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Abstract Molecular Communication via Diffusion (MCvD) is a promising paradigm which enables nano-machines to communicate with each other. However, the reliability of existing communication systems degrades rapidly as the distance between the transmitters and the receivers grows. To solve this issue, relaying schemes must be implemented in practice. In this paper, we study two relaying schemes: In the first case, the relay node decodes the incoming signal symbol and forwards it to the receiver using a different types of molecules. Then, the receiver detects the information bits by only considering the molecules from the relay node. In the second case, the receiver considers both types of molecules sent from the transmitter and the relay node. Closed-form expression for the end-to-end bit error probability of the these two schemes are derived. Also, we obtain optimal relay location for both schemes. Moreover, we study the optimal threshold, concentration, baud-rate and timing offset to mitigate the overall Inter-Symbol Interference(ISI) and obtain maximum throughput in the relay network. [ABSTRACT FROM AUTHOR]