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To enhance the cellulose digestibility of energy grass hybrid Pennisetum ( P. americanum × P. purpureum , HP) with low energy-consumption and high efficiency, a novel combinatorial pretreatment of oxygen-aqueous alkaline ionic liquid (IL) 1-ethyl-3-methylimidazolium acetate ([Emim]Ac) media (OEA) was developed in this work. The combinatorial pretreatment was performed under the relatively low temperature (120 °C), short retention time (30 min), and 12 bar oxygen pressure. The combinatorial pretreatment of OEA was demonstrated effectively for pretreatment of hybrid Pennisetum , which evidenced by the removal of lignin, degradation of carbohydrate, and porosity property of the regenerated biomass. Subsequently, a higher glucose recovery (96.9%) at a low enzyme loading (20 FPU/g substrate) was obtained by the OEA pretreatment, and it was 9.1 times as much as the untreated samples. Overall, the novel OEA combinatorial pretreatment has the advantages of low thermal energy input and enzyme usage, and short retention time. [ABSTRACT FROM AUTHOR]

Biofuel production from lignocellulose has recently been gaining much more attention as a result. One major problem of using lignocellulosic materials for the production of biofuel is the low accessibility of cellulose to enzymes and microorganisms. Therefore, pretreatment of lignocellulose is a critical step in biofuel production from such materials. Of the pretreatments, fungal treatment has become an important process due to its low energy demands and selective degradation of lignin and hemicellulose. This capability comes from the unique enzymatic systems, cellulolytic and ligninolytic enzymes, especially in white rot fungi. The low energy demand of fungal pretreatment has generated interest in studying the applicability of fungal pretreatment for biofuel production from woody materials. The most significant drawback of fungal pretreatment is the lengthy time required for the process. Combining fungal pretreatment with other pretreatment methods might reduce the time necessary for the whole process to operate. It can also introduce cost-effectiveness. Thus combining fungal pretreatment with other physical and chemical methods has been recently contemplated. This paper provides a comprehensive review of current fungal pretreatments and feasibility for biofuel production, with a focus on combining fungal pretreatment with other methods. The advantages and disadvantages of all physical and chemical methods were also briefly reviewed. The applicability of the combination of fungal with other pretreatment methods has been considered in a number of recent publications. To be commercially attractive, both energy demand and processing time should be reduced. In terms of energy demand reduction, combined fungal physico-chemical pretreatment has been effective. However, the lengthy time taken for the whole process has not been significantly improved upon. A great deal of work is still required to be done regarding time reduction for the process (combined fungal-physico chemical pretreatment). Therefore, it seems to remain an open field for research and process development. [ABSTRACT FROM AUTHOR]

Combined pretreatment with hot compressed water and wet disk milling was performed with the aim to reduce the natural recalcitrance of oil palm biomass by opening its structure and provide maximal access to cellulase attack. Oil palm empty fruit bunch and oil palm frond fiber were first hydrothermally pretreated at 150–190 °C and 10–240 min. Further treatment with wet disk milling resulted in nanofibrillation of fiber which caused the loosening of the tight biomass structure, thus increasing the subsequent enzymatic conversion of cellulose to glucose. The effectiveness of the combined pretreatments was evaluated by chemical composition changes, power consumption, morphological alterations by SEM and the enzymatic digestibility of treated samples. At optimal pretreatment process, approximately 88.5% and 100.0% of total sugar yields were obtained from oil palm empty fruit bunch and oil palm frond fiber samples, which only consumed about 15.1 and 23.5 MJ/kg of biomass, respectively. [ABSTRACT FROM AUTHOR]

Pretreatment was the essential step for industrial application of lignocellulosic biomass. Combination of steam explosion and fungal treatment was conducted, and synergistic mechanism of the combined pretreatment was evaluated in terms of pore size distribution, crystallinity index, chemical compositions and enzymatic hydrolysis. The results showed that steam explosion destroyed the rigid structure of corn stalk, increased pore size and porosity, and exposed crystalline component of cellulose. Steam explosion broke the lignin-carbohydrate-complex structure of lignocellulosic biomass and facilitated the fungal treatment.Phellinus baumii could selectively degrade 34.7% and 36.58% of lignin for 1.4 MPa and 1.7 MPa steam-exploded corn stalk, respectively. As a result, the highest glucose yield of corn stalk pretreated by the condition of 1.7 MPa steam explosion associated with 21 d P. baumii reached 313.31 g kg-1, which was 2.88 and 1.32 times higher than that of the untreated corn stalk and the 1.7 MPa steam-exploded corn stalk, respectively. The combined pretreatment enhanced the enzymatic hydrolysis, which was a promising technology that might be explored as alternative to the existing pretreatment. [ABSTRACT FROM AUTHOR]

[Display omitted] • Additives in pretreatment notably raised fermentable sugars and bioethanol yields. • Mechanisms of reducing lignin repolymerization by additives were investigated. • −OH groups of additives suppressed lignin repolymerization by forming a new reaction. • More hydrophilic groups of lignin reduced non-specific binding into enzymes. • Enlarged biomass porosity and lower surface coverage reduced surface lignin barrier. Pretreatment is the key factor for producing fermentable sugars and biofuels from lignocellulosic biomass. However, during pretreatment lignin repolymerization occurs which reduces the efficiency of carbohydrates hydrolysis. In the present study, three additives (2-naphthol-7-sulfonate, 2-naphthol, and mannitol) were evaluated for boosting the effectiveness of combined pretreatment (liquid hot water + green liquor) of pine-wood biomass. The results revealed that supplied additives into combined pretreatment changed the physicochemical structure of lignin, therefore, alleviating the inhibitory effect of lignin repolymerization and notably raised sugars yield (glucose, xylose) ranging from 85% to 93%. XPS and FTIR analyses confirmed that −OH groups of additives led to lower formation of phenolic hydroxyl (PhOH) and higher hydrophilic groups of lignin, accounting for reduced non-specific binding of lignin into cellulase enzymes. Furthermore, additives increased surface lignin coverage, biomass porosity, and fiber swelling by 1.96–2.59, 1.66–1.86, and 1.13–1.29 folds respectively, comparing to corresponding sample without additives caused more liquified and extractable lignin, which notably reduced the surface lignin barrier. Hence, both factors including non-specific binding effect and surface lignin barrier, contributed to reducing lignin repolymerization, thereby increased cellulose accessibility for high production of fermentable sugars and bioethanol in softwood biomass. [ABSTRACT FROM AUTHOR]

Abstract: In this study, individual pretreatments (acid and ultrasonic) and a combination of these pretreatments were optimized to enhance the efficiency of dark fermentative hydrogen production (DFHP) from microalgal biomass. The experimental results show that the maximum H2 production performance of 42.1mL H2/g dry cell weight (dcw) was predicted at 0.79% (v/w) HCl and at a specific energy input (SEI) of 49,600kJ/kg dcw in the combined pretreatment, while it was limited in both individual pretreatments. Repeated batch testing of the predicted optimal conditions revealed that the combined pretreatment conditions for DFHP from microalgal biomass were successfully optimized by increasing the solubilization of the feedstock and by reducing the formation of the toxic 5-hydroxymethylfurfural (HMF). [Copyright &y& Elsevier]

Abstract: Pretreatment technology is a prerequisite to facilitate the release of sugars from a lignocellulosic biomass prior to fermentation. Recently, some pretreatment methods have been tried with ionic liquids, but they were still expensive and unpractical. In this study, an efficient pretreatment method using ammonia and ionic liquid was developed for the recovery of bio-digestible cellulose from a lignocellulosic byproduct, rice straw, and the increase of ionic liquid utilization. The combined use of ammonia and ionic liquid ([Emim]Ac) treatment exhibited a synergy effect for rice straw with 82% of the cellulose recovery and 97% of the enzymatic glucose conversion. This cooperative effect showed over 90% of the glucose conversion even with a reduced enzyme usage and incubation time. The ionic liquid was successfully recycled more than 20 times. The 20th-recycled ([Emim]Ac) showed 74% of the cellulose recovery and 78% of the glucose conversion to rice straw. Compared with the conventional pretreatment, our combined method for lignocellulosic biomass pretreatment was an economical and eco-friendly. [Copyright &y& Elsevier]

• Pretreatment is an essential step for the breakdown of lignocellulosic biomass. • Combining pretreatment helps to lower the severity of the drawbacks of a single step. • Biological-chemical/physicochemical combined pretreatment strategies were reviewed. • The order of pretreatment in the combined studies affects the overall efficiency. Sustainable biorefinery concepts based on lignocellulosic biomass are gaining worldwide research interest because of their inexpensiveness and abundance. The recalcitrance of lignocellulosic biomass poses a major hindrance to enhance biofuel production. Therefore, a pretreatment step is critical to prepare the substrates for the downstream process. Combining pretreatment steps help to lower the severity of the drawbacks of a single pretreatment step. This paper systematically reviews the combined biological and chemical/physicochemical pretreatment based on fiber degradation and sugar yield. An energy-efficient biological pretreatment method combined with a chemical pretreatment that accelerates the pretreatment times has been seen to be efficient for fiber degradation and sugar yields. However, fungal species, culture conditions, biomass type, the severity of chemical pretreatment and the order of sequential pretreatment influences the relative component contents and sugar yield. Even the same biomass from different sources undergoing similar pretreatment conditions could result in a varying amount of digestibility. [ABSTRACT FROM AUTHOR]