1. Gene expression responses of paper birch (Betula papyrifera) to elevated CO2 and O3 during leaf maturation and senescence
- Author
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Kontunen-Soppela, Sari, Parviainen, Juha, Ruhanen, Hanna, Brosché, Mikael, Keinänen, Markku, Thakur, Ramesh C., Kolehmainen, Mikko, Kangasjärvi, Jaakko, Oksanen, Elina, Karnosky, David F., and Vapaavuori, Elina
- Subjects
GENE expression ,PAPER birch ,EFFECT of carbon dioxide on plants ,EFFECT of atmospheric carbon dioxide on plants ,ATMOSPHERIC ozone & the environment ,OZONE & the environment ,EFFECT of ozone on plants ,EFFECT of atmospheric ozone on plants - Abstract
Gene expression responses of paper birch (Betula papyrifera) leaves to elevated concentrations of CO
2 and O3 were studied with microarray analyses from three time points during the summer of 2004 at Aspen FACE. Microarray data were analyzed with clustering techniques, self-organizing maps, K-means clustering and Sammon''s mappings, to detect similar gene expression patterns within sampling times and treatments. Most of the alterations in gene expression were caused by O3 , alone or in combination with CO2 . O3 induced defensive reactions to oxidative stress and earlier leaf senescence, seen as decreased expression of photosynthesis- and carbon fixation-related genes, and increased expression of senescence-associated genes. The effects of elevated CO2 reflected surplus of carbon that was directed to synthesis of secondary compounds. The combined CO2 +O3 treatment resulted in differential gene expression than with individual gas treatments or in changes similar to O3 treatment, indicating that CO2 cannot totally alleviate the harmful effects of O3 . [Copyright &y& Elsevier]- Published
- 2010
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