1. Evaluation of seasonal influenza vaccines for H1N1pdm09 and type B viruses based on a replication-incompetent PB2-KO virus.
- Author
-
Ui, Hiroki, Yamayoshi, Seiya, Uraki, Ryuta, Kiso, Maki, Oishi, Kohei, Murakami, Shin, Mimori, Shigetaka, and Kawaoka, Yoshihiro
- Subjects
- *
SEASONAL influenza , *VIRAL replication , *NEURAMINIDASE , *HEMAGGLUTININ , *IMMUNE response , *VACCINATION - Abstract
Vaccination is the first line of protection against influenza virus infection in humans. Although inactivated and live-attenuated vaccines are available, each vaccine has drawbacks in terms of immunogenicity and safety. To overcome these issues, our group has developed a replication-incompetent PB2-knockout (PB2-KO) influenza virus that replicates only in PB2-expressing cells. Here we generated PB2-KO viruses possessing the hemagglutinin (HA) and neuraminidase (NA) segments from H1N1pdm09 or type B viruses and tested their vaccine potential. The two PB2-KO viruses propagated efficiently in PB2-expressing cells, and expressed chimeric HA as expected. Virus-specific IgG and IgA antibodies were detected in mice immunized with the viruses, and the immunized mice showed milder clinical signs and/or lower virus replication levels in the respiratory tract upon virus challenge. Our results indicate that these PB2-KO viruses have potential as vaccine candidates. [ABSTRACT FROM AUTHOR]
- Published
- 2017
- Full Text
- View/download PDF