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• Protein concentrate production conditions were optimized. • Genotype effected the features of protein concentrates. • Some genotypes exhibited superior technological properties. • Color effected the bioactive features. In this study, a comparative investigation on the structural, technofunctional and bioactive properties of protein concentrates from different common bean genotypes was performed. Protein extractions were carried out at different pH and salt concentrations and the highest protein content for the concentrates (77.7%) was determined for pH 11 and 0.4% of salt. The protein content of the common bean flour and their protein concentrates was in the range of 22–26.93% and 72.97–77.99%, respectively. For bioactive properties, total phenolic content ranged between 578.9 and 1355.9 and 313.5–1219.1 mg GAE/kg, for bean flours and protein concentrates, respectively. Two genotypes (G7 and G8) were the samples showing the superior biofunctional properties compared to the others. Thermal characterization showed that T d and ΔH values were in the range of 64.95–94.33 °C and 76.64–122.3 j/g, respectively. SDS-PAGE analysis revealed that the major band corresponded to the 7S vicilin. Principal component analysis showed that G2 and G6 had different characteristics in terms of technofunctional parameters while G7 and G8 were differed from the other genotypes in terms of bioactivity. The results showed that the proteins of common beans could be evaluated as good source due to high bioactivity for the enrichment of food formulations. [ABSTRACT FROM AUTHOR]

In the current study, the effect of glycerol -as a green solvent- addition into solvent mixture (50:50 ethanol:distilled water), on some biofunctional properties of Origanum onites L. was investigated. Response surface methodology (RSM) was used to detect the optimum conditions for the extraction process. Three variables namely glycerol concentration (X 1 : 1–9 g), extraction temperature (X 2 : 25–75 °C) and time (X 3 :10–30 min) were selected and also total phenolic content, total flavonoid content, antioxidant capacity and antiradical activity of O. onites extracts were determined. Analysis of variance (ANOVA) showed that glycerol incorporation significantly increased the total phenolic content and antioxidant activity (p < 0.05) of the samples. Maximum levels to obtain the highest bioactive properties (highest total phenolic content and antioxidant activity) were determined as to be 9 g of glycerol addition for the extraction conditions as 45.4 °C and 75 min. This study reports the effect of glycerol on bioactive properties of O. onites and suggests that glycerol can be used to produce hydroalcoholic extracts having higher bioactivity from Origanum genus. [ABSTRACT FROM AUTHOR]

• Capsicum annuum (Long pepper) × Capsicum frutescens (PI281420) were selfed. • Total phenolics, flavonoid and total anthocyanin contents of the red pepper lines changed significantly. • Scoville heat unit revealed that the 95% of the peppers were highly pungent. • The major phenolics of peppers were detected as p-coumaric acid. In this study, 104 RIL (Recombinant Inbred Pepper Lines: F6) populations which generated by selfing Capsicum annuum (Long pepper) × Capsicum frutescens (PI281420) F6 population were characterized in terms of detailed bioactive properties, major phenolic composition, tocopherol and capsaicinoid profile. Total phenolics, flavonoid and total anthocyanin contents of the red pepper lines were in the range of 7.06–17.15 mg gallic acid equivalent (GAE)/g dw, 1.10–5.46 mg catechin equivalent (CE)/g dw and 7.9–516.6 mg/kg dw extract, respectively. Antiradical activity and antioxidant capacity values also ranged between 18.99 and 49.73% and 6.97–16.47 mg ascorbic acid equivalent (AAE)/kg dw, respectively. Capsaicin and dihydrocapsaicin levels showed a wide variance with the range of 27.9–1405.9 and 12.3–640.4 mg/100 g dw, respectively. Scoville heat unit revealed that the 95% of the peppers were highly pungent. The major tocopherol was alpha tocopherol for the pepper samples with the highest level of 1078.4 µg/g dw. The major phenolics were detected as p-coumaric acid, ferulic acid, myricetin, luteolin and quercetin. Pepper genotypes showed significant differences in terms of the characterized properties and principal component analysis was applied successfully to reveal the similar genotypes. [ABSTRACT FROM AUTHOR]

Thirty-three different Hypericum perforatum genotypes were characterized in terms of morphological (plant height, herbage yields), physicochemical (ash, cellulose, protein, and colour), and bioactive properties. The antioxidant and antibacterial capacity were also assessed. All genotype samples were characterized as flower part and whole plant (aerial part). It was observed that the plant height ranged between 21.6 and 96.6 cm and whole plant drog yield was in the range of 11–146 g. Bioactivity showed wide distribution for the samples and all bioactive parameter values were measured as lower for the whole plant compared to the flower part. Total phenolic content ranged between 60.39-110.54 and 49.08–89.53 mg GAE/g for the flower part and whole plant, respectively. Flower extracts also showed good inhibition activity against some Gram (+) bacteria. The GT-Biplot statistical mathematical classification approach was applied to reveal the promising genotypes. • Hypericum perforatum genotypes were morphologically investigated. • The antioxidant and antibacterial capacity were also assessed. • Extracts from flower parts showed higher bioactivity than those from whole plants. • Flower extracts inhibited some Gram (+) bacteria. • GT-Biplot approach was applied to unravel the promising genotypes. [ABSTRACT FROM AUTHOR]