Search

In present study, a novel glutathione functionalized MoS 2 quantum dots (GSH-MoS 2 QDs) was synthesized from sodium molybdate dehydrate and glutathione by using a one-pot hydrothermal method. After they were characterized, the influence of GSH-MoS 2 QDs on amyloid aggregation of bovine serum albumin (BSA) was investigated by various analytical methods including thioflavin T fluorescence assay, circular dichroism and transmission electron microscope. Moreover, the effect of GSH-MoS 2 QDs on cytotoxicity induced by BSA amyloid fibrils and cell penetration were evaluated by MTT assay and confocal fluorescence imaging, respectively. The results indicated that the GSH-MoS 2 QDs not only had good water solubility, excellent biocompatibility and low cytotoxicity, but also could obviously inhibit the aggregation of BSA and depolymerize the formed BSA aggregates. The data obtained from this work demonstrated that the GSH-MoS 2 QDs is expected to become a candidate drug for the treatment of amyloid-related diseases. • A novel GSH-MoS 2 QDs was synthesized by one-pot hydrothermal approach. • The as-prepared QDs can inhibit BSA aggregation and depolymerize BSA fibrils. • The QDs exhibits good biocompatibility, low cytotoxicity and cell permeability. [ABSTRACT FROM AUTHOR]

[Display omitted] • A novel 4-MPBA-MoS 2 QDs was synthesized by one-pot hydrothermal approach. • The prepared QDs have a uniform spherical appearance with a diameter of 1.5 ∼ 3.0 nm. • Inhibitory effects of the QDs on BSA amyloid aggregation were investigated. • Molecular docking reveals the mechanism of QDs inhibiting BSA amyloid aggregation. In recent years, seeking and screening of compounds that can inhibit and/or depolymerize protein aggregation has been a hot issue in the field of pharmaceutical research. As a new material, quantum dots have been widely concerned by medical researchers. In present study, a novel 4-mercaptophenylboronic acid functionalized MoS 2 quantum dots (4-MPBA-MoS 2 QDs) was successfully synthesized through a one-pot hydrothermal approach by using molybdate dehydrate and 4-mercaptophenylboronic acid as Mo and S source, respectively. Transmission electron microscopy observation showed that the morphology and microstructure of the 4-MPBA-MoS 2 QDs displayed uniform spherical shape with a diameter of approximately 1.5 ∼ 3.0 nm. The UV and fluorescence spectra experiments indicated that the prepared QDs had good water solubility and two weak absorption peaks were appeared at 280 nm and 310 nm. When the excitation wavelength was set to 310 nm, the 4-MPBA-MoS 2 QDs had the strongest fluorescence intensity, and the maximum emission wavelength appeared at 405 nm. In vitro experiments showed that the 4-MPBA-MoS 2 QDs could significantly reduce the aggregation of bovine serum albumin (BSA). Especially when the mass ratio of BSA to 4-MPBA-MoS 2 QDs was 1:5, the inhibition rate could reach 76.4%. Cell experiment showed that the presence of 4-MPBA-MoS 2 QDs could obviously decrease the cytotoxicity induced by BSA amyloid fibrils. Moreover, the depolymerization of BSA amyloid fibrils by 4-MPBA-MoS 2 QDs and its excellent cell permeability were also observed. Molecular docking studies have shown that 4-MPBA-MoS 2 QDs may stabilize the BSA structure through van der Waals forces, hydrophobic force, electrostatic interactions and hydrogen bonds formed between the outer layer of 4-MPBA and BSA to prevent fibrosis aggregation. The results of this study suggested that 4-MPBA-MoS 2 QDs showed low cytotoxicity, good biocompatibility and solubility, and had a great potential in the design of new drugs for the treatment of amyloid-related diseases. [ABSTRACT FROM AUTHOR]

Abstract In this study, cysteamine-functionalized molybdenum disulfide quantum dots (MoS 2 QDs) were synthesized by a one-pot hydrothermal method. A range of techniques including of Thioflavin T and 8-Anilino-1-naphthalenesulfonic acid fluorescence assays, circular dichroism, and transmission electron microscope have been employed to determination the efficacy of MoS 2 QDs on the inhibition/reversion of fibrillation and hindering cytotoxicity induced by protofibrils and amyloid fibrils of bovine serum albumin (BSA). Results demonstrated that MoS 2 QDs could effectively inhibit the fibrillogenesis and destabilize preformed fibrils of BSA in a concentration-dependent manner. Cytotoxicity protection and imagine on Hela cells was investigated using the methyl thiazolyl tetrazolium (MTT) assay. It was found that MoS 2 QDs not only has good biocompatibility, low toxicity and good cell penetration, but also could effectively decrease the cytotoxicity caused by the formed fibrils of BSA. The results obtained in this work suggested the potential biological application of MoS 2 QDs in therapeutics and provided new insight into the design of multifunctional nanomaterials for amyloid-related diseases. [ABSTRACT FROM AUTHOR]

In this study, the effect of a glycoprotein obtained from Fupenzi (FPZ) (Rubus chingii Hu.) on the fibrillation of bovine serum album (BSA) was investigated by multi-spectroscopic methods and transmission electron microscopy. Moreover, the cytotoxicity of the glycoprotein and the effect of it on H 2 O 2 -induced cell viability were investigated by cell counting kit and β-galactosidase kit, respectively. The experimental results indicated that the glycoprotein showed very low toxicity to NRK-52E cells and could obviously delay cell senescence and improve cell viability. Moreover, the glycoprotein could effectively inhibit the formation of BSA fibrils and destroy the stability of preformed BSA fibrils in a concentration-dependent manner. Generally, antioxidant capacities are thought to be related to the anti-amyloidogenic activity of inhibitors; therefore, to reveal the inhibitory mechanism, the anti-oxidative property of the glycoprotein was examined by DPPH and ABTS assays. The results demonstrated that FPZ glycoprotein had a remarkable antioxidant activity and the IC 50 values of DPPH and ABTS were 0.249 mg mL−1 and 0.092 mg mL−1, respectively. This work suggested that the FPZ glycoprotein had the potential to be designed a new therapeutic agent for attenuating aging and preventing the age-related diseases. Transmission electron microscopy images of BSA fibril. A: a flat-ribbon and long-rod-shaped, self-assembled network fibrils were appeared after the native BSA solution was incubated at 65 °C for 8 h. B: under the same experimental conditions, the amounts of fibrils were obviously reduced in the presence of 1.0 mg mL−1 glycoprotein. Unlabelled Image • Effect of a novel FPZ glycoprotein on fibrillation of BSA was investigated. • Toxicity of glycoprotein and its effect on H 2 O 2 -induced cell viability were tested. • Anti-oxidative property of glycoprotein was examined by DPPH and ABTS assays. [ABSTRACT FROM AUTHOR]