1. Comparative performance of WANTAI ELISA for total immunoglobulin to receptor binding protein and an ELISA for IgG to spike protein in detecting SARS-CoV-2 antibodies in Kenyan populations.
- Author
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Nyagwange, James, Kutima, Bernadette, Mwai, Kennedy, Karanja, Henry K., Gitonga, John N., Mugo, Daisy, Uyoga, Sophie, Tuju, James, Ochola-Oyier, Lynette I., Ndungu, Francis, Bejon, Philip, Agweyu, Ambrose, Adetifa, Ifedayo M.O., Scott, J.Anthony G., and Warimwe, George M.
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IMMUNOGLOBULIN receptors , *CARRIER proteins , *SARS-CoV-2 , *PROTEIN receptors , *IMMUNOGLOBULIN G , *KILLER cell receptors , *VIRAL antibodies - Abstract
• Both assays discriminate SARS-CoV-2 seropositive and seronegative individuals. • Both assays correctly estimate seroprevalence in a wide range of seroprevalences. • WANTAI has slightly higher sensitivity than KWTRP. • KWTRP has slightly higher specificity than WANTAI. Many SARS-CoV-2 antibody detection assays have been developed but their differential performance is not well described. In this study we compared an in-house (KWTRP) ELISA which has been used extensively to estimate seroprevalence in the Kenyan population with WANTAI, an ELISA which has been approved for widespread use by the WHO. Using a wide variety of sample sets including pre-pandemic samples (negative gold standard), SARS-CoV-2 PCR positive samples (positive gold standard) and COVID-19 test samples from different periods (unknowns), we compared performance characteristics of the two assays. The overall concordance between WANTAI and KWTRP was 0.97 (95% CI, 0.95–0.98). For WANTAI and KWTRP, sensitivity was 0.95 (95% CI 0.90–0.98) and 0.93 (95% CI 0.87–0.96), respectively. Specificity for WANTAI was 0.98 (95% CI, 0.96–0.99) and 0.99 (95% CI 0.96–1.00) while KWTRP specificity was 0.99 (95% CI, 0.98–1.00) and 1.00 using pre-pandemic blood donors and pre-pandemic malaria cross-sectional survey samples respectively. Both assays show excellent characteristics to detect SARS-CoV-2 antibodies. [ABSTRACT FROM AUTHOR]
- Published
- 2022
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