1. Performance of a clonal-based HIV-1 tropism phenotypic assay.
- Author
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Asin-Milan, Odalis, Wei, Yi, Sylla, Mohamed, Vaisheva, Farida, Chamberland, Annie, and Tremblay, Cécile L.
- Subjects
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CLONE cells , *HIV , *VIRAL tropism , *PHENOTYPES , *GENETIC vectors , *GENE transfection - Abstract
Highlights: [•] A clonal-based HIV-1 tropism phenotypic assay was developed. [•] Env-pseudotyped viruses were produced by co-transfection of the env-pcDNA3.1/V5HisTOPO expression vector and a backbone pNL4-3.Luc.E-R- in 293T cell cultures. [•] Coreceptor use was tested by infection of U87-CD4-CCR5+ and U87-CD4-CXCR4+ cells. [•] This sensitive clonal based phenotypic assay was able to differentiate between the truly dual-tropic viruses and a mixture of R5- and X4-tropic viruses. [•] Minor variants were always detectable when present at 0.4% or greater in the mixtures. [ABSTRACT FROM AUTHOR]
- Published
- 2014
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