Mujović, Milo, Šojić, Branislav, Danilović, Bojana, Kocić-Tanackov, Sunčica, Ikonić, Predrag, Đurović, Saša, Milošević, Sanja, Bulut, Sandra, Đorđević, Natalija, Savanović, Jovo, and Pavlić, Branimir
Fennel (Foeniculum vul gare) essential oil (EO) and its supercritical fluid extracts (SFE1 and SFE2), as well trans -anethole as a standard compound, were added in basic formulation of beef burgers, at two levels (0.075 and 0.150 μL/g). The main goal of this study was to evaluate the antioxidative and antimicrobial effects of fennel extracts (EO and SFEs) in beef burgers during three days of refrigerated storage. The preservative potential for controlling L. monocytogenes and E. coli growth in these meat products was also assessed. Both types of fennel extracts (EO and SFEs) significantly (P < 0.05) reduced lipid and protein oxidation and microbiological growth (aerobic mesophilic bacteria, lactic acid bacteria, and Enterobacteriaceae) in beef burgers. Results indicated that SFE1 and SFE2 had a higher antioxidative potential than EO and trans -anethole. Higher concentration (0.150 μL/g) of fennel extracts (EO and SFEs) and trans -anethole efficiently inhibited the growth of L. monocytogenes. Therefore, the results of this study displayed significant antioxidative and antimicrobial potential of fennel EO and, especially, SFEs as novel natural ingredients in beef burger processing. • Fennel essential oil (EO) and extracts (SFE1 and SFE2) were added in beef burgers. • Prevented oxidative reactions and inhibition of microbial growth were observed. • SFE extracts were more potent antioxidants and antimicrobials comparing to EO. • Fennel EO and SFEs could be used as novel natural additives in meat processing. [ABSTRACT FROM AUTHOR]