sub-tropical to temperate regions. In the present study, agro-morphological traits and genetic differences in 19 different populations of M. longifolia were studied to evaluate the level and extent of its diversity. Analysis of variance (ANOVA) showed that the different phenotypic characters show considerable differences among various populations and was significant at p < 0.05. Molecular diversity analysis performed by using arbitrary amplified eleven ISSR primers generated a total of 121 amplicons that range within the size of 200–2500 base pairs (bp). Each primer on average generated 11 amplicons with percentage polymorphism being 100. The analysis of molecular variance (AMOVA) showed more (64%) among population genetic diversity and less (36%) within the populations. Greater genetic differentiation (Gst = 0.6852) among these populations occurs due to low gene flow (Nm = 0.2297) and greater habitat variability. Geographic and genetic distances were positively correlated according to Mantel's test. In order to remove any kind of biases, we used R software to perform cluster and redundancy analysis to analyse the extent of relatedness among studied populations. In terms of morphological and molecular aspects, the populations were grouped into four and five clusters respectively based on hierarchical clustering method. The results demonstrated that M. longifolia displays a great degree of morphological and genetic variation and can be utilized in breeding, genetic improvement, and gene bank conservation programmes in future. [ABSTRACT FROM AUTHOR]
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- 2022
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4. Standardization of in vitro micropropagation of Winter Jasmine (Jasminum nudiflorum) using nodal explants.
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Showkat Bhat, Momin, Ahmad Rather, Zahoor, Tahir Nazki, Imtiyaz, Banday, Neelofar, Wani, Tabinda, Rafiq, Sadaf, Farooq, Iqra, Noureldeen, Ahmed, and Darwish, Hadeer
- Abstract
Present investigation was carried out to arrive at an effective micropropagation protocol for Winter Jasmine (Jasminum nudiflorum) using nodal segments from actively growing plants as explants. Explants were collected from current season shoots during April-May just after the initiation of new flush. Combined sterilization treatment of explants with 1.0% NaOCl 2 for 10 min followed by 70% ethanol for 10 s recorded highest culture survival (63.88%) and optimum culture asepsis (63.88%) followed by the treatment containing 0.1% HgCl 2 for 10 min followed by 70% ethanol for 10 s with culture survival (61.11%) and culture asepsis (69.44%). Highest culture establishment (80.55%) and minimum days to bud sprouting (7.62 days) was recorded with Benzyl adenine + Kinetin (3.0 + 1.0 mgL
−1 ) but maximum length (4.33 cm) and leaf number (7.78) of established micro shoots was recorded with Benzyl adenine + Kinetin (1.0 + 0.5 mgL−1 ). Maximum proliferated shoots (2.41) and an optimum proliferation percentage (77.78 %) was recorded with Benzyl adenine + Kinetin (3.0 + 0.5 mgL−1 ). Minimum size of proliferated shoots (2.02 cm) was recorded with Benzyl adenine + Kinetin (3.0 + 1.0 mgL−1 ) followed by 2.25 cm recorded with Benzyl adenine + Kinetin (3.0 + 0.5 mgL−1 ). Highest rooting (63.93%), primary root number/microshoot (4.74) and longest primary roots (34.67 mm) were recorded with IBA (2.0 mgL−1 ). IBA yielded better results than NAA in terms of higher rooting percentage and root number. However, days to root initiation were found minimum (22.00) with 2.0 mgL−1 of NAA. Highest ex vitro survival of rooted microshoots (89.67%) was recorded with IBA (2.0 mgL−1 ). [ABSTRACT FROM AUTHOR]- Published
- 2022
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5. Application of melatonin and PGPR alleviates thiamethoxam induced toxicity by regulating the TCA cycle in Brassica juncea L.
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Jan, Sadaf, Singh, Bhupender, Bhardwaj, Renu, Kapoor, Dhriti, Kour, Jaspreet, Singh, Rattandeep, Alam, Pravej, Noureldeen, Ahmed, and Darwish, Hadeer
- Abstract
Thiamethoxam, a broad spectrum, neonicotinoid insecticide, is used on various crops including Brassica juncea L. to protect from intruding insects such as leaf-hoppers, aphids, thrips and white-flies. Exposure to thiamethoxam causes acute malady such as tumour development, cell apoptosis, liver damage and neurotoxicity. Melatonin is entailed in umpteen developmental processes of plants, including stress responses. The pleiotropic effects of melatonin in modulating plant growth validate it's imperative contribution as multi-regulatory substance. Exiguous information is known about the role of Pseudomonas putida in improving plant growth under thiamethoxam stress. Taking these aspects into consideration the contemporary study investigates the role of melatonin and Pseudomonas putida strain MTCC 3315 in alleviating the thiamethoxam induced toxicity in B. juncea plant. Fourier Transform Infrared Spectroscopy (FTIR) analysis uncloaked that thiamethoxam induced stress primarily affects the protein content of plant as compared to lipids, carbohydrates and cell wall components. Organic acid profiling of the treated samples carried-out by High-Performance Liquid Chromatography (HPLC), reported an upregulation in the level of organic acids, malic acid (110%), citric acid (170%), succinic acid (81%), fumaric acid (40%) and ascorbic acid (55%) in thiamethoxam treated plants compared to the investigational untreated plants. The melatonin treated seedlings grown under thiamethoxam stress, exhibit increased level of malic acid, citric acid, succinic acid, fumaric acid and ascorbic acid by 81%, 0.94%, 11%, 21% and 6% respectively. Further, thiamethoxam stressed plants inoculated with Pseudomonas putida showed stupendous up-regulation by 161% (malic acid), by 14% (citric acid), by 33% (succinic acid), by 30% (fumaric acid), by 100% (oxalic acid) respectively. Lastly, the combinatorial application of melatonin and Pseudomonas putida resulted in prodigious upsurge of malic acid by 165%, succinic acid by 69%, fumaric acid by 42% respectively in contrast to distinct melatonin and Pseudomonas putida treatments. The accumulation of organic acids ascertains the defence against thiamethoxam stress and corresponds to meet the energy generation requirement to skirmish thiamethoxam mediated abiotic stress in Brassica juncea plant. [ABSTRACT FROM AUTHOR]
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- 2022
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6. Identification, characterization and optimization of phosphate solubilizing rhizobacteria (PSRB) from rice rhizosphere.
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Gupta, Renu, Kumari, Anshu, Sharma, Shiwali, Alzahrani, Othman M., Noureldeen, Ahmed, and Darwish, Hadeer
- Abstract
Two billion people worldwide take rice (Oryza sativa L.) as a staple food. Phosphorus (P) and Nitrogen (N) are the major requirements of rice; although these are available in limited concentrations within rice growing regions. Among different types of Plant growth-promoting rhizobacteria (PGPR), Phosphate solubilizing rhizobacteria (PSRB) constitute an important class. These are known for plant growth promotion by enhancing P and N uptake. PSRB are nowadays used as biofertilizers to restore the soil health. Under the present investigation identification, characterization and optimization of phosphate solubilizing activity of these microbes at different pH, temperature and salt concentrations was carried out. Thirty-seven isolates were recovered from different regions of rice rhizosphere on Pikovskaya (PVK) agar among which 15 isolates were recovered from R.S. Pura, 12 isolates from Bishnah and 10 isolates were recovered from Akhnoor sector of Jammu, India. A prominent halo zone of clearance was developed around the colonies of 12 different isolates, indicating phosphate solubilization activity. Four distinct isolates were amplified, cloned and sequenced for taxonomic identification using 16S primers. The results indicated that PS 1, PS 2, PS 3, PS 4 were related to Pseudomonas aeruginosa, Bacillus subtilis strain 1 , B. subtilis strain 2 , B. subtilis strain 3, respectively. These strains when grown at a wide range of ecological factors showed maximum growth at pH between 6.8 and 8.8, temperature between 28 °C and 37 °C and salinity between 1% and 2%. Screening for phosphate solubilization activity revealed that the halo zone diameter formed by these isolates extended from 2.1 to 3.2 mm. The phosphate solubilizing efficiency (SE) ranged from 35.4 to 50.9 with highest value of 50.9 by PS4 and maximum P solubilization of 10.22 µg/ml was recorded by PS4 at 7th day. Phosphate solubilization activity of these identified PSRB strains can be utilized and explored in the rice growing belts of Jammu region which are deficient in phosphorus. MIC value for zinc sulphate heptahydrate in 12 isolates varied from 1 mg/ml to 6 mg/ml. Phosphate solubilization activity and MIC of these identified PSRB strains can be utilized and explored in the rice growing belts of Jammu region which are deficient in phosphorus. [ABSTRACT FROM AUTHOR]
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- 2022
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7. Phosphate solubilizing bacteria enhanced growth, oil yield, antioxidant properties and biodiesel quality of Kasumbha.
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Nosheen, Asia, Yasmin, Humaira, Naz, Rabia, Keyani, Rumana, Mumtaz, Saqib, Hussain, Syed Babar, Hassan, Muhammad Nadeem, Alzahrani, Othman M., Noureldeen, Ahmed, and Darwish, Hadeer
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Biodiesel is considered as a potential alternative energy source, but problem exists with the quantity and quality of feedstock used for it. To improve the feedstock quality of biodiesel, a field experiment was conducted under natural conditions. Cultivar Thori of kasumbha was used in the experiment. Commercialized biofertilizers were applied at the rate of 20 kg per acre and chemical fertilizer (diammonium phosphate) was applied as half dose (15 kg/ha). Results indicated that number of leaf plant
−1 , leaf area, number of seeds capitulum−1 was significantly increased by biofertilizer treatment alone (BF) and combine treatment of biofertilizer and chemical fertilizer (BFCF). Agronomic traits such as plant height, no. of branches of a plant, no. of capitulum/plant was improved significantly by BF treatment over the control. Maximum 1000 seed weight (41%) and seed yield (23%) were recorded in half dose of chemical fertilizers treatment (CFH). Seed oil content and seed phenolics were significantly improved by BF and CF treatments while maximum biodiesel yield was recorded by BF treatment. Maximum oleic acid was recorded by BF treatment while other fatty acids being maximum in control except linoleic acid in BFCF treatment. Results for specific gravity were non-significant while acid value and free fatty acid contents were substantially reduced by BF treatment as compared to other treatments. Maximum value of iodine number was recorded in BFCF treatment while tocopherol contents were improved by BF treatment. It is inferred that biofertilizer treatment alone perform better as compared to other treatments and 50% chemical fertilizer can be replaced using biofertilizer which is a good approach for sustainable environmental-friendly agriculture. [ABSTRACT FROM AUTHOR]- Published
- 2022
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8. Rhizosphere mediated growth enhancement using phosphate solubilizing rhizobacteria and their tri-calcium phosphate solubilization activity under pot culture assays in Rice (Oryza sativa.).
- Author
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Gupta, Renu, Anshu, Noureldeen, Ahmed, and Darwish, Hadeer
- Abstract
Phosphate solubilizing rhizobacteria are considered as an important alternative to increase the availability of accumulated phosphates through solubilization. These increase the growth of plant by enhancing the efficiency of fixing biological nitrogen. This was studied through a pot experiment involving two Phosphate Solubilizing Rhizobacteria (PSRB) isolates, Pseudomonas aeruginosa and Bacillus subtilis along with Tri-calcium phosphate (TCP) on availibity of nutrients, biological composition of soil and yield attributes of rice crop at its growth stages. Experiment was laid in factorial completely randomized design (CRD) comprising of eight treatments replicated thrice with two factors viz. factor 1 with or without TCP (1 g
−1 soil) and factor 2 with single or combined inoculation of PSRB isolates. Considerable enhancement in available content of potassium (K), phosphorous (P), nitrogen (N) in soil was found with TCP 1 g−1 soil (P 1) and consortium of Pseudomonas aeruginosa and Bacillus subtilis broth culture at crop growth stages. Highest increase in available N (17.13% and 19.1%), available P (232% and 265%), available K (19.6% and 29.2%) over control were recorded in B 3 (consortium of Pseudomonas aeruginosa and Bacillus subtilis broth culture). Similarly, maximum nutrient uptake N (6.4%), P (15.8%) and K (8.9%) were recorded with same treatment. A considerable growth in soil microbial biomass carbon and dehydrogenase activity at crop growth stages was recorded on application of TCP 1 g−1 soil (P 1) and consortium of PSRB isolates' Pseudomonas aeruginosa and Bacillus subtilis (B 3). Highest increase in microbial biomass carbon (16.4% and 16.5%) and dehydrogenase activity 34.7% and 43.8% over control were recorded in B 3 (consortium of PSRB isolates Pseudomonas aeruginosa and Bacillus subtilis) and was found best among all treatments in terms of yield (63.2%) and yield attributes; number of panicles−1 plant (54.8%), number of grains−1 panicle (156%) and average panicle length (63.9%). [ABSTRACT FROM AUTHOR]- Published
- 2021
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9. Potential significance of medicinal plants in forensic analysis: A review.
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Alotaibi, Saqer S., Alshoaibi, Doaa, Alamari, Hala, Albogami, Sarah, Khan, Eman, Alshanbari, Areej, Darwish, Hadeer, Alshanqiti, Bashaer, Alghamdi, Hanan, and Almalki, Wafa
- Abstract
Medicinal plants are a two-edged sword that might be exploited as a treatment specific dosage, and as deadly poisonous substances to commit murder or suicide when administered in high doses. Forensic experts can collect traces and residual materials from these toxic medicinal plants at a crime scene as forensic evidence. Further, more investigations need to be deeply implemented to in the future to understand the significance of medicinal plants in forensic investigations to detect these criminal offenses. Additionally, to provides a deep understanding of chemical substances that can impact human life positively or negatively with different doses as well as identifying the optimal or overdose concentrations for either treatments or poisonous effects using recent biotechnological approaches. This review aims to illustrate different contributions and the significance of medicinal plants in the field and further employment in the context of forensic science, especially in the Kingdom of Saudi Arabia. [ABSTRACT FROM AUTHOR]
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- 2021
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10. Degradation of chlorpyriphos and polyethylene by endosymbiotic bacteria from citrus mealybug.
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Ibrahim, Shahida, Gupta, Rakesh Kumar, War, Abdul Rasheed, Hussain, Barkat, Kumar, Amit, Sofi, Tariq, Noureldeen, Ahmad, and Darwish, Hadeer
- Abstract
Chlorpyriphos is one of the major organophosphorus pesticides used widely to control a range of insect pests across several crops. This insecticide is hazardous to the environment and toxic to mammals, thus, it is essential to remove the same from the environment. Similarly, use of polythene is also increasing day by day. Therefore, it is highly important to identify ways to degrade chlorpyriphos and other pesticides from the environment. We studied the degradation of chlorpyriphos and polyethylene by Citrus mealybug (Planococcus citri) bacterial endosymbionts such as Bacillus licheniformis, Pseudomonas cereus, Pseudomonas putida and Bacillus subtilis. This investigation revealed that bacterial endosymbionts use the polythene as a source of carbon and solubilize them by their enzymatic machinery. The degradation of polyethylene by endosymbionts showed a significant reduction in weight of polyethylene sheet after 15, 30 and 45 days of treatment. The SEM images showed localized degradation of the polyethylene around the bacterial cells in the biofilm. Further, the tensile strength (percentage elongation) was significantly reduced after 45 days of incubation. The weight of paraffin wax showed significant reduction in B. cereus. A significant reduction in total amount of chlorpyriphos in soil was observed at an interval of 7, 14 and 21 days after treatment by the bacterial isolates. Among the bacteria, B. cereus and P. putida were found to be most effective. The results from this study show that endosymbionts can be significantly implicated in degrading chlorpyriphos and polyethylene from the environment. [ABSTRACT FROM AUTHOR]
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- 2021
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11. Preparation and quality by design assisted (Qb-d) optimization of bioceramic loaded microspheres for periodontal delivery of doxycycline hyclate.
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Jain, Pooja, Garg, Abhinav, Farooq, Uzma, Panda, Amulya K., Mirza, Mohd. Aamir, Noureldeen, Ahmed, Darwish, Hadeer, and Iqbal, Zeenat
- Abstract
PLGA (Lactic- co-glycolic acid) coated chitosan microspheres loaded with hydroxyapatite and doxycycline hyclate complex were developed in the present study for periodontal delivery. A modified single emulsion method was adopted for the development of microspheres. Formulation was optimized on the basis of particle size, drug loading and encapsulation efficiency with the central composite design using 2
3 factorial design. Microspheres were optimized and electron microscopy revealed their spherical shape and porous nature. In-vitro study showed initial burst and then sustained release behavior of the formulation for 14 days. Further, in-vitro antibacterial study performed on E. coli (ATCC-25922) and S. aureus (ATCC-29213) revealed concentration dependent activity. Also, in-vitro cyto-toxicity assessment ensures biocompatibility of the formulation with the fibroblast's cells. Overall, the quality by design assisted PLGA microspheres, demonstrated the desired attributes and were found suitable for periodontal drug delivery. [ABSTRACT FROM AUTHOR]- Published
- 2021
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12. Enhancing remediation of RDX-contaminated soil by introducing microbial formulation technology coupled with biostimulation.
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Khan, Mohd Aamir, Sharma, Abhishek, Yadav, Sonal, Celin, S. Mary, Sharma, Satyawati, Noureldeen, Ahmed, and Darwish, Hadeer
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SOIL remediation ,LIQUID chromatography-mass spectrometry ,HIGH performance liquid chromatography ,WHEAT straw ,SOIL degradation - Abstract
The degradation of Hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) through bioaugmentation and biostimulation was investigated. Aqueous phase experiment established the potential of Kinneretia asachharophila strain 12853 in invigorating the RDX-degradation. The microbe denitrified 75.7% RDX from the minimal salt medium by utilizing it as a nitrogen source based on nitrite production. High-Performance Liquid Chromatography (HPLC) and Liquid Chromatography-Mass Spectrometry (LCMS) analyses confirmed the presence of methylenedinitramine (MEDINA) and 4-nitro-2,4-diazabutanal (NDAB) as intermediate metabolites. The microbe formulated into powder and beads showed 29% and 14% loss in viability, respectively, after a storage period of 3 months at 25 °C. The third type of formulation, i.e., water-dispersible granules (WDG), retained 98.52% viability at similar experimental conditions. The microcosm studies revealed a 40% RDX degradation in soil using WDG, while unformulated fresh cultures showed 29% degradation. The process became 1.9–2.4 times more efficient when WDG coupled with stimulants, wheat straw, or sucrose (0.5% w/w). We recorded first-order degradation kinetics with an RDX soil half-life of 16.54–24.15 days. The current study demonstrates the enhanced RDX remediation from the contaminated soil using a robust microbial formulation compatible with wheat straw as an organic stimulant. [Display omitted] • Kinneretia asachharophila aerobically utilized RDX as a nitrogen source in the aqueous phase. • Methylenedinitramine and 4-nitro-2,4-diazabutanal were detected as RDX intermediate metabolites. • K. asachharophila formulated into beads, powder, and water-dispersible granules (WDG). • 1.9 times enhanced RDX degradation achieved through combined use of WDGs and wheat-straw in soil. [ABSTRACT FROM AUTHOR]
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- 2021
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13. Multi-biomarker approach in Mytilus galloprovincialis and Ruditapes decussatus as a predictor of pelago-benthic responses after exposure to Benzo[a]Pyrene.
- Author
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Dellali, Mohamed, Hedfi, Amor, Ali, Manel Ben, Noureldeen, Ahmed, Darwish, Hadeer, Beyrem, Hamouda, Gyedu-Ababio, Thomas, Dervishi, Aida, Karachle, Paraskevi K., and Boufahja, Fehmi
- Subjects
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MYTILUS galloprovincialis , *PYRENE , *SUPEROXIDE dismutase , *DOMOIC acid , *ACETYLCHOLINESTERASE , *GLUTATHIONE , *BIOMARKERS , *CLAMS - Abstract
This study evaluated the biomarker responses indicative of exposure to Benzo[a] Pyrene (B[ a ]P) in Mytilus galloprovincialis and Ruditapes decussatus. A significant increase of the total oxyradical scavenging capacity (TOSC) was observed after seven days of exposure to two concentrations of B[ a ]P (100 and 300 μg.L−1), in the digestive gland with the lowest concentration tested. The TOSC in the gills increased notably only after the exposure to 300 μg.L−1 of B[ a ]P. Interestingly, the superoxide dismutase (SOD) and catalase (CAT) activities in gills and digestive gland on one hand and glutathione S-transferase (GST) in gills in the other, were positively correlated with the concentration of B[ a ]P with a significant induction noticed at the highest concentration. In contrast, a significant increase of the GST activity was observed in the digestive gland following the exposure of bivalves to 100 μg.L−1. In pelagic (M. galloprovincialis) or benthic (R. decussatus) bivalves, the AChE activity decreased discernibly in digestive glands and gills with the increase of B[ a ]P concentrations as evidence of neurotoxic effects. In clams, the exposure to B[ a ]P was followed by a significant increase of Malondialdehyde level (MDA) in gills and digestive gland, this does not occur in gills of Mytilus galloprovincialis at the concentration of 100 μg.L−1. Overall, the results found seems to indicate that the mussel was more suitable as a predictor tool of toxicity of B[ a ]P. [Display omitted] • B[a]P induces phases I and II enzymes in Mytilus galloprovincialis and Ruditapes decussatus. • An inhibition of AChE activity was observed in R. decussatus (gills and digestive gland). • M. galloprovincialis biomarker responses to B[ a ]P were mostly higher than those of R. decussatus. [ABSTRACT FROM AUTHOR]
- Published
- 2021
- Full Text
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