1. Denaturing gradient gel electrophoresis fingerprinting of soil bacteria in the vicinity of the Chinese Great Wall Station, King George Island, Antarctica.
- Author
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Pan, Qi, Wang, Feng, Zhang, Yang, Cai, Minghong, He, Jianfeng, and Yang, Haizhen
- Subjects
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DENATURING gradient gel electrophoresis , *DNA fingerprinting , *SOIL microbiology , *SOIL testing , *RIBOSOMAL RNA - Abstract
Bacterial diversity was investigated in soil samples collected from 13 sites around the Great Wall Station, Fildes Peninsula, King George Island, Antarctica, using denaturing gradient gel electrophoresis (DGGE) of 16S rRNA genes. The classes α-, β-, and λ-Proteobacteria, as well as the phylum Actinobacteria, were found to be the dominant bacteria in the soils around the Great Wall Station. Although the selected samples were not contaminated by oil, a relationship between soil parameters, microbial biodiversity, and human impact was still seen. Sample sites in human impacted areas showed lower bacterial biodiversity (average H ′ = 2.65) when compared to non-impacted sites (average H ′ = 3.05). There was no statistically significant correlation between soil bacterial diversity and total organic carbon (TOC), total nitrogen, or total phosphorus contents of the soil. Canonical correlation analysis showed that TOC content was the most important factor determining bacterial community profiles among the measured soil parameters. In conclusion, microbial biodiversity and community characteristics within relatively small scales (1.5 km) were determined as a function of local environment parameters and anthropogenic impact. [ABSTRACT FROM AUTHOR]
- Published
- 2013
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