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Methods to assess the performance of a building have been developed for decades, however, many buildings still do not satisfy their occupants in their indoor comfort preferences. This paper presents methods to generate insights from semantic digital twins on the perceived comfort levels of individuals to tighten the as-designed and as-perceived building performance gap. This paper first reviews existing personal indoor comfort models and shares state-of-the-art semantic web technologies in this domain. The paper then presents a generic framework to integrate heterogeneous data into knowledge graphs and use them in data mining processes. This framework is then applied to a case study in the Vertigo building in Eindhoven, The Netherlands. A wide range of information is collected, including building information models, indoor and outdoor sensor data, personal information, and feedback on indoor environmental quality. The integrated data are then used to create personal comfort models. First, multinomial logistic regression models are used to predict future dissatisfaction, after which a latent class analysis created cohorts of people with similar indoor comfort preferences. The results are stored back into the knowledge graph, after which they could be used in other applications, such as to perform occupant-centric control of systems. The methods presented in this paper are summarized in a generic framework that can be used and extended to other domains that aim to combine data integration and data mining. [ABSTRACT FROM AUTHOR]

Protective antibodies against HIV-1 require unusually high levels of somatic mutations introduced in germinal centers (GCs). To achieve this, a sequential vaccination approach was proposed. Using HIV-1 antibody knockin mice with fate-mapping genes, we examined if antigen affinity affects the outcome of B cell recall responses. Compared to a high-affinity boost, a low-affinity boost resulted in decreased numbers of memory-derived B cells in secondary GCs but with higher average levels of somatic mutations, indicating an affinity threshold for memory B cells to enter GCs. Furthermore, upon boosting local lymph nodes (LNs), the composition of primary GCs was modified in an antigen-affinity-dependent manner to constitute less somatically mutated B cells. Our results demonstrate that antigen affinity and location of the boost affect the outcome of the B cell recall response. These results can help guide the design of vaccine immunogens aiming to selectively engage specific B cell clones for further diversification. [Display omitted] • A higher-affinity boost recruits more memory B cells to secondary GCs • A lower-affinity boost recruits memory B cells with more mutations to secondary GCs • GC refueling increases numbers of originally activated B cells with lower levels of mutations Recall responses form the basis of vaccine-induced protection. Termote et al. use genetically modified mice to study the influence of affinity on B cells during boosting. The results indicate an affinity-based threshold for memory B cell entry to germinal centers (GCs). GC refueling promotes the maturation of naive and less mutated B cells. [ABSTRACT FROM AUTHOR]

Most car manufacturers predict that in the first half of the next decade there will be fully autonomous vehicles on our roads. Such vehicles would have to communicate in order to mitigate problems caused by single-viewpoint approach. So there are a lot of researches and developments when it comes to communication layer of V2X (Vehicle-to-Everything), but there is still a lot to be done when it comes to data layer of this communication. This is why we propose using Semantic Web Technologies (SWT) to fill in gaps within data layer of V2X communication. By using SWT (Semantic Web Technologies) and Linked data, we plan to interconnect various data sources, in order to provide homogeneous way for connected autonomous vehicles (CAV) to access relevant information. Such information is currently contained in three distinctive type of sources. These are: Geo-stationary Static data sources (Maps, City models), Geostationary Dynamic data sources (IoT devices) and Non-geostationary Dynamic sources (Vehicles). Using SWT, our goal is to develop ontology(s), in such a way that in-vehicle algorithms can extract and process information about environment they are in, while taking into account available network bandwidth. [ABSTRACT FROM AUTHOR]

A novel theoretical model that describes how aggression among unacquainted pigs is a function of group size is presented. The conclusion states that pigs are able to alter their behavior according to how the actual competitive situation changes with group size.

Toxicological risk assessment of contaminants of emerging concern (CEC) in (sources of) drinking water is required to identify potential health risks and prioritize chemicals for abatement or monitoring. In such assessments, concentrations of chemicals in drinking water or sources are compared to either (i) health-based (statutory) drinking water guideline values, (ii) provisional guideline values based on recent toxicity data in absence of drinking water guidelines, or (iii) generic drinking water target values in absence of toxicity data. Here, we performed a toxicological risk assessment for 163 CEC that were selected as relevant for drinking water. This relevance was based on their presence in drinking water and/or groundwater and surface water sources in downstream parts of the Rhine and Meuse, in combination with concentration levels and physicochemical properties. Statutory and provisional drinking water guideline values could be derived from publically available toxicological information for 142 of the CEC. Based on measured concentrations it was concluded that the majority of substances do not occur in concentrations which individually pose an appreciable human health risk. A health concern could however not be excluded for vinylchloride, trichloroethene, bromodichloromethane, aniline, phenol, 2-chlorobenzenamine, mevinphos, 1,4-dioxane, and nitrolotriacetic acid. For part of the selected substances, toxicological risk assessment for drinking water could not be performed since either toxicity data (hazard) or drinking water concentrations (exposure) were lacking. In absence of toxicity data, the Threshold of Toxicological Concern (TTC) approach can be applied for screening level risk assessment. The toxicological information on the selected substances was used to evaluate whether drinking water target values based on existing TTC levels are sufficiently protective for drinking water relevant CEC. Generic drinking water target levels of 37 μg/L for Cramer class I substances and 4 μg/L for Cramer class III substances in drinking water were derived based on these CEC. These levels are in line with previously reported generic drinking water target levels based on original TTC values and are shown to be protective for health effects of the majority of contaminants of emerging concern evaluated in the present study. Since the human health impact of many chemicals appearing in the water cycle has been studied insufficiently, generic drinking water target levels are useful for early warning and prioritization of CEC with unknown toxicity in drinking water and its sources for future monitoring. [ABSTRACT FROM AUTHOR]

Exposure to Perfluoroalkyl acids (PFAS) can impair human reproductive function, e.g., by delaying or advancing puberty, although their mechanisms of action are not fully understood. We therefore set out to evaluate the relationship between serum PFAS levels, both individually and as a mixture, on the Hypothalamic-Pituitary-Gonadal (HPG) axis by analyzing serum levels of reproductive hormones and also kisspeptin in European teenagers participating in three of the HBM4EU Aligned Studies. For this purpose, PFAS compounds were measured in 733 teenagers from Belgium (FLEHS IV study), Slovakia (PCB cohort follow-up), and Spain (BEA study) by high performance liquid chromatography-tandem mass spectrometry (HPLC/MS) in laboratories under the HBM4EU quality assurance quality control (QA/QC) program. In the same serum samples, kisspeptin 54 (kiss-54) protein, follicle-stimulating hormone (FSH), total testosterone (TT), estradiol (E2), and sex hormone-binding globulin (SHBG) levels were also measured using immunosorbent assays. Sex-stratified single pollutant linear regression models for separate studies, mixed single pollutant models accounting for random effects for pooled studies, and g-computation and Bayesian kernel machine regression (BKMR) models for the mixture of the three most available (PFNA, PFOA, and PFOS) were fit. PFAS associations with reproductive markers differed according to sex. Each natural log-unit increase of PFOA, PFNA, and PFOS were associated with higher TT [18.41 (6.18; 32.31), 15.60 (7.25; 24.61), 14.68 (6.18; 24.61), respectively] in girls, in the pooled analysis (all studies together). In males, G-computation showed that PFAS mixture was associated with lower FSH levels [-10.51 (−18.81;-1.36)]. The BKMR showed the same patterns observed in G-computation, including a significant increase on male Kiss-54 and SHBG levels. Overall, effect biomarkers may enhance the current epidemiological knowledge regarding the adverse effect of PFAS in human HPG axis, although further research is warranted. [Display omitted] • The relationship of PFAS, reproductive hormones and kiss-54 levels was investigated. • Some PFAS were associated with higher TT levels in females. • The PFAS mixture (PFOA, PFOS, PFNA) showed the same trends in female. • PFAS were associated with lower hormones and higher SHBG in males. • PFAS mixture was also associated with higher kiss-54 in males. [ABSTRACT FROM AUTHOR]

The European Joint Programme HBM4EU coordinated and advanced human biomonitoring (HBM) in Europe in order to provide science-based evidence for chemical policy development and improve chemical management. Arsenic (As) was selected as a priority substance under the HBM4EU initiative for which open, policy relevant questions like the status of exposure had to be answered. Internal exposure to inorganic arsenic (iAs), measured as Toxic Relevant Arsenic (TRA) (the sum of As(III), As(V), MMA, DMA) in urine samples of teenagers differed among the sampling sites (BEA (Spain) > Riksmaten adolescents (Sweden), ESTEBAN (France) > FLEHS IV (Belgium), SLO CRP (Slovenia)) with geometric means between 3.84 and 8.47 μg/L. The ratio TRA to TRA + arsenobetaine or the ratio TRA to total arsenic varied between 0.22 and 0.49. Main exposure determinants for TRA were the consumption of rice and seafood. When all studies were combined, Pearson correlation analysis showed significant associations between all considered As species. Higher concentrations of DMA, quantitatively a major constituent of TRA, were found with increasing arsenobetaine concentrations, a marker for organic As intake, e.g. through seafood, indicating that other sources of DMA than metabolism of inorganic As exist, e.g. direct intake of DMA or via the intake of arsenosugars or -lipids. Given the lower toxicity of DMA(V) versus iAs, estimating the amount of DMA not originating from iAs, or normalizing TRA for arsenobetaine intake could be useful for estimating iAs exposure and risk. Comparing urinary TRA concentrations with formerly derived biomonitoring equivalent (BE) for non-carcinogenic effects (6.4 μg/L) clearly shows that all 95th percentile exposure values in the different studies exceeded this BE. This together with the fact that cancer risk may not be excluded even at lower iAs levels, suggests a possible health concern for the general population of Europe. [ABSTRACT FROM AUTHOR]

Colloidal phosphorus (P) may represent an important fraction of the P in natural waters, but these colloids remain poorly characterized. In this work, we demonstrate the applicability of asymmetric flow field-flow fractionation (AF4) coupled to high resolution ICP-MS for the characterization of low concentrations of P-bearing colloids. Colloids from five streams draining catchments with contrasting properties were characterized by AF4-ICP-MS and by membrane filtration. All streams contain free humic substances (2–3 nm) and Fe-bearing colloids (3–1200 nm). Two soft water streams contain primary Fe oxyhydroxide-humic nanoparticles (3–6 nm) and aggregates thereof (up to 150 nm). In contrast, three harder water streams contain larger aggregates (40–1200 nm) which consist of diverse associations between Fe oxyhydroxides, humic substances, clay minerals, and possibly ferric phosphate minerals. Despite the diversity of colloids encountered in these contrasting streams, P is in most of the samples predominantly associated with Fe-bearing colloids (mostly Fe oxyhydroxides) at molar P:Fe ratios between 0.02 and 1.5. The molar P:Fe ratio of the waters explains the partitioning of P between colloids and truly dissolved species. Waters with a high P:Fe ratio predominantly contain truly dissolved species because the Fe-rich colloids are saturated with P, whereas waters with a low P:Fe ratio mostly contain colloidal P species. Overall, AF4-ICP-MS is a suitable technique to characterize the diverse P-binding colloids in natural waters. Such colloids may increase the mobility or decrease the bioavailability of P, and they therefore need to be considered when addressing the transport and environmental effects of P in catchments. [ABSTRACT FROM AUTHOR]

Phosphorus (P) in natural waters may be bound to iron (Fe) bearing colloids. However, the natural variation in composition and P binding strength of these colloids remain unclear. We related the composition of “coarse colloids” (colloids in the 0.1–1.2 μm size range) in 47 Belgian streams to the chemical properties of the streamwater. On average, 29% of the P in filtered (<1.2 μm) samples of these streams is present in coarse colloids. The concentration of Fe-rich colloids in streams decreases with increasing water hardness and pH. The P bearing colloids in these streams mostly consist of Fe hydroxyphosphates and of Fe oxyhydroxides with surface adsorbed P, which is underpinned by geochemical speciation calculations. In waters with molar P:Fe ratios above 0.5, only a minor part of the P is bound to coarse colloids. In such waters, the colloids have molar P:Fe ratios between 0.2 and 1 and are, therefore, nearly saturated with P. Conversely, in streams with molar P:Fe ratios below 0.1, most of the P is bound to Fe-rich colloids. Equilibration of synthetic and natural Fe and P bearing colloids with a zero sink reveals that colloids with low molar P:Fe ratios contain mostly nonlabile P, whereas P-saturated colloids contain mostly labile P which can be released within 7 days. Equilibration at a fixed free orthophosphate activity shows that the Fe-rich colloids may bind only limited P through surface adsorption, in the range of 0.02–0.04 mol P (mol Fe) −1 . The P:Fe ratios measured in naturally occurring Fe and P bearing colloids is clearly higher (between 0.05 and 1). These colloids are therefore likely formed by coprecipitation of P during oxidation of Fe(II), which leads to the formation of Fe hydroxyphosphate minerals. [ABSTRACT FROM AUTHOR]

Riparian wetlands are proposed to mitigate diffuse pollution of surface water by pesticides in agricultural landscapes. Wetland ecosystems though are highly dynamic environments and seasonal disturbances such as freezing and drying can affect microbial population sizes in the sediment and their functionality including pesticide biodegradation, which has hardly been studied. This study examined the effect of artificially induced dry-wet or freeze-thaw events on the mineralization of the pesticides isoproturon (IPU) and 2-methoxy-4-chlorophenoxy acetic acid (MCPA) in wetland microcosms, either without or with prior enrichment of IPU/MCPA degrading populations. Without prior enrichment, mineralization of IPU and MCPA was significantly reduced after exposure to especially freeze-thaw events, as evidenced by lower mineralization rates and longer lag times compared to non-exposed microcosms. However, herbicide mineralization kinetics correlated poorly with cell numbers of herbicide mineralizers as estimated by a most probable number (MPN) approach and the number of IPU and MCPA mineralizers was unexpectedly higher in freeze-thaw and dry-wet cycle exposed setups compared to the control setups. This suggested that the observed effects of season-bound disturbances were due to other mechanisms than decay of pesticide mineralizers. In addition, in systems in which the growth of pesticide mineralizing bacteria was stimulated by amendment of IPU and MCPA, exposure to a freeze-thaw or dry-wet event only marginally affected the herbicide mineralization kinetics. Our results show that season bound environmental disturbances can affect pesticide mineralization kinetics in wetlands but that this effect can depend on the history of pesticide applications. [ABSTRACT FROM AUTHOR]

Vocal attack time (VAT) is the time lag between the growth of sound pressure (SP) and electroglottographic (EGG) signals at vocal initiation. The characteristics of voice initiation are associated with issues of vocal hygiene, efficiency, and quality. Vocal onsets have commonly been qualitatively characterized into three types: hard, simultaneous, and breathy. This study examines the effect of voice onset type on VAT values in normal speakers. SP and EGG recordings were obtained for 55 female and 57 male subjects while producing multiple tokens of three tasks (sustained /α/ and "always" as unaspirated onsets, and "hallways" as an aspirated onset). Results revealed a significant effect of onset type on VAT, with the mean VAT for the "hallways" (aspirated) task greater than the mean VAT for the sustained /α/ and "always" (unaspirated) tasks. There was no significant VAT difference between the sustained /α/ and "always" tasks. Findings confirm the sensitivity of the VAT measure to vocal onset type and suggest its potential application as an objective and quantitative clinical measure of the type of vocal onset. [ABSTRACT FROM AUTHOR]

Summary The purpose of this study was to investigate how vocal attack time (VAT) varies when young adults articulate the three vertex vowels in Mandarin Chinese at five linguistically unconstrained pitch levels. Sound pressure and electroglottographic signals were recorded simultaneously from 53 male and 53 female subjects saying sustained /A/, /i/, and /u/ at five equally spaced pitch heights, each being higher than the preceding one. Then analyses of means, variance, and correlation were performed to explore the relationships of VAT/pitch levels and VAT/vowels. Findings were As mean STs (semitone) increase linearly from levels 1 to 5, mean VATs decrease nonlinearly in a big group of subjects but increase nonlinearly in a small group of them. Based on the body-cover model of F 0 control, data here lead to the guess that different people incline to use different strategies in increasing pitch height. When males, females, and males plus females are considered as a whole, average STs and VATs tend to be positively correlated among the three vertex vowels. [ABSTRACT FROM AUTHOR]

Redox reactions involving iron (Fe) strongly affect the mobility of phosphorus (P) and its migration from agricultural land to freshwater. We studied the transfer of P from groundwater to open drainage ditches in an area where, due to Fe(II) rich groundwater, the sediments of these ditches contain accumulated Fe oxyhydroxides. The average P concentrations in the groundwater feeding two out of three studied drainage ditches exceeded environmental limits for freshwaters by factors 11 and 16, but after passing through the Fe-rich sediments, the P concentrations in the ditch water were below these limits. In order to identify the processes which govern Fe and P mobility in these systems, we used diffusive equilibration in thin films (DET) to measure the vertical concentration profiles of P and Fe in the sediment pore water and in the ditchwater. The Fe concentrations in the sediment pore water ranged between 10 and 200 mg L −1 and exceeded those in the inflowing groundwater by approximately one order of magnitude, due to reductive dissolution of Fe oxyhydroxides in the sediment. The dissolved P concentrations only marginally increased between groundwater and sediment pore water. In the poorly mixed ditchwater, the dissolved Fe concentrations decreased towards the water surface due to oxidative precipitation of fresh Fe oxyhydroxides, and the P concentrations decreased more sharply than those of Fe. These observations support the view that the dynamics of Fe and P are governed by reduction reactions in the sediment and by oxidation reactions in the ditchwater. In the sediment, reductive dissolution of P-containing Fe oxyhydroxides causes more efficient solubilization of Fe than of P, likely because P is buffered by adsorption on residual Fe oxyhydroxides. Conversely, in the ditchwater, oxidative precipitation causes more efficient immobilization of P than of Fe, due to ferric phosphate formation. The combination of these processes yields a natural and highly efficient sink for P. It is concluded that, in Fe-rich systems, the fate of P at the sediment–water interface is determined by reduction and oxidation of Fe. [ABSTRACT FROM AUTHOR]

Abstract: Phosphorus (P) is a limiting nutrient in many aquatic systems. The bioavailability of P in natural waters strongly depends on its speciation. In this study, structural properties of iron colloids were determined and related to their effect on P sorption and P bioavailability. The freshwater green alga Raphidocelis subcapitata was exposed to media spiked with radiolabelled 33PO4, and the uptake of 33P was monitored for 1 h. The media contained various concentrations of synthetic iron colloids with a size between 10 kDa and 0.45 μm. The iron colloids were stabilised by natural organic matter. EXAFS spectroscopy showed that these colloids predominantly consisted of ferrihydrite with small amounts of organically complexed Fe. In colloid-free treatments, the P uptake flux by the algae obeyed Michaelis–Menten kinetics. In the presence of iron colloids at 9 or 90 μM Fe, corresponding to molar P:Fe ratios between 0.02 and 0.17, the truly dissolved P (<10 kDa) was between 4 and 60% of the total dissolved P (<0.45 μm). These colloids reduced the P uptake flux by R. subcapitata compared to colloid-free treatments at the same total dissolved P concentration. However, the P uptake flux from colloid containing solutions equalled that from colloid-free ones when expressed as truly dissolved P. This demonstrates that colloidal P did not contribute to the P uptake flux. It is concluded that, on the short term, phosphate adsorbed to ferrihydrite colloids is not available to the green alga R. subcapitata. [Copyright &y& Elsevier]

Highlights: [•] We studied the formation and transport of iron-rich material in a catchment. [•] As iron-rich groundwaters oxidise, particulate hydrous ferric oxides are formed. [•] This material is transported downstream in the suspended sediment of streams. [•] The iron-rich material strongly sorbs P and may thereby reduce eutrophication risk. [Copyright &y& Elsevier]

Summary: Objective: To examine vocal attack time (VAT) values associated with the production of low, mid, and high rates of vocal fold vibration in normal speakers. Study Design: Sound pressure (SP) and electroglottographic (EGG) recordings were obtained for eight female and five male subjects while producing multiple tokens of the sustained vowels /ɑ/, /i/, and /u/ at comfortable loudness and at mid, low (−3 semitones), and high (+6 semitones) rates of vocal fold vibration. Methods: Generalized sinusoidal models of the SP and EGG signals were computed to compare rates of amplitude change. VAT was computed from the time lag of the cross-correlation function. Results: Adjusted mean VAT for the high frequency condition was smaller than the adjusted mean VAT values for the low- and mid-frequency conditions. There was no significant difference between the mid and low frequency conditions. Conclusions: Findings reveal an association of the VAT measure with increases in vocal fold tension associated with the production of high rates of vocal fold vibration. [Copyright &y& Elsevier]

Summary: Objective: To determine the vocal release time (VRT) for linguistically unconstrained voice offsets in a healthy young adult population. Study Design: Sound pressure (SP) and electroglottographic (EGG) recordings were obtained for 57 female and 55 male subjects while producing multiple tokens of three tasks (sustained /ɑ:/, “always,” and “hallways”) at comfortable pitch and loudness. Methods: SP and EGG signals were digitally time reversed and generalized sinusoidal models of the SP and EGG signals were obtained to compare rates of amplitude change. VRT was computed from the time lag of the cross-correlation function. Results: Adjusted mean VRT values were significantly greater for females than for males. There was no systematic effect of age on VRT. However, 25–29-year old and >40 year old females showed shorter VRT values than the youngest female age group. Conclusions: Normative data are presented for a new measure of the duration of vocal offset, VRT. Acquisition of this measure requires little user intervention, thereby minimizing effects of subjective decision making. Comparison with previously reported vocal attack time (VAT) values for the same population suggests phenomenological differences between linguistically and physiologically constrained voice onsets and unconstrained voice offsets. [Copyright &y& Elsevier]

Summary: Objective: To determine the vocal attack time (VAT) in a healthy young adult population. Study Design: Sound pressure (SP) and electroglottographic (EGG) recordings were obtained for 57 female and 55 male subjects while performing multiple tokens of three tasks (sustained /a/, “always,” and “hallways”) at comfortable pitch and loudness. Methods: Generalized sinusoidal models were obtained for SP and EGG signals to compare rates of amplitude change. VAT was computed from the time lag of the cross-correlation function using a fully automated process accompanied by operator validation. Results: Adjusted mean VAT values were significantly shorter for females than for males. There was no systematic effect of age on VAT. However, 25- to 29-year-old men showed longer VAT than did the other age and sex subgroups. Conclusions: Normative data are presented for a new measure of the duration of vocal initiation, VAT. Acquiring this measure requires very little intervention by the user and, thereby, eliminates the influence of subjective decision making. Although beyond the scope of this article, we suggest several factors that might underlie VAT as a function of speaker sex and age. [ABSTRACT FROM AUTHOR]

Summary: Objectives: To develop and evaluate a figure of merit (FOM) for the measurement of vocal attack time (VAT) for sustained /a/. The FOM assesses a critical assumption of vocal startup on which the VAT measure is based and therefore represents integrity of the derived measure. Methods: Pearson’s correlation coefficient (0≤|r|≤1) was determined from amplitude features of sound pressure (SP) and electroglottographic (EGG) signals (n=1,033 tokens) to serve as a candidate FOM for the study. SP and EGG signals from 102 tokens were visually inspected to empirically derive a criterion level of FOM less than 0.75 to indicate when an assumption underlying a measurement had failed and the VAT measure should be disregarded. Results: The median FOM value for all 1,033 tokens was 0.975. Thirty-two tokens (2.8%) were rejected on the basis of the FOM criterion. There was no correlation between VAT and FOM. Conclusions: A companion metric, Pearson’s r, can be readily obtained to assess the quality of VAT measurement. Decisions to retain or disregard VAT measures may therefore be made on the basis of measurement quality rather than on the basis of group statistics. [Copyright &y& Elsevier]

Summary: The purpose of the present study was to further clarify a definition of belting in physiological and acoustic terms. Twenty professional female singers produced triads ascending by halftone steps from C4 to C5 and sang the last four measures of a popular Broadway ballad in both legit and belt styles. Vocal source differences between belt and legit singing modes were assessed with respect to closed quotient (CQ) and speed quotient (SQ) computed from the electroglottographic waveform during productions of /ma/ triads covering approximately one octave. Differences in supraglottal configuration were evaluated in terms of the ratio (dB) of the amplitudes of the first and second harmonics of /I/ sustained at the pitch , as noted in the long-term average spectrum. CQ did not vary significantly as a function of the singing type. SQ, however, decreased monotonically in association with increasing pitch level in both legit and belt singing styles. Belt mode phonations had higher SQ''s than those of legit mode phonations at the same pitch. A repeated-measures analysis of variance demonstrated that the data differences associated with both pitch level and with singing mode were significant beyond P <0.01. Analysis of the relative magnitude of the first and second harmonics showed that the direction of the harmonic ratio varied between styles. In legit singing style, H1 was always stronger than H2, but this unidirectionality of amplitude was not characteristic of belt singing, for which the second harmonic had greater amplitude in 25% of the cases. Implications of these results are discussed with regard to a new definition of belting. [Copyright &y& Elsevier]

Summary: The objectives of this study were to determine appropriate acoustic and outcome measures for the evaluation of a method of laryngeal manual therapy (LMT) used in the treatment of patients with muscle tension dysphonia (MTD). The effects of this technique were also investigated. The study was based on the hypotheses that the vertical position of the larynx in the vocal tract would lower, that the quality of the voice would normalize, and that a reduction in any vocal tract discomfort (VTD) would occur after LMT. This was a small, prospective, repeated measures pilot study in which each member of the research team was “blinded” to all other stages of the study and during which all data were anonymized until the final stage of data analysis. Ten subjects presenting with MTD completed outcome measures and provided audiorecordings immediately before, immediately after, and 1 week after LMT. The Kay CSL 4150 was used for signal acquisition and for some acoustic measurements. Spectrographic evaluation was accomplished with Praat. A new perceptual, self-rating scale, the VTD scale, and a new proforma for use by the clinician for palpatory evaluation, were developed for the study. Relative average perturbation during connected speech was significantly reduced after LMT, indicating a reduction in abnormal vocal function. The severity and frequency of VTD was shown to have reduced after LMT. This pilot study showed positive evidence for LMT as a method of therapy in the treatment of hyperfunctional voice disorders. Its effects were shown to be measurable with both acoustical analysis and the VTD scale. [Copyright &y& Elsevier]

Summary: The speed with which the vocal folds adduct to the midline is considered an important variable in the etiology of some voice disorders and may also be a meaningful indicator of central or peripheral neural dysfunction. It is proposed that the time lag between the rise of the sound pressure (SP) and electroglottographic (EGG) signals, measured at the onset of phonation, provides a useful index of vocal attack time. This report describes the experimental validation of this measure, whereby the SP and EGG signals were recorded synchronously with high-speed videoendoscopy, from which a digital kymogram was generated. It is shown that, after appropriate signal processing, the intersignal time delay provides a potentially useful measure that varies with vocal attack characteristics. The proposed method calls for no invasive procedures and relies on signals that are routinely obtained in most clinical settings. Unlike acoustic “rise time” measures of voice onset, the glottographic measure involves no operator intervention, requires no arbitrary decisions about measurement points, and may be accomplished quickly and automatically on any personal computer. [Copyright &y& Elsevier]

Abstract: We present a new critical illness VR rehabilitation device (X-VR-D) that enables diversified self-training and is applicable early in the rehabilitation of severely injured or ill patients. The X-VR-D consists of a VR program delivering a virtual scene on a flat screen and simultaneously processing commands to a moving chair mounted on a motion system. Sitting in the moving chair and exposed to a virtual reality environment the device evokes anticipatory and reactive muscle contractions in trunk and extremities for postural control. In this study we tested the device in 10 healthy subjects to evaluate whether the enforced perturbations indeed evoke sufficient and reproducible EMG muscle activations. We found that particular fast roll and pitch movements evoke adequate trunk and leg muscle activity. Higher angular velocities and higher angles of inclination elicited broader EMG bursts and larger amplitudes. The muscle activation pattern was highly consistent between different subjects and although we found some habituation of EMG responses in consecutive training sessions, the general pattern was maintained and was predictable for specific movements. The habituation was characterized by more efficient muscle contractions and better muscle relaxation during the rest positions of the device. Furthermore we found that the addition of a virtual environment to the training session evoked more preparatory and anticipatory muscle activation than sessions without a virtual environment. We conclude that the X-VR-D is safe and effective to elicit consistent and reproducible muscle activity in trunk and leg muscles in healthy subjects and thus can be used as a training method. [Copyright &y& Elsevier]

Summary: The anatomical changes in the senescent larynx are presented, with particular reference to the nonlinearity model of laryngeal biomechanics. The behavior of the Ishizaka–Flanagan 2-mass model in geriatric phonation suggests that an increase in nonlinearity can explain a number of disparate observations about the aged voice. Biomechanical assessment of real laryngeal tissue and experimentation in vivo is needed to confirm the hypothesis, leading to better paradigms of some phonatory disorders and perhaps to novel treatments. [Copyright &y& Elsevier]

Abstract: The murine local lymph node assay (LLNA) was originally developed as a predictive test method for the identification of chemicals with sensitizing potential. In this study we demonstrated that an adapted LLNA can also be used as an immune function assay by studying the effects of orally administered immunomodulating compounds on the T-cell-dependent immune response induced by the contact sensitizer 2,4-dinitrochlorobenzene (DNCB). C57Bl/6 mice were treated with the immunotoxic compounds cyclosporin A (CsA), bis(tri-n-butyltin)oxide (TBTO) or benzo[a]pyrene, (B[a]P). Subsequently, cell proliferation and interferon-γ (IFN-γ) and interleukin (IL)-4 release were determined in the auricular lymph nodes (LNs) after DNCB application on both ears. Immunosuppression induced by CsA, TBTO and B[a]P was clearly detectable in this application of the LLNA. Cytokine release measurements proved valuable to confirm the results of the cell proliferation assay and to obtain an indication of the effect on Th1/Th2 balance. We believe to have demonstrated the applicability of an adapted LLNA as an immune function assay in the mouse. [Copyright &y& Elsevier]

Objective: It is known that P2 cutaneous reflexes from the foot show phase-dependent modulation during gait. The role of the motor cortex and the cortico-spinal tract in these reflexes and their modulation is unknown. Patients with hereditary spastic paraparesis (HSP) have a lesion in the cortico-spinal tract and may show deficits in P2 reflexes and/or their modulation.Methods: Reflex responses of tibialis anterior and biceps femoris after sural nerve stimulation in 10 HSP-patients were compared with those in 10 healthy subjects. The reflexes were studied at two different moments in the step cycle during walking on a treadmill.Results: Both patients and controls showed a phase-dependent modulation of P2 responses. For the individual muscles, no significant difference in reflex activity was observed between HSP-patients and the controls. However, when all muscles were taken together, the reflex activity for the controls was significantly higher than for the patients.Conclusions: The results of this study suggest that the cortico-spinal tract is involved in the regulation of the amplitude of the P2 responses and their phase-dependent modulation. [Copyright &y& Elsevier]

Rolf de Vos (editor of Greenprices) and John Baken (manager of Ecofys) argue that the European focus on large-scale production and infrastructures to solve problems with the security of power supply is counterproductive. Instead of taking the opportunity to go forward and prepare Europe for a future sustainable and reliable power supply by means of decentralised power production units, it will throw us back years. Politicians should look forward and change the conditions in the liberalised market more in favour of decentralisation. [Copyright &y& Elsevier]

The present study explores the ToxCast/Tox21 database to select candidate bioassays as bioanalytical tools for measuring groups of chemicals in water. To this aim, the ToxCast/Tox21 database was explored for bioassays that detect polycyclic aromatic hydrocarbons (PAHs), aromatic amines (AAs), (chloro)phenols ((C)Ps) and halogenated aliphatic hydrocarbons (HAliHs), which are included in the European and/or Dutch Drinking Water Directives. Based on the analysis of the availability and performance of bioassays included in the database, we concluded that several bioassays are suitable as bioanalytical tools for assessing the presence of PAHs and (C)Ps in drinking water sources. No bioassays were identified for AAs and HAliHs, due to the limited activity of these chemicals and/or the limited amount of data on these chemicals in the database. A series of bioassays was selected that measure molecular or cellular effects that are covered by bioassays currently in use for chemical water quality monitoring. Interestingly, also bioassays were selected that represent molecular or cellular effects that are not covered by bioassays currently applied. The usefulness of these newly identified bioassays as bioanalytical tools should be further evaluated in follow-up studies. Altogether, this study shows how exploration of the ToxCast/Tox21 database provides a series of candidate bioassays as bioanalytical tools for measuring groups of chemicals in water. This assessment can be performed for any group of chemicals of interest (if represented in the database), and may provide candidate bioassays that can be used to complement the currently applied bioassays for chemical water quality assessment. [ABSTRACT FROM AUTHOR]

Summary: Vocal attack time (VAT) is the time lag between the growth of the sound pressure signal and the development of physical contact of vocal folds at vocal initiation. It can be derived by a cross-correlation of short-time amplitude changes occurring in the sound pressure and electroglottographic (EGG) signals. Cantonese is a tone language in which tone determines the lexical meaning of the syllable. Such linguistic function of tone has implications for the physiology of tone production. The aim of the present study was to investigate the possible effects of Cantonese tones on VAT. Sound pressure and EGG signals were simultaneously recorded from 59 native Cantonese speakers (31 females and 28 males). The subjects were asked to read aloud 12 disyllabic words comprising homophone pairs of the six Cantonese lexical tones. Results revealed a gender difference in VAT values, with the mean VAT significantly smaller in females than in males. There was also a significant difference in VAT values between the two tone categories, with the mean VAT values of the three level tones (tone 1, 3, and 6) significantly smaller than those of the three contour tones (tone 2, 4, and 5). The findings support the notion that norms and interpretations based on nontone European languages may not be directly applied to tone languages. [Copyright &y& Elsevier]