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• A guiding mechanism is designed to enhance the stiffness along the DOC. • The nonlinearity of the guiding mechanism is modeled for large-stroke applications. • The model improves the kinetostatic accuracy of a beam with fillets effectively. • Simultaneous optimal design of topology and size is proposed for the mechanism. • Experiments are carried out to illustrate the efficiency of the proposed mechanism. The large stroke is usually realized by lowering stiffness along the degrees of freedom, which usually leads to a decrease of stiffness along the degrees of constraint. A high stiffness ratio is crucial for reducing the deflections induced by disturbances such as eccentric force and the gravity of the load. Besides, the fatigue life of these mechanisms will be shortened due to the large stress caused by the large deflection. This paper proposed a new guiding mechanism using orthogonally oriented flexures to improve the stiffness along the degrees of constraint and reduce stress concentration. The energy method is utilized to obtain the kinetostatic model of the guiding mechanism by taking the nonlinear deflection into account, based on which the mechanism is optimized by simultaneously considering the topology and size. As compared to the performance of traditional design obtained through the same optimization objective and constraints, the optimal design effectively improves the stiffness along the degrees of constraint, whose accuracy is also validated by the finite element analysis results. Experimental results show that the proposed mechanism increases the stiffness ratio K a z / K x by 3.54 times compared to the traditional mechanism. [ABSTRACT FROM AUTHOR]

• A cellulolytic Acetobacter orientalis XJC-C was identified from a soft coral. • Strain XJC-C has a strong resistance to high salt and temperature. • Strain XJC-C enhances the composting efficiency of banana residues. • Strain XJC-C improves functional diversity of microbial community during composting. • Strain YS-26 enhances metabolism capacity of carboxylic acids and carbohydrates. Banana residues are an important energy resource after fruit harvesting. The optionally dumping and burning causes severely environmental problems. Traditional compost efficiency was limited by lignocellulosic composition of banana residues. Inoculation with cellulase-producing microbes provides an efficient strategy for improving degradation of lignocellulosic materials. In our study, a newly isolated cellulolytic bacterium Acetobacter orientalis XJC-C with a salt and high temperature resistance was identified from a marine soft coral. By contrast, the strain can biodegrade different lignocellulosic agricultural residues, especially banana straw. The highest cellulolytic and ligninolytic enzyme activities were detected during composting at 40 days. Compared with the negative and positive control groups, the lignin degradation rate reached 76.24% in the A. orientalis XJC-C group, increased by 47.08% and 21.85%, respectively. Moreover, the strain improved significantly the metabolic activity and functional diversity of bacterial community. Hence, A. orientalis XJC-C will be a promising candidate for degrading lignocellulosic agricultural residues. [ABSTRACT FROM AUTHOR]